Reviewed, UniProtKB/Swiss-Prot P61698 (SYA_BDEBA)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Bd0501

Organism

Bdellovibrio bacteriovorus [Complete proteome] [HAMAP]

Taxonomic identifier

959 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Bdellovibrionales › Bdellovibrionaceae › Bdellovibrio

Protein attributes

Sequence length	907 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm HAMAP MF_00036.
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 907	907	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000075065

Sequences

	Sequence		Length	Mass (Da)
	P61698-1 [UniParc]. Last modified June 7, 2004. Version 1. Checksum: 2B391C58F24BFDAB	FASTA	907	99,514
	10 20 30 40 50 60 MKSSEIRNAF IKYFEKNGHK VVPSSSLIPE NDPTLLFANA GMNQFKNTFL GLEKRDYSRA 70 80 90 100 110 120 VTAQKCVRAG GKHNDLENVG FTARHHTFFE MVGNFSFGDY FKKDAIHFAW EFLTKELAIP 130 140 150 160 170 180 KEKLYVTVHI SDDEAADIWH NQEGVPRERI FRFDKDNFWK MGDTGPCGPC TEIFYDHGPK 190 200 210 220 230 240 AGTISDPFKG IEAGEDRFVE IWNLVFMQYF ENPPGTLTPL PKPSVDTGGG LERMSAAMQG 250 260 270 280 290 300 VFNNYDTDLF QPMIQLACKI GNIEYISDKE VLAKNPAAAE VTSALRVLAD HCRSTSFLIA 310 320 330 340 350 360 DGALPSNEGR GYVLRRIMRR AIRYGRKLSA DKSFLPGMAE ALIESMGSVY PELKTRRDHI 370 380 390 400 410 420 LNTIRDEEDR FIATLDKGTA ILEDELKKAK SKGIKELSGE VVFRMYDTYG FPADLTRVIA 430 440 450 460 470 480 NEQGIEVNEA AFEKEMEDNR AKSKASWKGK SMGADEAHMI KFAKDYLQSG KSVTFLGYEG 490 500 510 520 530 540 TIGDGKVMGL SNGQAEVQEL KTGDTGLMIL NATTFYGEGG GQSGDVGYIM HDTNRARVIN 550 560 570 580 590 600 TTKIDDIVLH HVEIEHGSFK VGTAVVTGVD PVERRNTAAN HSATHLLHAA LRKVLGTHVT 610 620 630 640 650 660 QAGSLVDSQK TRFDFTHNKP VSSEEIKKIE DLVNEQIARC NPVQTEMMSH KAALEKGAMA 670 680 690 700 710 720 LFGEKYASDV RVLTMGDFSC ELCGGTHVKN TSEIRLFKIV SEAGVSSGVR RIEAITADNA 730 740 750 760 770 780 LQYMMSAVTH LDDALAAAGF QKSPHYIKHL ETTGETATLA NRVESLKDQV KQLEKEMKKL 790 800 810 820 830 840 QGGQVNVDDL AANALTFKTK AGASAKLVLA DVPLDDRQVL AEVTDHLKNK IQSGIVVVVG 850 860 870 880 890 900 QGDGSHPIIV SVSKEISGET KAGDLLKEVA GVMGGKGGGR PDFAQGAAPN RAQLNEAFSK VKSMLGL

References

[1]

"A predator unmasked: life cycle of Bdellovibrio bacteriovorus from a genomic perspective." Rendulic S., Jagtap P., Rosinus A., Eppinger M., Baar C., Lanz C., Keller H., Lambert C., Evans K.J., Goesmann A., Meyer F., Sockett R.E., Schuster S.C. Science 303:689-692(2004) [PubMed: 14752164] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15356 / HD100 / DSM 50701 / NCIB 9529.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842647 Genomic DNA. Translation: CAE78480.1.
RefSeq	NP_967487.1.
3D structure databases
SMR	P61698. Positions 1-480, 506-714.
ModBase	Search...
Genome annotation databases
GeneID	2733324.
KEGG	bba:Bd0501.
NMPDR	fig|264462.1.peg.457.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG354397.
OMA	GHTIEPS.
PhylomeDB	P61698.
Enzyme and pathway databases
BioCyc	BBAC264462:BD0501-MONOMER.
BRENDA	6.1.1.7. 3459.
Family and domain databases
HAMAP	MF_00036_B. Ala_tRNA_synth_B. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_BDEBA

Accession

Primary (citable) accession number: P61698

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	June 7, 2004
Last modified:	February 9, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)