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P61604

- CH10_HUMAN

UniProt

P61604 - CH10_HUMAN

Protein

10 kDa heat shock protein, mitochondrial

Gene

HSPE1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.

    GO - Molecular functioni

    1. ATP binding Source: InterPro
    2. chaperone binding Source: UniProtKB
    3. poly(A) RNA binding Source: UniProtKB
    4. protein binding Source: UniProtKB
    5. unfolded protein binding Source: UniProtKB

    GO - Biological processi

    1. activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
    2. osteoblast differentiation Source: UniProt
    3. protein folding Source: ProtInc
    4. response to unfolded protein Source: ProtInc

    Keywords - Molecular functioni

    Chaperone

    Keywords - Biological processi

    Stress response

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    10 kDa heat shock protein, mitochondrial
    Short name:
    Hsp10
    Alternative name(s):
    10 kDa chaperonin
    Chaperonin 10
    Short name:
    CPN10
    Early-pregnancy factor
    Short name:
    EPF
    Gene namesi
    Name:HSPE1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:5269. HSPE1.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular vesicular exosome Source: UniProt
    2. membrane Source: UniProt
    3. mitochondrial matrix Source: UniProtKB-SubCell
    4. mitochondrion Source: UniProtKB

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA29535.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 10210110 kDa heat shock protein, mitochondrialPRO_0000174917Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication
    Modified residuei8 – 81N6-acetyllysineBy similarity
    Modified residuei28 – 281N6-succinyllysineBy similarity
    Modified residuei40 – 401N6-acetyllysine; alternateBy similarity
    Modified residuei40 – 401N6-malonyllysine; alternate1 Publication
    Modified residuei40 – 401N6-succinyllysine; alternateBy similarity
    Modified residuei54 – 541N6-malonyllysine; alternate1 Publication
    Modified residuei54 – 541N6-succinyllysine; alternateBy similarity
    Modified residuei56 – 561N6-acetyllysine; alternate1 Publication
    Modified residuei56 – 561N6-malonyllysine; alternate1 Publication
    Modified residuei56 – 561N6-succinyllysine; alternateBy similarity
    Modified residuei66 – 661N6-acetyllysine; alternateBy similarity
    Modified residuei66 – 661N6-succinyllysine; alternateBy similarity
    Modified residuei70 – 701N6-acetyllysine; alternateBy similarity
    Modified residuei70 – 701N6-succinyllysine; alternateBy similarity
    Modified residuei79 – 791Phosphothreonine1 Publication
    Modified residuei80 – 801N6-acetyllysine; alternateBy similarity
    Modified residuei80 – 801N6-succinyllysine; alternateBy similarity
    Modified residuei86 – 861N6-acetyllysine; alternate1 Publication
    Modified residuei86 – 861N6-succinyllysine; alternateBy similarity
    Modified residuei99 – 991N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP61604.
    PaxDbiP61604.
    PeptideAtlasiP61604.
    PRIDEiP61604.

    2D gel databases

    UCD-2DPAGEP61604.

    PTM databases

    PhosphoSiteiP61604.

    Expressioni

    Inductioni

    By stress.

    Gene expression databases

    ArrayExpressiP61604.
    BgeeiP61604.
    CleanExiHS_HSPE1.
    GenevestigatoriP61604.

    Organism-specific databases

    HPAiCAB017366.
    HPA038755.

    Interactioni

    Subunit structurei

    Homohexamer.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    FHITP497894EBI-711483,EBI-741760

    Protein-protein interaction databases

    BioGridi109568. 37 interactions.
    IntActiP61604. 25 interactions.
    MINTiMINT-1373857.
    STRINGi9606.ENSP00000233893.

    Structurei

    3D structure databases

    ProteinModelPortaliP61604.
    SMRiP61604. Positions 11-98.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GroES chaperonin family.Curated

    Phylogenomic databases

    eggNOGiCOG0234.
    HOGENOMiHOG000133897.
    HOVERGENiHBG000385.
    InParanoidiP61604.
    KOiK04078.
    OMAiEEYQLFR.
    OrthoDBiEOG71CFPD.
    PhylomeDBiP61604.
    TreeFamiTF313814.

    Family and domain databases

    Gene3Di2.30.33.40. 1 hit.
    HAMAPiMF_00580. CH10.
    InterProiIPR020818. Chaperonin_Cpn10.
    IPR018369. Chaprnonin_Cpn10_CS.
    IPR011032. GroES-like.
    [Graphical view]
    PANTHERiPTHR10772. PTHR10772. 1 hit.
    PfamiPF00166. Cpn10. 1 hit.
    [Graphical view]
    PRINTSiPR00297. CHAPERONIN10.
    SMARTiSM00883. Cpn10. 1 hit.
    [Graphical view]
    SUPFAMiSSF50129. SSF50129. 1 hit.
    PROSITEiPS00681. CHAPERONINS_CPN10. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P61604-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGQAFRKFL PLFDRVLVER SAAETVTKGG IMLPEKSQGK VLQATVVAVG    50
    SGSKGKGGEI QPVSVKVGDK VLLPEYGGTK VVLDDKDYFL FRDGDILGKY 100
    VD 102
    Length:102
    Mass (Da):10,932
    Last modified:January 23, 2007 - v2
    Checksum:i1F3192C81F6EDB78
    GO

    Mass spectrometryi

    Molecular mass is 10843.5±0.2 Da from positions 2 - 102. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75821 mRNA. Translation: CAA53455.1.
    U07550 mRNA. Translation: AAA50953.1.
    AJ250915 Genomic DNA. Translation: CAB75425.1.
    AK312104 mRNA. Translation: BAG35040.1.
    CR407688 mRNA. Translation: CAG28616.1.
    AC020550 Genomic DNA. Translation: AAX93146.1.
    CH471063 Genomic DNA. Translation: EAW70163.1.
    BC023518 mRNA. Translation: AAH23518.1.
    AH007060 Genomic DNA. Translation: AAC95387.1.
    CCDSiCCDS2320.1.
    PIRiS47532.
    RefSeqiNP_002148.1. NM_002157.2.
    UniGeneiHs.1197.

    Genome annotation databases

    EnsembliENST00000233893; ENSP00000233893; ENSG00000115541.
    GeneIDi3336.
    KEGGihsa:3336.
    UCSCiuc002uul.3. human.

    Polymorphism databases

    DMDMi47606335.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75821 mRNA. Translation: CAA53455.1 .
    U07550 mRNA. Translation: AAA50953.1 .
    AJ250915 Genomic DNA. Translation: CAB75425.1 .
    AK312104 mRNA. Translation: BAG35040.1 .
    CR407688 mRNA. Translation: CAG28616.1 .
    AC020550 Genomic DNA. Translation: AAX93146.1 .
    CH471063 Genomic DNA. Translation: EAW70163.1 .
    BC023518 mRNA. Translation: AAH23518.1 .
    AH007060 Genomic DNA. Translation: AAC95387.1 .
    CCDSi CCDS2320.1.
    PIRi S47532.
    RefSeqi NP_002148.1. NM_002157.2.
    UniGenei Hs.1197.

    3D structure databases

    ProteinModelPortali P61604.
    SMRi P61604. Positions 11-98.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 109568. 37 interactions.
    IntActi P61604. 25 interactions.
    MINTi MINT-1373857.
    STRINGi 9606.ENSP00000233893.

    PTM databases

    PhosphoSitei P61604.

    Polymorphism databases

    DMDMi 47606335.

    2D gel databases

    UCD-2DPAGE P61604.

    Proteomic databases

    MaxQBi P61604.
    PaxDbi P61604.
    PeptideAtlasi P61604.
    PRIDEi P61604.

    Protocols and materials databases

    DNASUi 3336.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000233893 ; ENSP00000233893 ; ENSG00000115541 .
    GeneIDi 3336.
    KEGGi hsa:3336.
    UCSCi uc002uul.3. human.

    Organism-specific databases

    CTDi 3336.
    GeneCardsi GC02P198328.
    HGNCi HGNC:5269. HSPE1.
    HPAi CAB017366.
    HPA038755.
    MIMi 600141. gene.
    neXtProti NX_P61604.
    PharmGKBi PA29535.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0234.
    HOGENOMi HOG000133897.
    HOVERGENi HBG000385.
    InParanoidi P61604.
    KOi K04078.
    OMAi EEYQLFR.
    OrthoDBi EOG71CFPD.
    PhylomeDBi P61604.
    TreeFami TF313814.

    Miscellaneous databases

    GeneWikii GroES.
    GenomeRNAii 3336.
    NextBioi 13204.
    PROi P61604.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P61604.
    Bgeei P61604.
    CleanExi HS_HSPE1.
    Genevestigatori P61604.

    Family and domain databases

    Gene3Di 2.30.33.40. 1 hit.
    HAMAPi MF_00580. CH10.
    InterProi IPR020818. Chaperonin_Cpn10.
    IPR018369. Chaprnonin_Cpn10_CS.
    IPR011032. GroES-like.
    [Graphical view ]
    PANTHERi PTHR10772. PTHR10772. 1 hit.
    Pfami PF00166. Cpn10. 1 hit.
    [Graphical view ]
    PRINTSi PR00297. CHAPERONIN10.
    SMARTi SM00883. Cpn10. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50129. SSF50129. 1 hit.
    PROSITEi PS00681. CHAPERONINS_CPN10. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and cloning of human chaperonin 10 homologue."
      Monzini N., Legname G., Marcucci F., Gromo G., Modena D.
      Biochim. Biophys. Acta 1218:478-480(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Isolation, sequence analysis and characterization of a cDNA encoding human chaperonin 10."
      Chen J.J., McNealy D.J., Dalal S., Androphy E.J.
      Biochim. Biophys. Acta 1219:189-190(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Genomic structure of the human mitochondrial chaperonin genes: HSP60 and HSP10 are localised head to head on chromosome 2 separated by a bidirectional promoter."
      Hansen J.J., Bross P., Westergaard M., Nielsen M.N., Eiberg H., Boerglum A.D., Mogensen J., Kristiansen K., Bolund L., Gregersen N.
      Hum. Genet. 112:71-77(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    5. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Uterus.
    9. "Mapping and characterization of the eukaryotic early pregnancy factor/chaperonin 10 gene family."
      Summers K.M., Fletcher B.H., Macaranas D.D., Somodevilla-Torres M.J., Murphy R.M., Osborne M.J., Spurr N.K., Cassady A.I., Cavanagh A.C.
      Somat. Cell Mol. Genet. 24:315-326(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-100.
    10. Bienvenut W.V.
      Submitted (MAR-2005) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 2-15; 41-54; 57-66 AND 81-92, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: B-cell lymphoma.
    11. "The purification of early-pregnancy factor to homogeneity from human platelets and identification as chaperonin 10."
      Cavanagh A.C., Morton H.
      Eur. J. Biochem. 222:551-560(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 8-19; 28-54 AND 70-102, MASS SPECTROMETRY.
      Tissue: Platelet.
    12. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
      Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
      Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-56; LYS-86 AND LYS-99, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-79, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    14. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    15. Cited for: MALONYLATION AT LYS-40; LYS-54 AND LYS-56.

    Entry informationi

    Entry nameiCH10_HUMAN
    AccessioniPrimary (citable) accession number: P61604
    Secondary accession number(s): O95421
    , Q04984, Q53X54, Q9UDH0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 24, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3