P61804 (DAD1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Short name=Oligosaccharyl transferase subunit DAD1
EC=2.4.1.119

Alternative name(s):
Defender against cell death 1
Short name=DAD-1

Gene names

Name:

Dad1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	113 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER) By similarity. Loss of the DAD1 protein triggers apoptosis.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes By similarity.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the DAD/OST2 family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Transferase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	anti-apoptosis Inferred from mutant phenotype. Source: MGI apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW blastocyst development Inferred from mutant phenotype. Source: MGI protein glycosylation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW oligosaccharyltransferase complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 113	112	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1		PRO_0000124011
Regions
	Transmembrane	31 – 51	21	Helical; Potential
	Transmembrane	53 – 73	21	Helical; Potential
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
Experimental info
	Sequence conflict	18	1	S → N in AAC53098. Ref.2
	Sequence conflict	29	1	A → G in AAA85855. Ref.1
	Sequence conflict	71	1	V → G in AAC05296. Ref.7
	Sequence conflict	100	1	A → G in AAA85855. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P61804 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 481983CADCEB2345	FASTA	113	12,497
	10 20 30 40 50 60 MSASVVSVIS RFLEEYLSST PQRLKLLDAY LLYILLTGAL QFGYCLLVGT FPFNSFLSGF 70 80 90 100 110 ISCVGSFILA VCLRIQINPQ NKADFQGISP ERAFADFLFA STILHLVVMN FVG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The highly conserved defender against the death 1 (DAD1) gene maps to human chromosome 14q11-q12 and mouse chromosome 14 and has plant and nematode homologs." Apte S.S., Mattei M.-G., Seldin M.F., Olsen B.R. FEBS Lett. 363:304-306(1995) [PubMed: 7737422] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"A targeted mutation at the T-cell receptor alpha/delta locus impairs T-cell development and reveals the presence of the nearby antiapoptosis gene Dad1." Hong N.A., Cado D., Mitchell J., Ortiz B.D., Hsieh S.N., Winoto A. Mol. Cell. Biol. 17:2151-2157(1997) [PubMed: 9121464] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv.
[3]	Wang K., Gan L., Kuo C.L., Hood L. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[4]	"Molecular cloning of the murine homologue of DAD-1, an ubiquitously expressed and highly conserved suppressor of apoptosis." Giegerich G. Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BALB/c.
[5]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Kidney, Ovary, Testis and Thymus.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6 and FVB/N. Tissue: Brain and Colon.
[7]	Brewster J., Zachrone K., Hood L., Coffin J.D. Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-71. Strain: 129/SvJ.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U22107 mRNA. Translation: AAA85855.1. U81051, U81050 Genomic DNA. Translation: AAC53098.1. U83628 mRNA. Translation: AAC53359.1. U84210, U84209 Genomic DNA. Translation: AAB58539.1. Y13335 Genomic DNA. Translation: CAA73779.1. AK002446 mRNA. Translation: BAB22107.1. AK006711 mRNA. Translation: BAB24711.1. AK030925 mRNA. Translation: BAE20464.1. AK054480 mRNA. Translation: BAC35796.1. BC024378 mRNA. Translation: AAH24378.1. BC053379 mRNA. Translation: AAH53379.1. BC058116 mRNA. Translation: AAH58116.1. AF051310 Genomic DNA. Translation: AAC05296.1.
IPI	IPI00109082.
PIR	I49285.
RefSeq	NP_001106829.1. NM_001113358.1. NP_034145.1. NM_010015.4.
UniGene	Mm.319038.
3D structure databases
ProteinModelPortal	P61804.
ModBase	Search...
Protein-protein interaction databases
STRING	P61804.
PTM databases
PhosphoSite	P61804.
Proteomic databases
PRIDE	P61804.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000022781; ENSMUSP00000022781; ENSMUSG00000022174. ENSMUST00000128231; ENSMUSP00000122366; ENSMUSG00000022174.
GeneID	13135.
KEGG	mmu:13135.
Organism-specific databases
CTD	1603.
MGI	MGI:101912. Dad1.
Phylogenomic databases
eggNOG	roNOG17065.
GeneTree	ENSGT00390000003324.
HOVERGEN	HBG004452.
InParanoid	P61804.
OMA	YCCLVGT.
OrthoDB	EOG4G7C0W.
PhylomeDB	P61804.
Gene expression databases
ArrayExpress	P61804.
Bgee	P61804.
CleanEx	MM_DAD1.
Genevestigator	P61804.
GermOnline	ENSMUSG00000022174. Mus musculus.
Family and domain databases
InterPro	IPR003038. DAD. [Graphical view]
KO	K12668.
PANTHER	PTHR10705. DAD. 1 hit.
Pfam	PF02109. DAD. 1 hit. [Graphical view]
PIRSF	PIRSF005588. DAD. 1 hit.
ProtoNet	Search...
Other
NextBio	457678.
SOURCE	Search...

Entry information

Entry name

DAD1_MOUSE

Accession

Primary (citable) accession number: P61804
Secondary accession number(s): O08552 , O70364, P46966, P46968, Q3V3W6, Q96GB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 80 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program