Reviewed, UniProtKB/Swiss-Prot P61489 (AK_THETH)

Names and origin

Protein names

Recommended name:
    Aspartokinase
    EC=2.7.2.4
Alternative name(s):
    Aspartate kinase
      Short name=ASK

Gene names

Name:	ask
Synonyms:	askAB

Organism

Thermus thermophilus

Taxonomic identifier

274 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	405 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate = ADP + 4-phospho-L-aspartate.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; tetrahydrodipicolinate from L-aspartate: step 1/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.
Subunit structure	Tetramer consisting of 2 isoforms Alpha (catalytic) and 2 isoforms Beta (function not known) By similarity.
Sequence similarities	Belongs to the aspartokinase family. Contains 2 ACT domains.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Coding sequence diversity	Alternative initiation
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW amino acid binding Inferred from electronic annotation. Source: InterPro aspartate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform Alpha (identifier: P61489-1)

Also known as: Aspartokinase subunit alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Beta (identifier: P61489-2)

Also known as: Aspartokinase subunit beta;

The sequence of this isoform differs from the canonical sequence as follows:
     1-244: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 405	405	Aspartokinase		PRO_0000002389
Regions
	Domain	262 – 335	74	ACT 1
	Domain	343 – 404	62	ACT 2
Natural variations
	Alternative sequence	1 – 244	244	Missing in isoform Beta.		VSP_018665

Sequences

	Sequence		Length	Mass (Da)
	Isoform Alpha (Aspartokinase subunit alpha) [UniParc]. Last modified May 24, 2004. Version 1. Checksum: 47A3A29E2B82D0EA	FASTA	405	43,319
	10 20 30 40 50 60 MALVVQKYGG TSVGDLERIH KVAQRIAHYR EKGHRLAVVV SAMGHTTDEL IALAKRVNPR 70 80 90 100 110 120 PPFRELDLLT TTGEQVSVAL LSMQLWAMGI PAKGFVQHQI GITTDGRYGD ARILEVNPAR 130 140 150 160 170 180 IREALDQGFV AVIAGFMGTT PEGEITTLGR GGSDTTAVAI AAALGAKECE IYTDTEGVYT 190 200 210 220 230 240 TDPHLIPEAR KLSVIGYDQM LEMAALGARV LHPRAVYYAK RYGVVLHVRS SFSYNPGTLV 250 260 270 280 290 300 KEVAMEMDKA VTGVALDLDH AQIGLIGIPD QPGIAAKVFQ ALAERGIAVD MIIQGVPGHD 310 320 330 340 350 360 PSRQQMAFTV KKDFAQEALE ALEPVLAEIG GEAILRPDIA KVSIVGVGLA STPEVPAKMF 370 380 390 400 QAVASTGANI EMIATSEVRI SVIIPAEYAE AALRAVHQAF ELDKA
	Isoform Beta (Aspartokinase subunit beta). Checksum: 94BDE8A671CC54EA	FASTA	161	16,895
	10 20 30 40 50 60 MEMDKAVTGV ALDLDHAQIG LIGIPDQPGI AAKVFQALAE RGIAVDMIIQ GVPGHDPSRQ 70 80 90 100 110 120 QMAFTVKKDF AQEALEALEP VLAEIGGEAI LRPDIAKVSI VGVGLASTPE VPAKMFQAVA 130 140 150 160 STGANIEMIA TSEVRISVII PAEYAEAALR AVHQAFELDK A

References

[1]	"An operon encoding aspartokinase and purine phosphoribosyltransferase in Thermus flavus." Nishiyama M., Kukimoto M., Beppu T., Horiouchi S. Microbiology 141:1211-1219(1995) [PubMed: 7773416] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 33923 / DSM 674 / AT-62.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

D37928 Genomic DNA. Translation: BAA07146.1.
D37928 Genomic DNA. Translation: BAA07147.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DT9	X-ray	2.15	A/B	245-405	[»]
2ZHO	X-ray	2.98	A/B/C/D/E/F	245-405	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

2.7.2.4. 245.

Family and domain databases

InterPro

IPR002912. ACT_bd.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR005260. Asp_kin_monofn.
IPR001341. Asp_kin_reg.
IPR018042. Aspartate_kinase_CS.
[Graphical view]

Gene3D

G3DSA:3.40.1160.10. Aa_kinase. 1 hit.

Pfam

PF00696. AA_kinase. 1 hit.
PF01842. ACT. 2 hits.
[Graphical view]

TIGRFAMs

TIGR00656. asp_kin_monofn. 1 hit.
TIGR00657. asp_kinases. 1 hit.

PROSITE

PS00324. ASPARTOKINASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

AK_THETH

Accession

Primary (citable) accession number: P61489
Secondary accession number(s): P77991, P97151

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	May 24, 2004
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)