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Protein

Transforming protein RhoA

Gene

Rhoa

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity). Regulates KCNA2 potassium channel activity by reducing its location at the cell surface in response to CHRM1 activation; promotes KCNA2 endocytosis (PubMed:9635436).By similarity1 Publication

Enzyme regulationi

GTP hydrolysis is stimulated by ARHGAP30.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 19GTPBy similarity8
Nucleotide bindingi59 – 63GTPBy similarity5
Nucleotide bindingi117 – 120GTPBy similarity4

GO - Molecular functioni

  • GDP binding Source: RGD
  • GTPase activity Source: BHF-UCL
  • GTP binding Source: RGD
  • myosin binding Source: Ensembl

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-114604 GPVI-mediated activation cascade
R-RNO-193634 Axonal growth inhibition (RHOA activation)
R-RNO-194840 Rho GTPase cycle
R-RNO-198203 PI3K/AKT activation
R-RNO-209563 Axonal growth stimulation
R-RNO-2173791 TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition)
R-RNO-392451 G beta:gamma signalling through PI3Kgamma
R-RNO-3928662 EPHB-mediated forward signaling
R-RNO-3928663 EPHA-mediated growth cone collapse
R-RNO-4086400 PCP/CE pathway
R-RNO-416482 G alpha (12/13) signalling events
R-RNO-416550 Sema4D mediated inhibition of cell attachment and migration
R-RNO-416572 Sema4D induced cell migration and growth-cone collapse
R-RNO-4420097 VEGFA-VEGFR2 Pathway
R-RNO-5625740 RHO GTPases activate PKNs
R-RNO-5625900 RHO GTPases activate CIT
R-RNO-5625970 RHO GTPases activate KTN1
R-RNO-5627117 RHO GTPases Activate ROCKs
R-RNO-5663220 RHO GTPases Activate Formins
R-RNO-5666185 RHO GTPases Activate Rhotekin and Rhophilins
R-RNO-5689896 Ovarian tumor domain proteases
R-RNO-6785631 ERBB2 Regulates Cell Motility
R-RNO-6798695 Neutrophil degranulation
R-RNO-8849471 PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases
R-RNO-8985586 SLIT2:ROBO1 increases RHOA activity

Names & Taxonomyi

Protein namesi
Recommended name:
Transforming protein RhoA
Gene namesi
Name:Rhoa
Synonyms:Arha, Arha2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi619921 Rhoa

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000304151 – 190Transforming protein RhoAAdd BLAST190
PropeptideiPRO_0000030416191 – 193Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei188Phosphoserine; by PKG/PRKG1 and SLK1 Publication1
Modified residuei190Cysteine methyl esterBy similarity1
Lipidationi190S-geranylgeranyl cysteineBy similarity1

Post-translational modificationi

Phosphorylation by PRKG1 at Ser-188 inactivates RHOA signaling (By similarity). Phosphorylation by SLK at Ser-188 in response to AGTR2 activation (PubMed:18420945).By similarity1 Publication
Ubiquitinated by the BCR(KCTD13) and BCR(TNFAIP1) E3 ubiquitin ligase complexes, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and synaptic transmission in neurons.By similarity

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation, Ubl conjugation

Proteomic databases

PaxDbiP61589
PRIDEiP61589

2D gel databases

World-2DPAGEi0004:P61589

PTM databases

iPTMnetiP61589
PhosphoSitePlusiP61589
SwissPalmiP61589

Expressioni

Gene expression databases

BgeeiENSRNOG00000050519
GenevisibleiP61589 RN

Interactioni

Subunit structurei

Binds PRKCL1, ROCK1 and ROCK2. Interacts with ARHGEF2, ARHGEF3, NET1 and RTKN. Interacts with PLCE1 and AKAP13. Interacts with ARHGEF28. Interacts with DIAPH1. Interacts (in the constitutively activated, GTP-bound form) with DGKQ. Interacts with RACK1; enhances RHOA activation. Interacts with PKP4; the interaction is detected at the midbody. Interacts (GTP-bound form preferentially) with PKN2; the interaction stimulates autophosphorylation and phosphorylation of PKN2 (By similarity). Interacts with ARHGDIA; this interaction inactivates and stabilizes RHOA. Interacts with ARHGDIB (By similarity). Interacts (GTP-bound form) with KCNA2 (via cytoplasmic N-terminal domain) (PubMed:9635436). Interacts (via GTP-bound form) with RIPOR1 (via N-terminus); this interaction links RHOA to STK24 and STK26 kinases (By similarity). Interacts with RIPOR2 (via active GTP- or inactive GDP-bound forms); this interaction is direct, blocks the loading of GTP to RHOA and decreases upon chemokine CCL19 stimulation in primary T lymphocytes (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi250720, 2 interactors
IntActiP61589, 5 interactors
MINTiP61589
STRINGi10116.ENSRNOP00000066672

Structurei

Secondary structure

1193
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 13Combined sources10
Helixi18 – 27Combined sources10
Beta strandi43 – 48Combined sources6
Beta strandi51 – 58Combined sources8
Helixi65 – 69Combined sources5
Helixi70 – 72Combined sources3
Beta strandi78 – 85Combined sources8
Helixi89 – 97Combined sources9
Helixi99 – 106Combined sources8
Beta strandi112 – 117Combined sources6
Helixi119 – 121Combined sources3
Helixi125 – 133Combined sources9
Helixi141 – 151Combined sources11
Beta strandi154 – 158Combined sources5
Turni161 – 164Combined sources4
Helixi167 – 179Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TVDX-ray2.99A/B1-193[»]
ProteinModelPortaliP61589
SMRiP61589
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi34 – 42Effector regionSequence analysis9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi182 – 187Arg/Lys-rich (basic)6

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

eggNOGiKOG0393 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00760000119020
HOGENOMiHOG000233974
HOVERGENiHBG009351
InParanoidiP61589
KOiK04513
OMAiQKIGARH
OrthoDBiEOG091G0QVS
PhylomeDBiP61589

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR003578 Small_GTPase_Rho
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51420 RHO, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61589-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAIRKKLVI VGDGACGKTC LLIVFSKDQF PEVYVPTVFE NYVADIEVDG
60 70 80 90 100
KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSIDSPD SLENIPEKWT
110 120 130 140 150
PEVKHFCPNV PIILVGNKKD LRNDEHTRRE LAKMKQEPVK PEEGRDMANR
160 170 180 190
IGAFGYMECS AKTKDGVREV FEMATRAALQ ARRGKKKSGC LIL
Length:193
Mass (Da):21,782
Last modified:May 24, 2004 - v1
Checksum:iC4C8BDC31FF858BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY026068 mRNA Translation: AAK11717.1
AY026069 mRNA Translation: AAK11718.1
BC061732 mRNA Translation: AAH61732.1
RefSeqiNP_476473.1, NM_057132.3
XP_006243761.1, XM_006243699.1
XP_006243762.1, XM_006243700.1
XP_006243763.1, XM_006243701.1
UniGeneiRn.107401

Genome annotation databases

EnsembliENSRNOT00000071664; ENSRNOP00000066672; ENSRNOG00000050519
GeneIDi117273
KEGGirno:117273

Similar proteinsi

Entry informationi

Entry nameiRHOA_RAT
AccessioniPrimary (citable) accession number: P61589
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 24, 2004
Last modified: May 23, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
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