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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1

Gene

DAD1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Required for the assembly of both SST3A- and SS3B-containing OST complexes. Required for efficient N-glycosylation. Loss of the DAD1 protein triggers apoptosis.Curated1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processApoptosis

Enzyme and pathway databases

ReactomeiR-HSA-446203. Asparagine N-linked glycosylation.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Short name:
Oligosaccharyl transferase subunit DAD1
Alternative name(s):
Defender against cell death 1
Short name:
DAD-1
Gene namesi
Name:DAD1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:2664. DAD1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 30CytoplasmicSequence analysisAdd BLAST29
Transmembranei31 – 51HelicalSequence analysisAdd BLAST21
Topological domaini52LumenalSequence analysis1
Transmembranei53 – 73HelicalSequence analysisAdd BLAST21
Topological domaini74 – 92CytoplasmicSequence analysisAdd BLAST19
Transmembranei93 – 113HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: Reactome
  • extracellular exosome Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: UniProtKB
  • oligosaccharyltransferase complex Source: UniProtKB

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi1603.
OpenTargetsiENSG00000129562.
PharmGKBiPA27136.

Polymorphism and mutation databases

BioMutaiDAD1.
DMDMi48428858.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources2 Publications
ChainiPRO_00001240092 – 113Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1Add BLAST112

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP61803.
MaxQBiP61803.
PaxDbiP61803.
PeptideAtlasiP61803.
PRIDEiP61803.
TopDownProteomicsiP61803.

PTM databases

iPTMnetiP61803.
PhosphoSitePlusiP61803.
SwissPalmiP61803.

Expressioni

Gene expression databases

BgeeiENSG00000129562.
CleanExiHS_DAD1.
ExpressionAtlasiP61803. baseline and differential.
GenevisibleiP61803. HS.

Organism-specific databases

HPAiHPA028882.

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex. OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes.Curated1 Publication

Protein-protein interaction databases

BioGridi107973. 20 interactors.
IntActiP61803. 8 interactors.
STRINGi9606.ENSP00000250498.

Structurei

3D structure databases

ProteinModelPortaliP61803.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DAD/OST2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1746. Eukaryota.
ENOG4111Q2I. LUCA.
GeneTreeiENSGT00390000003324.
HOVERGENiHBG004452.
InParanoidiP61803.
KOiK12668.
OMAiGVCLRLQ.
OrthoDBiEOG091G197A.
PhylomeDBiP61803.
TreeFamiTF312846.

Family and domain databases

InterProiView protein in InterPro
IPR003038. DAD/Ost2.
PANTHERiPTHR10705. PTHR10705. 1 hit.
PfamiView protein in Pfam
PF02109. DAD. 1 hit.
PIRSFiPIRSF005588. DAD. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61803-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASVVSVIS RFLEEYLSST PQRLKLLDAY LLYILLTGAL QFGYCLLVGT
60 70 80 90 100
FPFNSFLSGF ISCVGSFILA VCLRIQINPQ NKADFQGISP ERAFADFLFA
110
STILHLVVMN FVG
Length:113
Mass (Da):12,497
Last modified:January 23, 2007 - v3
Checksum:i481983CADCEB2345
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20T → I in AAH09798 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01882583A → T1 PublicationCorresponds to variant dbSNP:rs5742796Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D15057 mRNA. Translation: BAA03650.1.
U84213, U84212 Genomic DNA. Translation: AAB58540.1.
CR407682 mRNA. Translation: CAG28610.1.
CR542204 mRNA. Translation: CAG47001.1.
AY259117 Genomic DNA. Translation: AAO74827.1.
CH471078 Genomic DNA. Translation: EAW66252.1.
CH471078 Genomic DNA. Translation: EAW66253.1.
BC007403 mRNA. Translation: AAH07403.1.
BC009798 mRNA. Translation: AAH09798.1.
CCDSiCCDS9571.1.
PIRiA54437.
RefSeqiNP_001335.1. NM_001344.3.
UniGeneiHs.82890.

Genome annotation databases

EnsembliENST00000250498; ENSP00000250498; ENSG00000129562.
GeneIDi1603.
KEGGihsa:1603.
UCSCiuc001wgl.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiDAD1_HUMAN
AccessioniPrimary (citable) accession number: P61803
Secondary accession number(s): D3DS25
, O08552, O70364, P46966, P46968, Q6FGA3, Q96GB7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: January 23, 2007
Last modified: August 30, 2017
This is version 133 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families