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P61589

- RHOA_RAT

UniProt

P61589 - RHOA_RAT

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Protein
Transforming protein RhoA
Gene
Rhoa, Arha, Arha2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization By similarity.

Enzyme regulationi

GTP hydrolysis is stimulated by ARHGAP30 By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 198GTP By similarity
Nucleotide bindingi59 – 635GTP By similarity
Nucleotide bindingi117 – 1204GTP By similarity

GO - Molecular functioni

  1. GDP binding Source: RGD
  2. GTP binding Source: RGD
  3. GTPase activity Source: BHF-UCL
  4. protein binding Source: RGD
  5. protein domain specific binding Source: RGD

GO - Biological processi

  1. GTP catabolic process Source: BHF-UCL
  2. actin cytoskeleton organization Source: RGD
  3. androgen receptor signaling pathway Source: Ensembl
  4. apical junction assembly Source: UniProtKB
  5. cell-matrix adhesion Source: Ensembl
  6. cellular response to progesterone stimulus Source: BHF-UCL
  7. cerebral cortex cell migration Source: Ensembl
  8. cleavage furrow formation Source: UniProtKB
  9. forebrain radial glial cell differentiation Source: Ensembl
  10. negative regulation of I-kappaB kinase/NF-kappaB signaling Source: RGD
  11. negative regulation of cell death Source: RGD
  12. negative regulation of intracellular steroid hormone receptor signaling pathway Source: Ensembl
  13. negative regulation of neuron apoptotic process Source: Ensembl
  14. negative regulation of neuron differentiation Source: RGD
  15. neuron projection morphogenesis Source: RGD
  16. ossification involved in bone maturation Source: Ensembl
  17. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
  18. positive regulation of actin filament polymerization Source: RGD
  19. positive regulation of cell adhesion Source: RGD
  20. positive regulation of cell growth Source: RGD
  21. positive regulation of cell migration Source: RGD
  22. positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: RGD
  23. positive regulation of cytokinesis Source: UniProtKB
  24. positive regulation of neuron apoptotic process Source: RGD
  25. positive regulation of podosome assembly Source: Ensembl
  26. positive regulation of smooth muscle contraction Source: RGD
  27. positive regulation of translation Source: RGD
  28. positive regulation of vasoconstriction Source: RGD
  29. regulation of actin polymerization or depolymerization Source: RGD
  30. regulation of calcium ion transport Source: RGD
  31. regulation of cell migration Source: UniProtKB
  32. regulation of dendrite development Source: RGD
  33. regulation of neural precursor cell proliferation Source: Ensembl
  34. regulation of osteoblast proliferation Source: Ensembl
  35. regulation of transcription from RNA polymerase II promoter Source: Ensembl
  36. response to amino acid Source: RGD
  37. response to drug Source: RGD
  38. response to ethanol Source: RGD
  39. response to glucocorticoid Source: RGD
  40. response to glucose Source: RGD
  41. response to hypoxia Source: RGD
  42. response to mechanical stimulus Source: RGD
  43. skeletal muscle tissue development Source: Ensembl
  44. small GTPase mediated signal transduction Source: InterPro
  45. stress fiber assembly Source: Ensembl
  46. stress-activated protein kinase signaling cascade Source: RGD
  47. trabecula morphogenesis Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_197601. Axonal growth stimulation.
REACT_198389. PCP/CE pathway.
REACT_198828. TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition).

Names & Taxonomyi

Protein namesi
Recommended name:
Transforming protein RhoA
Gene namesi
Name:Rhoa
Synonyms:Arha, Arha2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 8

Organism-specific databases

RGDi619921. Rhoa.

Subcellular locationi

Cell membrane; Lipid-anchor; Cytoplasmic side By similarity. Cytoplasmcytoskeleton By similarity. Cleavage furrow By similarity. Cytoplasmcell cortex By similarity. Midbody By similarity. Cell projectionlamellipodium By similarity
Note: Translocates to the equatorial region before furrow formation in a ECT2-dependent manner. Localizes to the equatorial cell cortex (at the site of the presumptive furrow) in early anaphase in a activated form and in a myosin- and actin-independent manner. Localized to cell-cell contacts in calcium-treated keratinocytes By similarity.

GO - Cellular componenti

  1. apical junction complex Source: UniProtKB
  2. axon Source: RGD
  3. cell cortex Source: UniProtKB
  4. cleavage furrow Source: UniProtKB-SubCell
  5. cytoplasm Source: RGD
  6. cytoskeleton Source: UniProtKB-SubCell
  7. cytosol Source: BHF-UCL
  8. lamellipodium Source: UniProtKB
  9. membrane Source: BHF-UCL
  10. midbody Source: UniProtKB-SubCell
  11. mitochondrion Source: Ensembl
  12. nucleus Source: Ensembl
  13. ruffle membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 190190Transforming protein RhoA
PRO_0000030415Add
BLAST
Propeptidei191 – 1933Removed in mature form By similarity
PRO_0000030416

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei188 – 1881Phosphoserine; by PKG/PRKG1 By similarity
Modified residuei190 – 1901Cysteine methyl ester By similarity
Lipidationi190 – 1901S-geranylgeranyl cysteine By similarity

Post-translational modificationi

Substrate for botulinum ADP-ribosyltransferase By similarity.
Phosphorylation by PRKG1 at Ser-188 inactivates RHOA signaling By similarity.
Ubiquitinated by the BCR(BACURD1) and BCR(BACURD2) E3 ubiquitin ligase complexes, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration By similarity.

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation, Ubl conjugation

Proteomic databases

PaxDbiP61589.
PRIDEiP61589.

2D gel databases

World-2DPAGE0004:P61589.

PTM databases

PhosphoSiteiP61589.

Expressioni

Gene expression databases

ArrayExpressiP61589.
GenevestigatoriP61589.

Interactioni

Subunit structurei

Binds PRKCL1, ROCK1 and ROCK2. Interacts with ARHGEF2, ARHGEF3, NET1 and RTKN. Interacts with PLCE1 and AKAP13. Interacts with ARHGEF28. Interacts with DIAPH1. Interacts (in the constitutively activated, GTP-bound form) with DGKQ. Interacts with GNB2L1/RACK1; enhances RHOA activation. Interacts with PKP4; the interaction is detected at the midbody. Interacts (GTP-bound form preferentially) with PKN2; the interaction stimulates autophosphorylation and phosphorylation of PKN2 By similarity. Interacts with ARHGDIA; this interaction inactivates and stabilizes RHOA. Interacts with ARHGDIB By similarity.

Protein-protein interaction databases

BioGridi250720. 1 interaction.
IntActiP61589. 4 interactions.
MINTiMINT-4542785.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 1310
Helixi18 – 2710
Beta strandi43 – 486
Beta strandi51 – 588
Helixi65 – 695
Helixi70 – 723
Beta strandi78 – 858
Helixi89 – 979
Helixi99 – 1068
Beta strandi112 – 1176
Helixi119 – 1213
Helixi125 – 1339
Helixi141 – 15111
Beta strandi154 – 1585
Turni161 – 1644
Helixi167 – 17913

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3TVDX-ray2.99A/B1-193[»]
ProteinModelPortaliP61589.
SMRiP61589. Positions 2-181.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi34 – 429Effector region Reviewed prediction

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi182 – 1876Arg/Lys-rich (basic)

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00700000104143.
HOGENOMiHOG000233974.
HOVERGENiHBG009351.
InParanoidiP61589.
KOiK04513.
OMAiRNDPHTI.
OrthoDBiEOG73FQPD.
PhylomeDBiP61589.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003578. Small_GTPase_Rho.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00174. RHO. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61589-1 [UniParc]FASTAAdd to Basket

« Hide

MAAIRKKLVI VGDGACGKTC LLIVFSKDQF PEVYVPTVFE NYVADIEVDG    50
KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSIDSPD SLENIPEKWT 100
PEVKHFCPNV PIILVGNKKD LRNDEHTRRE LAKMKQEPVK PEEGRDMANR 150
IGAFGYMECS AKTKDGVREV FEMATRAALQ ARRGKKKSGC LIL 193
Length:193
Mass (Da):21,782
Last modified:May 24, 2004 - v1
Checksum:iC4C8BDC31FF858BC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY026068 mRNA. Translation: AAK11717.1.
AY026069 mRNA. Translation: AAK11718.1.
BC061732 mRNA. Translation: AAH61732.1.
RefSeqiNP_476473.1. NM_057132.3.
XP_006243761.1. XM_006243699.1.
XP_006243762.1. XM_006243700.1.
XP_006243763.1. XM_006243701.1.
UniGeneiRn.107401.

Genome annotation databases

EnsembliENSRNOT00000071664; ENSRNOP00000066672; ENSRNOG00000050519.
GeneIDi117273.
KEGGirno:117273.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY026068 mRNA. Translation: AAK11717.1 .
AY026069 mRNA. Translation: AAK11718.1 .
BC061732 mRNA. Translation: AAH61732.1 .
RefSeqi NP_476473.1. NM_057132.3.
XP_006243761.1. XM_006243699.1.
XP_006243762.1. XM_006243700.1.
XP_006243763.1. XM_006243701.1.
UniGenei Rn.107401.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3TVD X-ray 2.99 A/B 1-193 [» ]
ProteinModelPortali P61589.
SMRi P61589. Positions 2-181.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 250720. 1 interaction.
IntActi P61589. 4 interactions.
MINTi MINT-4542785.

PTM databases

PhosphoSitei P61589.

2D gel databases

World-2DPAGE 0004:P61589.

Proteomic databases

PaxDbi P61589.
PRIDEi P61589.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000071664 ; ENSRNOP00000066672 ; ENSRNOG00000050519 .
GeneIDi 117273.
KEGGi rno:117273.

Organism-specific databases

CTDi 387.
RGDi 619921. Rhoa.

Phylogenomic databases

eggNOGi COG1100.
GeneTreei ENSGT00700000104143.
HOGENOMi HOG000233974.
HOVERGENi HBG009351.
InParanoidi P61589.
KOi K04513.
OMAi RNDPHTI.
OrthoDBi EOG73FQPD.
PhylomeDBi P61589.

Enzyme and pathway databases

Reactomei REACT_197601. Axonal growth stimulation.
REACT_198389. PCP/CE pathway.
REACT_198828. TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition).

Miscellaneous databases

NextBioi 620199.

Gene expression databases

ArrayExpressi P61589.
Genevestigatori P61589.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003578. Small_GTPase_Rho.
[Graphical view ]
Pfami PF00071. Ras. 1 hit.
[Graphical view ]
PRINTSi PR00449. RASTRNSFRMNG.
SMARTi SM00174. RHO. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51420. RHO. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of Wistar-Kyoto (WKY) and spontaneously hypertensive rat (SHR) RhoA cDNA."
    Andresen B.T., Jackson E.K., Romero G.G.
    Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: SHR and Wistar Kyoto.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.

Entry informationi

Entry nameiRHOA_RAT
AccessioniPrimary (citable) accession number: P61589
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 24, 2004
Last modified: September 3, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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