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Protein

Prefoldin subunit 3

Gene

VBP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Enzyme and pathway databases

ReactomeiR-HSA-389957. Prefoldin mediated transfer of substrate to CCT/TriC.

Names & Taxonomyi

Protein namesi
Recommended name:
Prefoldin subunit 3
Alternative name(s):
HIBBJ46
Von Hippel-Lindau-binding protein 1
Short name:
VBP-1
Short name:
VHL-binding protein 1
Gene namesi
Name:VBP1
Synonyms:PFDN3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:12662. VBP1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37285.

Polymorphism and mutation databases

BioMutaiVBP1.
DMDMi125987848.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 197196Prefoldin subunit 3PRO_0000153652Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources
Modified residuei59 – 591N6-acetyllysineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP61758.
MaxQBiP61758.
PaxDbiP61758.
PRIDEiP61758.
TopDownProteomicsiP61758.

2D gel databases

OGPiP61758.

PTM databases

iPTMnetiP61758.
PhosphoSiteiP61758.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiP61758.
CleanExiHS_VBP1.
ExpressionAtlasiP61758. baseline and differential.
GenevisibleiP61758. HS.

Organism-specific databases

HPAiCAB006260.
HPA023230.

Interactioni

Subunit structurei

Heterohexamer of two PFD-alpha type and four PFD-beta type subunits. Binds to the C-terminal part of VHL.

Binary interactionsi

WithEntry#Exp.IntActNotes
ORFQ9Q2G43EBI-357430,EBI-6248094From a different organism.
PFDN4Q9NQP43EBI-357430,EBI-357021
PNMA1Q8ND903EBI-357430,EBI-302345
UBL7Q96S823EBI-357430,EBI-348604

GO - Molecular functioni

Protein-protein interaction databases

BioGridi113254. 70 interactions.
DIPiDIP-46479N.
IntActiP61758. 18 interactions.
MINTiMINT-1148501.
STRINGi9606.ENSP00000286428.

Structurei

3D structure databases

ProteinModelPortaliP61758.
SMRiP61758. Positions 47-178.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the prefoldin subunit alpha family.Curated

Phylogenomic databases

eggNOGiKOG3313. Eukaryota.
ENOG410XRZ8. LUCA.
HOVERGENiHBG053576.
InParanoidiP61758.
PhylomeDBiP61758.
TreeFamiTF313706.

Family and domain databases

Gene3Di1.10.287.370. 1 hit.
InterProiIPR016655. PFD3.
IPR009053. Prefoldin.
IPR004127. Prefoldin_subunit_alpha.
[Graphical view]
PANTHERiPTHR12409. PTHR12409. 1 hit.
PfamiPF02996. Prefoldin. 1 hit.
[Graphical view]
PIRSFiPIRSF016396. Prefoldin_subunit_3. 1 hit.
SUPFAMiSSF46579. SSF46579. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61758-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAVKDSCGK GEMATGNGRR LHLGIPEAVF VEDVDSFMKQ PGNETADTVL
60 70 80 90 100
KKLDEQYQKY KFMELNLAQK KRRLKGQIPE IKQTLEILKY MQKKKESTNS
110 120 130 140 150
METRFLLADN LYCKASVPPT DKMCLWLGAN VMLEYDIDEA QALLEKNLST
160 170 180 190
ATKNLDSLEE DLDFLRDQFT TTEVNMARVY NWDVKRRNKD DSTKNKA
Length:197
Mass (Da):22,658
Last modified:February 6, 2007 - v3
Checksum:i973BBDE2B3903E39
GO

Sequence cautioni

The sequence AAC23907.1 differs from that shown. Reason: Erroneous initiation. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti123 – 1231M → V.6 Publications
Corresponds to variant rs572013 [ dbSNP | Ensembl ].
VAR_023371

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U96759 mRNA. Translation: AAC23907.1. Different initiation.
Y17394 mRNA. Translation: CAA76761.1.
AK313420 mRNA. Translation: BAG36212.1.
BT019604 mRNA. Translation: AAV38411.1.
AL356738 Genomic DNA. Translation: CAI41469.1.
BC046094 mRNA. Translation: AAH46094.1.
U56833 mRNA. Translation: AAC50617.1.
RefSeqiNP_001290472.1. NM_001303543.1.
NP_001290474.1. NM_001303545.1.
NP_003363.1. NM_003372.6.
UniGeneiHs.436803.

Genome annotation databases

EnsembliENST00000286428; ENSP00000286428; ENSG00000155959.
GeneIDi7411.
KEGGihsa:7411.
UCSCiuc004fnc.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U96759 mRNA. Translation: AAC23907.1. Different initiation.
Y17394 mRNA. Translation: CAA76761.1.
AK313420 mRNA. Translation: BAG36212.1.
BT019604 mRNA. Translation: AAV38411.1.
AL356738 Genomic DNA. Translation: CAI41469.1.
BC046094 mRNA. Translation: AAH46094.1.
U56833 mRNA. Translation: AAC50617.1.
RefSeqiNP_001290472.1. NM_001303543.1.
NP_001290474.1. NM_001303545.1.
NP_003363.1. NM_003372.6.
UniGeneiHs.436803.

3D structure databases

ProteinModelPortaliP61758.
SMRiP61758. Positions 47-178.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113254. 70 interactions.
DIPiDIP-46479N.
IntActiP61758. 18 interactions.
MINTiMINT-1148501.
STRINGi9606.ENSP00000286428.

PTM databases

iPTMnetiP61758.
PhosphoSiteiP61758.

Polymorphism and mutation databases

BioMutaiVBP1.
DMDMi125987848.

2D gel databases

OGPiP61758.

Proteomic databases

EPDiP61758.
MaxQBiP61758.
PaxDbiP61758.
PRIDEiP61758.
TopDownProteomicsiP61758.

Protocols and materials databases

DNASUi7411.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000286428; ENSP00000286428; ENSG00000155959.
GeneIDi7411.
KEGGihsa:7411.
UCSCiuc004fnc.4. human.

Organism-specific databases

CTDi7411.
GeneCardsiVBP1.
HGNCiHGNC:12662. VBP1.
HPAiCAB006260.
HPA023230.
MIMi300133. gene.
neXtProtiNX_P61758.
PharmGKBiPA37285.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3313. Eukaryota.
ENOG410XRZ8. LUCA.
HOVERGENiHBG053576.
InParanoidiP61758.
PhylomeDBiP61758.
TreeFamiTF313706.

Enzyme and pathway databases

ReactomeiR-HSA-389957. Prefoldin mediated transfer of substrate to CCT/TriC.

Miscellaneous databases

ChiTaRSiVBP1. human.
GenomeRNAii7411.
NextBioi29016.
PROiP61758.
SOURCEiSearch...

Gene expression databases

BgeeiP61758.
CleanExiHS_VBP1.
ExpressionAtlasiP61758. baseline and differential.
GenevisibleiP61758. HS.

Family and domain databases

Gene3Di1.10.287.370. 1 hit.
InterProiIPR016655. PFD3.
IPR009053. Prefoldin.
IPR004127. Prefoldin_subunit_alpha.
[Graphical view]
PANTHERiPTHR12409. PTHR12409. 1 hit.
PfamiPF02996. Prefoldin. 1 hit.
[Graphical view]
PIRSFiPIRSF016396. Prefoldin_subunit_3. 1 hit.
SUPFAMiSSF46579. SSF46579. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the gene (VBP1) and transcript for the von Hippel-Lindau binding protein and isolation of the highly conserved murine homologue."
    Brinke A., Green P.M., Giannelli F.
    Genomics 45:105-112(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT VAL-123.
    Tissue: Brain.
  2. "Prefoldin, a chaperone that delivers unfolded proteins to cytosolic chaperonin."
    Vainberg I.E., Lewis S.A., Rommelaere H., Ampe C., Vandekerckhove J., Klein H.L., Cowan N.J.
    Cell 93:863-873(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT VAL-123, FUNCTION.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-123.
    Tissue: Cerebellum.
  4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-123.
  5. "The DNA sequence of the human X chromosome."
    Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.
    , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
    Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-123.
    Tissue: Lung.
  7. "Identification of a novel protein (VBP-1) binding to the von Hippel-Lindau (VHL) tumor suppressor gene product."
    Tsuchiya H., Iseda T., Hino O.
    Cancer Res. 56:2881-2885(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 32-197, VARIANT VAL-123.
  8. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
    Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
    Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-59, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPFD3_HUMAN
AccessioniPrimary (citable) accession number: P61758
Secondary accession number(s): B2R8L5
, O55228, Q15765, Q5JT81, Q86X96
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: February 6, 2007
Last modified: May 11, 2016
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.