Reviewed, UniProtKB/Swiss-Prot P61609 (LEXA2_GEOSL)

Names and origin

Protein names

Recommended name:
    LexA repressor 2
    EC=3.4.21.88

Gene names

Name:	lexA2
Synonyms:	lexA-2
Ordered Locus Names:	GSU1617

Organism

Geobacter sulfurreducens [Complete proteome] [HAMAP]

Taxonomic identifier

35554 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfuromonadales › Geobacteraceae › Geobacter

Protein attributes

Sequence length	203 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity. HAMAP MF_00015
Catalytic activity	Hydrolysis of Ala-|-Gly bond in repressor lexA. HAMAP MF_00015
Subunit structure	Homodimer By similarity. HAMAP MF_00015
Sequence similarities	Belongs to the peptidase S24 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication SOS response Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Hydrolase Repressor
PTM	Autocatalytic cleavage
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP proteolysis Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: HAMAP serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 203	203	LexA repressor 2 HAMAP MF_00015		PRO_0000170038
Regions
	DNA binding	28 – 48	21	H-T-H motif By similarity
Sites
	Active site	122	1	For autocatalytic cleavage activity By similarity
	Active site	159	1	For autocatalytic cleavage activity By similarity
	Site	88 – 89	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P61609-1 [UniParc]. Last modified May 24, 2004. Version 1. Checksum: 87BD092817947033	FASTA	203	22,445
	10 20 30 40 50 60 METLTSRQRT VLEFITAHVD RHGYPPTMRE IARHLNVNGT LGVAKHLEAL ARKGYLQREP 70 80 90 100 110 120 GNSRGITLTG QTRHTPTVSL PVIGVVRAGV PQIATEEIEE HISIDQSLVK GGAFFLRVKG 130 140 150 160 170 180 DSMINAAIIE GDLALVRPQQ TAENRDIVVA MIDGEATLKR FYQGADHIRL QPENPNMAPI 190 200 IVRSGVQEAW IIGKVVGIYR RMD

References

[1]

"Genome of Geobacter sulfurreducens: metal reduction in subsurface environments." Methe B.A., Nelson K.E., Eisen J.A., Paulsen I.T., Nelson W.C., Heidelberg J.F., Wu D., Wu M., Ward N.L., Beanan M.J., Dodson R.J., Madupu R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn M.L., Kolonay J.F. , Sullivan S.A., Haft D.H., Selengut J., Davidsen T.M., Zafar N., White O., Tran B., Romero C., Forberger H.A., Weidman J.F., Khouri H.M., Feldblyum T.V., Utterback T.R., Van Aken S.E., Lovley D.R., Fraser C.M. Science 302:1967-1969(2003) [PubMed: 14671304] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51573 / DSM 12127 / PCA.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017180 Genomic DNA. Translation: AAR34991.1.
RefSeq	NP_952668.1.
3D structure databases
SMR	P61609. Positions 80-203.
ModBase	Search...
Protein family/group databases
MEROPS	S24.001.
Genome annotation databases
GeneID	2685264.
GenomeReviews	Gene locus GSU1617 in contig AE017180_GR.
KEGG	gsu:GSU1617.
NMPDR	fig|243231.1.peg.1606.
TIGR	GSU1617.
Phylogenomic databases
HOGENOM	HBG679610.
OMA	TAQQELY.
Enzyme and pathway databases
BioCyc	GSUL243231:GSU_1617-MONOMER.
BRENDA	3.4.21.88. 276898.
Family and domain databases
HAMAP	MF_00015. LexA. [Tree]
InterPro	IPR006199. LexA_DNA-bd_dom. IPR006200. Pept_S24_LexA. IPR006197. Peptidase_S24_LexA_cons-reg. IPR019759. Peptidase_S24_S26_cons-reg. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF01726. LexA_DNA_bind. 1 hit. PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00726. LEXASERPTASE.
TIGRFAMs	TIGR00498. lexA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEXA2_GEOSL

Accession

Primary (citable) accession number: P61609

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	May 24, 2004
Last modified:	February 9, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)