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Protein

Alpha-2,8-sialyltransferase 8F

Gene

ST8SIA6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Prefers O-glycans to N-glycans or glycolipids as acceptor substrates. The minimal acceptor substrate is the NeuAc-alpha-2,36-Gal sequence at the non-reducing end of their carbohydrate groups (By similarity).By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei214 – 2141SubstrateBy similarity
Active sitei370 – 3701Proton donor/acceptorBy similarity

GO - Molecular functioni

  • alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity Source: Reactome
  • sialyltransferase activity Source: BHF-UCL

GO - Biological processi

  • carbohydrate biosynthetic process Source: BHF-UCL
  • ganglioside biosynthetic process Source: BHF-UCL
  • glycolipid biosynthetic process Source: BHF-UCL
  • glycoprotein metabolic process Source: BHF-UCL
  • oligosaccharide metabolic process Source: BHF-UCL
  • protein O-linked glycosylation Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-HSA-4085001. Sialic acid metabolism.
R-HSA-975577. N-Glycan antennae elongation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-2,8-sialyltransferase 8F (EC:2.4.99.-)
Alternative name(s):
Sialyltransferase 8F
Short name:
SIAT8-F
Sialyltransferase St8Sia VI
Short name:
ST8SiaVI
Gene namesi
Name:ST8SIA6
Synonyms:SIAT8F
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:23317. ST8SIA6.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 33CytoplasmicSequence analysis
Transmembranei4 – 2421Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini25 – 398374LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134909138.

Polymorphism and mutation databases

BioMutaiST8SIA6.
DMDMi48428578.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 398398Alpha-2,8-sialyltransferase 8FPRO_0000149299Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi66 – 661N-linked (GlcNAc...)Sequence analysis
Glycosylationi93 – 931N-linked (GlcNAc...)Sequence analysis
Glycosylationi151 – 1511N-linked (GlcNAc...)Sequence analysis
Disulfide bondi186 ↔ 335By similarity
Glycosylationi196 – 1961N-linked (GlcNAc...)Sequence analysis
Disulfide bondi200 ↔ 395By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP61647.
PeptideAtlasiP61647.
PRIDEiP61647.

PTM databases

iPTMnetiP61647.
PhosphoSiteiP61647.

Expressioni

Gene expression databases

BgeeiENSG00000148488.
CleanExiHS_ST8SIA6.
ExpressionAtlasiP61647. baseline and differential.

Organism-specific databases

HPAiHPA011635.

Interactioni

Protein-protein interaction databases

BioGridi130764. 1 interaction.
STRINGi9606.ENSP00000366827.

Structurei

3D structure databases

ProteinModelPortaliP61647.
SMRiP61647. Positions 115-395.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni236 – 2383Substrate bindingBy similarity
Regioni322 – 3243Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
GeneTreeiENSGT00550000074407.
HOGENOMiHOG000090201.
HOVERGENiHBG106106.
InParanoidiP61647.
KOiK06615.
OMAiNMSYEVE.
OrthoDBiEOG091G0CER.
PhylomeDBiP61647.
TreeFamiTF323961.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.

Sequencei

Sequence statusi: Complete.

P61647-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPGGALLAL LASLLLLLLL RLLWCPADAP GRARILVEES REATHGTPAA
60 70 80 90 100
LRTLRSPATA VPRATNSTYL NEKSLQLTEK CKNLQYGIES FSNKTKGYSE
110 120 130 140 150
NDYLQIITDI QSCPWKRQAE EYANFRAKLA SCCDAVQNFV VSQNNTPVGT
160 170 180 190 200
NMSYEVESKK EIPIKKNIFH MFPVSQPFVD YPYNQCAVVG NGGILNKSLC
210 220 230 240 250
GTEIDKSDFV FRCNLPPTTG DVSKDVGSKT NLVTINPSII TLKYGNLKEK
260 270 280 290 300
KALFLEDIAT YGDAFFLLPA FSFRANTGTS FKVYYTLEES KARQKVLFFH
310 320 330 340 350
PKYLKDLALF WRTKGVTAYR LSTGLMITSV AVELCKNVKL YGFWPFSKTV
360 370 380 390
EDIPVSHHYY DNKLPKHGFH QMPKEYSQIL QLHMKGILKL QFSKCEVA
Length:398
Mass (Da):44,836
Last modified:June 7, 2004 - v1
Checksum:i48F58B82E4B69A26
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ621583 mRNA. Translation: CAF21722.1.
EF445032 Genomic DNA. Translation: ACA06074.1.
EF445032 Genomic DNA. Translation: ACA06075.1.
AL158164, AL160289 Genomic DNA. Translation: CAI40658.1.
AL160289, AL158164 Genomic DNA. Translation: CAH70722.1.
BC137102 mRNA. Translation: AAI37103.1.
BC137103 mRNA. Translation: AAI37104.1.
CCDSiCCDS31158.1.
RefSeqiNP_001004470.1. NM_001004470.1.
UniGeneiHs.677766.

Genome annotation databases

EnsembliENST00000377602; ENSP00000366827; ENSG00000148488.
GeneIDi338596.
KEGGihsa:338596.
UCSCiuc001ipd.4. human.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

ST8Sia VI

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ621583 mRNA. Translation: CAF21722.1.
EF445032 Genomic DNA. Translation: ACA06074.1.
EF445032 Genomic DNA. Translation: ACA06075.1.
AL158164, AL160289 Genomic DNA. Translation: CAI40658.1.
AL160289, AL158164 Genomic DNA. Translation: CAH70722.1.
BC137102 mRNA. Translation: AAI37103.1.
BC137103 mRNA. Translation: AAI37104.1.
CCDSiCCDS31158.1.
RefSeqiNP_001004470.1. NM_001004470.1.
UniGeneiHs.677766.

3D structure databases

ProteinModelPortaliP61647.
SMRiP61647. Positions 115-395.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi130764. 1 interaction.
STRINGi9606.ENSP00000366827.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

PTM databases

iPTMnetiP61647.
PhosphoSiteiP61647.

Polymorphism and mutation databases

BioMutaiST8SIA6.
DMDMi48428578.

Proteomic databases

PaxDbiP61647.
PeptideAtlasiP61647.
PRIDEiP61647.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000377602; ENSP00000366827; ENSG00000148488.
GeneIDi338596.
KEGGihsa:338596.
UCSCiuc001ipd.4. human.

Organism-specific databases

CTDi338596.
GeneCardsiST8SIA6.
HGNCiHGNC:23317. ST8SIA6.
HPAiHPA011635.
MIMi610139. gene.
neXtProtiNX_P61647.
PharmGKBiPA134909138.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
GeneTreeiENSGT00550000074407.
HOGENOMiHOG000090201.
HOVERGENiHBG106106.
InParanoidiP61647.
KOiK06615.
OMAiNMSYEVE.
OrthoDBiEOG091G0CER.
PhylomeDBiP61647.
TreeFamiTF323961.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-HSA-4085001. Sialic acid metabolism.
R-HSA-975577. N-Glycan antennae elongation.

Miscellaneous databases

ChiTaRSiST8SIA6. human.
GenomeRNAii338596.
PROiP61647.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000148488.
CleanExiHS_ST8SIA6.
ExpressionAtlasiP61647. baseline and differential.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSIA8F_HUMAN
AccessioniPrimary (citable) accession number: P61647
Secondary accession number(s): B0YJ97, B9EH72, Q5VZH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: September 7, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.