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Protein

ADP-ribosylation factor 4

Gene

Arf4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi24 – 318GTPBy similarity
Nucleotide bindingi67 – 715GTPBy similarity
Nucleotide bindingi126 – 1294GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-5620916. VxPx cargo-targeting to cilium.
R-RNO-6807878. COPI-mediated anterograde transport.
R-RNO-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 4
Gene namesi
Name:Arf4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 16

Organism-specific databases

RGDi621275. Arf4.

Subcellular locationi

  • Golgi apparatus
  • Membrane By similarity; Lipid-anchor By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 180179ADP-ribosylation factor 4PRO_0000207394Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiP61751.
PRIDEiP61751.

PTM databases

iPTMnetiP61751.
PhosphoSiteiP61751.
SwissPalmiP61751.

Expressioni

Gene expression databases

GenevisibleiP61751. RN.

Interactioni

Protein-protein interaction databases

IntActiP61751. 1 interaction.
MINTiMINT-4088235.
STRINGi10116.ENSRNOP00000017692.

Structurei

3D structure databases

ProteinModelPortaliP61751.
SMRiP61751. Positions 4-178.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0070. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP61751.
KOiK07939.
OMAiWASSEIF.
OrthoDBiEOG77WWDV.
PhylomeDBiP61751.
TreeFamiTF300808.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61751-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLTISSLFS RLFGKKQMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG
60 70 80 90 100
FNVETVEYKN ICFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERI
110 120 130 140 150
QEGAAVLQKM LLEDELQDAV LLLFANKQDL PNAMAISEMT DKLGLQSLRN
160 170 180
RTWYVQATCA TQGTGLYEGL DWLSNELSKR
Length:180
Mass (Da):20,397
Last modified:January 23, 2007 - v2
Checksum:i09112917D8CE15D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12383 mRNA. Translation: AAA40688.1.
BC063167 mRNA. Translation: AAH63167.1.
PIRiI55371.
RefSeqiNP_077065.1. NM_024151.1.
UniGeneiRn.35935.

Genome annotation databases

EnsembliENSRNOT00000017692; ENSRNOP00000017692; ENSRNOG00000012623.
GeneIDi79120.
KEGGirno:79120.
UCSCiRGD:621275. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12383 mRNA. Translation: AAA40688.1.
BC063167 mRNA. Translation: AAH63167.1.
PIRiI55371.
RefSeqiNP_077065.1. NM_024151.1.
UniGeneiRn.35935.

3D structure databases

ProteinModelPortaliP61751.
SMRiP61751. Positions 4-178.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP61751. 1 interaction.
MINTiMINT-4088235.
STRINGi10116.ENSRNOP00000017692.

PTM databases

iPTMnetiP61751.
PhosphoSiteiP61751.
SwissPalmiP61751.

Proteomic databases

PaxDbiP61751.
PRIDEiP61751.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000017692; ENSRNOP00000017692; ENSRNOG00000012623.
GeneIDi79120.
KEGGirno:79120.
UCSCiRGD:621275. rat.

Organism-specific databases

CTDi378.
RGDi621275. Arf4.

Phylogenomic databases

eggNOGiKOG0070. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP61751.
KOiK07939.
OMAiWASSEIF.
OrthoDBiEOG77WWDV.
PhylomeDBiP61751.
TreeFamiTF300808.

Enzyme and pathway databases

ReactomeiR-RNO-5620916. VxPx cargo-targeting to cilium.
R-RNO-6807878. COPI-mediated anterograde transport.
R-RNO-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Miscellaneous databases

PROiP61751.

Gene expression databases

GenevisibleiP61751. RN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Regulation of ADP-ribosylation factor (ARF) expression. Cross-species conservation of the developmental and tissue-specific alternative polyadenylation of ARF 4 mRNA."
    Mishima K., Price S.R., Nightingale M.S., Kousvelari E., Moss J., Vaughan M.
    J. Biol. Chem. 267:24109-24116(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Testis.
  2. "Interspecies relationships among ADP-ribosylation factors (ARFs): evidence of evolutionary pressure to maintain individual identities."
    Price S.R., Nightingale M.S., Tsuchiya M., Moss J., Vaughan M.
    Mol. Cell. Biochem. 159:15-23(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pituitary.

Entry informationi

Entry nameiARF4_RAT
AccessioniPrimary (citable) accession number: P61751
Secondary accession number(s): P36403
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.