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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1

Gene

Dad1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Required for the assembly of both SST3A- and SS3B-containing OST complexes. Required for efficient N-glycosylation. Loss of the DAD1 protein triggers apoptosis.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • blastocyst development Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • protein glycosylation Source: UniProtKB
  • protein N-linked glycosylation Source: GO_Central
  • response to drug Source: Ensembl
  • response to nutrient Source: Ensembl

Keywordsi

Biological processApoptosis

Enzyme and pathway databases

UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Short name:
Oligosaccharyl transferase subunit DAD1
Alternative name(s):
Defender against cell death 1
Short name:
DAD-1
Gene namesi
Name:Dad1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:101912. Dad1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 30CytoplasmicSequence analysisAdd BLAST29
Transmembranei31 – 51HelicalSequence analysisAdd BLAST21
Topological domaini52LumenalSequence analysis1
Transmembranei53 – 73HelicalSequence analysisAdd BLAST21
Topological domaini74 – 92CytoplasmicSequence analysisAdd BLAST19
Transmembranei93 – 113HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001240112 – 113Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1Add BLAST112

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP61804.
MaxQBiP61804.
PaxDbiP61804.
PeptideAtlasiP61804.
PRIDEiP61804.
TopDownProteomicsiP61804.

PTM databases

iPTMnetiP61804.
PhosphoSitePlusiP61804.

Expressioni

Gene expression databases

BgeeiENSMUSG00000022174.
CleanExiMM_DAD1.
GenevisibleiP61804. MM.

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex. OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022781.

Structurei

3D structure databases

ProteinModelPortaliP61804.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DAD/OST2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1746. Eukaryota.
ENOG4111Q2I. LUCA.
GeneTreeiENSGT00390000003324.
HOVERGENiHBG004452.
InParanoidiP61804.
KOiK12668.
OMAiGVCLRLQ.
OrthoDBiEOG091G197A.
PhylomeDBiP61804.
TreeFamiTF312846.

Family and domain databases

InterProiView protein in InterPro
IPR003038. DAD/Ost2.
PANTHERiPTHR10705. PTHR10705. 1 hit.
PfamiView protein in Pfam
PF02109. DAD. 1 hit.
PIRSFiPIRSF005588. DAD. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61804-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASVVSVIS RFLEEYLSST PQRLKLLDAY LLYILLTGAL QFGYCLLVGT
60 70 80 90 100
FPFNSFLSGF ISCVGSFILA VCLRIQINPQ NKADFQGISP ERAFADFLFA
110
STILHLVVMN FVG
Length:113
Mass (Da):12,497
Last modified:January 23, 2007 - v3
Checksum:i481983CADCEB2345
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18S → N in AAC53098 (PubMed:9121464).Curated1
Sequence conflicti29A → G in AAA85855 (PubMed:7737422).Curated1
Sequence conflicti71V → G in AAC05296 (Ref. 7) Curated1
Sequence conflicti100A → G in AAA85855 (PubMed:7737422).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22107 mRNA. Translation: AAA85855.1.
U81051, U81050 Genomic DNA. Translation: AAC53098.1.
U83628 mRNA. Translation: AAC53359.1.
U84210, U84209 Genomic DNA. Translation: AAB58539.1.
Y13335 Genomic DNA. Translation: CAA73779.1.
AK002446 mRNA. Translation: BAB22107.1.
AK006711 mRNA. Translation: BAB24711.1.
AK030925 mRNA. Translation: BAE20464.1.
AK054480 mRNA. Translation: BAC35796.1.
BC024378 mRNA. Translation: AAH24378.1.
BC053379 mRNA. Translation: AAH53379.1.
BC058116 mRNA. Translation: AAH58116.1.
AF051310 Genomic DNA. Translation: AAC05296.1.
CCDSiCCDS27084.1.
PIRiI49285.
RefSeqiNP_001106829.1. NM_001113358.1.
NP_034145.1. NM_010015.4.
UniGeneiMm.319038.

Genome annotation databases

EnsembliENSMUST00000022781; ENSMUSP00000022781; ENSMUSG00000022174.
ENSMUST00000128231; ENSMUSP00000122366; ENSMUSG00000022174.
GeneIDi13135.
KEGGimmu:13135.
UCSCiuc007tvm.2. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiDAD1_MOUSE
AccessioniPrimary (citable) accession number: P61804
Secondary accession number(s): O08552
, O70364, P46966, P46968, Q3V3W6, Q96GB7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: January 23, 2007
Last modified: June 7, 2017
This is version 123 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families