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P61803

- DAD1_HUMAN

UniProt

P61803 - DAD1_HUMAN

Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1

Gene

DAD1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis By similarity.By similarity

    Catalytic activityi

    Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

    Pathwayi

    GO - Molecular functioni

    1. dolichyl-diphosphooligosaccharide-protein glycotransferase activity Source: InterPro

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW
    2. blastocyst development Source: Ensembl
    3. cellular protein metabolic process Source: Reactome
    4. negative regulation of apoptotic process Source: ProtInc
    5. post-translational protein modification Source: Reactome
    6. protein glycosylation Source: UniProtKB
    7. protein N-linked glycosylation via asparagine Source: HGNC
    8. response to drug Source: Ensembl
    9. response to nutrient Source: Ensembl

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Apoptosis

    Enzyme and pathway databases

    ReactomeiREACT_22426. Asparagine N-linked glycosylation.
    UniPathwayiUPA00378.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 (EC:2.4.99.18)
    Short name:
    Oligosaccharyl transferase subunit DAD1
    Alternative name(s):
    Defender against cell death 1
    Short name:
    DAD-1
    Gene namesi
    Name:DAD1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 14

    Organism-specific databases

    HGNCiHGNC:2664. DAD1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: Reactome
    2. extracellular vesicular exosome Source: UniProt
    3. integral component of membrane Source: UniProtKB-KW
    4. membrane Source: UniProtKB
    5. oligosaccharyltransferase complex Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA27136.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed3 Publications
    Chaini2 – 113112Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1PRO_0000124009Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine2 Publications

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP61803.
    PaxDbiP61803.
    PRIDEiP61803.

    PTM databases

    PhosphoSiteiP61803.

    Expressioni

    Gene expression databases

    ArrayExpressiP61803.
    BgeeiP61803.
    CleanExiHS_DAD1.
    GenevestigatoriP61803.

    Organism-specific databases

    HPAiHPA028882.

    Interactioni

    Subunit structurei

    Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes By similarity.By similarity

    Protein-protein interaction databases

    BioGridi107973. 6 interactions.
    IntActiP61803. 2 interactions.
    STRINGi9606.ENSP00000250498.

    Structurei

    3D structure databases

    ProteinModelPortaliP61803.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 3029CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini52 – 521LumenalSequence Analysis
    Topological domaini74 – 9219CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei31 – 5121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei53 – 7321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei93 – 11321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DAD/OST2 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG246737.
    HOVERGENiHBG004452.
    InParanoidiP61803.
    KOiK12668.
    OMAiQSLGKAY.
    OrthoDBiEOG7WT43N.
    PhylomeDBiP61803.
    TreeFamiTF312846.

    Family and domain databases

    InterProiIPR003038. DAD/Ost2.
    [Graphical view]
    PANTHERiPTHR10705. PTHR10705. 1 hit.
    PfamiPF02109. DAD. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005588. DAD. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P61803-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSASVVSVIS RFLEEYLSST PQRLKLLDAY LLYILLTGAL QFGYCLLVGT    50
    FPFNSFLSGF ISCVGSFILA VCLRIQINPQ NKADFQGISP ERAFADFLFA 100
    STILHLVVMN FVG 113
    Length:113
    Mass (Da):12,497
    Last modified:January 23, 2007 - v3
    Checksum:i481983CADCEB2345
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti20 – 201T → I in AAH09798. (PubMed:15489334)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti83 – 831A → T.1 Publication
    Corresponds to variant rs5742796 [ dbSNP | Ensembl ].
    VAR_018825

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D15057 mRNA. Translation: BAA03650.1.
    U84213, U84212 Genomic DNA. Translation: AAB58540.1.
    CR407682 mRNA. Translation: CAG28610.1.
    CR542204 mRNA. Translation: CAG47001.1.
    AY259117 Genomic DNA. Translation: AAO74827.1.
    CH471078 Genomic DNA. Translation: EAW66252.1.
    CH471078 Genomic DNA. Translation: EAW66253.1.
    BC007403 mRNA. Translation: AAH07403.1.
    BC009798 mRNA. Translation: AAH09798.1.
    CCDSiCCDS9571.1.
    PIRiA54437.
    RefSeqiNP_001335.1. NM_001344.3.
    UniGeneiHs.82890.

    Genome annotation databases

    EnsembliENST00000250498; ENSP00000250498; ENSG00000129562.
    GeneIDi1603.
    KEGGihsa:1603.
    UCSCiuc001wgl.2. human.

    Polymorphism databases

    DMDMi48428858.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    NIEHS-SNPs

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D15057 mRNA. Translation: BAA03650.1 .
    U84213 , U84212 Genomic DNA. Translation: AAB58540.1 .
    CR407682 mRNA. Translation: CAG28610.1 .
    CR542204 mRNA. Translation: CAG47001.1 .
    AY259117 Genomic DNA. Translation: AAO74827.1 .
    CH471078 Genomic DNA. Translation: EAW66252.1 .
    CH471078 Genomic DNA. Translation: EAW66253.1 .
    BC007403 mRNA. Translation: AAH07403.1 .
    BC009798 mRNA. Translation: AAH09798.1 .
    CCDSi CCDS9571.1.
    PIRi A54437.
    RefSeqi NP_001335.1. NM_001344.3.
    UniGenei Hs.82890.

    3D structure databases

    ProteinModelPortali P61803.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 107973. 6 interactions.
    IntActi P61803. 2 interactions.
    STRINGi 9606.ENSP00000250498.

    PTM databases

    PhosphoSitei P61803.

    Polymorphism databases

    DMDMi 48428858.

    Proteomic databases

    MaxQBi P61803.
    PaxDbi P61803.
    PRIDEi P61803.

    Protocols and materials databases

    DNASUi 1603.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000250498 ; ENSP00000250498 ; ENSG00000129562 .
    GeneIDi 1603.
    KEGGi hsa:1603.
    UCSCi uc001wgl.2. human.

    Organism-specific databases

    CTDi 1603.
    GeneCardsi GC14M023033.
    HGNCi HGNC:2664. DAD1.
    HPAi HPA028882.
    MIMi 600243. gene.
    neXtProti NX_P61803.
    PharmGKBi PA27136.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG246737.
    HOVERGENi HBG004452.
    InParanoidi P61803.
    KOi K12668.
    OMAi QSLGKAY.
    OrthoDBi EOG7WT43N.
    PhylomeDBi P61803.
    TreeFami TF312846.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_22426. Asparagine N-linked glycosylation.

    Miscellaneous databases

    ChiTaRSi DAD1. human.
    GeneWikii DAD1.
    GenomeRNAii 1603.
    NextBioi 6578.
    PROi P61803.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P61803.
    Bgeei P61803.
    CleanExi HS_DAD1.
    Genevestigatori P61803.

    Family and domain databases

    InterProi IPR003038. DAD/Ost2.
    [Graphical view ]
    PANTHERi PTHR10705. PTHR10705. 1 hit.
    Pfami PF02109. DAD. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005588. DAD. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells."
      Nakashima T., Sekiguchi T., Kuraoka A., Fukushima K., Shibata Y., Komiyama S., Nishimoto T.
      Mol. Cell. Biol. 13:6367-6374(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Wang K., Lee I.Y., Hood L.
      Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. NIEHS SNPs program
      Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT THR-83.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain and Skin.
    8. "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
      Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
      Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-11.
      Tissue: Platelet.
    9. Bienvenut W.V.
      Submitted (JUN-2005) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 2-23, ACETYLATION AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: B-cell lymphoma.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiDAD1_HUMAN
    AccessioniPrimary (citable) accession number: P61803
    Secondary accession number(s): D3DS25
    , O08552, O70364, P46966, P46968, Q6FGA3, Q96GB7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 105 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 14
      Human chromosome 14: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3