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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1

Gene

Dad1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Required for the assembly of both SST3A- and SS3B-containing OST complexes. Required for efficient N-glycosylation. Loss of the DAD1 protein triggers apoptosis.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processApoptosis

Enzyme and pathway databases

UniPathwayiUPA00378

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Short name:
Oligosaccharyl transferase subunit DAD1
Alternative name(s):
Defender against cell death 1
Short name:
DAD-1
Gene namesi
Name:Dad1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 15

Organism-specific databases

RGDi621028 Dad1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 30CytoplasmicSequence analysisAdd BLAST29
Transmembranei31 – 51HelicalSequence analysisAdd BLAST21
Topological domaini52LumenalSequence analysis1
Transmembranei53 – 73HelicalSequence analysisAdd BLAST21
Topological domaini74 – 92CytoplasmicSequence analysisAdd BLAST19
Transmembranei93 – 113HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001240142 – 113Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1Add BLAST112

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP61805
PRIDEiP61805

Expressioni

Gene expression databases

BgeeiENSRNOG00000009090
GenevisibleiP61805 RN

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex. OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes (By similarity).By similarity

Protein-protein interaction databases

IntActiP61805, 2 interactors
MINTiP61805
STRINGi10116.ENSRNOP00000012233

Structurei

3D structure databases

ProteinModelPortaliP61805
SMRiP61805
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DAD/OST2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1746 Eukaryota
ENOG4111Q2I LUCA
GeneTreeiENSGT00390000003324
HOGENOMiHOG000211854
HOVERGENiHBG004452
InParanoidiP61805
KOiK12668
OMAiGVCLRLQ
OrthoDBiEOG091G197A
PhylomeDBiP61805
TreeFamiTF312846

Family and domain databases

InterProiView protein in InterPro
IPR003038 DAD/Ost2
PANTHERiPTHR10705 PTHR10705, 1 hit
PfamiView protein in Pfam
PF02109 DAD, 1 hit
PIRSFiPIRSF005588 DAD, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61805-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASVVSVIS RFLEEYLSST PQRLKLLDAY LLYILLTGAL QFGYCLLVGT
60 70 80 90 100
FPFNSFLSGF ISCVGSFILA VCLRIQINPQ NKADFQGISP ERAFADFLFA
110
STILHLVVMN FVG
Length:113
Mass (Da):12,497
Last modified:January 23, 2007 - v3
Checksum:i481983CADCEB2345
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13336 Genomic DNA Translation: CAA73780.1
BC061530 mRNA Translation: AAH61530.1
RefSeqiNP_620265.1, NM_138910.3
UniGeneiRn.7262

Genome annotation databases

EnsembliENSRNOT00000012233; ENSRNOP00000012233; ENSRNOG00000009090
GeneIDi192275
KEGGirno:192275
UCSCiRGD:621028 rat

Similar proteinsi

Entry informationi

Entry nameiDAD1_RAT
AccessioniPrimary (citable) accession number: P61805
Secondary accession number(s): O08552
, O70364, P46966, P46968, Q96GB7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 102 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health