Reviewed, UniProtKB/Swiss-Prot P61585 (RHOA_BOVIN)

Names and origin

Protein names

Recommended name:
    Transforming protein RhoA
Alternative name(s):
    Gb
    p21

Gene names

Name:	RHOA
Synonyms:	ARHA, RHO12

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP By similarity.
Cofactor	Magnesium By similarity.
Subunit structure	Binds PRKCL1, ROCK1 and ROCK2. Interacts with ARHGEF2, ARHGEF3, NET1 and RTKN. Interacts with PLCE1 and AKAP13. Interacts with RGNEF. Interacts with DIAPH1. Interacts (in the constitutively activated, GTP-bound form) with DGKQ By similarity.
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm › cytoskeleton.
Post-translational modification	Substrate for botulinum ADP-ribosyltransferase.
Sequence similarities	Belongs to the small GTPase superfamily. Rho family.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Cytoskeleton Membrane
Disease	Proto-oncogene
Ligand	GTP-binding Magnesium Nucleotide-binding
PTM	ADP-ribosylation Lipoprotein Methylation Phosphoprotein Prenylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	positive regulation of I-kappaB kinase/NF-kappaB cascade Inferred from sequence or structural similarity. Source: UniProtKB small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 190	190	Transforming protein RhoA		PRO_0000030407
	Propeptide	191 – 193	3	Removed in mature form		PRO_0000030408
Regions
	Nucleotide binding	12 – 19	8	GTP
	Nucleotide binding	59 – 63	5	GTP By similarity
	Nucleotide binding	117 – 120	4	GTP
	Motif	34 – 42	9	Effector region Potential
	Compositional bias	182 – 187	6	Arg/Lys-rich (basic)
Amino acid modifications
	Modified residue	41	1	ADP-ribosylasparagine; by botulinum toxin
	Modified residue	156	1	Phosphotyrosine By similarity
	Modified residue	190	1	Cysteine methyl ester
	Lipidation	190	1	S-geranylgeranyl cysteine

Sequences

	Sequence		Length	Mass (Da)
	P61585-1 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: C4DA2DC31FF858BC	FASTA	193	21,768
	10 20 30 40 50 60 MAAIRKKLVI VGDGACGKTC LLIVFSKDQF PEVYVPTVFE NYVADIEVDG KQVELALWDT 70 80 90 100 110 120 AGQEDYDRLR PLSYPDTDVI LMCFSIDSPD SLENIPEKWT PEVKHFCPNV PIILVGNKKD 130 140 150 160 170 180 LRNDEHTRRE LAKMKQEPVK PEEGRDMANR IGAFGYMECS AKTKDGVREV FEMATRAALQ 190 ARRGKKKSGC LVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning of Gb, the substrate for botulinum ADP-ribosyltransferase from bovine adrenal gland and its identification as a rho gene product." Ogorochi T., Nemoto Y., Nakajima M., Nakamura E., Fujiwara M., Narumiya S. Biochem. Biophys. Res. Commun. 163:1175-1181(1989) [PubMed: 2506852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.
[3]	"Substrate for botulinum ADP-ribosyltransferase, Gb, has an amino acid sequence homologous to a putative rho gene product." Narumiya S., Sekine A., Fujiwara M. J. Biol. Chem. 263:17255-17257(1988) [PubMed: 3141419] [Abstract] Cited for: PROTEIN SEQUENCE OF 19-25; 42-50; 52-57; 59-97; 99-104; 134-162 AND 169-176. Tissue: Adrenal gland.
[4]	"Guanine nucleotide-dependent ADP-ribosylation of soluble rho catalyzed by Clostridium botulinum C3 ADP-ribosyltransferase. Isolation and characterization of a newly recognized form of rhoA." Williamson K.C., Smith L.A., Moss J., Vaughan M. J. Biol. Chem. 265:20807-20812(1990) [PubMed: 2174426] [Abstract] Cited for: PROTEIN SEQUENCE OF 28-70 AND 99-194, ADP-RIBOSYLATION AT ASN-41.
[5]	"Asparagine residue in the rho gene product is the modification site for botulinum ADP-ribosyltransferase." Sekine A., Fujiwara M., Narumiya S. J. Biol. Chem. 264:8602-8605(1989) [PubMed: 2498316] [Abstract] Cited for: PROTEIN SEQUENCE OF 37-47, ADP-RIBOSYLATION AT ASN-41.
[6]	"The posttranslationally modified C-terminal structure of bovine aortic smooth muscle rhoA p21." Katayama M., Kawata M., Yoshida Y., Horiuchi H., Yamamoto T., Matsuura Y., Takai Y. J. Biol. Chem. 266:12639-12645(1991) [PubMed: 1905729] [Abstract] Cited for: ISOPRENYLATION AT CYS-190. Tissue: Aortic smooth muscle.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M27278 mRNA. Translation: AAA30409.1. BC102880 mRNA. Translation: AAI02881.1.
IPI	IPI00688998.
PIR	TVBO12. A33518.
RefSeq	NP_788818.1.
UniGene	Bt.49678
3D structure databases
SMR	P61585. Positions 1-181.
ModBase	Search...
Protein-protein interaction databases
STRING	P61585.
Proteomic databases
PRIDE	P61585.
Genome annotation databases
Ensembl	ENSBTAT00000005600; ENSBTAP00000005600; ENSBTAG00000004279; Bos taurus. [Genome view]
GeneID	338049.
KEGG	bta:338049.
Organism-specific databases
CTD	338049.
Phylogenomic databases
HOVERGEN	P61585.
OMA	KWIAEVL
OrthoDB	EOG969TCZ
Family and domain databases
InterPro	IPR003578. GTPase_Rho. IPR013753. Ras. IPR001806. Ras_GTPase. IPR005225. Small_GTP_bd. [Graphical view]
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00174. RHO. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51420. RHO. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RHOA_BOVIN

Accession

Primary (citable) accession number: P61585
Secondary accession number(s): P06749, Q3ZC72, Q9UEJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	November 24, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)