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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1

Gene

DAD1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis (By similarity).By similarity

Catalytic activityi

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Pathwayi

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Apoptosis

Enzyme and pathway databases

ReactomeiREACT_22426. Asparagine N-linked glycosylation.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 (EC:2.4.99.18)
Short name:
Oligosaccharyl transferase subunit DAD1
Alternative name(s):
Defender against cell death 1
Short name:
DAD-1
Gene namesi
Name:DAD1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:2664. DAD1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 3029CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei31 – 5121HelicalSequence AnalysisAdd
BLAST
Topological domaini52 – 521LumenalSequence Analysis
Transmembranei53 – 7321HelicalSequence AnalysisAdd
BLAST
Topological domaini74 – 9219CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei93 – 11321HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: Reactome
  • extracellular exosome Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: UniProtKB
  • oligosaccharyltransferase complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27136.

Polymorphism and mutation databases

BioMutaiDAD1.
DMDMi48428858.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed3 Publications
Chaini2 – 113112Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1PRO_0000124009Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine2 Publications

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP61803.
PaxDbiP61803.
PRIDEiP61803.

PTM databases

PhosphoSiteiP61803.

Expressioni

Gene expression databases

BgeeiP61803.
CleanExiHS_DAD1.
ExpressionAtlasiP61803. baseline and differential.
GenevestigatoriP61803.

Organism-specific databases

HPAiHPA028882.

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes (By similarity).By similarity

Protein-protein interaction databases

BioGridi107973. 9 interactions.
IntActiP61803. 2 interactions.
STRINGi9606.ENSP00000250498.

Structurei

3D structure databases

ProteinModelPortaliP61803.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DAD/OST2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG246737.
GeneTreeiENSGT00390000003324.
HOVERGENiHBG004452.
InParanoidiP61803.
KOiK12668.
OMAiIDAYMFY.
OrthoDBiEOG7WT43N.
PhylomeDBiP61803.
TreeFamiTF312846.

Family and domain databases

InterProiIPR003038. DAD/Ost2.
[Graphical view]
PANTHERiPTHR10705. PTHR10705. 1 hit.
PfamiPF02109. DAD. 1 hit.
[Graphical view]
PIRSFiPIRSF005588. DAD. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61803-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASVVSVIS RFLEEYLSST PQRLKLLDAY LLYILLTGAL QFGYCLLVGT
60 70 80 90 100
FPFNSFLSGF ISCVGSFILA VCLRIQINPQ NKADFQGISP ERAFADFLFA
110
STILHLVVMN FVG
Length:113
Mass (Da):12,497
Last modified:January 23, 2007 - v3
Checksum:i481983CADCEB2345
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti20 – 201T → I in AAH09798 (PubMed:15489334).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti83 – 831A → T.1 Publication
Corresponds to variant rs5742796 [ dbSNP | Ensembl ].
VAR_018825

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D15057 mRNA. Translation: BAA03650.1.
U84213, U84212 Genomic DNA. Translation: AAB58540.1.
CR407682 mRNA. Translation: CAG28610.1.
CR542204 mRNA. Translation: CAG47001.1.
AY259117 Genomic DNA. Translation: AAO74827.1.
CH471078 Genomic DNA. Translation: EAW66252.1.
CH471078 Genomic DNA. Translation: EAW66253.1.
BC007403 mRNA. Translation: AAH07403.1.
BC009798 mRNA. Translation: AAH09798.1.
CCDSiCCDS9571.1.
PIRiA54437.
RefSeqiNP_001335.1. NM_001344.3.
UniGeneiHs.82890.

Genome annotation databases

EnsembliENST00000250498; ENSP00000250498; ENSG00000129562.
GeneIDi1603.
KEGGihsa:1603.
UCSCiuc001wgl.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D15057 mRNA. Translation: BAA03650.1.
U84213, U84212 Genomic DNA. Translation: AAB58540.1.
CR407682 mRNA. Translation: CAG28610.1.
CR542204 mRNA. Translation: CAG47001.1.
AY259117 Genomic DNA. Translation: AAO74827.1.
CH471078 Genomic DNA. Translation: EAW66252.1.
CH471078 Genomic DNA. Translation: EAW66253.1.
BC007403 mRNA. Translation: AAH07403.1.
BC009798 mRNA. Translation: AAH09798.1.
CCDSiCCDS9571.1.
PIRiA54437.
RefSeqiNP_001335.1. NM_001344.3.
UniGeneiHs.82890.

3D structure databases

ProteinModelPortaliP61803.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107973. 9 interactions.
IntActiP61803. 2 interactions.
STRINGi9606.ENSP00000250498.

PTM databases

PhosphoSiteiP61803.

Polymorphism and mutation databases

BioMutaiDAD1.
DMDMi48428858.

Proteomic databases

MaxQBiP61803.
PaxDbiP61803.
PRIDEiP61803.

Protocols and materials databases

DNASUi1603.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000250498; ENSP00000250498; ENSG00000129562.
GeneIDi1603.
KEGGihsa:1603.
UCSCiuc001wgl.2. human.

Organism-specific databases

CTDi1603.
GeneCardsiGC14M023033.
HGNCiHGNC:2664. DAD1.
HPAiHPA028882.
MIMi600243. gene.
neXtProtiNX_P61803.
PharmGKBiPA27136.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG246737.
GeneTreeiENSGT00390000003324.
HOVERGENiHBG004452.
InParanoidiP61803.
KOiK12668.
OMAiIDAYMFY.
OrthoDBiEOG7WT43N.
PhylomeDBiP61803.
TreeFamiTF312846.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiREACT_22426. Asparagine N-linked glycosylation.

Miscellaneous databases

ChiTaRSiDAD1. human.
GeneWikiiDAD1.
GenomeRNAii1603.
NextBioi6578.
PROiP61803.
SOURCEiSearch...

Gene expression databases

BgeeiP61803.
CleanExiHS_DAD1.
ExpressionAtlasiP61803. baseline and differential.
GenevestigatoriP61803.

Family and domain databases

InterProiIPR003038. DAD/Ost2.
[Graphical view]
PANTHERiPTHR10705. PTHR10705. 1 hit.
PfamiPF02109. DAD. 1 hit.
[Graphical view]
PIRSFiPIRSF005588. DAD. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells."
    Nakashima T., Sekiguchi T., Kuraoka A., Fukushima K., Shibata Y., Komiyama S., Nishimoto T.
    Mol. Cell. Biol. 13:6367-6374(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Wang K., Lee I.Y., Hood L.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. NIEHS SNPs program
    Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT THR-83.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain and Skin.
  8. "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
    Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
    Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-11.
    Tissue: Platelet.
  9. Bienvenut W.V.
    Submitted (JUN-2005) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-23, ACETYLATION AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: B-cell lymphoma.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDAD1_HUMAN
AccessioniPrimary (citable) accession number: P61803
Secondary accession number(s): D3DS25
, O08552, O70364, P46966, P46968, Q6FGA3, Q96GB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: January 23, 2007
Last modified: May 27, 2015
This is version 110 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.