Reviewed,
UniProtKB/Swiss-Prot P61714 (RISB_ECOLI)
Last modified
November 3, 2009.
Version 55.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: 6,7-dimethyl-8-ribityllumazine synthase Short name=DMRL synthase Short name=Lumazine synthase EC=2.5.1.9 Alternative name(s): Riboflavin synthase beta chain | ||||||
| Gene names |
| ||||||
| Organism | Escherichia coli (strain K12) [Complete proteome] [HAMAP] | ||||||
| Taxonomic identifier | 83333 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 156 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes the condensation of 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate yielding 6,7-dimethyl-8-lumazine. HAMAP MF_00178 |
| Catalytic activity | 2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine. HAMAP MF_00178 |
| Pathway | Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil and riboflavin from 6,7-dimethyl-8-(1-D-ribityl)lumazine: step 1/1. HAMAP MF_00178 |
| Subunit structure | Oligomer of 60 beta subunits forming an icosahedral capsid. The core of the capsid does not contain alpha subunits. HAMAP MF_00178 |
| Sequence similarities | Belongs to the DMRL synthase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Riboflavin biosynthesis |
| Molecular function | Transferase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | riboflavin biosynthetic process Inferred from electronic annotation. Source: HAMAP |
| Cellular component | riboflavin synthase complex Inferred from electronic annotation. Source: InterPro |
| Molecular function | riboflavin synthase activity Inferred from electronic annotation. Source: HAMAP |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||
Molecule processing | |||||||
|---|---|---|---|---|---|---|---|
| Chain | 1 – 156 | 156 | 6,7-dimethyl-8-ribityllumazine synthase HAMAP MF_00178 | PRO_0000134754 | |||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Insertional disruption of the nusB (ssyB) gene leads to cold-sensitive growth of Escherichia coli and suppression of the secY24 mutation." Taura T., Ueguchi C., Shiba K., Ito K. Mol. Gen. Genet. 234:429-432(1992) [PubMed: 1406588] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12. |
| [2] | "Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [3] | "The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [4] | "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [5] | "Biosynthesis of riboflavin. Lumazine synthase of Escherichia coli." Moertl S., Fischer M., Richter G., Tack J., Weinkauf S., Bacher A. J. Biol. Chem. 271:33201-33207(1996) [PubMed: 8969176] [Abstract] Cited for: CHARACTERIZATION. |
Cross-references
Sequence databases | |
|---|---|
| X64395 Genomic DNA. Translation: CAA45736.1. U82664 Genomic DNA. Translation: AAB40171.1. U00096 Genomic DNA. Translation: AAC73518.1. AP009048 Genomic DNA. Translation: BAE76195.1. | |
| PIR | S26202. |
| RefSeq | AP_001065.1. NP_414949.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1RVV based on UniProtKB P11998. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P61714. |
2-D gel databases | |
| SWISS-2DPAGE | P61714. |
Genome annotation databases | |
| GeneID | 946453. |
| GenomeReviews | Gene locus JW0405 in contig AP009048_GR. Gene locus b0415 in contig U00096_GR. |
| KEGG | ecj:JW0405. eco:b0415. |
Organism-specific databases | |
| EchoBASE | EB1298. |
| EcoGene | EG11322. ribH. |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | P61714. |
| OMA | LTTDTMQ. |
Enzyme and pathway databases | |
| BioCyc | EcoCyc:LUMAZINESYN-MON. MetaCyc:LUMAZINESYN-MON. |
| BRENDA | 2.5.1.9. 246. |
Gene expression databases | |
| Genevestigator | P61714. |
Family and domain databases | |
| HAMAP | MF_00178. [Tree] |
| InterPro | IPR002180. DMRL_synthase. [Graphical view] |
| Gene3D | G3DSA:3.40.50.960. DMRL_synthase. 1 hit. |
| PANTHER | PTHR21058. DMRL_synthase. 1 hit. |
| Pfam | PF00885. DMRL_synthase. 1 hit. [Graphical view] |
| ProDom | PD003664. DMRL_synthase. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| TIGRFAMs | TIGR00114. lumazine-synth. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | RISB_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P61714 Secondary accession number(s): P25540, P77114, Q2MC11 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


