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Protein

Precorrin-2 dehydrogenase

Gene

sirC

Organism
Bacillus megaterium
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the dehydrogenation of precorrin-2 to form sirohydrochlorin which is used as a precursor in both siroheme biosynthesis and in the anaerobic branch of adenosylcobalamin biosynthesis. It is unable to oxidize precorrin-3.2 Publications

Catalytic activityi

Precorrin-2 + NAD+ = sirohydrochlorin + NADH.2 Publications

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sirohydrochlorin from precorrin-2.
Proteins known to be involved in this subpathway in this organism are:
  1. Precorrin-2 dehydrogenase (sirC)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sirohydrochlorin from precorrin-2, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sirohydrochlorin from precorrin-2.
Proteins known to be involved in this subpathway in this organism are:
  1. Precorrin-2 dehydrogenase (CN918_08165), Precorrin-2 dehydrogenase (CJ194_22010), Precorrin-2 dehydrogenase (CN367_05020), Precorrin-2 dehydrogenase (sirC), Precorrin-2 dehydrogenase (BTA37_20270), Precorrin-2 dehydrogenase (SAMN04487776_105375), Precorrin-2 dehydrogenase (CN383_18630)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sirohydrochlorin from precorrin-2, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi20 – 21NADBy similarity2
Nucleotide bindingi41 – 42NADBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processCobalamin biosynthesis, Porphyrin biosynthesis
LigandNAD

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-18855
BRENDAi1.3.1.76 656
UniPathwayiUPA00148; UER00222
UPA00262; UER00222

Names & Taxonomyi

Protein namesi
Recommended name:
Precorrin-2 dehydrogenase (EC:1.3.1.76)
Gene namesi
Name:sirC
OrganismiBacillus megaterium
Taxonomic identifieri1404 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi98N → A: 4-fold decrease in the dehydrogenase activity. 1 Publication1
Mutagenesisi101S → A: 1.4-fold increase in the dehydrogenase activity. 1 Publication1
Mutagenesisi101S → D: 2.8-fold increase in the dehydrogenase activity. 1 Publication1
Mutagenesisi102S → A: 2.3-fold increase in the dehydrogenase activity. 1 Publication1
Mutagenesisi105D → A: 3.2-fold increase in the dehydrogenase activity. 1 Publication1
Mutagenesisi124S → A: 3-fold decrease in the dehydrogenase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000977731 – 202Precorrin-2 dehydrogenaseAdd BLAST202

Interactioni

Subunit structurei

Homodimer.2 Publications

Structurei

Secondary structure

1202
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 6Combined sources4
Beta strandi13 – 16Combined sources4
Helixi20 – 29Combined sources10
Helixi30 – 32Combined sources3
Beta strandi36 – 39Combined sources4
Helixi45 – 52Combined sources8
Beta strandi57 – 59Combined sources3
Helixi65 – 67Combined sources3
Beta strandi68 – 70Combined sources3
Beta strandi72 – 76Combined sources5
Helixi82 – 90Combined sources9
Beta strandi96 – 98Combined sources3
Beta strandi107 – 109Combined sources3
Beta strandi112 – 116Combined sources5
Beta strandi119 – 124Combined sources6
Helixi130 – 143Combined sources14
Helixi147 – 164Combined sources18
Helixi169 – 178Combined sources10
Helixi182 – 186Combined sources5
Helixi188 – 199Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DFZX-ray2.30A/B1-202[»]
ProteinModelPortaliP61818
SMRiP61818
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61818

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108Z73 Bacteria
COG1648 LUCA

Family and domain databases

InterProiView protein in InterPro
IPR028161 Met8
IPR036291 NAD(P)-bd_dom_sf
IPR028281 Sirohaem_synthase_central
IPR006367 Sirohaem_synthase_N
PANTHERiPTHR35330 PTHR35330, 1 hit
PfamiView protein in Pfam
PF14824 Sirohm_synth_M, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR01470 cysG_Nterm, 1 hit

Sequencei

Sequence statusi: Complete.

P61818-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYTVMLDLKG RSVLVVGGGT IATRRIKGFL QEGAAITVVA PTVSAEINEW
60 70 80 90 100
EAKGQLRVKR KKVGEEDLLN VFFIVVATND QAVNKFVKQH IKNDQLVNMA
110 120 130 140 150
SSFSDGNIQI PAQFSRGRLS LAISTDGASP LLTKRIKEDL SSNYDESYTQ
160 170 180 190 200
YTQFLYECRV LIHRLNVSKS RKHELLTEII DDQYRLSLVK QREFLQQIEK

YK
Length:202
Mass (Da):22,970
Last modified:June 7, 2004 - v1
Checksum:i7AA89B591622DEAD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ509159 Genomic DNA Translation: CAD48923.1

Similar proteinsi

Entry informationi

Entry nameiSIRC_BACME
AccessioniPrimary (citable) accession number: P61818
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: March 28, 2018
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure
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Main funding by: National Institutes of Health