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P61647 (SIA8F_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-2,8-sialyltransferase 8F

EC=2.4.99.-
Alternative name(s):
Sialyltransferase 8F
Short name=SIAT8-F
Sialyltransferase St8Sia VI
Short name=ST8SiaVI
Gene names
Name:ST8SIA6
Synonyms:SIAT8F
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length398 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Prefers O-glycans to N-glycans or glycolipids as acceptor substrates. The minimal acceptor substrate is the NeuAc-alpha-2,36-Gal sequence at the non-reducing end of their carbohydrate groups By similarity.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein Potential.

Sequence similarities

Belongs to the glycosyltransferase 29 family.

Ontologies

Keywords
   Cellular componentGolgi apparatus
Membrane
   DomainSignal-anchor
Transmembrane
Transmembrane helix
   Molecular functionGlycosyltransferase
Transferase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate biosynthetic process

Inferred from sequence or structural similarity PubMed 11980897. Source: BHF-UCL

cellular protein metabolic process

Traceable author statement. Source: Reactome

ganglioside biosynthetic process

Inferred from sequence or structural similarity PubMed 11980897. Source: BHF-UCL

glycolipid biosynthetic process

Inferred from sequence or structural similarity PubMed 11980897. Source: BHF-UCL

glycoprotein metabolic process

Inferred from sequence or structural similarity PubMed 11980897. Source: BHF-UCL

oligosaccharide metabolic process

Inferred from sequence or structural similarity PubMed 11980897. Source: BHF-UCL

post-translational protein modification

Traceable author statement. Source: Reactome

protein N-linked glycosylation via asparagine

Traceable author statement. Source: Reactome

protein O-linked glycosylation

Inferred from sequence or structural similarity PubMed 11980897. Source: BHF-UCL

sialylation

Inferred from sequence or structural similarity PubMed 11980897. Source: GOC

   Cellular_componentGolgi membrane

Traceable author statement. Source: Reactome

integral component of Golgi membrane

Inferred from electronic annotation. Source: InterPro

   Molecular_functionsialyltransferase activity

Inferred from sequence or structural similarity PubMed 11980897. Source: BHF-UCL

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 398398Alpha-2,8-sialyltransferase 8F
PRO_0000149299

Regions

Topological domain1 – 33Cytoplasmic Potential
Transmembrane4 – 2421Helical; Signal-anchor for type II membrane protein; Potential
Topological domain25 – 398374Lumenal Potential

Amino acid modifications

Glycosylation661N-linked (GlcNAc...) Potential
Glycosylation931N-linked (GlcNAc...) Potential
Glycosylation1511N-linked (GlcNAc...) Potential
Glycosylation1961N-linked (GlcNAc...) Potential
Disulfide bond186 ↔ 335 By similarity

Sequences

Sequence LengthMass (Da)Tools
P61647 [UniParc].

Last modified June 7, 2004. Version 1.
Checksum: 48F58B82E4B69A26

FASTA39844,836
        10         20         30         40         50         60 
MRPGGALLAL LASLLLLLLL RLLWCPADAP GRARILVEES REATHGTPAA LRTLRSPATA 

        70         80         90        100        110        120 
VPRATNSTYL NEKSLQLTEK CKNLQYGIES FSNKTKGYSE NDYLQIITDI QSCPWKRQAE 

       130        140        150        160        170        180 
EYANFRAKLA SCCDAVQNFV VSQNNTPVGT NMSYEVESKK EIPIKKNIFH MFPVSQPFVD 

       190        200        210        220        230        240 
YPYNQCAVVG NGGILNKSLC GTEIDKSDFV FRCNLPPTTG DVSKDVGSKT NLVTINPSII 

       250        260        270        280        290        300 
TLKYGNLKEK KALFLEDIAT YGDAFFLLPA FSFRANTGTS FKVYYTLEES KARQKVLFFH 

       310        320        330        340        350        360 
PKYLKDLALF WRTKGVTAYR LSTGLMITSV AVELCKNVKL YGFWPFSKTV EDIPVSHHYY 

       370        380        390 
DNKLPKHGFH QMPKEYSQIL QLHMKGILKL QFSKCEVA 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and expression of a sixth human alpha2,8-sialyltransferase (hST8SiaVI)."
Teintenier-Lelievre M., Delannoy P., Harduin-Lepers A.
Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]NHLBI resequencing and genotyping service (RS&G)
Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The DNA sequence and comparative analysis of human chromosome 10."
Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. expand/collapse author list , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
+Additional computationally mapped references.

Web resources

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ621583 mRNA. Translation: CAF21722.1.
EF445032 Genomic DNA. Translation: ACA06074.1.
EF445032 Genomic DNA. Translation: ACA06075.1.
AL158164, AL160289 Genomic DNA. Translation: CAI40658.1.
AL160289, AL158164 Genomic DNA. Translation: CAH70722.1.
BC137102 mRNA. Translation: AAI37103.1.
BC137103 mRNA. Translation: AAI37104.1.
RefSeqNP_001004470.1. NM_001004470.1.
UniGeneHs.677766.

3D structure databases

ProteinModelPortalP61647.
SMRP61647. Positions 182-384.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid130764. 1 interaction.
STRING9606.ENSP00000366827.

Protein family/group databases

CAZyGT29. Glycosyltransferase Family 29.

PTM databases

PhosphoSiteP61647.

Polymorphism databases

DMDM48428578.

Proteomic databases

PaxDbP61647.
PRIDEP61647.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000377602; ENSP00000366827; ENSG00000148488.
GeneID338596.
KEGGhsa:338596.
UCSCuc001ipd.3. human.

Organism-specific databases

CTD338596.
GeneCardsGC10M017360.
HGNCHGNC:23317. ST8SIA6.
HPAHPA011635.
MIM610139. gene.
neXtProtNX_P61647.
PharmGKBPA134909138.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG323775.
HOGENOMHOG000090201.
HOVERGENHBG106106.
InParanoidP61647.
KOK06615.
OMANMSYEVE.
PhylomeDBP61647.
TreeFamTF323961.

Enzyme and pathway databases

ReactomeREACT_17015. Metabolism of proteins.
UniPathwayUPA00378.

Gene expression databases

BgeeP61647.
CleanExHS_ST8SIA6.
GenevestigatorP61647.

Family and domain databases

InterProIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFPIRSF005557. Sialyl_trans. 1 hit.
ProtoNetSearch...

Other

ChiTaRSST8SIA6. human.
GenomeRNAi338596.
NextBio97053.
PROP61647.
SOURCESearch...

Entry information

Entry nameSIA8F_HUMAN
AccessionPrimary (citable) accession number: P61647
Secondary accession number(s): B0YJ97, B9EH72, Q5VZH4
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: April 16, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 10

Human chromosome 10: entries, gene names and cross-references to MIM