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Protein

Ribonuclease pancreatic

Gene

RNASE1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single-stranded and double-stranded RNA.1 Publication

Miscellaneous

Ribonuclease can destabilize or unwind the DNA helix by complexing with single-stranded DNA; this complex arises by an extended multisite cation-anion interaction between the lysine and arginine residues of the enzyme and the phosphate groups of the nucleotides.

Catalytic activityi

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33Substrate1
Binding sitei36Substrate1
Active sitei38Proton acceptor1
Binding sitei92Substrate1
Binding sitei111Substrate1
Active sitei145Proton donor1

GO - Molecular functioni

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease

Enzyme and pathway databases

BRENDAi3.1.27.5. 908.
SABIO-RKiP61823.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease pancreatic (EC:3.1.27.5)
Alternative name(s):
RNase 1
RNase A
Gene namesi
Name:RNASE1
Synonyms:RNS1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 10

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi38H → A: Loss of activity. 1
Mutagenesisi145H → A: Loss of activity. 1

Chemistry databases

ChEMBLiCHEMBL6091.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 262 PublicationsAdd BLAST26
ChainiPRO_000003091527 – 150Ribonuclease pancreaticAdd BLAST124

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi27N-linked (Glc) (glycation) lysine; in vitro1
Glycosylationi33N-linked (Glc) (glycation) lysine; in vitro1
Disulfide bondi52 ↔ 1101 Publication
GlycosylationiCAR_00000660N-linked (GlcNAc...) asparagine; partial1 Publication1
Glycosylationi63N-linked (Glc) (glycation) lysine; in vitro1
Disulfide bondi66 ↔ 1211 Publication
Glycosylationi67N-linked (Glc) (glycation) lysine; in vitro1
Disulfide bondi84 ↔ 1361 Publication
Disulfide bondi91 ↔ 981 Publication

Keywords - PTMi

Disulfide bond, Glycation, Glycoprotein

Proteomic databases

PaxDbiP61823.
PeptideAtlasiP61823.
PRIDEiP61823.

PTM databases

UniCarbKBiP61823.

Miscellaneous databases

PMAP-CutDBiP61823.

Expressioni

Tissue specificityi

Pancreas.

Gene expression databases

BgeeiENSBTAG00000008793.

Interactioni

Subunit structurei

Interacts with and forms tight 1:1 complexes with RNH1. Dimerization of two such complexes may occur. Interaction with RNH1 inhibits this protein (By similarity). Monomer.HMP:1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

DIPiDIP-36384N.
IntActiP61823. 7 interactors.
MINTiMINT-1510416.
STRINGi9913.ENSBTAP00000011586.

Chemistry databases

BindingDBiP61823.

Structurei

Secondary structure

1150
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi30 – 38ND:9
Beta strandi43 – 45ND:3
Helixi48 – 50ND:3
Helixi51 – 58ND:8
Beta strandi61 – 65ND:5
Beta strandi68 – 73ND:6
Helixi77 – 82ND:6
Helixi83 – 85ND:3
Beta strandi86 – 89ND:4
Beta strandi92 – 94ND:3
Beta strandi98 – 100ND:3
Beta strandi102 – 112ND:11
Beta strandi118 – 120ND:3
Beta strandi123 – 130ND:8
Beta strandi132 – 137ND:6
Turni138 – 141ND:4
Beta strandi142 – 149ND:8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A2WX-ray2.10A/B27-150[»]
1A5PX-ray1.60A27-150[»]
1A5QX-ray2.30A27-150[»]
1AFKX-ray1.70A/B27-150[»]
1AFLX-ray1.70A/B27-150[»]
1AFUX-ray2.00A/B27-150[»]
1AQPX-ray2.00A27-150[»]
1B6VX-ray2.00A/B27-150[»]
1BELX-ray1.60A27-150[»]
1BZQX-ray2.80A/B/C/D27-150[»]
1C0BX-ray1.90A23-150[»]
1C0CX-ray2.00A23-150[»]
1C8WX-ray1.80A27-150[»]
1C9VX-ray1.70A27-150[»]
1C9XX-ray1.80A27-150[»]
1CJQX-ray3.00A27-41[»]
B50-150[»]
1CJRX-ray2.30A27-41[»]
B50-150[»]
1D5DX-ray2.25A27-41[»]
B50-150[»]
1D5EX-ray2.25A27-41[»]
B50-150[»]
1D5HX-ray2.25A27-41[»]
B50-150[»]
1DFJX-ray2.50E27-150[»]
1DY5X-ray0.87A/B27-150[»]
1EICX-ray1.40A27-150[»]
1EIDX-ray1.40A27-150[»]
1EIEX-ray1.40A27-150[»]
1EOSX-ray2.00A/B27-150[»]
1EOWX-ray2.00A27-150[»]
1F0VX-ray1.70A/B/C/D27-150[»]
1FEVX-ray2.25A27-41[»]
B50-150[»]
1FS3X-ray1.40A27-150[»]
1GV7X-ray2.10A84-99[»]
1IZPX-ray1.50A27-150[»]
1IZQX-ray1.80A27-150[»]
1IZRX-ray1.50A27-150[»]
1J7ZX-ray2.25A27-41[»]
B47-150[»]
1J80X-ray2.10A27-41[»]
B47-150[»]
1J81X-ray2.20A27-41[»]
B47-150[»]
1J82X-ray2.30A27-41[»]
B47-150[»]
1JN4X-ray1.80A/B27-150[»]
1JS0X-ray2.20A/B/C27-150[»]
1JVTX-ray2.05A/B27-150[»]
1JVUX-ray1.78A/B27-150[»]
1JVVX-ray2.20A/B27-150[»]
1KF2X-ray1.10A27-150[»]
1KF3X-ray1.05A27-150[»]
1KF4X-ray1.10A27-150[»]
1KF5X-ray1.15A27-150[»]
1KF7X-ray1.15A27-150[»]
1KF8X-ray1.15A27-150[»]
1KH8X-ray2.00A27-150[»]
1LSQX-ray1.90A/B27-150[»]
1O0FX-ray1.50A/B27-150[»]
1O0HX-ray1.20A/B27-150[»]
1O0MX-ray1.50A/B27-150[»]
1O0NX-ray1.50A/B27-150[»]
1O0OX-ray1.20A/B27-150[»]
1QHCX-ray1.70A/B27-150[»]
1RARX-ray1.90A28-150[»]
1RASX-ray1.70A28-150[»]
1RATX-ray1.50A27-150[»]
1RBBX-ray2.50A/B27-150[»]
1RBCX-ray2.00A47-150[»]
S27-41[»]
1RBDX-ray1.70A47-150[»]
S27-41[»]
1RBEX-ray1.75A47-150[»]
S27-41[»]
1RBFX-ray1.80A47-150[»]
S27-41[»]
1RBGX-ray1.80A47-150[»]
S27-41[»]
1RBHX-ray1.70A47-150[»]
S27-41[»]
1RBIX-ray1.80A47-150[»]
S27-41[»]
1RBJX-ray2.70A27-150[»]
1RBNX-ray2.10A27-150[»]
1RBWX-ray1.69A27-150[»]
1RBXX-ray1.69A27-150[»]
1RCAX-ray1.90A27-150[»]
1RCNX-ray2.32E27-150[»]
1RHAX-ray1.80A27-150[»]
1RHBX-ray1.50A27-150[»]
1RNCX-ray1.50A27-150[»]
1RNDX-ray1.50A27-150[»]
1RNMX-ray2.00E27-150[»]
1RNNX-ray1.80E27-150[»]
1RNOX-ray1.90A27-150[»]
1RNQX-ray2.00A27-150[»]
1RNUX-ray1.60A27-150[»]
1RNVX-ray1.60A27-150[»]
1RNWX-ray1.80A27-150[»]
1RNXX-ray1.90A27-150[»]
1RNYX-ray2.00A27-150[»]
1RNZX-ray1.90A27-150[»]
1ROBX-ray1.60A27-150[»]
1RPFX-ray2.20A27-150[»]
1RPGX-ray1.40A27-150[»]
1RPHX-ray2.20A27-150[»]
1RSMX-ray2.00A27-150[»]
1RTAX-ray2.50E27-150[»]
1RTBX-ray2.50A27-150[»]
1RUVX-ray1.25A27-150[»]
1SRNX-ray1.80A27-144[»]
B137-150[»]
1SSAX-ray2.00A27-144[»]
B137-150[»]
1SSBX-ray2.00A27-144[»]
B137-150[»]
1SSCX-ray2.00A27-138[»]
B140-150[»]
1U1BX-ray2.00A/B27-150[»]
1UN5X-ray2.60A64-74[»]
1W4OX-ray1.60A/B27-150[»]
1W4PX-ray1.69A/B27-150[»]
1W4QX-ray1.68A/B27-150[»]
1WBUX-ray1.90A/B27-150[»]
1XPSX-ray1.80A/B27-150[»]
1XPTX-ray1.90A/B27-150[»]
1YMNX-ray1.45A27-150[»]
1YMRX-ray1.50A27-150[»]
1YMWX-ray1.50A27-150[»]
1Z3LX-ray1.80E47-150[»]
S27-41[»]
1Z3MX-ray2.00E47-150[»]
S27-41[»]
1Z3PX-ray2.00E47-150[»]
S27-41[»]
1Z6DX-ray1.54A/B27-150[»]
1Z6SX-ray1.50A/B27-150[»]
2AASNMR-A27-150[»]
2APQX-ray1.80A27-150[»]
2APUmodel-0/1/2/3/A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d/e/f/g/h/i/j/k/l/m/n/o/p/q/r/s/t/u/v/w/x/y/z27-150[»]
2BLPX-ray1.40A27-150[»]
2BLZX-ray1.40A27-150[»]
2E33X-ray2.70B27-150[»]
2E3WX-ray1.05A27-150[»]
2G4WX-ray1.84A/B27-150[»]
2G4XX-ray1.95A27-150[»]
2G8QX-ray1.50A/B27-150[»]
2G8RX-ray1.70A/B27-150[»]
2NUIX-ray1.10A27-150[»]
2OP2X-ray1.60A27-150[»]
2OQFX-ray2.30A/B/C/D27-150[»]
2P42X-ray1.80A/C27-150[»]
2P43X-ray1.65A27-150[»]
2P44X-ray1.80A27-150[»]
2P45X-ray1.10A27-150[»]
2P46X-ray2.50A/C27-150[»]
2P47X-ray2.50A27-150[»]
2P48X-ray2.30A27-150[»]
2P49X-ray1.38A27-150[»]
2P4AX-ray1.90A/C27-150[»]
2QCAX-ray1.33A27-150[»]
2RATX-ray1.50A27-150[»]
2RLNX-ray1.85E42-150[»]
S27-41[»]
2RNSX-ray1.60A27-150[»]
2W5GX-ray1.70A/B27-150[»]
2W5IX-ray2.40A/B27-150[»]
2W5KX-ray1.70A/B27-150[»]
2W5LX-ray1.70A/B27-150[»]
2W5MX-ray1.80A/B27-150[»]
2XOGX-ray1.72A/B27-150[»]
2XOIX-ray1.72A/B27-150[»]
3A1Rneutron diffraction1.70A27-150[»]
3D6OX-ray1.58A/B27-150[»]
3D6PX-ray1.60A/B27-150[»]
3D6QX-ray1.60A/B27-150[»]
3D7BX-ray1.60A/B27-150[»]
3D8YX-ray1.72A/B27-150[»]
3D8ZX-ray1.98A/B27-150[»]
3DH5X-ray1.60A27-150[»]
3DH6X-ray1.60A27-150[»]
3DI7X-ray1.60A27-150[»]
3DI8X-ray1.60A27-150[»]
3DI9X-ray2.00A27-150[»]
3DIBX-ray1.40A27-150[»]
3DICX-ray1.60A27-150[»]
3DXGX-ray1.39A/B27-150[»]
3DXHX-ray1.40A/B27-150[»]
3EUXX-ray1.65A/B27-150[»]
3EUYX-ray1.95A/B27-150[»]
3EUZX-ray1.84A/B27-150[»]
3EV0X-ray1.76A/B27-150[»]
3EV1X-ray2.00A/B27-150[»]
3EV2X-ray2.02A/B27-150[»]
3EV3X-ray1.68A/B27-150[»]
3EV4X-ray1.93A/B27-150[»]
3EV5X-ray1.68A/B27-150[»]
3EV6X-ray1.76A/B27-150[»]
3FKZX-ray1.99A/B27-150[»]
3FL0X-ray1.94A/B27-150[»]
3FL1X-ray1.90A/B27-150[»]
3FL3X-ray1.60A/B27-150[»]
3I67X-ray1.30A27-150[»]
3I6FX-ray1.30A27-150[»]
3I6HX-ray1.30A27-150[»]
3I6JX-ray1.30A27-150[»]
3I7WX-ray2.35A27-150[»]
3I7XX-ray2.60A27-150[»]
3I7YX-ray2.40A27-150[»]
3JW1X-ray1.60A/B27-150[»]
3LXOX-ray1.55A27-150[»]
3MWQX-ray1.68A26-150[»]
3MWRX-ray1.85A27-150[»]
3MX8X-ray2.10A26-150[»]
3MZQX-ray1.50A23-150[»]
3MZRX-ray1.50A23-150[»]
3OQYX-ray1.49A/B47-150[»]
a/b27-41[»]
3OQZX-ray2.50A/B47-150[»]
a/b27-41[»]
3OR0X-ray2.30A/B47-150[»]
a/b27-41[»]
3QL1X-ray1.29A27-150[»]
3QL2X-ray1.49A/B27-150[»]
3QSKX-ray1.75A27-150[»]
3RATX-ray1.50A27-150[»]
3RH1X-ray2.10A27-150[»]
3RIDX-ray2.18A/B/C/D27-150[»]
3RN3X-ray1.45A27-150[»]
3RSDX-ray1.60A27-150[»]
3RSKX-ray2.00A27-150[»]
3RSPX-ray1.70A27-150[»]
3SRNX-ray2.00A27-139[»]
B140-146[»]
4AO1X-ray1.58A27-150[»]
4G8VX-ray1.70A/B27-150[»]
4G8YX-ray1.80A/B27-150[»]
4G90X-ray1.90A/B27-150[»]
4J5ZX-ray1.80A27-150[»]
4J60X-ray1.69A27-150[»]
4J61X-ray1.69A27-150[»]
4J62X-ray2.04A27-150[»]
4J63X-ray2.04A27-150[»]
4J64X-ray1.78A27-150[»]
4J65X-ray1.96A27-150[»]
4J66X-ray2.04A27-150[»]
4J67X-ray1.86A27-150[»]
4J68X-ray1.78A27-150[»]
4J69X-ray1.89A27-150[»]
4J6AX-ray2.04A27-150[»]
4K7LX-ray1.38A27-41[»]
B47-150[»]
4K7MX-ray1.80A27-150[»]
4L55X-ray1.65A/B27-150[»]
4MXFX-ray2.25A/B27-150[»]
4O36X-ray1.22A27-41[»]
B47-150[»]
4O37X-ray1.40A27-41[»]
B47-150[»]
4OKFX-ray1.54A27-41[»]
B47-150[»]
4OOHX-ray1.89A27-150[»]
4OT4X-ray1.85A/B27-150[»]
4PEQX-ray2.21A/C27-150[»]
4POUX-ray1.75A27-150[»]
4QH3X-ray2.00A/B27-150[»]
4RATX-ray1.50A27-150[»]
4RSDX-ray1.60A27-150[»]
4RSKX-ray2.10A27-150[»]
4RTEX-ray1.95A27-150[»]
4S0QX-ray2.09A/B27-150[»]
4S18X-ray2.27A/B27-150[»]
4SRNX-ray2.00A27-139[»]
B140-150[»]
4WYNX-ray1.80A/B27-150[»]
4WYPX-ray1.50A/B27-150[»]
4WYZX-ray1.45A/B27-150[»]
4YGWX-ray2.18B48-150[»]
b27-41[»]
4ZZ4neutron diffraction1.80A27-150[»]
5D6UX-ray1.52A27-150[»]
5D97neutron diffraction1.80A27-150[»]
5E5EX-ray1.98A/B27-150[»]
5E5FX-ray1.68A/B27-150[»]
5ET4X-ray2.10A/B/C/D27-150[»]
5JLGX-ray1.79A/B27-150[»]
5JMGX-ray1.85A/B27-150[»]
5JMLX-ray2.29A/B27-150[»]
5NA9X-ray2.07A27-150[»]
5OBCX-ray2.07A/B27-150[»]
5OBDX-ray1.98A/B27-150[»]
5OBEX-ray1.82A/B27-150[»]
5RATX-ray1.50A27-150[»]
5RSAX-ray2.00A27-150[»]
6RATX-ray1.50A27-150[»]
6RSAX-ray2.00A27-150[»]
7RATX-ray1.50A27-150[»]
7RSAX-ray1.26A27-150[»]
8RATX-ray1.50A27-150[»]
8RSAX-ray1.80A/B27-150[»]
9RATX-ray1.50A27-150[»]
9RSAX-ray1.80A/B27-150[»]
ProteinModelPortaliP61823.
SMRiP61823.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61823.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni67 – 71Substrate binding5

Sequence similaritiesi

Belongs to the pancreatic ribonuclease family.IKR:

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IYEN. Eukaryota.
ENOG41113F1. LUCA.
GeneTreeiENSGT00900000140967.
HOGENOMiHOG000276883.
HOVERGENiHBG008396.
InParanoidiP61823.
KOiK01168.
OMAiACKNGQT.
OrthoDBiEOG091G15X3.
TreeFamiTF333393.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiView protein in InterPro
IPR001427. RNaseA.
IPR036816. RNaseA-like_dom_sf.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiView protein in Pfam
PF00074. RnaseA. 1 hit.
PRINTSiPR00794. RIBONUCLEASE.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD000535. RNaseA. 1 hit.
SMARTiView protein in SMART
SM00092. RNAse_Pc. 1 hit.
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiView protein in PROSITE
PS00127. RNASE_PANCREATIC. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61823-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALKSLVLLS LLVLVLLLVR VQPSLGKETA AAKFERQHMD SSTSAASSSN
60 70 80 90 100
YCNQMMKSRN LTKDRCKPVN TFVHESLADV QAVCSQKNVA CKNGQTNCYQ
110 120 130 140 150
SYSTMSITDC RETGSSKYPN CAYKTTQANK HIIVACEGNP YVPVHFDASV
Length:150
Mass (Da):16,461
Last modified:June 7, 2004 - v1
Checksum:i74E5DC61E679187D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07283 Genomic DNA. Translation: CAA30263.1.
BC149529 mRNA. Translation: AAI49530.1.
BC149636 mRNA. Translation: AAI49637.1.
S80747 mRNA. Translation: AAB35594.1.
S81740 Genomic DNA. Translation: AAB36134.1.
PIRiS00897. NRBO.
RefSeqiNP_001014408.2. NM_001014386.4.
XP_005211519.1. XM_005211462.2.
UniGeneiBt.4630.

Genome annotation databases

EnsembliENSBTAT00000011586; ENSBTAP00000011586; ENSBTAG00000008793.
GeneIDi282340.
KEGGibta:282340.

Similar proteinsi

Entry informationi

Entry nameiRNAS1_BOVIN
AccessioniPrimary (citable) accession number: P61823
Secondary accession number(s): A6QPW8, P00656, Q9TSF2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: June 7, 2004
Last modified: October 25, 2017
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families