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Protein

Lysophosphatidic acid receptor 1

Gene

Lpar1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for lysophosphatidic acid (LPA). Plays a role in the reorganization of the actin cytoskeleton, cell migration, differentiation and proliferation, and thereby contributes to the responses to tissue damage and infectious agents. Activates downstream signaling cascades via the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Signaling inhibits adenylyl cyclase activity and decreases cellular cAMP levels. Signaling triggers an increase of cytoplasmic Ca2+ levels. Activates RALA; this leads to the activation of phospholipase C (PLC) and the formation of inositol 1,4,5-trisphosphate. Signaling mediates activation of down-stream MAP kinases. Contributes to the regulation of cell shape. Promotes Rho-dependent reorganization of the actin cytoskeleton in neuronal cells and neurite retraction. Promotes the activation of Rho and the formation of actin stress fibers. Promotes formation of lamellipodia at the leading edge of migrating cells via activation of RAC1. Through its function as lysophosphatidic acid receptor, plays a role in chemotaxis and cell migration, including responses to injury and wounding. Plays a role in triggering inflammation in response to bacterial lipopolysaccharide (LPS) via its interaction with CD14. Promotes cell proliferation in response to lysophosphatidic acid. Required for normal skeleton development. May play a role in osteoblast differentiation. Required for normal brain development. Required for normal proliferation, survival and maturation of newly formed neurons in the adult dentate gyrus. Plays a role in pain perception and in the initiation of neuropathic pain.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei39 – 391Lysophosphatidic acidBy similarity
Binding sitei210 – 2101Lysophosphatidic acidBy similarity

GO - Molecular functioni

  • G-protein alpha-subunit binding Source: RGD
  • G-protein coupled receptor activity Source: RGD
  • lysophosphatidic acid binding Source: Ensembl
  • lysophosphatidic acid receptor activity Source: UniProtKB
  • phospholipid binding Source: RGD

GO - Biological processi

  • activation of MAPK activity Source: Ensembl
  • activation of phospholipase C activity Source: UniProtKB
  • adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Source: UniProtKB
  • bleb assembly Source: Ensembl
  • brain development Source: RGD
  • cell chemotaxis Source: Ensembl
  • cellular response to 1-oleoyl-sn-glycerol 3-phosphate Source: RGD
  • cellular response to oxygen levels Source: RGD
  • cerebellum development Source: RGD
  • corpus callosum development Source: RGD
  • G-protein coupled receptor signaling pathway Source: RGD
  • myelination Source: RGD
  • negative regulation of cAMP biosynthetic process Source: RGD
  • negative regulation of neuron projection development Source: UniProtKB
  • neurogenesis Source: RGD
  • oligodendrocyte development Source: RGD
  • optic nerve development Source: RGD
  • positive regulation of apoptotic process Source: RGD
  • positive regulation of cell death Source: RGD
  • positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway Source: RGD
  • positive regulation of dendritic spine development Source: RGD
  • positive regulation of I-kappaB kinase/NF-kappaB signaling Source: Ensembl
  • positive regulation of MAPK cascade Source: UniProtKB
  • positive regulation of Rho protein signal transduction Source: UniProtKB
  • positive regulation of smooth muscle cell chemotaxis Source: RGD
  • positive regulation of stress fiber assembly Source: UniProtKB
  • regulation of cell shape Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-RNO-416476. G alpha (q) signalling events.
R-RNO-418594. G alpha (i) signalling events.
R-RNO-419408. Lysosphingolipid and LPA receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysophosphatidic acid receptor 1
Short name:
LPA receptor 1
Short name:
LPA-1
Alternative name(s):
Lysophosphatidic acid receptor Edg-2
Gene namesi
Name:Lpar1
Synonyms:Edg21 Publication, Gpcr91, Lpa1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi620563. Lpar1.

Subcellular locationi

  • Cell surface By similarity
  • Cell membrane By similarity; Multi-pass membrane protein By similarity
  • Endosome By similarity

  • Note: Prior to LPA treatment found predominantly at the cell surface. Internalized after LPA treatment. Colocalizes with RALA in endocytic vesicles after LPA treatment.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5050ExtracellularBy similarityAdd
BLAST
Transmembranei51 – 7525Helical; Name=1By similarityAdd
BLAST
Topological domaini76 – 838CytoplasmicBy similarity
Transmembranei84 – 10724Helical; Name=2By similarityAdd
BLAST
Topological domaini108 – 12114ExtracellularBy similarityAdd
BLAST
Transmembranei122 – 14423Helical; Name=3By similarityAdd
BLAST
Topological domaini145 – 16319CytoplasmicBy similarityAdd
BLAST
Transmembranei164 – 18421Helical; Name=4By similarityAdd
BLAST
Topological domaini185 – 20420ExtracellularBy similarityAdd
BLAST
Transmembranei205 – 22521Helical; Name=5By similarityAdd
BLAST
Topological domaini226 – 25530CytoplasmicBy similarityAdd
BLAST
Transmembranei256 – 28025Helical; Name=6By similarityAdd
BLAST
Topological domaini281 – 29414ExtracellularBy similarityAdd
BLAST
Transmembranei295 – 31521Helical; Name=7By similarityAdd
BLAST
Topological domaini316 – 36449CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • cell surface Source: UniProtKB
  • cytoplasm Source: RGD
  • cytosol Source: Ensembl
  • dendritic shaft Source: RGD
  • dendritic spine Source: RGD
  • endocytic vesicle Source: Ensembl
  • endosome Source: UniProtKB-SubCell
  • integral component of plasma membrane Source: UniProtKB
  • neuronal cell body Source: RGD
  • neuron projection Source: RGD
  • plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL4595.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 364364Lysophosphatidic acid receptor 1PRO_0000069419Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi24 ↔ 190By similarity
Glycosylationi27 – 271N-linked (GlcNAc...)Sequence analysis
Glycosylationi35 – 351N-linked (GlcNAc...)Sequence analysis
Disulfide bondi188 ↔ 195By similarity
Disulfide bondi284 ↔ 287By similarity
Modified residuei341 – 3411PhosphoserineBy similarity
Modified residuei351 – 3511PhosphothreonineBy similarity

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP61794.

PTM databases

PhosphoSiteiP61794.

Expressioni

Tissue specificityi

Detected in brain corpus callosum, medulla oblongata, cerebellum and sciatic nerve. Detected in heart.1 Publication

Gene expression databases

GenevisibleiP61794. RN.

Interactioni

Subunit structurei

Interacts with RALA and ADRBK1 (By similarity). Interacts with GNAQ and GNA13. Interacts with CD14; the interaction is enhanced by exposure to bacterial lipopolysaccharide (LPS) (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043652.

Chemistry

BindingDBiP61794.

Structurei

3D structure databases

ProteinModelPortaliP61794.
SMRiP61794. Positions 23-232, 248-326.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni124 – 1296Lysophosphatidic acid bindingBy similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118804.
HOGENOMiHOG000233501.
HOVERGENiHBG103071.
InParanoidiP61794.
KOiK04289.
OMAiCQRSENT.
OrthoDBiEOG7HTHH1.
PhylomeDBiP61794.
TreeFamiTF330052.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR004065. LPA_rcpt.
IPR002277. LPA_rcpt_EDG2.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01148. EDG2RECEPTOR.
PR00237. GPCRRHODOPSN.
PR01527. LPARECEPTOR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P61794-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAASTSSPV ISQPQFTAMN EQQCFYNESI AFFYNRSGKY LATEWNTVSK
60 70 80 90 100
LVMGLGITVC VFIMLANLLV MVAIYVNRRF HFPIYYLMAN LAAADFFAGL
110 120 130 140 150
AYFYLMFNTG PNTRRLTVST WLLRQGLIDT SLTASVANLL AIAIERHITV
160 170 180 190 200
FRMQLHTRMS NRRVVVVIVV IWTMAIVMGA IPSVGWNCIC DIDHCSNMAP
210 220 230 240 250
LYSDSYLVFW AIFNLVTFVV MVVLYAHIFG YVRQRTMRMS RHSSGPRRNR
260 270 280 290 300
DTMMSLLKTV VIVLGAFIVC WTPGLVLLLL DVCCPQCDVL AYEKFFLLLA
310 320 330 340 350
EFNSAMNPII YSYRDKEMSA TFRQILCCQR NENPNGPTEG SDRSASSLNH
360
TILAGVHSND HSVV
Length:364
Mass (Da):41,119
Last modified:June 7, 2004 - v1
Checksum:iB0FA6265AA6688B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF014418 mRNA. Translation: AAB86381.1.
AF090347 mRNA. Translation: AAG24469.1.
BC089227 mRNA. Translation: AAH89227.1.
RefSeqiNP_446388.1. NM_053936.3.
XP_006238257.1. XM_006238195.2.
XP_006238259.1. XM_006238197.2.
XP_006238262.1. XM_006238200.2.
XP_006238263.1. XM_006238201.2.
XP_008761954.1. XM_008763732.1.
UniGeneiRn.11200.

Genome annotation databases

EnsembliENSRNOT00000044348; ENSRNOP00000043652; ENSRNOG00000013656.
GeneIDi116744.
KEGGirno:116744.
UCSCiRGD:620563. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF014418 mRNA. Translation: AAB86381.1.
AF090347 mRNA. Translation: AAG24469.1.
BC089227 mRNA. Translation: AAH89227.1.
RefSeqiNP_446388.1. NM_053936.3.
XP_006238257.1. XM_006238195.2.
XP_006238259.1. XM_006238197.2.
XP_006238262.1. XM_006238200.2.
XP_006238263.1. XM_006238201.2.
XP_008761954.1. XM_008763732.1.
UniGeneiRn.11200.

3D structure databases

ProteinModelPortaliP61794.
SMRiP61794. Positions 23-232, 248-326.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043652.

Chemistry

BindingDBiP61794.
ChEMBLiCHEMBL4595.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP61794.

Proteomic databases

PaxDbiP61794.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000044348; ENSRNOP00000043652; ENSRNOG00000013656.
GeneIDi116744.
KEGGirno:116744.
UCSCiRGD:620563. rat.

Organism-specific databases

CTDi1902.
RGDi620563. Lpar1.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118804.
HOGENOMiHOG000233501.
HOVERGENiHBG103071.
InParanoidiP61794.
KOiK04289.
OMAiCQRSENT.
OrthoDBiEOG7HTHH1.
PhylomeDBiP61794.
TreeFamiTF330052.

Enzyme and pathway databases

ReactomeiR-RNO-416476. G alpha (q) signalling events.
R-RNO-418594. G alpha (i) signalling events.
R-RNO-419408. Lysosphingolipid and LPA receptors.

Miscellaneous databases

NextBioi286803.
PROiP61794.

Gene expression databases

GenevisibleiP61794. RN.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR004065. LPA_rcpt.
IPR002277. LPA_rcpt_EDG2.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01148. EDG2RECEPTOR.
PR00237. GPCRRHODOPSN.
PR01527. LPARECEPTOR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A rat G protein-coupled receptor selectively expressed in myelin-forming cells."
    Allard J., Barron S., Diaz J., Lubetzki C., Zalc B., Schwartz J.-C., Sokoloff P.
    Eur. J. Neurosci. 10:1045-1053(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Olfactory bulb.
  2. "Identification and characterization of novel G-protein coupled receptors expressed in regenerating peripheral nerve."
    Carroll S.L., Miller M.L., Benedict-Hamilton H.M.
    Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiLPAR1_RAT
AccessioniPrimary (citable) accession number: P61794
Secondary accession number(s): O88584
, P56487, P70420, Q5FWS2, Q61130
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: January 20, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.