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Protein

Argininosuccinate synthase

Gene

argG

Organism
Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei87CitrullineUniRule annotation1
Binding sitei117ATP; via amide nitrogenUniRule annotation1
Binding sitei119AspartateUniRule annotation1
Binding sitei123AspartateUniRule annotation1
Binding sitei123CitrullineUniRule annotation1
Binding sitei124AspartateUniRule annotation1
Binding sitei127CitrullineUniRule annotation1
Binding sitei175CitrullineUniRule annotation1
Binding sitei259CitrullineUniRule annotation1
Binding sitei271CitrullineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 16ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Arginine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:DIP1173
OrganismiCorynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Taxonomic identifieri257309 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000002198 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001485881 – 399Argininosuccinate synthaseAdd BLAST399

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP61521.
SMRiP61521.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000230093.
KOiK01940.
OMAiGVGRIDM.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
PANTHERiPTHR11587. PTHR11587. 1 hit.
PfamiView protein in Pfam
PF00764. Arginosuc_synth. 1 hit.
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiView protein in PROSITE
PS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P61521-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNRVVLAYS GGLDTSVAIP YLAKMTGGEV VAVSLDLGQG GEDMESVRQR
60 70 80 90 100
ALDCGAVESI VIDAKDEFAN DYCLPTIKAN GMYMKQYPLV SAISRPLIVK
110 120 130 140 150
HLVEAAKKHG GTHVSHGCTG KGNDQVRFEV GFRNLAPELQ IIAPARDYAW
160 170 180 190 200
TRDKAIAFAE EINLPIEQSK KSPFSIDQNV WGRAVETGFL EDLWNPPTKD
210 220 230 240 250
LYSYTEDPAL GNAPDEIIIS FKAGVPVAID GRPVTVLEAI EEMNRRAGAQ
260 270 280 290 300
GIGRLDMVED RLVGIKSREV YEAPGAIALI TAHQAMEDVT IERELARYKR
310 320 330 340 350
GIDARWSEEV YDGLWYAPLK RSLDAFIENT QEHVTGDIRM VMHAGKCTVN
360 370 380 390
GRRSEHSLYD FDLATYDTGD TFDQTLAKGF VELHGLSSKI SNKRDREAQ
Length:399
Mass (Da):44,082
Last modified:May 24, 2004 - v1
Checksum:iB83FD37285891D64
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248357 Genomic DNA. Translation: CAE49693.1.
RefSeqiWP_003851325.1. NC_002935.2.

Genome annotation databases

EnsemblBacteriaiCAE49693; CAE49693; DIP1173.
KEGGicdi:DIP1173.

Similar proteinsi

Entry informationi

Entry nameiASSY_CORDI
AccessioniPrimary (citable) accession number: P61521
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 24, 2004
Last modified: August 30, 2017
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families