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P61604 (CH10_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
10 kDa heat shock protein, mitochondrial

Short name=Hsp10
Alternative name(s):
10 kDa chaperonin
Chaperonin 10
Short name=CPN10
Early-pregnancy factor
Short name=EPF
Gene names
Name:HSPE1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length102 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. HAMAP-Rule MF_00580

Subunit structure

Homohexamer.

Subcellular location

Mitochondrion matrix HAMAP-Rule MF_00580.

Induction

By stress. HAMAP-Rule MF_00580

Sequence similarities

Belongs to the GroES chaperonin family.

Mass spectrometry

Molecular mass is 10843.5±0.2 Da from positions 2 - 102. Determined by ESI. Ref.11

Binary interactions

With

Entry

#Exp.

IntAct

Notes

FHITP497894EBI-711483,EBI-741760

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.10
Chain2 – 10210110 kDa heat shock protein, mitochondrial HAMAP-Rule MF_00580
PRO_0000174917

Amino acid modifications

Modified residue21N-acetylalanine Ref.10
Modified residue81N6-acetyllysine By similarity
Modified residue281N6-succinyllysine By similarity
Modified residue401N6-acetyllysine; alternate By similarity
Modified residue401N6-malonyllysine; alternate Ref.15
Modified residue401N6-succinyllysine; alternate By similarity
Modified residue541N6-malonyllysine; alternate Ref.15
Modified residue541N6-succinyllysine; alternate By similarity
Modified residue561N6-acetyllysine; alternate Ref.12
Modified residue561N6-malonyllysine; alternate Ref.15
Modified residue561N6-succinyllysine; alternate By similarity
Modified residue661N6-acetyllysine; alternate By similarity
Modified residue661N6-succinyllysine; alternate By similarity
Modified residue701N6-acetyllysine; alternate By similarity
Modified residue701N6-succinyllysine; alternate By similarity
Modified residue791Phosphothreonine Ref.13
Modified residue801N6-acetyllysine; alternate By similarity
Modified residue801N6-succinyllysine; alternate By similarity
Modified residue861N6-acetyllysine; alternate Ref.12
Modified residue861N6-succinyllysine; alternate By similarity
Modified residue991N6-acetyllysine Ref.12

Sequences

Sequence LengthMass (Da)Tools
P61604 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 1F3192C81F6EDB78

FASTA10210,932
        10         20         30         40         50         60 
MAGQAFRKFL PLFDRVLVER SAAETVTKGG IMLPEKSQGK VLQATVVAVG SGSKGKGGEI 

        70         80         90        100 
QPVSVKVGDK VLLPEYGGTK VVLDDKDYFL FRDGDILGKY VD 

« Hide

References

« Hide 'large scale' references
[1]"Identification and cloning of human chaperonin 10 homologue."
Monzini N., Legname G., Marcucci F., Gromo G., Modena D.
Biochim. Biophys. Acta 1218:478-480(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Isolation, sequence analysis and characterization of a cDNA encoding human chaperonin 10."
Chen J.J., McNealy D.J., Dalal S., Androphy E.J.
Biochim. Biophys. Acta 1219:189-190(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Genomic structure of the human mitochondrial chaperonin genes: HSP60 and HSP10 are localised head to head on chromosome 2 separated by a bidirectional promoter."
Hansen J.J., Bross P., Westergaard M., Nielsen M.N., Eiberg H., Boerglum A.D., Mogensen J., Kristiansen K., Bolund L., Gregersen N.
Hum. Genet. 112:71-77(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[5]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Uterus.
[9]"Mapping and characterization of the eukaryotic early pregnancy factor/chaperonin 10 gene family."
Summers K.M., Fletcher B.H., Macaranas D.D., Somodevilla-Torres M.J., Murphy R.M., Osborne M.J., Spurr N.K., Cassady A.I., Cavanagh A.C.
Somat. Cell Mol. Genet. 24:315-326(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-100.
[10]Bienvenut W.V.
Submitted (MAR-2005) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 2-15; 41-54; 57-66 AND 81-92, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: B-cell lymphoma.
[11]"The purification of early-pregnancy factor to homogeneity from human platelets and identification as chaperonin 10."
Cavanagh A.C., Morton H.
Eur. J. Biochem. 222:551-560(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 8-19; 28-54 AND 70-102, MASS SPECTROMETRY.
Tissue: Platelet.
[12]"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-56; LYS-86 AND LYS-99, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-79, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[14]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[15]"The first identification of lysine malonylation substrates and its regulatory enzyme."
Peng C., Lu Z., Xie Z., Cheng Z., Chen Y., Tan M., Luo H., Zhang Y., He W., Yang K., Zwaans B.M., Tishkoff D., Ho L., Lombard D., He T.C., Dai J., Verdin E., Ye Y., Zhao Y.
Mol. Cell. Proteomics 10:M111.012658.01-M111.012658.12(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: MALONYLATION AT LYS-40; LYS-54 AND LYS-56.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X75821 mRNA. Translation: CAA53455.1.
U07550 mRNA. Translation: AAA50953.1.
AJ250915 Genomic DNA. Translation: CAB75425.1.
AK312104 mRNA. Translation: BAG35040.1.
CR407688 mRNA. Translation: CAG28616.1.
AC020550 Genomic DNA. Translation: AAX93146.1.
CH471063 Genomic DNA. Translation: EAW70163.1.
BC023518 mRNA. Translation: AAH23518.1.
AH007060 Genomic DNA. Translation: AAC95387.1.
PIRS47532.
RefSeqNP_002148.1. NM_002157.2.
UniGeneHs.1197.

3D structure databases

ProteinModelPortalP61604.
SMRP61604. Positions 11-98.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109568. 36 interactions.
IntActP61604. 25 interactions.
MINTMINT-1373857.
STRING9606.ENSP00000233893.

PTM databases

PhosphoSiteP61604.

Polymorphism databases

DMDM47606335.

2D gel databases

UCD-2DPAGEP61604.

Proteomic databases

PaxDbP61604.
PeptideAtlasP61604.
PRIDEP61604.

Protocols and materials databases

DNASU3336.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000233893; ENSP00000233893; ENSG00000115541.
GeneID3336.
KEGGhsa:3336.
UCSCuc002uul.3. human.

Organism-specific databases

CTD3336.
GeneCardsGC02P198328.
HGNCHGNC:5269. HSPE1.
HPACAB017366.
HPA038755.
MIM600141. gene.
neXtProtNX_P61604.
PharmGKBPA29535.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0234.
HOGENOMHOG000133897.
HOVERGENHBG000385.
InParanoidP61604.
KOK04078.
OMALYIPDTG.
OrthoDBEOG71CFPD.
PhylomeDBP61604.
TreeFamTF313814.

Gene expression databases

ArrayExpressP61604.
BgeeP61604.
CleanExHS_HSPE1.
GenevestigatorP61604.

Family and domain databases

Gene3D2.30.33.40. 1 hit.
HAMAPMF_00580. CH10.
InterProIPR020818. Chaperonin_Cpn10.
IPR018369. Chaprnonin_Cpn10_CS.
IPR011032. GroES-like.
[Graphical view]
PANTHERPTHR10772. PTHR10772. 1 hit.
PfamPF00166. Cpn10. 1 hit.
[Graphical view]
PRINTSPR00297. CHAPERONIN10.
SMARTSM00883. Cpn10. 1 hit.
[Graphical view]
SUPFAMSSF50129. SSF50129. 1 hit.
PROSITEPS00681. CHAPERONINS_CPN10. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiGroES.
GenomeRNAi3336.
NextBio13204.
PROP61604.
SOURCESearch...

Entry information

Entry nameCH10_HUMAN
AccessionPrimary (citable) accession number: P61604
Secondary accession number(s): O95421 expand/collapse secondary AC list , Q04984, Q53X54, Q9UDH0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM