P61498 (PYRE_THET2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Orotate phosphoribosyltransferase
Short name=OPRT
Short name=OPRTase
EC=2.4.2.10

Gene names

Name:	pyrE
Ordered Locus Names:	TT_C1380

Organism

Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) [Complete proteome] [HAMAP]

Taxonomic identifier

262724 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	183 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. Ref.1
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. Ref.1
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity. HAMAP MF_01208
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 183	183	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_0000110760
Regions
	Region	112 – 120	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	21	1	5-phosphoribose 1-diphosphate By similarity
	Binding site	88	1	5-phosphoribose 1-diphosphate By similarity
	Binding site	116	1	Orotate By similarity
	Binding site	144	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P61498 [UniParc]. Last modified May 24, 2004. Version 1. Checksum: DDD3BB0235AEB9ED	FASTA	183	19,442
	10 20 30 40 50 60 MDVLELYRRT GALLEGHFLL RSGMHSPFFL QSAALLQHPL YAEAVGEALG KLFEDEKVDF 70 80 90 100 110 120 VIAPAIGGVV LSFVVAKALG ARALFAEKDG RGGMLIRKGL TVNPGDRFLA VEDVVTTGES 130 140 150 160 170 180 VRKAIRAAEA RGGVLVGVGA IVDRSGGRAA FGVPFRALLA LEVPQYPEEA CPLCREGVPL EEV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Pyrimidine biosynthesis genes (pyrE and pyrF) of an extreme thermophile, Thermus thermophilus." Yamagishi A., Tanimoto T., Suzuki T., Oshima T. Appl. Environ. Microbiol. 62:2191-2194(1996) [PubMed: 8787418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY.
[2]	"The genome sequence of the extreme thermophile Thermus thermophilus." Henne A., Brueggemann H., Raasch C., Wiezer A., Hartsch T., Liesegang H., Johann A., Lienard T., Gohl O., Martinez-Arias R., Jacobi C., Starkuviene V., Schlenczeck S., Dencker S., Huber R., Klenk H.-P., Kramer W., Merkl R., Gottschalk G., Fritz H.-J. Nat. Biotechnol. 22:547-553(2004) [PubMed: 15064768] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB27 / ATCC BAA-163 / DSM 7039.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D83330 Genomic DNA. Translation: BAA11886.1. AE017221 Genomic DNA. Translation: AAS81722.1.
RefSeq	YP_005349.1. NC_005835.1.
3D structure databases
ProteinModelPortal	P61498.
ModBase	Search...
Protein-protein interaction databases
STRING	P61498.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2776393.
GenomeReviews	Gene locus TT_C1380 in contig AE017221_GR.
PATRIC	23953173. VBITheThe54392_1373.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0461.
HOGENOM	HBG404341.
OMA	FYLQSAK.
PhylomeDB	P61498.
ProtClustDB	PRK00455.
Enzyme and pathway databases
BioCyc	TTHE262724:TT_C1380-MONOMER.
Family and domain databases
HAMAP	MF_01208. PyrE. [Tree]
InterPro	IPR023031. Orotate_PribosylTferase. IPR006273. Orotate_PRibTrfase_thermus-typ. IPR000836. PRibTrfase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01367. PyrE_Therm. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_THET2

Accession

Primary (citable) accession number: P61498
Secondary accession number(s): O08047, O08276, Q60016

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	May 24, 2004
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program