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Protein

ADP-ribosylation factor 4

Gene

Arf4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi24 – 31GTPBy similarity8
Nucleotide bindingi67 – 71GTPBy similarity5
Nucleotide bindingi126 – 129GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-5620916. VxPx cargo-targeting to cilium.
R-MMU-6807878. COPI-mediated anterograde transport.
R-MMU-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 4
Gene namesi
Name:Arf4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:99433. Arf4.

Subcellular locationi

  • Golgi apparatus
  • Membrane By similarity; Lipid-anchor By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • dendritic spine Source: MGI
  • extracellular exosome Source: MGI
  • extracellular matrix Source: MGI
  • Golgi apparatus Source: MGI
  • membrane Source: MGI
  • plasma membrane Source: MGI
  • ruffle membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002073922 – 180ADP-ribosylation factor 4Add BLAST179

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Modified residuei147PhosphoserineBy similarity1

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

EPDiP61750.
PaxDbiP61750.
PeptideAtlasiP61750.
PRIDEiP61750.

PTM databases

iPTMnetiP61750.
PhosphoSitePlusiP61750.
SwissPalmiP61750.

Expressioni

Gene expression databases

BgeeiENSMUSG00000021877.
CleanExiMM_ARF4.
ExpressionAtlasiP61750. baseline and differential.
GenevisibleiP61750. MM.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Cadps2Q8BYR52EBI-7569554,EBI-7569313

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198187. 5 interactors.
IntActiP61750. 4 interactors.
MINTiMINT-1866425.
STRINGi10090.ENSMUSP00000022429.

Structurei

3D structure databases

ProteinModelPortaliP61750.
SMRiP61750.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0070. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP61750.
KOiK07939.
OMAiWASSEIF.
OrthoDBiEOG091G0S7S.
PhylomeDBiP61750.
TreeFamiTF300808.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61750-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLTISSLFS RLFGKKQMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG
60 70 80 90 100
FNVETVEYKN ICFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERI
110 120 130 140 150
QEGAAVLQKM LLEDELQDAV LLLFANKQDL PNAMAISEMT DKLGLQSLRN
160 170 180
RTWYVQATCA TQGTGLYEGL DWLSNELSKR
Length:180
Mass (Da):20,397
Last modified:January 23, 2007 - v2
Checksum:i09112917D8CE15D6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti108Q → E in BAB29041 (PubMed:16141072).Curated1
Sequence conflicti176E → R in BAB29041 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87901 mRNA. Translation: BAA13493.1.
AK013892 mRNA. Translation: BAB29041.1.
AK081686 mRNA. Translation: BAC38292.1.
AK153011 mRNA. Translation: BAE31650.1.
AK168793 mRNA. Translation: BAE40626.1.
CCDSiCCDS26881.1.
PIRiJC4948.
RefSeqiNP_031505.1. NM_007479.3.
UniGeneiMm.297768.

Genome annotation databases

EnsembliENSMUST00000022429; ENSMUSP00000022429; ENSMUSG00000021877.
GeneIDi11843.
KEGGimmu:11843.
UCSCiuc007sta.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87901 mRNA. Translation: BAA13493.1.
AK013892 mRNA. Translation: BAB29041.1.
AK081686 mRNA. Translation: BAC38292.1.
AK153011 mRNA. Translation: BAE31650.1.
AK168793 mRNA. Translation: BAE40626.1.
CCDSiCCDS26881.1.
PIRiJC4948.
RefSeqiNP_031505.1. NM_007479.3.
UniGeneiMm.297768.

3D structure databases

ProteinModelPortaliP61750.
SMRiP61750.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198187. 5 interactors.
IntActiP61750. 4 interactors.
MINTiMINT-1866425.
STRINGi10090.ENSMUSP00000022429.

PTM databases

iPTMnetiP61750.
PhosphoSitePlusiP61750.
SwissPalmiP61750.

Proteomic databases

EPDiP61750.
PaxDbiP61750.
PeptideAtlasiP61750.
PRIDEiP61750.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022429; ENSMUSP00000022429; ENSMUSG00000021877.
GeneIDi11843.
KEGGimmu:11843.
UCSCiuc007sta.1. mouse.

Organism-specific databases

CTDi378.
MGIiMGI:99433. Arf4.

Phylogenomic databases

eggNOGiKOG0070. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP61750.
KOiK07939.
OMAiWASSEIF.
OrthoDBiEOG091G0S7S.
PhylomeDBiP61750.
TreeFamiTF300808.

Enzyme and pathway databases

ReactomeiR-MMU-5620916. VxPx cargo-targeting to cilium.
R-MMU-6807878. COPI-mediated anterograde transport.
R-MMU-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Miscellaneous databases

PROiP61750.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021877.
CleanExiMM_ARF4.
ExpressionAtlasiP61750. baseline and differential.
GenevisibleiP61750. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARF4_MOUSE
AccessioniPrimary (citable) accession number: P61750
Secondary accession number(s): P36403, Q3TGC2, Q9CXX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.