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P61517

- CAN_ECOLI

UniProt

P61517 - CAN_ECOLI

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Protein

Carbonic anhydrase 2

Gene

can

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

H2CO3 = CO2 + H2O.

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi42 – 421Zinc
Metal bindingi44 – 441Zinc
Metal bindingi98 – 981Zinc
Metal bindingi101 – 1011Zinc

GO - Molecular functioni

  1. carbonate dehydratase activity Source: EcoCyc
  2. zinc ion binding Source: EcoCyc

GO - Biological processi

  1. carbon utilization Source: InterPro
  2. metabolic process Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:EG12319-MONOMER.
ECOL316407:JW0122-MONOMER.
BRENDAi4.2.1.1. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbonic anhydrase 2 (EC:4.2.1.1)
Alternative name(s):
Carbonate dehydratase 2
Gene namesi
Name:can
Synonyms:cynT2, yadF
Ordered Locus Names:b0126, JW0122
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG12319. can.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 220220Carbonic anhydrase 2PRO_0000077465Add
BLAST

Proteomic databases

PaxDbiP61517.
PRIDEiP61517.

Expressioni

Gene expression databases

GenevestigatoriP61517.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

DIPiDIP-36168N.
IntActiP61517. 10 interactions.
STRINGi511145.b0126.

Structurei

Secondary structure

1
220
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1916Combined sources
Turni23 – 264Combined sources
Helixi27 – 304Combined sources
Beta strandi36 – 427Combined sources
Helixi49 – 535Combined sources
Beta strandi59 – 657Combined sources
Helixi75 – 8612Combined sources
Beta strandi91 – 988Combined sources
Helixi102 – 1098Combined sources
Helixi116 – 12914Combined sources
Helixi131 – 1344Combined sources
Helixi139 – 1413Combined sources
Helixi142 – 16019Combined sources
Helixi162 – 1698Combined sources
Beta strandi175 – 1817Combined sources
Turni183 – 1853Combined sources
Beta strandi188 – 1903Combined sources
Beta strandi195 – 1973Combined sources
Helixi198 – 21417Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1I6OX-ray2.20A/B1-220[»]
1I6PX-ray2.00A1-220[»]
1T75X-ray2.50A/B/D/E1-220[»]
2ESFX-ray2.25A/B1-220[»]
ProteinModelPortaliP61517.
SMRiP61517. Positions 2-215.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61517.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0288.
HOGENOMiHOG000125184.
InParanoidiP61517.
KOiK01673.
OMAiCEINVIE.
OrthoDBiEOG6FFSB8.
PhylomeDBiP61517.

Family and domain databases

Gene3Di3.40.1050.10. 1 hit.
InterProiIPR001765. Carbonic_anhydrase.
IPR015892. Carbonic_anhydrase_CS.
[Graphical view]
PANTHERiPTHR11002. PTHR11002. 1 hit.
PfamiPF00484. Pro_CA. 1 hit.
[Graphical view]
SMARTiSM00947. Pro_CA. 1 hit.
[Graphical view]
SUPFAMiSSF53056. SSF53056. 1 hit.
PROSITEiPS00704. PROK_CO2_ANHYDRASE_1. 1 hit.
PS00705. PROK_CO2_ANHYDRASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P61517-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKDIDTLISN NALWSKMLVE EDPGFFEKLA QAQKPRFLWI GCSDSRVPAE
60 70 80 90 100
RLTGLEPGEL FVHRNVANLV IHTDLNCLSV VQYAVDVLEV EHIIICGHYG
110 120 130 140 150
CGGVQAAVEN PELGLINNWL LHIRDIWFKH SSLLGEMPQE RRLDTLCELN
160 170 180 190 200
VMEQVYNLGH STIMQSAWKR GQKVTIHGWA YGIHDGLLRD LDVTATNRET
210 220
LEQRYRHGIS NLKLKHANHK
Length:220
Mass (Da):25,097
Last modified:May 24, 2004 - v1
Checksum:i48A9086BE9428452
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73237.1.
AP009048 Genomic DNA. Translation: BAB96701.2.
PIRiF64735.
RefSeqiNP_414668.1. NC_000913.3.
YP_488429.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73237; AAC73237; b0126.
BAB96701; BAB96701; BAB96701.
GeneIDi12930739.
944832.
KEGGiecj:Y75_p0123.
eco:b0126.
PATRICi32115355. VBIEscCol129921_0129.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73237.1 .
AP009048 Genomic DNA. Translation: BAB96701.2 .
PIRi F64735.
RefSeqi NP_414668.1. NC_000913.3.
YP_488429.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1I6O X-ray 2.20 A/B 1-220 [» ]
1I6P X-ray 2.00 A 1-220 [» ]
1T75 X-ray 2.50 A/B/D/E 1-220 [» ]
2ESF X-ray 2.25 A/B 1-220 [» ]
ProteinModelPortali P61517.
SMRi P61517. Positions 2-215.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-36168N.
IntActi P61517. 10 interactions.
STRINGi 511145.b0126.

Proteomic databases

PaxDbi P61517.
PRIDEi P61517.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73237 ; AAC73237 ; b0126 .
BAB96701 ; BAB96701 ; BAB96701 .
GeneIDi 12930739.
944832.
KEGGi ecj:Y75_p0123.
eco:b0126.
PATRICi 32115355. VBIEscCol129921_0129.

Organism-specific databases

EchoBASEi EB2224.
EcoGenei EG12319. can.

Phylogenomic databases

eggNOGi COG0288.
HOGENOMi HOG000125184.
InParanoidi P61517.
KOi K01673.
OMAi CEINVIE.
OrthoDBi EOG6FFSB8.
PhylomeDBi P61517.

Enzyme and pathway databases

BioCyci EcoCyc:EG12319-MONOMER.
ECOL316407:JW0122-MONOMER.
BRENDAi 4.2.1.1. 2026.

Miscellaneous databases

EvolutionaryTracei P61517.
PROi P61517.

Gene expression databases

Genevestigatori P61517.

Family and domain databases

Gene3Di 3.40.1050.10. 1 hit.
InterProi IPR001765. Carbonic_anhydrase.
IPR015892. Carbonic_anhydrase_CS.
[Graphical view ]
PANTHERi PTHR11002. PTHR11002. 1 hit.
Pfami PF00484. Pro_CA. 1 hit.
[Graphical view ]
SMARTi SM00947. Pro_CA. 1 hit.
[Graphical view ]
SUPFAMi SSF53056. SSF53056. 1 hit.
PROSITEi PS00704. PROK_CO2_ANHYDRASE_1. 1 hit.
PS00705. PROK_CO2_ANHYDRASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region."
    Fujita N., Mori H., Yura T., Ishihama A.
    Nucleic Acids Res. 22:1637-1639(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography."
    Fountoulakis M., Takacs M.-F., Berndt P., Langen H., Takacs B.
    Electrophoresis 20:2181-2195(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: B / BL21.
  5. "Crystal structure of E. coli beta-carbonic anhydrase, an enzyme with an unusual pH-dependent activity."
    Cronk J.D., Endrizzi J.A., Cronk M.R., O'neill J.W., Zhang K.Y.J.
    Protein Sci. 10:911-922(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).

Entry informationi

Entry nameiCAN_ECOLI
AccessioniPrimary (citable) accession number: P61517
Secondary accession number(s): P36857, P75656, Q8KJQ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 24, 2004
Last modified: November 26, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3