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Protein

Syntaxin-binding protein 1

Gene

Stxbp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions.

GO - Molecular functioni

  • identical protein binding Source: BHF-UCL
  • poly(A) RNA binding Source: Ensembl
  • protein domain specific binding Source: RGD
  • protein kinase binding Source: RGD
  • protein N-terminus binding Source: RGD
  • SNARE binding Source: RGD
  • syntaxin-1 binding Source: RGD
  • syntaxin binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-181430. Norepinephrine Neurotransmitter Release Cycle.
R-RNO-210500. Glutamate Neurotransmitter Release Cycle.
R-RNO-264642. Acetylcholine Neurotransmitter Release Cycle.
R-RNO-888590. GABA synthesis, release, reuptake and degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Syntaxin-binding protein 1
Alternative name(s):
N-Sec1
Protein unc-18 homolog 1
Short name:
Unc18-1
Protein unc-18 homolog A
Short name:
Unc-18A
p67
rbSec1
Gene namesi
Name:Stxbp1
Synonyms:Unc18a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi3785. Stxbp1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: BHF-UCL
  • cytosol Source: RGD
  • extracellular exosome Source: Ensembl
  • mitochondrion Source: Ensembl
  • myelin sheath Source: Ensembl
  • nucleoplasm Source: Ensembl
  • plasma membrane Source: RGD
  • platelet alpha granule Source: Ensembl
  • protein complex Source: RGD
  • terminal bouton Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 594594Syntaxin-binding protein 1PRO_0000206279Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei476 – 4761PhosphoserineCombined sources
Modified residuei509 – 5091PhosphoserineCombined sources
Modified residuei511 – 5111PhosphoserineBy similarity
Modified residuei516 – 5161PhosphoserineBy similarity
Modified residuei593 – 5931PhosphoserineCombined sources
Isoform 2 (identifier: P61765-2)
Modified residuei594 – 5941PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP61765.
PRIDEiP61765.

2D gel databases

World-2DPAGE0004:P61765.

PTM databases

iPTMnetiP61765.
PhosphoSiteiP61765.

Expressioni

Tissue specificityi

Brain and spinal cord. Highly enriched in axons.

Developmental stagei

Faint levels are detectable at embryonic day 14, with levels rising at later embryonic ages and peaking at postnatal day 7.

Gene expression databases

BgeeiENSRNOG00000015420.
GenevisibleiP61765. RN.

Interactioni

Subunit structurei

Binds SYTL4 and STX1A.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Apba1O354305EBI-1029097,EBI-704760
Apba2O354313EBI-1029097,EBI-2028211
Stx1aP3285116EBI-1029097,EBI-539720

GO - Molecular functioni

  • identical protein binding Source: BHF-UCL
  • protein domain specific binding Source: RGD
  • protein kinase binding Source: RGD
  • protein N-terminus binding Source: RGD
  • SNARE binding Source: RGD
  • syntaxin-1 binding Source: RGD
  • syntaxin binding Source: RGD

Protein-protein interaction databases

BioGridi247589. 12 interactions.
DIPiDIP-766N.
IntActiP61765. 7 interactions.
STRINGi10116.ENSRNOP00000021189.

Structurei

Secondary structure

1
594
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 1712Combined sources
Turni18 – 214Combined sources
Beta strandi29 – 335Combined sources
Helixi35 – 428Combined sources
Helixi47 – 515Combined sources
Turni52 – 543Combined sources
Beta strandi55 – 606Combined sources
Beta strandi71 – 777Combined sources
Helixi81 – 899Combined sources
Turni90 – 923Combined sources
Helixi94 – 963Combined sources
Beta strandi98 – 1003Combined sources
Beta strandi102 – 1087Combined sources
Helixi112 – 1198Combined sources
Helixi122 – 1265Combined sources
Beta strandi127 – 1326Combined sources
Beta strandi137 – 1415Combined sources
Beta strandi144 – 1463Combined sources
Helixi152 – 1576Combined sources
Helixi159 – 1613Combined sources
Helixi166 – 18318Combined sources
Beta strandi188 – 1914Combined sources
Helixi197 – 21519Combined sources
Turni217 – 2204Combined sources
Helixi225 – 2273Combined sources
Beta strandi229 – 2346Combined sources
Helixi235 – 2373Combined sources
Turni241 – 2433Combined sources
Helixi249 – 2568Combined sources
Beta strandi263 – 28018Combined sources
Helixi286 – 2916Combined sources
Helixi296 – 31318Combined sources
Helixi330 – 3334Combined sources
Helixi335 – 3373Combined sources
Helixi338 – 35821Combined sources
Helixi361 – 37414Combined sources
Helixi387 – 3959Combined sources
Helixi401 – 41515Combined sources
Helixi420 – 43011Combined sources
Helixi434 – 4429Combined sources
Helixi443 – 4464Combined sources
Turni455 – 4573Combined sources
Helixi474 – 4763Combined sources
Helixi481 – 49010Combined sources
Turni496 – 4983Combined sources
Beta strandi501 – 5033Combined sources
Beta strandi535 – 5428Combined sources
Helixi547 – 56014Combined sources
Beta strandi564 – 57310Combined sources
Helixi575 – 5839Combined sources
Turni584 – 5863Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3C98X-ray2.60A1-594[»]
3PUJX-ray3.31A/B1-594[»]
4JEHX-ray2.50A1-594[»]
4JEUX-ray3.20A3-592[»]
ProteinModelPortaliP61765.
SMRiP61765. Positions 4-592.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61765.

Family & Domainsi

Sequence similaritiesi

Belongs to the STXBP/unc-18/SEC1 family.Curated

Phylogenomic databases

eggNOGiKOG1300. Eukaryota.
COG5158. LUCA.
GeneTreeiENSGT00390000005206.
HOGENOMiHOG000232146.
HOVERGENiHBG052710.
InParanoidiP61765.
KOiK15292.
OMAiFILNTIR.
OrthoDBiEOG091G0WUI.
PhylomeDBiP61765.
TreeFamiTF313242.

Family and domain databases

Gene3Di3.40.50.1910. 2 hits.
InterProiIPR027482. Sec-1-like_dom2.
IPR001619. Sec1-like.
[Graphical view]
PANTHERiPTHR11679. PTHR11679. 1 hit.
PfamiPF00995. Sec1. 1 hit.
[Graphical view]
PIRSFiPIRSF005715. VPS45_Sec1. 1 hit.
SUPFAMiSSF56815. SSF56815. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P61765-1) [UniParc]FASTAAdd to basket
Also known as: A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPIGLKAVV GEKIMHDVIK KVKKKGEWKV LVVDQLSMRM LSSCCKMTDI
60 70 80 90 100
MTEGITIVED INKRREPLPS LEAVYLITPS EKSVHSLISD FKDPPTAKYR
110 120 130 140 150
AAHVFFTDSC PDALFNELVK SRAAKVIKTL TEINIAFLPY ESQVYSLDSA
160 170 180 190 200
DSFQSFYSPH KAQMKNPILE RLAEQIATLC ATLKEYPAVR YRGEYKDNAL
210 220 230 240 250
LAQLIQDKLD AYKADDPTMG EGPDKARSQL LILDRGFDPS SPVLHELTFQ
260 270 280 290 300
AMSYDLLPIE NDVYKYETSG IGEARVKEVL LDEDDDLWIA LRHKHIAEVS
310 320 330 340 350
QEVTRSLKDF SSSKRMNTGE KTTMRDLSQM LKKMPQYQKE LSKYSTHLHL
360 370 380 390 400
AEDCMKHYQG TVDKLCRVEQ DLAMGTDAEG EKIKDPMRAI VPILLDANVS
410 420 430 440 450
TYDKIRIILL YIFLKNGITE ENLNKLIQHA QIPPEDSEII TNMAHLGVPI
460 470 480 490 500
VTDSTLRRRS KPERKERISE QTYQLSRWTP IIKDIMEDTI EDKLDTKHYP
510 520 530 540 550
YISTRSSASF STTAVSARYG HWHKNKAPGE YRSGPRLIIF ILGGVSLNEM
560 570 580 590
RCAYEVTQAN GKWEVLIGST HILTPQKLLD TLKKLNKTDE EISS
Length:594
Mass (Da):67,569
Last modified:June 7, 2004 - v1
Checksum:i2DD0715F875CE0F3
GO
Isoform 2 (identifier: P61765-2) [UniParc] [UniParc]FASTAAdd to basket
Also known as: BE, HUNC18b

The sequence of this isoform differs from the canonical sequence as follows:
     576-594: QKLLDTLKKLNKTDEEISS → TKFLMDLRHPDFRESSRVSFEDQAPTME

Show »
Length:603
Mass (Da):68,736
Checksum:i73D8337768259493
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei576 – 59419QKLLD…EEISS → TKFLMDLRHPDFRESSRVSF EDQAPTME in isoform 2. 1 PublicationVSP_010497Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26087 mRNA. Translation: AAB41113.1.
L26264 mRNA. Translation: AAA19246.1.
U06069 mRNA. Translation: AAA17987.1.
U21116 mRNA. Translation: AAA96350.1.
BC088850 mRNA. Translation: AAH88850.1.
PIRiA53455.
S39345.
RefSeqiNP_037170.1. NM_013038.3. [P61765-1]
XP_006234001.1. XM_006233939.2. [P61765-2]
UniGeneiRn.80843.

Genome annotation databases

EnsembliENSRNOT00000021178; ENSRNOP00000021178; ENSRNOG00000015420. [P61765-1]
ENSRNOT00000021189; ENSRNOP00000021189; ENSRNOG00000015420. [P61765-2]
GeneIDi25558.
KEGGirno:25558.
UCSCiRGD:3785. rat.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26087 mRNA. Translation: AAB41113.1.
L26264 mRNA. Translation: AAA19246.1.
U06069 mRNA. Translation: AAA17987.1.
U21116 mRNA. Translation: AAA96350.1.
BC088850 mRNA. Translation: AAH88850.1.
PIRiA53455.
S39345.
RefSeqiNP_037170.1. NM_013038.3. [P61765-1]
XP_006234001.1. XM_006233939.2. [P61765-2]
UniGeneiRn.80843.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3C98X-ray2.60A1-594[»]
3PUJX-ray3.31A/B1-594[»]
4JEHX-ray2.50A1-594[»]
4JEUX-ray3.20A3-592[»]
ProteinModelPortaliP61765.
SMRiP61765. Positions 4-592.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247589. 12 interactions.
DIPiDIP-766N.
IntActiP61765. 7 interactions.
STRINGi10116.ENSRNOP00000021189.

PTM databases

iPTMnetiP61765.
PhosphoSiteiP61765.

2D gel databases

World-2DPAGE0004:P61765.

Proteomic databases

PaxDbiP61765.
PRIDEiP61765.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000021178; ENSRNOP00000021178; ENSRNOG00000015420. [P61765-1]
ENSRNOT00000021189; ENSRNOP00000021189; ENSRNOG00000015420. [P61765-2]
GeneIDi25558.
KEGGirno:25558.
UCSCiRGD:3785. rat.

Organism-specific databases

CTDi6812.
RGDi3785. Stxbp1.

Phylogenomic databases

eggNOGiKOG1300. Eukaryota.
COG5158. LUCA.
GeneTreeiENSGT00390000005206.
HOGENOMiHOG000232146.
HOVERGENiHBG052710.
InParanoidiP61765.
KOiK15292.
OMAiFILNTIR.
OrthoDBiEOG091G0WUI.
PhylomeDBiP61765.
TreeFamiTF313242.

Enzyme and pathway databases

ReactomeiR-RNO-181430. Norepinephrine Neurotransmitter Release Cycle.
R-RNO-210500. Glutamate Neurotransmitter Release Cycle.
R-RNO-264642. Acetylcholine Neurotransmitter Release Cycle.
R-RNO-888590. GABA synthesis, release, reuptake and degradation.

Miscellaneous databases

EvolutionaryTraceiP61765.
PROiP61765.

Gene expression databases

BgeeiENSRNOG00000015420.
GenevisibleiP61765. RN.

Family and domain databases

Gene3Di3.40.50.1910. 2 hits.
InterProiIPR027482. Sec-1-like_dom2.
IPR001619. Sec1-like.
[Graphical view]
PANTHERiPTHR11679. PTHR11679. 1 hit.
PfamiPF00995. Sec1. 1 hit.
[Graphical view]
PIRSFiPIRSF005715. VPS45_Sec1. 1 hit.
SUPFAMiSSF56815. SSF56815. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSTXB1_RAT
AccessioniPrimary (citable) accession number: P61765
Secondary accession number(s): Q28208
, Q62759, Q64320, Q96TG8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: September 7, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.