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P61488 (AK_THET2) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Aspartokinase
EC=2.7.2.4
Alternative name(s):
Aspartate kinase
Short name=ASK
Gene names
Name:ask
Synonyms:askAB
Ordered Locus Names:TT_C0166
OrganismThermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) [Complete proteome] [HAMAP]
Taxonomic identifier262724 [NCBI]
Taxonomic lineageBacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus ›

Protein attributes

Sequence length405 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of the beta-carboxyl group of aspartic acid with ATP to yield 4-phospho-L-aspartate, which is involved in the branched biosynthetic pathway leading to the biosynthesis of amino acids lysine, threonine, isoleucine and methionine. Ref.1

Catalytic activity

ATP + L-aspartate = ADP + 4-phospho-L-aspartate.

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4.

Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3.

Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.

Subunit structure

Tetramer consisting of 2 isoforms Alpha (catalytic and regulation) and of a homodimer of 2 isoforms Beta (regulation) By similarity.

Disruption phenotype

Cells lacking this gene show auxotrophy for methionine and threonine. Ref.1

Sequence similarities

Belongs to the aspartokinase family.

Contains 2 ACT domains.

Sequence caution

The sequence AAS80514.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform Alpha (identifier: P61488-1)

Also known as: Aspartokinase subunit alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Beta (identifier: P61488-2)

Also known as: Aspartokinase subunit beta;

The sequence of this isoform differs from the canonical sequence as follows:
     1-244: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 405405Aspartokinase
PRO_0000002387

Regions

Domain262 – 33574ACT 1
Domain343 – 40462ACT 2
Region7 – 104ATP binding By similarity
Region25 – 306Substrate binding By similarity
Region47 – 493Substrate binding By similarity
Region125 – 1262Substrate binding By similarity
Region150 – 1534Substrate binding By similarity
Region173 – 1742ATP binding By similarity
Region179 – 1846ATP binding By similarity
Region288 – 2903Substrate binding By similarity
Region355 – 3562Substrate binding By similarity
Region369 – 3702Substrate binding By similarity
Region376 – 3772Substrate binding By similarity

Sites

Binding site411ATP By similarity
Binding site741Substrate By similarity
Binding site1531Substrate By similarity
Binding site2091ATP By similarity
Binding site2701Substrate By similarity
Binding site2941Substrate By similarity
Site71Contribution to the catalysis By similarity
Site741Contribution to the catalysis By similarity

Natural variations

Alternative sequence1 – 244244Missing in isoform Beta.
VSP_018664

Sequences

Sequence LengthMass (Da)Tools
Isoform Alpha (Aspartokinase subunit alpha) [UniParc].

Last modified May 24, 2004. Version 1.
Checksum: 06F7B79E908960EA

FASTA40543,333
        10         20         30         40         50         60 
MALVVQKYGG TSVGDLERIH KVAQRIAHYR EKGHRLAVVV SAMGHTTDEL IALAKRVNPR 

        70         80         90        100        110        120 
PPFRELDLLT TTGEQVSVAL LSMQLWAMGI PAKGFVQHQI GITTDGRYGD ARILEVNPAR 

       130        140        150        160        170        180 
IREALEQGFV AVIAGFMGTT PEGEITTLGR GGSDTTAVAI AAALGAKECE IYTDTEGVYT 

       190        200        210        220        230        240 
TDPHLIPEAR KLSVIGYDQM LEMAALGARV LHPRAVYYAK RYGVVLHVRS SFSYNPGTLV 

       250        260        270        280        290        300 
KEVAMEMDKA VTGVALDLDH AQIGLIGIPD QPGIAAKVFQ ALAERGIAVD MIIQGVPGHD 

       310        320        330        340        350        360 
PSRQQMAFTV KKDFAQEALE ALEPVLAEIG GEAILRPDIA KVSIVGVGLA STPEVPAKMF 

       370        380        390        400 
QAVASTGANI EMIATSEVRI SVIIPAEYAE AALRAVHQAF ELDKA

« Hide

Isoform Beta (Aspartokinase subunit beta) [UniParc].

Checksum: 94BDE8A671CC54EA
Show »

FASTA16116,895

References

« Hide 'large scale' references
[1]"Aspartate kinase-independent lysine synthesis in an extremely thermophilic bacterium, Thermus thermophilus: lysine is synthesized via alpha-aminoadipic acid not via diaminopimelic acid."
Kobashi N., Nishiyama M., Tanokura M.
J. Bacteriol. 181:1713-1718(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE.
[2]"The genome sequence of the extreme thermophile Thermus thermophilus."
Henne A., Brueggemann H., Raasch C., Wiezer A., Hartsch T., Liesegang H., Johann A., Lienard T., Gohl O., Martinez-Arias R., Jacobi C., Starkuviene V., Schlenczeck S., Dencker S., Huber R., Klenk H.-P., Kramer W., Merkl R., Gottschalk G., Fritz H.-J.
Nat. Biotechnol. 22:547-553(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB27 / ATCC BAA-163 / DSM 7039.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB013131 Genomic DNA. Translation: BAA25848.1.
AB013131 Genomic DNA. Translation: BAA25849.1.
AE017221 Genomic DNA. Translation: AAS80514.1. Different initiation.
RefSeqYP_004141.1. NC_005835.1.

3D structure databases

ProteinModelPortalP61488.
SMRP61488. Positions 249-401.
ModBaseSearch...

Protein-protein interaction databases

STRING262724.TTC0166.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAS80514; AAS80514; TT_C0166.
GeneID2775578.
KEGGtth:TTC0166.
PATRIC23950725. VBITheThe54392_0167.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0527.
KOK00928.
OMASVDMIIQ.
ProtClustDBPRK06635.

Enzyme and pathway databases

BioCycTTHE262724:GCAT-170-MONOMER.
UniPathwayUPA00034; UER00015.
UPA00050; UER00461.
UPA00051; UER00462.

Family and domain databases

Gene3D3.40.1160.10. 1 hit.
InterProIPR002912. ACT_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR005260. Asp_kin_monofn.
IPR001341. Asp_kinase_dom.
IPR018042. Aspartate_kinase_CS.
[Graphical view]
PfamPF00696. AA_kinase. 1 hit.
PF01842. ACT. 1 hit.
[Graphical view]
PIRSFPIRSF000726. Asp_kin. 1 hit.
SUPFAMSSF53633. Aa_kinase. 1 hit.
TIGRFAMsTIGR00656. asp_kin_monofn. 1 hit.
TIGR00657. asp_kinases. 1 hit.
PROSITEPS00324. ASPARTOKINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAK_THET2
AccessionPrimary (citable) accession number: P61488
Secondary accession number(s): P77991, P97151
Entry history
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 24, 2004
Last modified: May 29, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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