P61604 (CH10_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
10 kDa heat shock protein, mitochondrial
Short name=Hsp10

Alternative name(s):
10 kDa chaperonin
Chaperonin 10
Short name=CPN10
Early-pregnancy factor
Short name=EPF

Gene names

Name:

HSPE1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	102 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.
Subunit structure	Homohexamer.
Subcellular location	Mitochondrion matrix.
Induction	By stress.
Sequence similarities	Belongs to the GroES chaperonin family.
Mass spectrometry	Molecular mass is 10843.5±0.2 Da from positions 2 - 102. Determined by ESI. Ref.11

Ontologies

Keywords
Biological process	Stress response
Cellular component	Mitochondrion
Molecular function	Chaperone
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	activation of cysteine-type endopeptidase activity involved in apoptotic process Non-traceable author statement. Source: UniProtKB protein folding Traceable author statement. Source: ProtInc response to unfolded protein Traceable author statement. Source: ProtInc
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro chaperone binding Inferred from physical interaction. Source: UniProtKB unfolded protein binding Non-traceable author statement Ref.3Ref.2. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
FHIT	P49789	4	EBI-711483,EBI-741760

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.10
	Chain	2 – 102	101	10 kDa heat shock protein, mitochondrial		PRO_0000174917
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.10 Ref.13
	Modified residue	56	1	N6-acetyllysine Ref.15
	Modified residue	70	1	N6-acetyllysine Ref.15
	Modified residue	76	1	Phosphotyrosine Ref.14
	Modified residue	86	1	N6-acetyllysine Ref.15
	Modified residue	88	1	Phosphotyrosine Ref.12
	Modified residue	99	1	N6-acetyllysine Ref.15

Sequences

	Sequence		Length	Mass (Da)
	P61604 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 1F3192C81F6EDB78	FASTA	102	10,932
	10 20 30 40 50 60 MAGQAFRKFL PLFDRVLVER SAAETVTKGG IMLPEKSQGK VLQATVVAVG SGSKGKGGEI 70 80 90 100 QPVSVKVGDK VLLPEYGGTK VVLDDKDYFL FRDGDILGKY VD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and cloning of human chaperonin 10 homologue." Monzini N., Legname G., Marcucci F., Gromo G., Modena D. Biochim. Biophys. Acta 1218:478-480(1994) [PubMed: 7914093] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Isolation, sequence analysis and characterization of a cDNA encoding human chaperonin 10." Chen J.J., McNealy D.J., Dalal S., Androphy E.J. Biochim. Biophys. Acta 1219:189-190(1994) [PubMed: 7916212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Genomic structure of the human mitochondrial chaperonin genes: HSP60 and HSP10 are localised head to head on chromosome 2 separated by a bidirectional promoter." Hansen J.J., Bross P., Westergaard M., Nielsen M.N., Eiberg H., Boerglum A.D., Mogensen J., Kristiansen K., Bolund L., Gregersen N. Hum. Genet. 112:71-77(2003) [PubMed: 12483302] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus.
[9]	"Mapping and characterization of the eukaryotic early pregnancy factor/chaperonin 10 gene family." Summers K.M., Fletcher B.H., Macaranas D.D., Somodevilla-Torres M.J., Murphy R.M., Osborne M.J., Spurr N.K., Cassady A.I., Cavanagh A.C. Somat. Cell Mol. Genet. 24:315-326(1998) [PubMed: 10763410] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-100.
[10]	Bienvenut W.V. Submitted (MAR-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-15; 41-54; 57-66 AND 81-92, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Tissue: B-cell lymphoma.
[11]	"The purification of early-pregnancy factor to homogeneity from human platelets and identification as chaperonin 10." Cavanagh A.C., Morton H. Eur. J. Biochem. 222:551-560(1994) [PubMed: 7912672] [Abstract] Cited for: PROTEIN SEQUENCE OF 8-19; 28-54 AND 70-102, MASS SPECTROMETRY. Tissue: Platelet.
[12]	"Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. , Yuan J., Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X., Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J. Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-88, MASS SPECTROMETRY. Tissue: Lung carcinoma.
[13]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[14]	"An extensive survey of tyrosine phosphorylation revealing new sites in human mammary epithelial cells." Heibeck T.H., Ding S.-J., Opresko L.K., Zhao R., Schepmoes A.A., Yang F., Tolmachev A.V., Monroe M.E., Camp D.G. II, Smith R.D., Wiley H.S., Qian W.-J. J. Proteome Res. 8:3852-3861(2009) [PubMed: 19534553] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-76, MASS SPECTROMETRY. Tissue: Mammary epithelium.
[15]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-56; LYS-70; LYS-86 AND LYS-99, MASS SPECTROMETRY.
[16]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X75821 mRNA. Translation: CAA53455.1. U07550 mRNA. Translation: AAA50953.1. AJ250915 Genomic DNA. Translation: CAB75425.1. AK312104 mRNA. Translation: BAG35040.1. CR407688 mRNA. Translation: CAG28616.1. AC020550 Genomic DNA. Translation: AAX93146.1. CH471063 Genomic DNA. Translation: EAW70163.1. BC023518 mRNA. Translation: AAH23518.1. AF107894, AF107893 Genomic DNA. Translation: AAC95387.1.
IPI	IPI00220362.
PIR	S47532.
RefSeq	NP_002148.1. NM_002157.2.
UniGene	Hs.1197.
3D structure databases
ProteinModelPortal	P61604.
SMR	P61604. Positions 7-100.
ModBase	Search...
Protein-protein interaction databases
IntAct	P61604. 22 interactions.
MINT	MINT-1373857.
STRING	P61604.
PTM databases
PhosphoSite	P61604.
Polymorphism databases
DMDM	47606335.
2D gel databases
UCD-2DPAGE	P61604.
Proteomic databases
PeptideAtlas	P61604.
PRIDE	P61604.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000233893; ENSP00000233893; ENSG00000115541.
GeneID	3336.
KEGG	hsa:3336.
UCSC	uc002uul.1. human.
Organism-specific databases
CTD	3336.
GeneCards	GC02P198328.
H-InvDB	HIX0200330.
HGNC	HGNC:5269. HSPE1.
HPA	CAB017366.
MIM	600141. gene.
neXtProt	NX_P61604.
PharmGKB	PA29535.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG21316.
HOGENOM	HBG703377.
HOVERGEN	HBG000385.
InParanoid	P61604.
OMA	DKEYSIF.
OrthoDB	EOG45DWR5.
PhylomeDB	P61604.
Gene expression databases
ArrayExpress	P61604.
Bgee	P61604.
CleanEx	HS_HSPE1.
Genevestigator	P61604.
GermOnline	ENSG00000115541. Homo sapiens.
Family and domain databases
InterPro	IPR020818. Chaperonin_Cpn10. IPR018369. Chaprnonin_Cpn10_CS. IPR011032. GroES-like. [Graphical view]
Gene3D	G3DSA:2.30.33.40. Chaprnin_Cpn10. 1 hit.
KO	K04078.
PANTHER	PTHR10772. Chaprnin_Cpn10. 1 hit.
Pfam	PF00166. Cpn10. 1 hit. [Graphical view]
PRINTS	PR00297. CHAPERONIN10.
SMART	SM00883. Cpn10. 1 hit. [Graphical view]
SUPFAM	SSF50129. GroES_like. 1 hit.
PROSITE	PS00681. CHAPERONINS_CPN10. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	13204.
SOURCE	Search...

Entry information

Entry name

CH10_HUMAN

Accession

Primary (citable) accession number: P61604
Secondary accession number(s): O95421 , Q04984, Q53X54, Q9UDH0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.