P61727 (RISB2_RHOPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6,7-dimethyl-8-ribityllumazine synthase 2
Short name=DMRL synthase 2
Short name=Lumazine synthase 2
EC=2.5.1.9

Alternative name(s):
Riboflavin synthase 2 beta chain

Gene names

Name:	ribH2
Ordered Locus Names:	RPA4363

Organism

Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009) [Complete proteome] [HAMAP]

Taxonomic identifier

258594 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes the condensation of 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate yielding 6,7-dimethyl-8-lumazine By similarity. HAMAP MF_00178
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine. HAMAP MF_00178
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2. HAMAP MF_00178
Sequence similarities	Belongs to the DMRL synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	riboflavin synthase complex Inferred from electronic annotation. Source: InterPro
Molecular function	riboflavin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 181	181	6,7-dimethyl-8-ribityllumazine synthase 2 HAMAP MF_00178		PRO_0000134799

Sequences

	Sequence		Length	Mass (Da)
	P61727 [UniParc]. Last modified June 7, 2004. Version 1. Checksum: 6BA86E729CA49528	FASTA	181	19,760
	10 20 30 40 50 60 MSLPMTETVT DPAETAPPTA ERSYPRFAKP QRVAFVQSSW HRDVVAGCWE SFVREIVERG 70 80 90 100 110 120 IVASQVDLFE VPGSFEIPLH VQMLAKTRRY TAIVAAGLVV DDGLTGFVAQ TVIQALMDVQ 130 140 150 160 170 180 LKTEVPVFSA VATPHSFERG SPNTEHFRQH FATKGADVAH ACADTLLSLE RLRGQVAAGI V

References

[1]

"Complete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris." Larimer F.W., Chain P., Hauser L., Lamerdin J.E., Malfatti S., Do L., Land M.L., Pelletier D.A., Beatty J.T., Lang A.S., Tabita F.R., Gibson J.L., Hanson T.E., Bobst C., Torres y Torres J.L., Peres C., Harrison F.H., Gibson J., Harwood C.S. Nat. Biotechnol. 22:55-61(2004) [PubMed: 14704707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-98 / CGA009.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX572607 Genomic DNA. Translation: CAE29804.1.
RefSeq	NP_949699.1. NC_005296.1.
3D structure databases
ProteinModelPortal	P61727.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2690998.
GenomeReviews	Gene locus RPA4363 in contig BX571963_GR.
KEGG	rpa:RPA4363.
NMPDR	fig|258594.1.peg.4342.
PATRIC	23293327. VBIRhoPal84835_4577.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG311126.
OMA	RRYTAIV.
ProtClustDB	PRK12419.
Enzyme and pathway databases
BioCyc	RPAL258594:RPA4363-MONOMER.
Family and domain databases
HAMAP	MF_00178. Lumazine_synth. [Tree]
InterPro	IPR002180. DMRL_synthase. [Graphical view]
Gene3D	G3DSA:3.40.50.960. DMRL_synthase. 1 hit.
KO	K00794.
PANTHER	PTHR21058. DMRL_synthase. 1 hit.
Pfam	PF00885. DMRL_synthase. 1 hit. [Graphical view]
SUPFAM	SSF52121. DMRL_synthase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RISB2_RHOPA

Accession

Primary (citable) accession number: P61727

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	June 7, 2004
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program