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Protein

Transforming protein RhoA

Gene

Rhoa

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity). Regulates KCNA2 potassium channel activity by reducing its location at the cell surface in response to CHRM1 activation; promotes KCNA2 endocytosis (PubMed:9635436).By similarity1 Publication

Enzyme regulationi

GTP hydrolysis is stimulated by ARHGAP30.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 19GTPBy similarity8
Nucleotide bindingi59 – 63GTPBy similarity5
Nucleotide bindingi117 – 120GTPBy similarity4

GO - Molecular functioni

  • GDP binding Source: RGD
  • GTPase activity Source: BHF-UCL
  • GTP binding Source: RGD
  • myosin binding Source: RGD
  • protein domain specific binding Source: RGD
  • Rho GDP-dissociation inhibitor binding Source: RGD

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-114604. GPVI-mediated activation cascade.
R-RNO-193634. Axonal growth inhibition (RHOA activation).
R-RNO-194840. Rho GTPase cycle.
R-RNO-198203. PI3K/AKT activation.
R-RNO-209563. Axonal growth stimulation.
R-RNO-2173791. TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition).
R-RNO-392451. G beta:gamma signalling through PI3Kgamma.
R-RNO-3928662. EPHB-mediated forward signaling.
R-RNO-3928663. EPHA-mediated growth cone collapse.
R-RNO-4086400. PCP/CE pathway.
R-RNO-416482. G alpha (12/13) signalling events.
R-RNO-416550. Sema4D mediated inhibition of cell attachment and migration.
R-RNO-416572. Sema4D induced cell migration and growth-cone collapse.
R-RNO-4420097. VEGFA-VEGFR2 Pathway.
R-RNO-5625740. RHO GTPases activate PKNs.
R-RNO-5625900. RHO GTPases activate CIT.
R-RNO-5625970. RHO GTPases activate KTN1.
R-RNO-5627117. RHO GTPases Activate ROCKs.
R-RNO-5663220. RHO GTPases Activate Formins.
R-RNO-5666185. RHO GTPases Activate Rhotekin and Rhophilins.
R-RNO-5689896. Ovarian tumor domain proteases.
R-RNO-6785631. ERBB2 Regulates Cell Motility.
R-RNO-6798695. Neutrophil degranulation.
R-RNO-8849471. PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.
R-RNO-8985586. SLIT2:ROBO1 increases RHOA activity.

Names & Taxonomyi

Protein namesi
Recommended name:
Transforming protein RhoA
Gene namesi
Name:Rhoa
Synonyms:Arha, Arha2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi619921. Rhoa.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000304151 – 190Transforming protein RhoAAdd BLAST190
PropeptideiPRO_0000030416191 – 193Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei188Phosphoserine; by PKG/PRKG1 and SLK1 Publication1
Modified residuei190Cysteine methyl esterBy similarity1
Lipidationi190S-geranylgeranyl cysteineBy similarity1

Post-translational modificationi

Substrate for botulinum ADP-ribosyltransferase.By similarity
Phosphorylation by PRKG1 at Ser-188 inactivates RHOA signaling (By similarity). Phosphorylation by SLK at Ser-188 in response to AGTR2 activation (PubMed:18420945).By similarity1 Publication
Ubiquitinated by the BCR(BACURD1) and BCR(BACURD2) E3 ubiquitin ligase complexes, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration.By similarity

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation, Ubl conjugation

Proteomic databases

PaxDbiP61589.
PRIDEiP61589.

2D gel databases

World-2DPAGEi0004:P61589.

PTM databases

iPTMnetiP61589.
PhosphoSitePlusiP61589.

Expressioni

Gene expression databases

BgeeiENSRNOG00000050519.
GenevisibleiP61589. RN.

Interactioni

Subunit structurei

Binds PRKCL1, ROCK1 and ROCK2. Interacts with ARHGEF2, ARHGEF3, NET1 and RTKN. Interacts with PLCE1 and AKAP13. Interacts with ARHGEF28. Interacts with DIAPH1. Interacts (in the constitutively activated, GTP-bound form) with DGKQ. Interacts with RACK1; enhances RHOA activation. Interacts with PKP4; the interaction is detected at the midbody. Interacts (GTP-bound form preferentially) with PKN2; the interaction stimulates autophosphorylation and phosphorylation of PKN2 (By similarity). Interacts with ARHGDIA; this interaction inactivates and stabilizes RHOA. Interacts with ARHGDIB (By similarity). Interacts (GTP-bound form) with KCNA2 (via cytoplasmic N-terminal domain) (PubMed:9635436). Interacts (via GTP-bound form) with RIPOR1 (via N-terminus); this interaction links RHOA to STK24 and STK26 kinases (By similarity). Interacts with RIPOR2 (via active GTP- or inactive GDP-bound forms); this interaction is direct, blocks the loading of GTP to RHOA and decreases upon chemokine CCL19 stimulation in primary T lymphocytes (By similarity).By similarity1 Publication

GO - Molecular functioni

  • myosin binding Source: RGD
  • protein domain specific binding Source: RGD
  • Rho GDP-dissociation inhibitor binding Source: RGD

Protein-protein interaction databases

BioGridi250720. 2 interactors.
IntActiP61589. 5 interactors.
MINTiMINT-4542785.
STRINGi10116.ENSRNOP00000066672.

Structurei

Secondary structure

1193
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 13Combined sources10
Helixi18 – 27Combined sources10
Beta strandi43 – 48Combined sources6
Beta strandi51 – 58Combined sources8
Helixi65 – 69Combined sources5
Helixi70 – 72Combined sources3
Beta strandi78 – 85Combined sources8
Helixi89 – 97Combined sources9
Helixi99 – 106Combined sources8
Beta strandi112 – 117Combined sources6
Helixi119 – 121Combined sources3
Helixi125 – 133Combined sources9
Helixi141 – 151Combined sources11
Beta strandi154 – 158Combined sources5
Turni161 – 164Combined sources4
Helixi167 – 179Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TVDX-ray2.99A/B1-193[»]
ProteinModelPortaliP61589.
SMRiP61589.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi34 – 42Effector regionSequence analysis9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi182 – 187Arg/Lys-rich (basic)6

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

eggNOGiKOG0393. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00760000119020.
HOGENOMiHOG000233974.
HOVERGENiHBG009351.
InParanoidiP61589.
KOiK04513.
OMAiQKIGARH.
OrthoDBiEOG091G0QVS.
PhylomeDBiP61589.

Family and domain databases

InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003578. Small_GTPase_Rho.
PfamiView protein in Pfam
PF00071. Ras. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiView protein in PROSITE
PS51420. RHO. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61589-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAIRKKLVI VGDGACGKTC LLIVFSKDQF PEVYVPTVFE NYVADIEVDG
60 70 80 90 100
KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSIDSPD SLENIPEKWT
110 120 130 140 150
PEVKHFCPNV PIILVGNKKD LRNDEHTRRE LAKMKQEPVK PEEGRDMANR
160 170 180 190
IGAFGYMECS AKTKDGVREV FEMATRAALQ ARRGKKKSGC LIL
Length:193
Mass (Da):21,782
Last modified:May 24, 2004 - v1
Checksum:iC4C8BDC31FF858BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY026068 mRNA. Translation: AAK11717.1.
AY026069 mRNA. Translation: AAK11718.1.
BC061732 mRNA. Translation: AAH61732.1.
RefSeqiNP_476473.1. NM_057132.3.
XP_006243761.1. XM_006243699.1.
XP_006243762.1. XM_006243700.1.
XP_006243763.1. XM_006243701.1.
UniGeneiRn.107401.

Genome annotation databases

EnsembliENSRNOT00000071664; ENSRNOP00000066672; ENSRNOG00000050519.
GeneIDi117273.
KEGGirno:117273.

Similar proteinsi

Entry informationi

Entry nameiRHOA_RAT
AccessioniPrimary (citable) accession number: P61589
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 24, 2004
Last modified: November 22, 2017
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families