Reviewed, UniProtKB/Swiss-Prot P61605 (LEXA_RHIRD)

Names and origin

Protein names

Recommended name:
    LexA repressor
    EC=3.4.21.88

Gene names

Name:

lexA

Organism

Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium radiobacter)

Taxonomic identifier

358 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity. HAMAP MF_00015
Catalytic activity	Hydrolysis of Ala-|-Gly bond in repressor lexA. HAMAP MF_00015
Subunit structure	Homodimer By similarity. HAMAP MF_00015
Sequence similarities	Belongs to the peptidase S24 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication SOS response Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Hydrolase Repressor
PTM	Autocatalytic cleavage
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP proteolysis Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: HAMAP serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 239	239	LexA repressor HAMAP MF_00015		PRO_0000169999
Regions
	DNA binding	27 – 47	21	H-T-H motif By similarity
Sites
	Active site	159	1	For autocatalytic cleavage activity By similarity
	Active site	197	1	For autocatalytic cleavage activity By similarity
	Site	124 – 125	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P61605-1 [UniParc]. Last modified May 24, 2004. Version 1. Checksum: 7593331A8E06D52A	FASTA	239	25,622
	10 20 30 40 50 60 MLTRKQQELL LFIHGTNEGN PGVPPSFDEM KDALDLASKS GIHRLITALE ERGFIRRLPN 70 80 90 100 110 120 RARALEVIKL PEAYAGASQV RRGFSPTVIE GSLGKLASPP PAPKPAPPAE AASVAVPVMG 130 140 150 160 170 180 RIAAGVPISA IQNNMHDISV PVEMIGSGEH YALEIKGDSM IEAGILDGDT VIIRNGSTAS 190 200 210 220 230 PGDIVVALID DEEATLKRFR RKGASIALEA ANPAYETRIF GPDRVKIQGR LVGLIRRYH

References

[1]	"The lexA gene of Agrobacterium tumefaciens." Campoy S., Tapias A., Mazon G., Barbe J. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF251129 Genomic DNA. Translation: AAQ14270.1.
3D structure databases
SMR	P61605. Positions 115-239.
ModBase	Search...
Protein family/group databases
MEROPS	S24.001.
Enzyme and pathway databases
BRENDA	3.4.21.88. 2199.
Family and domain databases
HAMAP	MF_00015. LexA. [Tree]
InterPro	IPR006199. LexA_DNA-bd_dom. IPR006200. Pept_S24_LexA. IPR006197. Peptidase_S24_LexA_cons-reg. IPR019759. Peptidase_S24_S26_cons-reg. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF01726. LexA_DNA_bind. 1 hit. PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00726. LEXASERPTASE.
TIGRFAMs	TIGR00498. lexA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEXA_RHIRD

Accession

Primary (citable) accession number: P61605

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	May 24, 2004
Last modified:	February 9, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)