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P61803

- DAD1_HUMAN

UniProt

P61803 - DAD1_HUMAN

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Protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Gene
DAD1
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis By similarity.

Catalytic activityi

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Pathwayi

GO - Molecular functioni

  1. dolichyl-diphosphooligosaccharide-protein glycotransferase activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. blastocyst development Source: Ensembl
  3. cellular protein metabolic process Source: Reactome
  4. negative regulation of apoptotic process Source: ProtInc
  5. post-translational protein modification Source: Reactome
  6. protein N-linked glycosylation via asparagine Source: HGNC
  7. protein glycosylation Source: UniProtKB
  8. response to drug Source: Ensembl
  9. response to nutrient Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Apoptosis

Enzyme and pathway databases

ReactomeiREACT_22426. Asparagine N-linked glycosylation.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 (EC:2.4.99.18)
Short name:
Oligosaccharyl transferase subunit DAD1
Alternative name(s):
Defender against cell death 1
Short name:
DAD-1
Gene namesi
Name:DAD1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 14

Organism-specific databases

HGNCiHGNC:2664. DAD1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 3029Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei31 – 5121Helical; Reviewed prediction
Add
BLAST
Topological domaini52 – 521Lumenal Reviewed prediction
Transmembranei53 – 7321Helical; Reviewed prediction
Add
BLAST
Topological domaini74 – 9219Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei93 – 11321Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: Reactome
  2. extracellular vesicular exosome Source: UniProt
  3. integral component of membrane Source: UniProtKB-KW
  4. oligosaccharyltransferase complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27136.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 113112Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
PRO_0000124009Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine2 Publications

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP61803.
PaxDbiP61803.
PRIDEiP61803.

PTM databases

PhosphoSiteiP61803.

Expressioni

Gene expression databases

ArrayExpressiP61803.
BgeeiP61803.
CleanExiHS_DAD1.
GenevestigatoriP61803.

Organism-specific databases

HPAiHPA028882.

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes By similarity.

Protein-protein interaction databases

BioGridi107973. 6 interactions.
IntActiP61803. 2 interactions.
STRINGi9606.ENSP00000250498.

Structurei

3D structure databases

ProteinModelPortaliP61803.

Family & Domainsi

Sequence similaritiesi

Belongs to the DAD/OST2 family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG246737.
HOVERGENiHBG004452.
InParanoidiP61803.
KOiK12668.
OMAiQSLGKAY.
OrthoDBiEOG7WT43N.
PhylomeDBiP61803.
TreeFamiTF312846.

Family and domain databases

InterProiIPR003038. DAD/Ost2.
[Graphical view]
PANTHERiPTHR10705. PTHR10705. 1 hit.
PfamiPF02109. DAD. 1 hit.
[Graphical view]
PIRSFiPIRSF005588. DAD. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61803-1 [UniParc]FASTAAdd to Basket

« Hide

MSASVVSVIS RFLEEYLSST PQRLKLLDAY LLYILLTGAL QFGYCLLVGT    50
FPFNSFLSGF ISCVGSFILA VCLRIQINPQ NKADFQGISP ERAFADFLFA 100
STILHLVVMN FVG 113
Length:113
Mass (Da):12,497
Last modified:January 23, 2007 - v3
Checksum:i481983CADCEB2345
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti83 – 831A → T.1 Publication
Corresponds to variant rs5742796 [ dbSNP | Ensembl ].
VAR_018825

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti20 – 201T → I in AAH09798. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D15057 mRNA. Translation: BAA03650.1.
U84213, U84212 Genomic DNA. Translation: AAB58540.1.
CR407682 mRNA. Translation: CAG28610.1.
CR542204 mRNA. Translation: CAG47001.1.
AY259117 Genomic DNA. Translation: AAO74827.1.
CH471078 Genomic DNA. Translation: EAW66252.1.
CH471078 Genomic DNA. Translation: EAW66253.1.
BC007403 mRNA. Translation: AAH07403.1.
BC009798 mRNA. Translation: AAH09798.1.
CCDSiCCDS9571.1.
PIRiA54437.
RefSeqiNP_001335.1. NM_001344.3.
UniGeneiHs.82890.

Genome annotation databases

EnsembliENST00000250498; ENSP00000250498; ENSG00000129562.
GeneIDi1603.
KEGGihsa:1603.
UCSCiuc001wgl.2. human.

Polymorphism databases

DMDMi48428858.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D15057 mRNA. Translation: BAA03650.1 .
U84213 , U84212 Genomic DNA. Translation: AAB58540.1 .
CR407682 mRNA. Translation: CAG28610.1 .
CR542204 mRNA. Translation: CAG47001.1 .
AY259117 Genomic DNA. Translation: AAO74827.1 .
CH471078 Genomic DNA. Translation: EAW66252.1 .
CH471078 Genomic DNA. Translation: EAW66253.1 .
BC007403 mRNA. Translation: AAH07403.1 .
BC009798 mRNA. Translation: AAH09798.1 .
CCDSi CCDS9571.1.
PIRi A54437.
RefSeqi NP_001335.1. NM_001344.3.
UniGenei Hs.82890.

3D structure databases

ProteinModelPortali P61803.
ModBasei Search...

Protein-protein interaction databases

BioGridi 107973. 6 interactions.
IntActi P61803. 2 interactions.
STRINGi 9606.ENSP00000250498.

PTM databases

PhosphoSitei P61803.

Polymorphism databases

DMDMi 48428858.

Proteomic databases

MaxQBi P61803.
PaxDbi P61803.
PRIDEi P61803.

Protocols and materials databases

DNASUi 1603.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000250498 ; ENSP00000250498 ; ENSG00000129562 .
GeneIDi 1603.
KEGGi hsa:1603.
UCSCi uc001wgl.2. human.

Organism-specific databases

CTDi 1603.
GeneCardsi GC14M023033.
HGNCi HGNC:2664. DAD1.
HPAi HPA028882.
MIMi 600243. gene.
neXtProti NX_P61803.
PharmGKBi PA27136.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG246737.
HOVERGENi HBG004452.
InParanoidi P61803.
KOi K12668.
OMAi QSLGKAY.
OrthoDBi EOG7WT43N.
PhylomeDBi P61803.
TreeFami TF312846.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_22426. Asparagine N-linked glycosylation.

Miscellaneous databases

ChiTaRSi DAD1. human.
GeneWikii DAD1.
GenomeRNAii 1603.
NextBioi 6578.
PROi P61803.
SOURCEi Search...

Gene expression databases

ArrayExpressi P61803.
Bgeei P61803.
CleanExi HS_DAD1.
Genevestigatori P61803.

Family and domain databases

InterProi IPR003038. DAD/Ost2.
[Graphical view ]
PANTHERi PTHR10705. PTHR10705. 1 hit.
Pfami PF02109. DAD. 1 hit.
[Graphical view ]
PIRSFi PIRSF005588. DAD. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells."
    Nakashima T., Sekiguchi T., Kuraoka A., Fukushima K., Shibata Y., Komiyama S., Nishimoto T.
    Mol. Cell. Biol. 13:6367-6374(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Wang K., Lee I.Y., Hood L.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. NIEHS SNPs program
    Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT THR-83.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain and Skin.
  8. "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
    Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
    Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-11.
    Tissue: Platelet.
  9. Bienvenut W.V.
    Submitted (JUN-2005) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-23, ACETYLATION AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: B-cell lymphoma.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDAD1_HUMAN
AccessioniPrimary (citable) accession number: P61803
Secondary accession number(s): D3DS25
, O08552, O70364, P46966, P46968, Q6FGA3, Q96GB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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