Neurocalcin-delta - Bos taurus (Bovine)

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Reviewed, UniProtKB/Swiss-Prot P61602 (NCALD_BOVIN)

Last modified May 5, 2009. Version 49. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Neurocalcin-delta

Gene names

Name:

NCALD

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

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Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	May be involved in the calcium-dependent regulation of rhodopsin phosphorylation. Binds three calcium ions.
Tissue specificity	Retina, cerebrum, cerebellum, brain stem, spinal cord, testis, ovary and small intestine.
Miscellaneous	Five isoprotein forms of neurocalcin are designated alpha, beta, gamma1, gamma2 and delta.
Sequence similarities	Belongs to the recoverin family. Contains 4 EF-hand domains.

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Ontologies

Keywords
Domain	Repeat
Ligand	Calcium
PTM	Lipoprotein Myristate
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular function	actin binding Inferred from sequence or structural similarity. Source: UniProtKB calcium ion binding Ref.2 Non-traceable author statement. Source: UniProtKB clathrin binding Inferred from sequence or structural similarity. Source: UniProtKB tubulin binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
PDE1A	P14100	1	EBI-908133,EBI-907809

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed

Chain

2 – 193

192

Neurocalcin-delta

PRO_0000073781

Regions

Domain

23 – 58

EF-hand 1

Domain

60 – 95

EF-hand 2

Domain

96 – 131

EF-hand 3

Domain

144 – 179

EF-hand 4

Calcium binding

73 – 84

1 Potential

Calcium binding

109 – 120

2 Potential

Calcium binding

157 – 168

3 Potential

Amino acid modifications

Lipidation

N-myristoyl glycine

Secondary structure

193

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P61602-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 20DD8B6FF47C4CBF
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FASTA

193

22,245

        10         20         30         40         50         60 
MGKQNSKLRP EVMQDLLEST DFTEHEIQEW YKGFLRDCPS GHLSMEEFKK IYGNFFPYGD 

        70         80         90        100        110        120 
ASKFAEHVFR TFDANGDGTI DFREFIIALS VTSRGKLEQK LKWAFSMYDL DGNGYISKAE 

       130        140        150        160        170        180 
MLEIVQAIYK MVSSVMKMPE DESTPEKRTE KIFRQMDTNR DGKLSLEEFI RGAKSDPSIV 

       190 
RLLQCDPSSA GQF

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Full sequence of neurocalcin, a novel calcium-binding protein abundant in central nervous system." Okazaki K., Watanabe M., Ando Y., Hagiwara M., Terasawa M., Hidaka H. Biochem. Biophys. Res. Commun. 185:147-153(1992) [PubMed: 1599450] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 107-112 AND 121-126. Tissue: Brain.
[2]	"Neurocalcin family: a novel calcium-binding protein abundant in bovine central nervous system." Hidaka H., Okazaki K. Neurosci. Res. 16:73-77(1993) [PubMed: 8387172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Thalamus.
[5]	"Calcium and membrane binding properties of bovine neurocalcin delta expressed in Escherichia coli." Ladant D. J. Biol. Chem. 270:3179-3185(1995) [PubMed: 7852401] [Abstract] Cited for: CALCIUM-BINDING DATA.
[6]	"Crystallization and preliminary X-ray crystallographic studies of recombinant bovine neurocalcin delta." Kumar V.D., Hidaka H., Okazaki K., Vijay-Kumar S. Proteins 25:261-264(1996) [PubMed: 8811741] [Abstract] Cited for: CRYSTALLIZATION.
[7]	"Crystal structure of recombinant bovine neurocalcin." Vijay-Kumar S., Kumar V.D. Nat. Struct. Biol. 6:80-88(1999) [PubMed: 9886296] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).

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Cross-references

Sequence databases

D10884 mRNA. Translation: BAA01706.1.
BT029820 mRNA. Translation: ABM06083.1.
BC122588 mRNA. Translation: AAI22589.1.

IPI

IPI00698069.

PIR

JH0616.

RefSeq

NP_776823.1.

UniGene

Bt.65801

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BJF	X-ray	2.40	A/B	1-193	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P61602. 2 interactions.

Genome annotation databases

Ensembl

ENSBTAG00000004259. Bos taurus. [Contig view]

GeneID

281940.

KEGG

bta:281940.

Phylogenomic databases

HOVERGEN

P61602.

Family and domain databases

InterPro

IPR011992. EF-Hand_type.
IPR018248. EF_hand.
IPR018247. EF_HAND_1.
IPR018249. EF_HAND_2.
IPR002048. EF_hand_Ca_bd.
IPR001125. Recoverin.
[Graphical view]

Gene3D

G3DSA:1.10.238.10. EF-Hand_type. 1 hit.

Pfam

PF00036. efhand. 3 hits.
[Graphical view]

PRINTS

PR00450. RECOVERIN.

ProDom

PD000012. EF-hand. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00054. EFh. 3 hits.
[Graphical view]

PROSITE

PS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

NCALD_BOVIN

Accession

Primary (citable) accession number: P61602
Secondary accession number(s): A1L533

Q9H0W2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	January 23, 2007
Last modified:	May 5, 2009
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents