P61647 (SIA8F_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-2,8-sialyltransferase 8F
EC=2.4.99.-

Alternative name(s):
Sialyltransferase 8F
Short name=SIAT8-F
Sialyltransferase St8Sia VI
Short name=ST8SiaVI

Gene names

Name:	ST8SIA6
Synonyms:	SIAT8F

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Prefers O-glycans to N-glycans or glycolipids as acceptor substrates. The minimal acceptor substrate is the NeuAc-alpha-2,36-Gal sequence at the non-reducing end of their carbohydrate groups By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the glycosyltransferase 29 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate biosynthetic process Inferred from sequence or structural similarity PubMed 11980897. Source: BHF-UCL ganglioside biosynthetic process Inferred from sequence or structural similarity PubMed 11980897. Source: BHF-UCL oligosaccharide metabolic process Inferred from sequence or structural similarity PubMed 11980897. Source: BHF-UCL post-translational protein modification Traceable author statement. Source: Reactome protein N-linked glycosylation via asparagine Traceable author statement. Source: Reactome protein O-linked glycosylation Inferred from sequence or structural similarity PubMed 11980897. Source: BHF-UCL
Cellular_component	Golgi membrane Traceable author statement. Source: Reactome integral to Golgi membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	sialyltransferase activity Inferred from sequence or structural similarity PubMed 11980897. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	Alpha-2,8-sialyltransferase 8F		PRO_0000149299
Regions
	Topological domain	1 – 3	3	Cytoplasmic Potential
	Transmembrane	4 – 24	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	25 – 398	374	Lumenal Potential
Amino acid modifications
	Glycosylation	66	1	N-linked (GlcNAc...) Potential
	Glycosylation	93	1	N-linked (GlcNAc...) Potential
	Glycosylation	151	1	N-linked (GlcNAc...) Potential
	Glycosylation	196	1	N-linked (GlcNAc...) Potential
	Disulfide bond	186 ↔ 335		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P61647 [UniParc]. Last modified June 7, 2004. Version 1. Checksum: 48F58B82E4B69A26	FASTA	398	44,836
	10 20 30 40 50 60 MRPGGALLAL LASLLLLLLL RLLWCPADAP GRARILVEES REATHGTPAA LRTLRSPATA 70 80 90 100 110 120 VPRATNSTYL NEKSLQLTEK CKNLQYGIES FSNKTKGYSE NDYLQIITDI QSCPWKRQAE 130 140 150 160 170 180 EYANFRAKLA SCCDAVQNFV VSQNNTPVGT NMSYEVESKK EIPIKKNIFH MFPVSQPFVD 190 200 210 220 230 240 YPYNQCAVVG NGGILNKSLC GTEIDKSDFV FRCNLPPTTG DVSKDVGSKT NLVTINPSII 250 260 270 280 290 300 TLKYGNLKEK KALFLEDIAT YGDAFFLLPA FSFRANTGTS FKVYYTLEES KARQKVLFFH 310 320 330 340 350 360 PKYLKDLALF WRTKGVTAYR LSTGLMITSV AVELCKNVKL YGFWPFSKTV EDIPVSHHYY 370 380 390 DNKLPKHGFH QMPKEYSQIL QLHMKGILKL QFSKCEVA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and expression of a sixth human alpha2,8-sialyltransferase (hST8SiaVI)." Teintenier-Lelievre M., Delannoy P., Harduin-Lepers A. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	NHLBI resequencing and genotyping service (RS&G) Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

ST8Sia VI

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ621583 mRNA. Translation: CAF21722.1. EF445032 Genomic DNA. Translation: ACA06074.1. EF445032 Genomic DNA. Translation: ACA06075.1. AL158164, AL160289 Genomic DNA. Translation: CAI40658.1. AL160289, AL158164 Genomic DNA. Translation: CAH70722.1. BC137102 mRNA. Translation: AAI37103.1. BC137103 mRNA. Translation: AAI37104.1.
IPI	IPI00259856.
RefSeq	NP_001004470.1. NM_001004470.1.
UniGene	Hs.677766.
3D structure databases
ProteinModelPortal	P61647.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000366827.
Protein family/group databases
CAZy	GT29. Glycosyltransferase Family 29.
PTM databases
PhosphoSite	P61647.
Polymorphism databases
DMDM	48428578.
Proteomic databases
PaxDb	P61647.
PRIDE	P61647.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000377602; ENSP00000366827; ENSG00000148488.
GeneID	338596.
KEGG	hsa:338596.
UCSC	uc001ipd.3. human.
Organism-specific databases
CTD	338596.
GeneCards	GC10M017360.
HGNC	HGNC:23317. ST8SIA6.
HPA	HPA011635.
MIM	610139. gene.
neXtProt	NX_P61647.
PharmGKB	PA134909138.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG323775.
HOGENOM	HOG000090201.
HOVERGEN	HBG106106.
InParanoid	P61647.
KO	K06615.
OMA	NMSYEVE.
PhylomeDB	P61647.
Enzyme and pathway databases
Reactome	REACT_17015. Metabolism of proteins.
UniPathway	UPA00378.
Gene expression databases
ArrayExpress	P61647.
Bgee	P61647.
CleanEx	HS_ST8SIA6.
Genevestigator	P61647.
Family and domain databases
InterPro	IPR001675. Glyco_trans_29. IPR012163. Sialyl_trans. [Graphical view]
Pfam	PF00777. Glyco_transf_29. 1 hit. [Graphical view]
PIRSF	PIRSF005557. Sialyl_trans. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	ST8SIA6. human.
GenomeRNAi	338596.
NextBio	97053.
SOURCE	Search...

Entry information

Entry name

SIA8F_HUMAN

Accession

Primary (citable) accession number: P61647
Secondary accession number(s): B0YJ97, B9EH72, Q5VZH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	June 7, 2004
Last modified:	April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.