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P61517

- CAN_ECOLI

UniProt

P61517 - CAN_ECOLI

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Protein
Carbonic anhydrase 2
Gene
can, cynT2, yadF, b0126, JW0122
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

H2CO3 = CO2 + H2O.

Cofactori

Binds 1 zinc ion per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi42 – 421Zinc
Metal bindingi44 – 441Zinc
Metal bindingi98 – 981Zinc
Metal bindingi101 – 1011Zinc

GO - Molecular functioni

  1. carbonate dehydratase activity Source: EcoCyc
  2. zinc ion binding Source: EcoCyc

GO - Biological processi

  1. carbon utilization Source: InterPro
  2. metabolic process Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:EG12319-MONOMER.
ECOL316407:JW0122-MONOMER.
BRENDAi4.2.1.1. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbonic anhydrase 2 (EC:4.2.1.1)
Alternative name(s):
Carbonate dehydratase 2
Gene namesi
Name:can
Synonyms:cynT2, yadF
Ordered Locus Names:b0126, JW0122
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG12319. can.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 220220Carbonic anhydrase 2
PRO_0000077465Add
BLAST

Proteomic databases

PaxDbiP61517.
PRIDEiP61517.

Expressioni

Gene expression databases

GenevestigatoriP61517.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

DIPiDIP-36168N.
IntActiP61517. 10 interactions.
STRINGi511145.b0126.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1916
Turni23 – 264
Helixi27 – 304
Beta strandi36 – 427
Helixi49 – 535
Beta strandi59 – 657
Helixi75 – 8612
Beta strandi91 – 988
Helixi102 – 1098
Helixi116 – 12914
Helixi131 – 1344
Helixi139 – 1413
Helixi142 – 16019
Helixi162 – 1698
Beta strandi175 – 1817
Turni183 – 1853
Beta strandi188 – 1903
Beta strandi195 – 1973
Helixi198 – 21417

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1I6OX-ray2.20A/B1-220[»]
1I6PX-ray2.00A1-220[»]
1T75X-ray2.50A/B/D/E1-220[»]
2ESFX-ray2.25A/B1-220[»]
ProteinModelPortaliP61517.
SMRiP61517. Positions 2-215.

Miscellaneous databases

EvolutionaryTraceiP61517.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0288.
HOGENOMiHOG000125184.
KOiK01673.
OMAiCEINVIE.
OrthoDBiEOG6FFSB8.
PhylomeDBiP61517.

Family and domain databases

Gene3Di3.40.1050.10. 1 hit.
InterProiIPR001765. Carbonic_anhydrase.
IPR015892. Carbonic_anhydrase_CS.
[Graphical view]
PANTHERiPTHR11002. PTHR11002. 1 hit.
PfamiPF00484. Pro_CA. 1 hit.
[Graphical view]
SMARTiSM00947. Pro_CA. 1 hit.
[Graphical view]
SUPFAMiSSF53056. SSF53056. 1 hit.
PROSITEiPS00704. PROK_CO2_ANHYDRASE_1. 1 hit.
PS00705. PROK_CO2_ANHYDRASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P61517-1 [UniParc]FASTAAdd to Basket

« Hide

MKDIDTLISN NALWSKMLVE EDPGFFEKLA QAQKPRFLWI GCSDSRVPAE    50
RLTGLEPGEL FVHRNVANLV IHTDLNCLSV VQYAVDVLEV EHIIICGHYG 100
CGGVQAAVEN PELGLINNWL LHIRDIWFKH SSLLGEMPQE RRLDTLCELN 150
VMEQVYNLGH STIMQSAWKR GQKVTIHGWA YGIHDGLLRD LDVTATNRET 200
LEQRYRHGIS NLKLKHANHK 220
Length:220
Mass (Da):25,097
Last modified:May 24, 2004 - v1
Checksum:i48A9086BE9428452
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC73237.1.
AP009048 Genomic DNA. Translation: BAB96701.2.
PIRiF64735.
RefSeqiNP_414668.1. NC_000913.3.
YP_488429.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73237; AAC73237; b0126.
BAB96701; BAB96701; BAB96701.
GeneIDi12930739.
944832.
KEGGiecj:Y75_p0123.
eco:b0126.
PATRICi32115355. VBIEscCol129921_0129.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC73237.1 .
AP009048 Genomic DNA. Translation: BAB96701.2 .
PIRi F64735.
RefSeqi NP_414668.1. NC_000913.3.
YP_488429.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1I6O X-ray 2.20 A/B 1-220 [» ]
1I6P X-ray 2.00 A 1-220 [» ]
1T75 X-ray 2.50 A/B/D/E 1-220 [» ]
2ESF X-ray 2.25 A/B 1-220 [» ]
ProteinModelPortali P61517.
SMRi P61517. Positions 2-215.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-36168N.
IntActi P61517. 10 interactions.
STRINGi 511145.b0126.

Proteomic databases

PaxDbi P61517.
PRIDEi P61517.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73237 ; AAC73237 ; b0126 .
BAB96701 ; BAB96701 ; BAB96701 .
GeneIDi 12930739.
944832.
KEGGi ecj:Y75_p0123.
eco:b0126.
PATRICi 32115355. VBIEscCol129921_0129.

Organism-specific databases

EchoBASEi EB2224.
EcoGenei EG12319. can.

Phylogenomic databases

eggNOGi COG0288.
HOGENOMi HOG000125184.
KOi K01673.
OMAi CEINVIE.
OrthoDBi EOG6FFSB8.
PhylomeDBi P61517.

Enzyme and pathway databases

BioCyci EcoCyc:EG12319-MONOMER.
ECOL316407:JW0122-MONOMER.
BRENDAi 4.2.1.1. 2026.

Miscellaneous databases

EvolutionaryTracei P61517.
PROi P61517.

Gene expression databases

Genevestigatori P61517.

Family and domain databases

Gene3Di 3.40.1050.10. 1 hit.
InterProi IPR001765. Carbonic_anhydrase.
IPR015892. Carbonic_anhydrase_CS.
[Graphical view ]
PANTHERi PTHR11002. PTHR11002. 1 hit.
Pfami PF00484. Pro_CA. 1 hit.
[Graphical view ]
SMARTi SM00947. Pro_CA. 1 hit.
[Graphical view ]
SUPFAMi SSF53056. SSF53056. 1 hit.
PROSITEi PS00704. PROK_CO2_ANHYDRASE_1. 1 hit.
PS00705. PROK_CO2_ANHYDRASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region."
    Fujita N., Mori H., Yura T., Ishihama A.
    Nucleic Acids Res. 22:1637-1639(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography."
    Fountoulakis M., Takacs M.-F., Berndt P., Langen H., Takacs B.
    Electrophoresis 20:2181-2195(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: B / BL21.
  5. "Crystal structure of E. coli beta-carbonic anhydrase, an enzyme with an unusual pH-dependent activity."
    Cronk J.D., Endrizzi J.A., Cronk M.R., O'neill J.W., Zhang K.Y.J.
    Protein Sci. 10:911-922(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).

Entry informationi

Entry nameiCAN_ECOLI
AccessioniPrimary (citable) accession number: P61517
Secondary accession number(s): P36857, P75656, Q8KJQ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 24, 2004
Last modified: June 11, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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