Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P61619 (S61A1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein transport protein Sec61 subunit alpha isoform 1

Short name=Sec61 alpha-1
Gene names
Name:SEC61A1
Synonyms:SEC61A
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length476 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a crucial role in the insertion of secretory and membrane polypeptides into the ER. Required for assembly of membrane and secretory proteins. Tightly associated with membrane-bound ribosomes, either directly or through adapter proteins.

Subunit structure

Heterotrimeric complex composed of SEC61-alpha, SEC61-beta and SEC61-gamma.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the SecY/SEC61-alpha family.

Sequence caution

The sequence BAC11283.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAC11434.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein transport
Translocation
Transport
   Cellular componentEndoplasmic reticulum
Membrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processSRP-dependent cotranslational protein targeting to membrane

Inferred from mutant phenotype PubMed 22375059. Source: MGI

antigen processing and presentation of exogenous peptide antigen via MHC class I

Traceable author statement. Source: Reactome

antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent

Traceable author statement. Source: Reactome

antigen processing and presentation of peptide antigen via MHC class I

Traceable author statement. Source: Reactome

cell growth

Inferred from mutant phenotype PubMed 22375059. Source: MGI

endoplasmic reticulum organization

Inferred from mutant phenotype PubMed 22375059. Source: MGI

posttranslational protein targeting to membrane

Inferred from mutant phenotype PubMed 22375059. Source: MGI

protein targeting to ER

Inferred from sequence or structural similarity. Source: UniProtKB

response to interferon-gamma

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentintegral component of endoplasmic reticulum membrane

Inferred from sequence or structural similarity. Source: UniProtKB

membrane

Inferred from direct assay PubMed 22375059. Source: MGI

rough endoplasmic reticulum

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionribosome binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CANXP278243EBI-358919,EBI-355947
HSPA5P110213EBI-358919,EBI-354921

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P61619-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 3 (identifier: P61619-3)

The sequence of this isoform differs from the canonical sequence as follows:
     2-120: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 476475Protein transport protein Sec61 subunit alpha isoform 1
PRO_0000131791

Regions

Topological domain2 – 3332Cytoplasmic Potential
Transmembrane34 – 5320Helical; Potential
Topological domain54 – 7623Lumenal Potential
Transmembrane77 – 9620Helical; Potential
Topological domain97 – 11721Cytoplasmic Potential
Transmembrane118 – 13821Helical; Potential
Topological domain139 – 1446Lumenal Potential
Transmembrane145 – 16521Helical; Potential
Topological domain166 – 1727Cytoplasmic Potential
Transmembrane173 – 19321Helical; Potential
Topological domain194 – 24047Lumenal Potential
Transmembrane241 – 26121Helical; Potential
Topological domain262 – 28827Cytoplasmic Potential
Transmembrane289 – 30921Helical; Potential
Topological domain310 – 35445Lumenal Potential
Transmembrane355 – 37521Helical; Potential
Topological domain376 – 42045Cytoplasmic Potential
Transmembrane421 – 44121Helical; Potential
Topological domain442 – 4454Lumenal Potential
Transmembrane446 – 46217Helical; Potential
Topological domain463 – 47614Cytoplasmic Potential

Natural variations

Alternative sequence2 – 120119Missing in isoform 3.
VSP_013747

Experimental info

Sequence conflict1351G → W in AAD27765. Ref.1
Sequence conflict2341F → L in BAC11298. Ref.4
Sequence conflict2781T → S in AAD27765. Ref.1
Sequence conflict3101A → V in AAD27765. Ref.1
Sequence conflict3161L → F in AAD27765. Ref.1
Sequence conflict3191S → N in AAD27765. Ref.1
Sequence conflict323 – 3297TWSDTSS → QWADVSG in AAD27765. Ref.1
Sequence conflict3351A → S in AAD27765. Ref.1
Sequence conflict351 – 3555FGSVL → MGAIF in AAD27765. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 44A9B7B37C7C82E4

FASTA47652,265
        10         20         30         40         50         60 
MAIKFLEVIK PFCVILPEIQ KPERKIQFKE KVLWTAITLF IFLVCCQIPL FGIMSSDSAD 

        70         80         90        100        110        120 
PFYWMRVILA SNRGTLMELG ISPIVTSGLI MQLLAGAKII EVGDTPKDRA LFNGAQKLFG 

       130        140        150        160        170        180 
MIITIGQSIV YVMTGMYGDP SEMGAGICLL ITIQLFVAGL IVLLLDELLQ KGYGLGSGIS 

       190        200        210        220        230        240 
LFIATNICET IVWKAFSPTT VNTGRGMEFE GAIIALFHLL ATRTDKVRAL REAFYRQNLP 

       250        260        270        280        290        300 
NLMNLIATIF VFAVVIYFQG FRVDLPIKSA RYRGQYNTYP IKLFYTSNIP IILQSALVSN 

       310        320        330        340        350        360 
LYVISQMLSA RFSGNLLVSL LGTWSDTSSG GPARAYPVGG LCYYLSPPES FGSVLEDPVH 

       370        380        390        400        410        420 
AVVYIVFMLG SCAFFSKTWI EVSGSSAKDV AKQLKEQQMV MRGHRETSMV HELNRYIPTA 

       430        440        450        460        470 
AAFGGLCIGA LSVLADFLGA IGSGTGILLA VTIIYQYFEI FVKEQSEVGS MGALLF 

« Hide

Isoform 3 [UniParc].

Checksum: A3C282498CE920DF
Show »

FASTA35738,980

References

« Hide 'large scale' references
[1]"Human sec61 homolog gene."
Liu T., Zhang J., Ye M., Zhang Q., Fu G., Zhou J., Wu J., Shen Y., Yu M., Chen S., Mao M., Chen Z.
Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Human sec61 homolog gene mRNA sequence."
Li W., Liu T., Fu G., Zhang Q., Ye M., Zhou J., Wu J., Shen Y., Yu M., Chen S., Mao M., Chen Z.
Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"Sec61 alpha isoforms."
Finke K., Prehn S., Hartmann E.
Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Placenta and Teratocarcinoma.
[5]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Bone marrow and Lymph node.
[6]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF077032 mRNA. Translation: AAD27765.1.
AF084458 mRNA. Translation: AAD39847.1.
AF346602 mRNA. Translation: AAK29083.1.
AK074907 mRNA. Translation: BAC11283.1. Different initiation.
AK074928 mRNA. Translation: BAC11298.1.
AK075148 mRNA. Translation: BAC11434.1. Different initiation.
AL831940 mRNA. Translation: CAD38592.1.
AL832821 mRNA. Translation: CAI46127.1.
RefSeqNP_037468.1. NM_013336.3.
UniGeneHs.518236.

3D structure databases

ProteinModelPortalP61619.
SMRP61619. Positions 1-476.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid118968. 26 interactions.
IntActP61619. 18 interactions.
MINTMINT-1133988.

Protein family/group databases

TCDB3.A.5.9.1. the general secretory pathway (sec) family.

PTM databases

PhosphoSiteP61619.

Polymorphism databases

DMDM48429109.

Proteomic databases

PaxDbP61619.
PeptideAtlasP61619.
PRIDEP61619.

Protocols and materials databases

DNASU29927.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000243253; ENSP00000243253; ENSG00000058262. [P61619-1]
ENST00000424880; ENSP00000411445; ENSG00000058262. [P61619-3]
GeneID29927.
KEGGhsa:29927.
UCSCuc003ekb.3. human. [P61619-1]

Organism-specific databases

CTD29927.
GeneCardsGC03P127770.
HGNCHGNC:18276. SEC61A1.
HPACAB022076.
HPA045070.
MIM609213. gene.
neXtProtNX_P61619.
PharmGKBPA134901772.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0201.
HOVERGENHBG055271.
InParanoidP61619.
KOK10956.
PhylomeDBP61619.
TreeFamTF300348.

Enzyme and pathway databases

ReactomeREACT_17015. Metabolism of proteins.
REACT_6900. Immune System.
REACT_71. Gene Expression.

Gene expression databases

ArrayExpressP61619.
BgeeP61619.
GenevestigatorP61619.

Family and domain databases

Gene3D1.10.3370.10. 1 hit.
InterProIPR002208. SecY/SEC61-alpha.
IPR023201. SecY_su_dom.
IPR019561. Translocon_Sec61/SecY_plug_dom.
[Graphical view]
PANTHERPTHR10906. PTHR10906. 1 hit.
PfamPF10559. Plug_translocon. 1 hit.
PF00344. SecY. 1 hit.
[Graphical view]
PIRSFPIRSF004557. SecY. 1 hit.
SUPFAMSSF103491. SSF103491. 1 hit.
TIGRFAMsTIGR00967. 3a0501s007. 1 hit.
PROSITEPS00755. SECY_1. 1 hit.
PS00756. SECY_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSEC61A1. human.
GeneWikiSec61_alpha_1.
GenomeRNAi29927.
NextBio52551.
PROP61619.
SOURCESearch...

Entry information

Entry nameS61A1_HUMAN
AccessionPrimary (citable) accession number: P61619
Secondary accession number(s): P38378 expand/collapse secondary AC list , P57726, Q5JPF8, Q8N0Z4, Q8N3U3, Q8NC71, Q9BU16, Q9Y2R3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM