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Protein

Syntaxin-binding protein 1

Gene

Stxbp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions.

GO - Molecular functioni

  • identical protein binding Source: BHF-UCL
  • poly(A) RNA binding Source: Ensembl
  • protein domain specific binding Source: RGD
  • protein kinase binding Source: RGD
  • protein N-terminus binding Source: RGD
  • SNARE binding Source: RGD
  • syntaxin-1 binding Source: RGD
  • syntaxin binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-181430. Norepinephrine Neurotransmitter Release Cycle.
R-RNO-210500. Glutamate Neurotransmitter Release Cycle.
R-RNO-264642. Acetylcholine Neurotransmitter Release Cycle.
R-RNO-6794361. Interactions of neurexins and neuroligins at synapses.
R-RNO-888590. GABA synthesis, release, reuptake and degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Syntaxin-binding protein 1
Alternative name(s):
N-Sec1
Protein unc-18 homolog 1
Short name:
Unc18-1
Protein unc-18 homolog A
Short name:
Unc-18A
p67
rbSec1
Gene namesi
Name:Stxbp1
Synonyms:Unc18a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi3785. Stxbp1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: BHF-UCL
  • cytosol Source: RGD
  • extracellular exosome Source: Ensembl
  • mitochondrion Source: Ensembl
  • myelin sheath Source: Ensembl
  • nucleoplasm Source: Ensembl
  • plasma membrane Source: RGD
  • platelet alpha granule Source: Ensembl
  • protein complex Source: RGD
  • terminal bouton Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002062791 – 594Syntaxin-binding protein 1Add BLAST594

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei476PhosphoserineCombined sources1
Modified residuei509PhosphoserineCombined sources1
Modified residuei511PhosphoserineBy similarity1
Modified residuei516PhosphoserineBy similarity1
Modified residuei593PhosphoserineCombined sources1
Isoform 2 (identifier: P61765-2)
Modified residuei594PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP61765.
PRIDEiP61765.

2D gel databases

World-2DPAGE0004:P61765.

PTM databases

iPTMnetiP61765.
PhosphoSitePlusiP61765.

Expressioni

Tissue specificityi

Brain and spinal cord. Highly enriched in axons.

Developmental stagei

Faint levels are detectable at embryonic day 14, with levels rising at later embryonic ages and peaking at postnatal day 7.

Gene expression databases

BgeeiENSRNOG00000015420.
GenevisibleiP61765. RN.

Interactioni

Subunit structurei

Binds SYTL4 and STX1A.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Apba1O354305EBI-1029097,EBI-704760
Apba2O354313EBI-1029097,EBI-2028211
Stx1aP3285116EBI-1029097,EBI-539720

GO - Molecular functioni

  • identical protein binding Source: BHF-UCL
  • protein domain specific binding Source: RGD
  • protein kinase binding Source: RGD
  • protein N-terminus binding Source: RGD
  • SNARE binding Source: RGD
  • syntaxin-1 binding Source: RGD
  • syntaxin binding Source: RGD

Protein-protein interaction databases

BioGridi247589. 12 interactors.
DIPiDIP-766N.
IntActiP61765. 7 interactors.
STRINGi10116.ENSRNOP00000021189.

Structurei

Secondary structure

1594
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 17Combined sources12
Turni18 – 21Combined sources4
Beta strandi29 – 33Combined sources5
Helixi35 – 42Combined sources8
Helixi47 – 51Combined sources5
Turni52 – 54Combined sources3
Beta strandi55 – 60Combined sources6
Beta strandi71 – 77Combined sources7
Helixi81 – 89Combined sources9
Turni90 – 92Combined sources3
Helixi94 – 96Combined sources3
Beta strandi98 – 100Combined sources3
Beta strandi102 – 108Combined sources7
Helixi112 – 119Combined sources8
Helixi122 – 126Combined sources5
Beta strandi127 – 132Combined sources6
Beta strandi137 – 141Combined sources5
Beta strandi144 – 146Combined sources3
Helixi152 – 157Combined sources6
Helixi159 – 161Combined sources3
Helixi166 – 183Combined sources18
Beta strandi188 – 191Combined sources4
Helixi197 – 215Combined sources19
Turni217 – 220Combined sources4
Helixi225 – 227Combined sources3
Beta strandi229 – 234Combined sources6
Helixi235 – 237Combined sources3
Turni241 – 243Combined sources3
Helixi249 – 256Combined sources8
Beta strandi263 – 280Combined sources18
Helixi286 – 291Combined sources6
Helixi296 – 313Combined sources18
Helixi330 – 333Combined sources4
Helixi335 – 337Combined sources3
Helixi338 – 358Combined sources21
Helixi361 – 374Combined sources14
Helixi387 – 395Combined sources9
Helixi401 – 415Combined sources15
Helixi420 – 430Combined sources11
Helixi434 – 442Combined sources9
Helixi443 – 446Combined sources4
Turni455 – 457Combined sources3
Helixi474 – 476Combined sources3
Helixi481 – 490Combined sources10
Turni496 – 498Combined sources3
Beta strandi501 – 503Combined sources3
Beta strandi535 – 542Combined sources8
Helixi547 – 560Combined sources14
Beta strandi564 – 573Combined sources10
Helixi575 – 583Combined sources9
Turni584 – 586Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3C98X-ray2.60A1-594[»]
3PUJX-ray3.31A/B1-594[»]
4JEHX-ray2.50A1-594[»]
4JEUX-ray3.20A3-592[»]
ProteinModelPortaliP61765.
SMRiP61765.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61765.

Family & Domainsi

Sequence similaritiesi

Belongs to the STXBP/unc-18/SEC1 family.Curated

Phylogenomic databases

eggNOGiKOG1300. Eukaryota.
COG5158. LUCA.
GeneTreeiENSGT00390000005206.
HOGENOMiHOG000232146.
HOVERGENiHBG052710.
InParanoidiP61765.
KOiK15292.
OMAiFILNTIR.
OrthoDBiEOG091G0WUI.
PhylomeDBiP61765.
TreeFamiTF313242.

Family and domain databases

Gene3Di3.40.50.1910. 2 hits.
InterProiIPR027482. Sec-1-like_dom2.
IPR001619. Sec1-like.
[Graphical view]
PANTHERiPTHR11679. PTHR11679. 1 hit.
PfamiPF00995. Sec1. 1 hit.
[Graphical view]
PIRSFiPIRSF005715. VPS45_Sec1. 1 hit.
SUPFAMiSSF56815. SSF56815. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P61765-1) [UniParc]FASTAAdd to basket
Also known as: A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPIGLKAVV GEKIMHDVIK KVKKKGEWKV LVVDQLSMRM LSSCCKMTDI
60 70 80 90 100
MTEGITIVED INKRREPLPS LEAVYLITPS EKSVHSLISD FKDPPTAKYR
110 120 130 140 150
AAHVFFTDSC PDALFNELVK SRAAKVIKTL TEINIAFLPY ESQVYSLDSA
160 170 180 190 200
DSFQSFYSPH KAQMKNPILE RLAEQIATLC ATLKEYPAVR YRGEYKDNAL
210 220 230 240 250
LAQLIQDKLD AYKADDPTMG EGPDKARSQL LILDRGFDPS SPVLHELTFQ
260 270 280 290 300
AMSYDLLPIE NDVYKYETSG IGEARVKEVL LDEDDDLWIA LRHKHIAEVS
310 320 330 340 350
QEVTRSLKDF SSSKRMNTGE KTTMRDLSQM LKKMPQYQKE LSKYSTHLHL
360 370 380 390 400
AEDCMKHYQG TVDKLCRVEQ DLAMGTDAEG EKIKDPMRAI VPILLDANVS
410 420 430 440 450
TYDKIRIILL YIFLKNGITE ENLNKLIQHA QIPPEDSEII TNMAHLGVPI
460 470 480 490 500
VTDSTLRRRS KPERKERISE QTYQLSRWTP IIKDIMEDTI EDKLDTKHYP
510 520 530 540 550
YISTRSSASF STTAVSARYG HWHKNKAPGE YRSGPRLIIF ILGGVSLNEM
560 570 580 590
RCAYEVTQAN GKWEVLIGST HILTPQKLLD TLKKLNKTDE EISS
Length:594
Mass (Da):67,569
Last modified:June 7, 2004 - v1
Checksum:i2DD0715F875CE0F3
GO
Isoform 2 (identifier: P61765-2) [UniParc] [UniParc]FASTAAdd to basket
Also known as: BE, HUNC18b

The sequence of this isoform differs from the canonical sequence as follows:
     576-594: QKLLDTLKKLNKTDEEISS → TKFLMDLRHPDFRESSRVSFEDQAPTME

Show »
Length:603
Mass (Da):68,736
Checksum:i73D8337768259493
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010497576 – 594QKLLD…EEISS → TKFLMDLRHPDFRESSRVSF EDQAPTME in isoform 2. 1 PublicationAdd BLAST19

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26087 mRNA. Translation: AAB41113.1.
L26264 mRNA. Translation: AAA19246.1.
U06069 mRNA. Translation: AAA17987.1.
U21116 mRNA. Translation: AAA96350.1.
BC088850 mRNA. Translation: AAH88850.1.
PIRiA53455.
S39345.
RefSeqiNP_037170.1. NM_013038.3. [P61765-1]
XP_006234001.1. XM_006233939.3. [P61765-2]
UniGeneiRn.80843.

Genome annotation databases

EnsembliENSRNOT00000021178; ENSRNOP00000021178; ENSRNOG00000015420. [P61765-1]
ENSRNOT00000021189; ENSRNOP00000021189; ENSRNOG00000015420. [P61765-2]
GeneIDi25558.
KEGGirno:25558.
UCSCiRGD:3785. rat.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26087 mRNA. Translation: AAB41113.1.
L26264 mRNA. Translation: AAA19246.1.
U06069 mRNA. Translation: AAA17987.1.
U21116 mRNA. Translation: AAA96350.1.
BC088850 mRNA. Translation: AAH88850.1.
PIRiA53455.
S39345.
RefSeqiNP_037170.1. NM_013038.3. [P61765-1]
XP_006234001.1. XM_006233939.3. [P61765-2]
UniGeneiRn.80843.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3C98X-ray2.60A1-594[»]
3PUJX-ray3.31A/B1-594[»]
4JEHX-ray2.50A1-594[»]
4JEUX-ray3.20A3-592[»]
ProteinModelPortaliP61765.
SMRiP61765.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247589. 12 interactors.
DIPiDIP-766N.
IntActiP61765. 7 interactors.
STRINGi10116.ENSRNOP00000021189.

PTM databases

iPTMnetiP61765.
PhosphoSitePlusiP61765.

2D gel databases

World-2DPAGE0004:P61765.

Proteomic databases

PaxDbiP61765.
PRIDEiP61765.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000021178; ENSRNOP00000021178; ENSRNOG00000015420. [P61765-1]
ENSRNOT00000021189; ENSRNOP00000021189; ENSRNOG00000015420. [P61765-2]
GeneIDi25558.
KEGGirno:25558.
UCSCiRGD:3785. rat.

Organism-specific databases

CTDi6812.
RGDi3785. Stxbp1.

Phylogenomic databases

eggNOGiKOG1300. Eukaryota.
COG5158. LUCA.
GeneTreeiENSGT00390000005206.
HOGENOMiHOG000232146.
HOVERGENiHBG052710.
InParanoidiP61765.
KOiK15292.
OMAiFILNTIR.
OrthoDBiEOG091G0WUI.
PhylomeDBiP61765.
TreeFamiTF313242.

Enzyme and pathway databases

ReactomeiR-RNO-181430. Norepinephrine Neurotransmitter Release Cycle.
R-RNO-210500. Glutamate Neurotransmitter Release Cycle.
R-RNO-264642. Acetylcholine Neurotransmitter Release Cycle.
R-RNO-6794361. Interactions of neurexins and neuroligins at synapses.
R-RNO-888590. GABA synthesis, release, reuptake and degradation.

Miscellaneous databases

EvolutionaryTraceiP61765.
PROiP61765.

Gene expression databases

BgeeiENSRNOG00000015420.
GenevisibleiP61765. RN.

Family and domain databases

Gene3Di3.40.50.1910. 2 hits.
InterProiIPR027482. Sec-1-like_dom2.
IPR001619. Sec1-like.
[Graphical view]
PANTHERiPTHR11679. PTHR11679. 1 hit.
PfamiPF00995. Sec1. 1 hit.
[Graphical view]
PIRSFiPIRSF005715. VPS45_Sec1. 1 hit.
SUPFAMiSSF56815. SSF56815. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSTXB1_RAT
AccessioniPrimary (citable) accession number: P61765
Secondary accession number(s): Q28208
, Q62759, Q64320, Q96TG8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: November 2, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.