P61714 (RISB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6,7-dimethyl-8-ribityllumazine synthase
Short name=DMRL synthase
Short name=Lumazine synthase
EC=2.5.1.9

Alternative name(s):
Riboflavin synthase beta chain

Gene names

Name:	ribH
Synonyms:	ribE, ybaF
Ordered Locus Names:	b0415, JW0405

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes the condensation of 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate yielding 6,7-dimethyl-8-lumazine. HAMAP MF_00178
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine. HAMAP MF_00178
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2. HAMAP MF_00178
Subunit structure	Oligomer of 60 beta subunits forming an icosahedral capsid. The core of the capsid does not contain alpha subunits.
Sequence similarities	Belongs to the DMRL synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	riboflavin synthase complex Inferred from electronic annotation. Source: InterPro
Molecular function	riboflavin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 156	156	6,7-dimethyl-8-ribityllumazine synthase HAMAP MF_00178		PRO_0000134754

Sequences

	Sequence		Length	Mass (Da)
	P61714 [UniParc]. Last modified June 7, 2004. Version 1. Checksum: 1F8504B2892195C7	FASTA	156	16,157
	10 20 30 40 50 60 MNIIEANVAT PDARVAITIA RFNNFINDSL LEGAIDALKR IGQVKDENIT VVWVPGAYEL 70 80 90 100 110 120 PLAAGALAKT GKYDAVIALG TVIRGGTAHF EYVAGGASNG LAHVAQDSEI PVAFGVLTTE 130 140 150 SIEQAIERAG TKAGNKGAEA ALTALEMINV LKAIKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Insertional disruption of the nusB (ssyB) gene leads to cold-sensitive growth of Escherichia coli and suppression of the secY24 mutation." Taura T., Ueguchi C., Shiba K., Ito K. Mol. Gen. Genet. 234:429-432(1992) [PubMed: 1406588] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Biosynthesis of riboflavin. Lumazine synthase of Escherichia coli." Moertl S., Fischer M., Richter G., Tack J., Weinkauf S., Bacher A. J. Biol. Chem. 271:33201-33207(1996) [PubMed: 8969176] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X64395 Genomic DNA. Translation: CAA45736.1. U82664 Genomic DNA. Translation: AAB40171.1. U00096 Genomic DNA. Translation: AAC73518.1. AP009048 Genomic DNA. Translation: BAE76195.1.
PIR	S26202.
RefSeq	NP_414949.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P61714.
SMR	P61714. Positions 1-154.
ModBase	Search...
Protein-protein interaction databases
IntAct	P61714. 15 interactions.
MINT	MINT-1225919.
2D gel databases
SWISS-2DPAGE	P61714.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000003525; EBESCP00000003525; EBESCG00000002883. EBESCT00000014873; EBESCP00000014164; EBESCG00000013933.
GeneID	946453.
GenomeReviews	Gene locus JW0405 in contig AP009048_GR. Gene locus b0415 in contig U00096_GR.
KEGG	ecj:JW0405. eco:b0415.
PATRIC	32115979. VBIEscCol129921_0431.
Organism-specific databases
EchoBASE	EB1298.
EcoGene	EG11322. ribH.
Phylogenomic databases
eggNOG	COG0054.
GeneTree	EBGT00050000011214.
HOGENOM	HBG311126.
OMA	AGECSSG.
PhylomeDB	P61714.
ProtClustDB	PRK00061.
Enzyme and pathway databases
BioCyc	EcoCyc:LUMAZINESYN-MONOMER. MetaCyc:LUMAZINESYN-MONOMER.
BRENDA	2.5.1.9. 2026.
Gene expression databases
Genevestigator	P61714.
Family and domain databases
HAMAP	MF_00178. Lumazine_synth. [Tree]
InterPro	IPR002180. DMRL_synthase. [Graphical view]
Gene3D	G3DSA:3.40.50.960. DMRL_synthase. 1 hit.
KO	K00794.
PANTHER	PTHR21058. DMRL_synthase. 1 hit.
Pfam	PF00885. DMRL_synthase. 1 hit. [Graphical view]
SUPFAM	SSF52121. DMRL_synthase. 1 hit.
TIGRFAMs	TIGR00114. Lumazine-synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RISB_ECOLI

Accession

Primary (citable) accession number: P61714
Secondary accession number(s): P25540, P77114, Q2MC11

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	June 7, 2004
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program