Reviewed, UniProtKB/Swiss-Prot P61518 (CAN_SHIFL)

Names and origin

Protein names

Recommended name:
    Carbonic anhydrase 2
    EC=4.2.1.1
Alternative name(s):
    Carbonate dehydratase 2

Gene names

Name:	can
Ordered Locus Names:	SF0123, S0125

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	H2CO3 = CO2 + H2O.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the beta-class carbonic anhydrase family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbon utilization Inferred from electronic annotation. Source: InterPro
Molecular function	carbonate dehydratase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 220	220	Carbonic anhydrase 2		PRO_0000077466
Sites
	Metal binding	42	1	Zinc By similarity
	Metal binding	44	1	Zinc By similarity
	Metal binding	98	1	Zinc By similarity
	Metal binding	101	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	P61518-1 [UniParc]. Last modified May 24, 2004. Version 1. Checksum: 48A9086BE9428452	FASTA	220	25,097
	10 20 30 40 50 60 MKDIDTLISN NALWSKMLVE EDPGFFEKLA QAQKPRFLWI GCSDSRVPAE RLTGLEPGEL 70 80 90 100 110 120 FVHRNVANLV IHTDLNCLSV VQYAVDVLEV EHIIICGHYG CGGVQAAVEN PELGLINNWL 130 140 150 160 170 180 LHIRDIWFKH SSLLGEMPQE RRLDTLCELN VMEQVYNLGH STIMQSAWKR GQKVTIHGWA 190 200 210 220 YGIHDGLLRD LDVTATNRET LEQRYRHGIS NLKLKHANHK

References

[1]	"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed: 12384590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.
[2]	"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed: 12704152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005674 Genomic DNA. Translation: AAN41786.1. AE014073 Genomic DNA. Translation: AAP15667.1.
RefSeq	NP_706079.1. NP_835862.1.
3D structure databases
SMR	P61518. Positions 2-215.
ModBase	Search...
Genome annotation databases
GeneID	1024493. 1076556.
GenomeReviews	Gene locus SF0123 in contig AE005674_GR. Gene locus S0125 in contig AE014073_GR.
KEGG	sfl:SF0123. sfx:S0125.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P61518.
OMA	VQEAWAR.
Enzyme and pathway databases
BioCyc	SFLE198214:AAN41786.1-MON.
BRENDA	4.2.1.1. 189495.
Family and domain databases
InterPro	IPR001765. Carbonic_anhydrase. IPR015892. Carbonic_anhydrase_CS. [Graphical view]
Gene3D	G3DSA:3.40.1050.10. CO_anhd_prok_pln. 1 hit.
PANTHER	PTHR11002. Carbonic_anhydrase. 1 hit.
Pfam	PF00484. Pro_CA. 1 hit. [Graphical view]
PROSITE	PS00704. PROK_CO2_ANHYDRASE_1. 1 hit. PS00705. PROK_CO2_ANHYDRASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAN_SHIFL

Accession

Primary (citable) accession number: P61518
Secondary accession number(s): P36857, P75656, Q8KJQ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	May 24, 2004
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)