Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P61804 (DAD1_MOUSE)

Last modified November 3, 2009. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
      Short name=Oligosaccharyl transferase subunit DAD1
    EC=2.4.1.119
Alternative name(s):
    Defender against cell death 1
      Short name=DAD-1
Gene names
Name: Dad1
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMus

Protein attributes

Sequence length113 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER) By similarity. Loss of the DAD1 protein triggers apoptosis.

Catalytic activity

Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.

Subunit structure

Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes By similarity.

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Sequence similarities

Belongs to the DAD/OST2 family.

Ontologies

Keywords
   Biological processApoptosis
   Cellular componentMembrane
   DomainTransmembrane
   Molecular functionTransferase
   PTMAcetylation
Gene Ontology (GO)
   Biological processanti-apoptosis

Inferred from mutant phenotype. Source: MGI

apoptosis

Inferred from electronic annotation. Source: UniProtKB-KW

blastocyst development

Inferred from mutant phenotype. Source: MGI

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiondolichyl-diphosphooligosaccharide-protein glycotransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 113112Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
PRO_0000124011

Regions

Transmembrane31 – 5121 Potential
Transmembrane53 – 7321 Potential

Amino acid modifications

Modified residue21N-acetylserine By similarity

Experimental info

Sequence conflict181S → N in AAC53098. Ref.2
Sequence conflict291A → G in AAA85855. Ref.1
Sequence conflict711V → G Ref.7
Sequence conflict1001A → G in AAA85855. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P61804-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 481983CADCEB2345

FASTA11312,497
        10         20         30         40         50         60 
MSASVVSVIS RFLEEYLSST PQRLKLLDAY LLYILLTGAL QFGYCLLVGT FPFNSFLSGF 

        70         80         90        100        110 
ISCVGSFILA VCLRIQINPQ NKADFQGISP ERAFADFLFA STILHLVVMN FVG 

« Hide

References

« Hide 'large scale' references
[1]"The highly conserved defender against the death 1 (DAD1) gene maps to human chromosome 14q11-q12 and mouse chromosome 14 and has plant and nematode homologs."
Apte S.S., Mattei M.-G., Seldin M.F., Olsen B.R.
FEBS Lett. 363:304-306(1995) [PubMed: 7737422] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"A targeted mutation at the T-cell receptor alpha/delta locus impairs T-cell development and reveals the presence of the nearby antiapoptosis gene Dad1."
Hong N.A., Cado D., Mitchell J., Ortiz B.D., Hsieh S.N., Winoto A.
Mol. Cell. Biol. 17:2151-2157(1997) [PubMed: 9121464] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/Sv.
[3]Wang K., Gan L., Kuo C.L., Hood L.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[4]"Molecular cloning of the murine homologue of DAD-1, an ubiquitously expressed and highly conserved suppressor of apoptosis."
Giegerich G.
Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: BALB/c.
[5]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Kidney, Ovary, Testis and Thymus.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6 and FVB/N.
Tissue: Brain and Colon.
[7]Brewster J., Zachrone K., Hood L., Coffin J.D.
Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-71.
Strain: 129/SvJ.
+Additional computationally mapped references.

Cross-references

Sequence databases

U22107 mRNA. Translation: AAA85855.1.
U81051, U81050 Genomic DNA. Translation: AAC53098.1.
U83628 mRNA. Translation: AAC53359.1.
U84210, U84209 Genomic DNA. Translation: AAB58539.1.
Y13335 Genomic DNA. Translation: CAA73779.1.
AK002446 mRNA. Translation: BAB22107.1.
AK006711 mRNA. Translation: BAB24711.1.
AK030925 mRNA. Translation: BAE20464.1.
AK054480 mRNA. Translation: BAC35796.1.
BC024378 mRNA. Translation: AAH24378.1.
BC053379 mRNA. Translation: AAH53379.1.
BC058116 mRNA. Translation: AAH58116.1.
AF051310 Genomic DNA. Translation: AAC05296.1.
IPIIPI00109082.
PIRI49285.
RefSeqNP_001106829.1.
NP_034145.1.
UniGeneMm.319038

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGP61804.

Genome annotation databases

EnsemblENSMUST00000022781; ENSMUSP00000022781; ENSMUSG00000022174; Mus musculus. [Genome view]
GeneID13135.
KEGGmmu:13135.
UCSCuc007tvm.1. mouse.

Organism-specific databases

CTD13135.
MGIMGI:101912. Dad1.

Phylogenomic databases

HOVERGENP61804.
OMAYCCLVGT.

Enzyme and pathway databases

BRENDA2.4.1.119. 244.

Gene expression databases

ArrayExpressP61804.
BgeeP61804.
CleanExMM_DAD1.
GenevestigatorP61804.
GermOnlineENSMUSG00000022174. Mus musculus.

Family and domain databases

InterProIPR003038. DAD.
[Graphical view]
PANTHERPTHR10705. DAD. 1 hit.
PfamPF02109. DAD. 1 hit.
[Graphical view]
PIRSFPIRSF005588. DAD. 1 hit.
ProtoNetSearch...

Other Resources

NextBio457678.
SOURCESearch...

Entry information

Entry nameDAD1_MOUSE
AccessionPrimary (citable) accession number: P61804
Secondary accession number(s): O08552 expand/collapse secondary AC list , O70364, P46966, P46968, Q3V3W6, Q96GB7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: January 23, 2007
Last modified: November 3, 2009
This is version 60 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents