Reviewed, UniProtKB/Swiss-Prot P61619 (S61A1_HUMAN)

Names and origin

Protein names

Recommended name:
    Protein transport protein Sec61 subunit alpha isoform 1
      Short name=Sec61 alpha-1

Gene names

Name:	SEC61A1
Synonyms:	SEC61A

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plays a crucial role in the insertion of secretory and membrane polypeptides into the ER. Required for assembly of membrane and secretory proteins. Tightly associated with membrane-bound ribosomes, either directly or through adapter proteins.
Subunit structure	Heterotrimeric complex composed of SEC61-alpha, SEC61-beta and SEC61-gamma.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the secY/SEC61-alpha family.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein targeting to ER Inferred from sequence or structural similarity. Source: UniProtKB transmembrane transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to endoplasmic reticulum membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	P-P-bond-hydrolysis-driven protein transmembrane transporter activity Inferred from electronic annotation. Source: InterPro ribosome binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AP1S1	P61966	1	EBI-358919,EBI-516199
NDN	Q99608	1	EBI-358919,EBI-718177

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P61619-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 3 (identifier: P61619-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-120: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 476	475	Protein transport protein Sec61 subunit alpha isoform 1		PRO_0000131791
Regions
	Topological domain	2 – 33	32	Cytoplasmic Potential
	Transmembrane	34 – 53	20	Potential
	Topological domain	54 – 76	23	Lumenal Potential
	Transmembrane	77 – 96	20	Potential
	Topological domain	97 – 117	21	Cytoplasmic Potential
	Transmembrane	118 – 138	21	Potential
	Topological domain	139 – 144	6	Lumenal Potential
	Transmembrane	145 – 165	21	Potential
	Topological domain	166 – 172	7	Cytoplasmic Potential
	Transmembrane	173 – 193	21	Potential
	Topological domain	194 – 240	47	Lumenal Potential
	Transmembrane	241 – 261	21	Potential
	Topological domain	262 – 288	27	Cytoplasmic Potential
	Transmembrane	289 – 309	21	Potential
	Topological domain	310 – 354	45	Lumenal Potential
	Transmembrane	355 – 375	21	Potential
	Topological domain	376 – 420	45	Cytoplasmic Potential
	Transmembrane	421 – 441	21	Potential
	Topological domain	442 – 445	4	Lumenal Potential
	Transmembrane	446 – 462	17	Potential
	Topological domain	463 – 476	14	Cytoplasmic Potential
Natural variations
	Alternative sequence	1 – 120	120	Missing in isoform 3.		VSP_013747
Experimental info
	Sequence conflict	135	1	G → W in AAD27765. Ref.1
	Sequence conflict	234	1	F → L in BAC11298. Ref.4
	Sequence conflict	278	1	T → S in AAD27765. Ref.1
	Sequence conflict	310	1	A → V in AAD27765. Ref.1
	Sequence conflict	316	1	L → F in AAD27765. Ref.1
	Sequence conflict	319	1	S → N in AAD27765. Ref.1
	Sequence conflict	323 – 329	7	TWSDTSS → QWADVSG in AAD27765. Ref.1
	Sequence conflict	335	1	A → S in AAD27765. Ref.1
	Sequence conflict	351 – 355	5	FGSVL → MGAIF in AAD27765. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 44A9B7B37C7C82E4	FASTA	476	52,265
	10 20 30 40 50 60 MAIKFLEVIK PFCVILPEIQ KPERKIQFKE KVLWTAITLF IFLVCCQIPL FGIMSSDSAD 70 80 90 100 110 120 PFYWMRVILA SNRGTLMELG ISPIVTSGLI MQLLAGAKII EVGDTPKDRA LFNGAQKLFG 130 140 150 160 170 180 MIITIGQSIV YVMTGMYGDP SEMGAGICLL ITIQLFVAGL IVLLLDELLQ KGYGLGSGIS 190 200 210 220 230 240 LFIATNICET IVWKAFSPTT VNTGRGMEFE GAIIALFHLL ATRTDKVRAL REAFYRQNLP 250 260 270 280 290 300 NLMNLIATIF VFAVVIYFQG FRVDLPIKSA RYRGQYNTYP IKLFYTSNIP IILQSALVSN 310 320 330 340 350 360 LYVISQMLSA RFSGNLLVSL LGTWSDTSSG GPARAYPVGG LCYYLSPPES FGSVLEDPVH 370 380 390 400 410 420 AVVYIVFMLG SCAFFSKTWI EVSGSSAKDV AKQLKEQQMV MRGHRETSMV HELNRYIPTA 430 440 450 460 470 AAFGGLCIGA LSVLADFLGA IGSGTGILLA VTIIYQYFEI FVKEQSEVGS MGALLF
	Isoform 3. Checksum: 51804242521CDA10	FASTA	356	38,849
	10 20 30 40 50 60 MIITIGQSIV YVMTGMYGDP SEMGAGICLL ITIQLFVAGL IVLLLDELLQ KGYGLGSGIS 70 80 90 100 110 120 LFIATNICET IVWKAFSPTT VNTGRGMEFE GAIIALFHLL ATRTDKVRAL REAFYRQNLP 130 140 150 160 170 180 NLMNLIATIF VFAVVIYFQG FRVDLPIKSA RYRGQYNTYP IKLFYTSNIP IILQSALVSN 190 200 210 220 230 240 LYVISQMLSA RFSGNLLVSL LGTWSDTSSG GPARAYPVGG LCYYLSPPES FGSVLEDPVH 250 260 270 280 290 300 AVVYIVFMLG SCAFFSKTWI EVSGSSAKDV AKQLKEQQMV MRGHRETSMV HELNRYIPTA 310 320 330 340 350 AAFGGLCIGA LSVLADFLGA IGSGTGILLA VTIIYQYFEI FVKEQSEVGS MGALLF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human sec61 homolog gene." Liu T., Zhang J., Ye M., Zhang Q., Fu G., Zhou J., Wu J., Shen Y., Yu M., Chen S., Mao M., Chen Z. Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Human sec61 homolog gene mRNA sequence." Li W., Liu T., Fu G., Zhang Q., Ye M., Zhou J., Wu J., Shen Y., Yu M., Chen S., Mao M., Chen Z. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Sec61 alpha isoforms." Finke K., Prehn S., Hartmann E. Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Placenta and Teratocarcinoma.
[5]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Bone marrow and Lymph node.
[6]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF077032 mRNA. Translation: AAD27765.1. AF084458 mRNA. Translation: AAD39847.1. AF346602 mRNA. Translation: AAK29083.1. AK074907 mRNA. Translation: BAC11283.1. Different initiation. AK074928 mRNA. Translation: BAC11298.1. AK075148 mRNA. Translation: BAC11434.1. Different initiation. AL831940 mRNA. Translation: CAD38592.1. AL832821 mRNA. Translation: CAI46127.1.
IPI	IPI00604476. IPI00941172.
RefSeq	NP_037468.1.
UniGene	Hs.518236
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P61619. 10 interactions.
STRING	P61619.
Protein family/group databases
TCDB	3.A.5.9.1. general secretory pathway (Sec) family.
Proteomic databases
PeptideAtlas	P61619.
PRIDE	P61619.
Genome annotation databases
Ensembl	ENST00000243253; ENSP00000243253; ENSG00000058262; Homo sapiens. [Genome view]
GeneID	29927.
KEGG	hsa:29927.
UCSC	uc003ekb.1. human. uc003ekc.1. human.
Organism-specific databases
CTD	29927.
GeneCards	GC03P129253.
HGNC	HGNC:18276. SEC61A1.
HPA	CAB022076.
MIM	609213. gene.
PharmGKB	PA134901772.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	P61619.
InParanoid	P61619.
Enzyme and pathway databases
Reactome	REACT_15380. Diabetes pathways.
Gene expression databases
ArrayExpress	P61619.
Bgee	P61619.
Genevestigator	P61619.
GermOnline	ENSG00000058262. Homo sapiens.
Family and domain databases
InterPro	IPR002208. SecY. IPR019561. Translocon_Sec61/SecY_plug_dom. [Graphical view]
PANTHER	PTHR10906. SecY. 1 hit.
Pfam	PF10559. Plug_translocon. 1 hit. PF00344. SecY. 1 hit. [Graphical view]
PIRSF	PIRSF004557. SecY. 1 hit.
PROSITE	PS00755. SECY_1. 1 hit. PS00756. SECY_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	52551.
SOURCE	Search...

Entry information

Entry name

S61A1_HUMAN

Accession

Primary (citable) accession number: P61619
Secondary accession number(s): P38378 , P57726, Q5JPF8, Q8N0Z4, Q8N3U3, Q8NC71, Q9BU16, Q9Y2R3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	January 23, 2007
Last modified:	December 15, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.