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P61589

- RHOA_RAT

UniProt

P61589 - RHOA_RAT

Protein

Transforming protein RhoA

Gene

Rhoa

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (24 May 2004)
      Previous versions | rss
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    Functioni

    Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization By similarity.By similarity

    Enzyme regulationi

    GTP hydrolysis is stimulated by ARHGAP30.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi12 – 198GTPBy similarity
    Nucleotide bindingi59 – 635GTPBy similarity
    Nucleotide bindingi117 – 1204GTPBy similarity

    GO - Molecular functioni

    1. GDP binding Source: RGD
    2. GTPase activity Source: BHF-UCL
    3. GTP binding Source: RGD
    4. protein binding Source: RGD
    5. protein domain specific binding Source: RGD

    GO - Biological processi

    1. actin cytoskeleton organization Source: RGD
    2. androgen receptor signaling pathway Source: Ensembl
    3. apical junction assembly Source: UniProtKB
    4. cell-matrix adhesion Source: Ensembl
    5. cellular response to progesterone stimulus Source: BHF-UCL
    6. cerebral cortex cell migration Source: Ensembl
    7. cleavage furrow formation Source: UniProtKB
    8. forebrain radial glial cell differentiation Source: Ensembl
    9. GTP catabolic process Source: BHF-UCL
    10. negative regulation of cell death Source: RGD
    11. negative regulation of I-kappaB kinase/NF-kappaB signaling Source: RGD
    12. negative regulation of intracellular steroid hormone receptor signaling pathway Source: Ensembl
    13. negative regulation of neuron apoptotic process Source: Ensembl
    14. negative regulation of neuron differentiation Source: RGD
    15. neuron projection morphogenesis Source: RGD
    16. ossification involved in bone maturation Source: Ensembl
    17. positive regulation of actin filament polymerization Source: RGD
    18. positive regulation of cell adhesion Source: RGD
    19. positive regulation of cell growth Source: RGD
    20. positive regulation of cell migration Source: RGD
    21. positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: RGD
    22. positive regulation of cytokinesis Source: UniProtKB
    23. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
    24. positive regulation of neuron apoptotic process Source: RGD
    25. positive regulation of podosome assembly Source: Ensembl
    26. positive regulation of smooth muscle contraction Source: RGD
    27. positive regulation of translation Source: RGD
    28. positive regulation of vasoconstriction Source: RGD
    29. regulation of actin polymerization or depolymerization Source: RGD
    30. regulation of calcium ion transport Source: RGD
    31. regulation of cell migration Source: UniProtKB
    32. regulation of dendrite development Source: RGD
    33. regulation of neural precursor cell proliferation Source: Ensembl
    34. regulation of osteoblast proliferation Source: Ensembl
    35. regulation of transcription from RNA polymerase II promoter Source: Ensembl
    36. response to amino acid Source: RGD
    37. response to drug Source: RGD
    38. response to ethanol Source: RGD
    39. response to glucocorticoid Source: RGD
    40. response to glucose Source: RGD
    41. response to hypoxia Source: RGD
    42. response to mechanical stimulus Source: RGD
    43. skeletal muscle tissue development Source: Ensembl
    44. small GTPase mediated signal transduction Source: InterPro
    45. stress-activated protein kinase signaling cascade Source: RGD
    46. stress fiber assembly Source: Ensembl
    47. trabecula morphogenesis Source: Ensembl

    Keywords - Biological processi

    Cell cycle, Cell division

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_197601. Axonal growth stimulation.
    REACT_198389. PCP/CE pathway.
    REACT_198828. TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transforming protein RhoA
    Gene namesi
    Name:Rhoa
    Synonyms:Arha, Arha2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 8

    Organism-specific databases

    RGDi619921. Rhoa.

    Subcellular locationi

    Cell membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity. Cytoplasmcytoskeleton By similarity. Cleavage furrow By similarity. Cytoplasmcell cortex By similarity. Midbody By similarity. Cell projectionlamellipodium By similarity
    Note: Translocates to the equatorial region before furrow formation in a ECT2-dependent manner. Localizes to the equatorial cell cortex (at the site of the presumptive furrow) in early anaphase in a activated form and in a myosin- and actin-independent manner. Localized to cell-cell contacts in calcium-treated keratinocytes By similarity.By similarity

    GO - Cellular componenti

    1. apical junction complex Source: UniProtKB
    2. axon Source: RGD
    3. cell cortex Source: UniProtKB
    4. cleavage furrow Source: UniProtKB-SubCell
    5. cytoplasm Source: RGD
    6. cytoskeleton Source: UniProtKB-SubCell
    7. cytosol Source: BHF-UCL
    8. lamellipodium Source: UniProtKB
    9. membrane Source: BHF-UCL
    10. midbody Source: UniProtKB-SubCell
    11. mitochondrion Source: Ensembl
    12. nucleus Source: Ensembl
    13. ruffle membrane Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

    Pathology & Biotechi

    Keywords - Diseasei

    Proto-oncogene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 190190Transforming protein RhoAPRO_0000030415Add
    BLAST
    Propeptidei191 – 1933Removed in mature formBy similarityPRO_0000030416

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei188 – 1881Phosphoserine; by PKG/PRKG1By similarity
    Modified residuei190 – 1901Cysteine methyl esterBy similarity
    Lipidationi190 – 1901S-geranylgeranyl cysteineBy similarity

    Post-translational modificationi

    Substrate for botulinum ADP-ribosyltransferase.By similarity
    Phosphorylation by PRKG1 at Ser-188 inactivates RHOA signaling.By similarity
    Ubiquitinated by the BCR(BACURD1) and BCR(BACURD2) E3 ubiquitin ligase complexes, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration.By similarity

    Keywords - PTMi

    Lipoprotein, Methylation, Phosphoprotein, Prenylation, Ubl conjugation

    Proteomic databases

    PaxDbiP61589.
    PRIDEiP61589.

    2D gel databases

    World-2DPAGE0004:P61589.

    PTM databases

    PhosphoSiteiP61589.

    Expressioni

    Gene expression databases

    ArrayExpressiP61589.
    GenevestigatoriP61589.

    Interactioni

    Subunit structurei

    Binds PRKCL1, ROCK1 and ROCK2. Interacts with ARHGEF2, ARHGEF3, NET1 and RTKN. Interacts with PLCE1 and AKAP13. Interacts with ARHGEF28. Interacts with DIAPH1. Interacts (in the constitutively activated, GTP-bound form) with DGKQ. Interacts with GNB2L1/RACK1; enhances RHOA activation. Interacts with PKP4; the interaction is detected at the midbody. Interacts (GTP-bound form preferentially) with PKN2; the interaction stimulates autophosphorylation and phosphorylation of PKN2 By similarity. Interacts with ARHGDIA; this interaction inactivates and stabilizes RHOA. Interacts with ARHGDIB By similarity.By similarity

    Protein-protein interaction databases

    BioGridi250720. 1 interaction.
    IntActiP61589. 4 interactions.
    MINTiMINT-4542785.

    Structurei

    Secondary structure

    1
    193
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 1310
    Helixi18 – 2710
    Beta strandi43 – 486
    Beta strandi51 – 588
    Helixi65 – 695
    Helixi70 – 723
    Beta strandi78 – 858
    Helixi89 – 979
    Helixi99 – 1068
    Beta strandi112 – 1176
    Helixi119 – 1213
    Helixi125 – 1339
    Helixi141 – 15111
    Beta strandi154 – 1585
    Turni161 – 1644
    Helixi167 – 17913

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3TVDX-ray2.99A/B1-193[»]
    ProteinModelPortaliP61589.
    SMRiP61589. Positions 2-181.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi34 – 429Effector regionSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi182 – 1876Arg/Lys-rich (basic)

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rho family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    GeneTreeiENSGT00700000104143.
    HOGENOMiHOG000233974.
    HOVERGENiHBG009351.
    InParanoidiP61589.
    KOiK04513.
    OMAiRNDPHTI.
    OrthoDBiEOG73FQPD.
    PhylomeDBiP61589.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003578. Small_GTPase_Rho.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00174. RHO. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51420. RHO. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P61589-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAIRKKLVI VGDGACGKTC LLIVFSKDQF PEVYVPTVFE NYVADIEVDG    50
    KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSIDSPD SLENIPEKWT 100
    PEVKHFCPNV PIILVGNKKD LRNDEHTRRE LAKMKQEPVK PEEGRDMANR 150
    IGAFGYMECS AKTKDGVREV FEMATRAALQ ARRGKKKSGC LIL 193
    Length:193
    Mass (Da):21,782
    Last modified:May 24, 2004 - v1
    Checksum:iC4C8BDC31FF858BC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY026068 mRNA. Translation: AAK11717.1.
    AY026069 mRNA. Translation: AAK11718.1.
    BC061732 mRNA. Translation: AAH61732.1.
    RefSeqiNP_476473.1. NM_057132.3.
    XP_006243761.1. XM_006243699.1.
    XP_006243762.1. XM_006243700.1.
    XP_006243763.1. XM_006243701.1.
    UniGeneiRn.107401.

    Genome annotation databases

    EnsembliENSRNOT00000071664; ENSRNOP00000066672; ENSRNOG00000050519.
    GeneIDi117273.
    KEGGirno:117273.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY026068 mRNA. Translation: AAK11717.1 .
    AY026069 mRNA. Translation: AAK11718.1 .
    BC061732 mRNA. Translation: AAH61732.1 .
    RefSeqi NP_476473.1. NM_057132.3.
    XP_006243761.1. XM_006243699.1.
    XP_006243762.1. XM_006243700.1.
    XP_006243763.1. XM_006243701.1.
    UniGenei Rn.107401.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3TVD X-ray 2.99 A/B 1-193 [» ]
    ProteinModelPortali P61589.
    SMRi P61589. Positions 2-181.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 250720. 1 interaction.
    IntActi P61589. 4 interactions.
    MINTi MINT-4542785.

    PTM databases

    PhosphoSitei P61589.

    2D gel databases

    World-2DPAGE 0004:P61589.

    Proteomic databases

    PaxDbi P61589.
    PRIDEi P61589.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000071664 ; ENSRNOP00000066672 ; ENSRNOG00000050519 .
    GeneIDi 117273.
    KEGGi rno:117273.

    Organism-specific databases

    CTDi 387.
    RGDi 619921. Rhoa.

    Phylogenomic databases

    eggNOGi COG1100.
    GeneTreei ENSGT00700000104143.
    HOGENOMi HOG000233974.
    HOVERGENi HBG009351.
    InParanoidi P61589.
    KOi K04513.
    OMAi RNDPHTI.
    OrthoDBi EOG73FQPD.
    PhylomeDBi P61589.

    Enzyme and pathway databases

    Reactomei REACT_197601. Axonal growth stimulation.
    REACT_198389. PCP/CE pathway.
    REACT_198828. TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition).

    Miscellaneous databases

    NextBioi 620199.

    Gene expression databases

    ArrayExpressi P61589.
    Genevestigatori P61589.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003578. Small_GTPase_Rho.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00174. RHO. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51420. RHO. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of Wistar-Kyoto (WKY) and spontaneously hypertensive rat (SHR) RhoA cDNA."
      Andresen B.T., Jackson E.K., Romero G.G.
      Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: SHR and Wistar Kyoto.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Prostate.

    Entry informationi

    Entry nameiRHOA_RAT
    AccessioniPrimary (citable) accession number: P61589
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 24, 2004
    Last sequence update: May 24, 2004
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3