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Reviewed, UniProtKB/Swiss-Prot P61488 (AK_THET2)

Last modified June 16, 2009. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Aspartokinase
    EC=2.7.2.4
Alternative name(s):
    Aspartate kinase
      Short name=ASK
Gene names
Name: ask
Synonyms: askAB
Ordered Locus Names: TT_C0166
OrganismThermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) [Complete proteome] [HAMAP]
Taxonomic identifier262724 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length405 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Catalytic activity

ATP + L-aspartate = ADP + 4-phospho-L-aspartate.

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; tetrahydrodipicolinate from L-aspartate: step 1/4.

Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3.

Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.

Subunit structure

Tetramer consisting of 2 isoforms Alpha (catalytic) and 2 isoforms Beta (function not known) By similarity.

Sequence similarities

Belongs to the aspartokinase family.

Contains 2 ACT domains.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform Alpha (identifier: P61488-1)

Also known as: Aspartokinase subunit alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Beta (identifier: P61488-2)

Also known as: Aspartokinase subunit beta;

The sequence of this isoform differs from the canonical sequence as follows:
     1-244: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 405405Aspartokinase
PRO_0000002387

Regions

Domain262 – 33574ACT 1
Domain343 – 40462ACT 2

Natural variations

Alternative sequence1 – 244244Missing in isoform Beta.
VSP_018664

Sequences

Sequence LengthMass (Da)Tools
Isoform Alpha (Aspartokinase subunit alpha) [UniParc].

Last modified May 24, 2004. Version 1.
Checksum: 06F7B79E908960EA

FASTA40543,333
        10         20         30         40         50         60 
MALVVQKYGG TSVGDLERIH KVAQRIAHYR EKGHRLAVVV SAMGHTTDEL IALAKRVNPR 

        70         80         90        100        110        120 
PPFRELDLLT TTGEQVSVAL LSMQLWAMGI PAKGFVQHQI GITTDGRYGD ARILEVNPAR 

       130        140        150        160        170        180 
IREALEQGFV AVIAGFMGTT PEGEITTLGR GGSDTTAVAI AAALGAKECE IYTDTEGVYT 

       190        200        210        220        230        240 
TDPHLIPEAR KLSVIGYDQM LEMAALGARV LHPRAVYYAK RYGVVLHVRS SFSYNPGTLV 

       250        260        270        280        290        300 
KEVAMEMDKA VTGVALDLDH AQIGLIGIPD QPGIAAKVFQ ALAERGIAVD MIIQGVPGHD 

       310        320        330        340        350        360 
PSRQQMAFTV KKDFAQEALE ALEPVLAEIG GEAILRPDIA KVSIVGVGLA STPEVPAKMF 

       370        380        390        400 
QAVASTGANI EMIATSEVRI SVIIPAEYAE AALRAVHQAF ELDKA 

« Hide

Isoform Beta (Aspartokinase subunit beta).

Checksum: 94BDE8A671CC54EA
Show »

FASTA16116,895

References

« Hide 'large scale' references
[1]"Aspartate kinase-independent lysine synthesis in an extremely thermophilic bacterium, Thermus thermophilus: lysine is synthesized via alpha-aminoadipic acid not via diaminopimelic acid."
Kobashi N., Nishiyama M., Tanokura M.
J. Bacteriol. 181:1713-1718(1999) [PubMed: 10074061] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The genome sequence of the extreme thermophile Thermus thermophilus."
Henne A., Brueggemann H., Raasch C., Wiezer A., Hartsch T., Liesegang H., Johann A., Lienard T., Gohl O., Martinez-Arias R., Jacobi C., Starkuviene V., Schlenczeck S., Dencker S., Huber R., Klenk H.-P., Kramer W., Merkl R., Gottschalk G., Fritz H.-J.
Nat. Biotechnol. 22:547-553(2004) [PubMed: 15064768] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

AB013131 Genomic DNA. Translation: BAA25848.1.
AB013131 Genomic DNA. Translation: BAA25849.1.
AE017221 Genomic DNA. Translation: AAS80514.1. Different initiation.
RefSeqYP_004141.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID2775578.
GenomeReviewsGene locus TT_C0166 in contig AE017221_GR.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMP61488.

Enzyme and pathway databases

BioCycTTHE262724:TT_C0166-MON.

Family and domain databases

InterProIPR002912. ACT_bd.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR005260. Asp_kin_monofn.
IPR001341. Asp_kin_reg.
IPR018042. Aspartate_kinase_CS.
[Graphical view]
Gene3DG3DSA:3.40.1160.10. Aa_kinase. 1 hit.
PfamPF00696. AA_kinase. 1 hit.
PF01842. ACT. 2 hits.
[Graphical view]
TIGRFAMsTIGR00656. asp_kin_monofn. 1 hit.
TIGR00657. asp_kinases. 1 hit.
PROSITEPS00324. ASPARTOKINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAK_THET2
AccessionPrimary (citable) accession number: P61488
Secondary accession number(s): P77991, P97151
Entry history
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 24, 2004
Last modified: June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents