Reviewed, UniProtKB/Swiss-Prot P61488 (AK_THET2)

Names and origin

Protein names

Recommended name:
    Aspartokinase
    EC=2.7.2.4
Alternative name(s):
    Aspartate kinase
      Short name=ASK

Gene names

Name:	ask
Synonyms:	askAB
Ordered Locus Names:	TT_C0166

Organism

Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) [Complete proteome] [HAMAP]

Taxonomic identifier

262724 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	405 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate = ADP + 4-phospho-L-aspartate.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; tetrahydrodipicolinate from L-aspartate: step 1/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.
Subunit structure	Tetramer consisting of 2 isoforms Alpha (catalytic) and 2 isoforms Beta (function not known) By similarity.
Sequence similarities	Belongs to the aspartokinase family. Contains 2 ACT domains.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Coding sequence diversity	Alternative initiation
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW amino acid binding Inferred from electronic annotation. Source: InterPro aspartate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform Alpha (identifier: P61488-1)

Also known as: Aspartokinase subunit alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Beta (identifier: P61488-2)

Also known as: Aspartokinase subunit beta;

The sequence of this isoform differs from the canonical sequence as follows:
     1-244: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 405	405	Aspartokinase		PRO_0000002387
Regions
	Domain	262 – 335	74	ACT 1
	Domain	343 – 404	62	ACT 2
Natural variations
	Alternative sequence	1 – 244	244	Missing in isoform Beta.		VSP_018664

Sequences

	Sequence		Length	Mass (Da)
	Isoform Alpha (Aspartokinase subunit alpha) [UniParc]. Last modified May 24, 2004. Version 1. Checksum: 06F7B79E908960EA	FASTA	405	43,333
	10 20 30 40 50 60 MALVVQKYGG TSVGDLERIH KVAQRIAHYR EKGHRLAVVV SAMGHTTDEL IALAKRVNPR 70 80 90 100 110 120 PPFRELDLLT TTGEQVSVAL LSMQLWAMGI PAKGFVQHQI GITTDGRYGD ARILEVNPAR 130 140 150 160 170 180 IREALEQGFV AVIAGFMGTT PEGEITTLGR GGSDTTAVAI AAALGAKECE IYTDTEGVYT 190 200 210 220 230 240 TDPHLIPEAR KLSVIGYDQM LEMAALGARV LHPRAVYYAK RYGVVLHVRS SFSYNPGTLV 250 260 270 280 290 300 KEVAMEMDKA VTGVALDLDH AQIGLIGIPD QPGIAAKVFQ ALAERGIAVD MIIQGVPGHD 310 320 330 340 350 360 PSRQQMAFTV KKDFAQEALE ALEPVLAEIG GEAILRPDIA KVSIVGVGLA STPEVPAKMF 370 380 390 400 QAVASTGANI EMIATSEVRI SVIIPAEYAE AALRAVHQAF ELDKA
	Isoform Beta (Aspartokinase subunit beta). Checksum: 94BDE8A671CC54EA	FASTA	161	16,895
	10 20 30 40 50 60 MEMDKAVTGV ALDLDHAQIG LIGIPDQPGI AAKVFQALAE RGIAVDMIIQ GVPGHDPSRQ 70 80 90 100 110 120 QMAFTVKKDF AQEALEALEP VLAEIGGEAI LRPDIAKVSI VGVGLASTPE VPAKMFQAVA 130 140 150 160 STGANIEMIA TSEVRISVII PAEYAEAALR AVHQAFELDK A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Aspartate kinase-independent lysine synthesis in an extremely thermophilic bacterium, Thermus thermophilus: lysine is synthesized via alpha-aminoadipic acid not via diaminopimelic acid." Kobashi N., Nishiyama M., Tanokura M. J. Bacteriol. 181:1713-1718(1999) [PubMed: 10074061] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of the extreme thermophile Thermus thermophilus." Henne A., Brueggemann H., Raasch C., Wiezer A., Hartsch T., Liesegang H., Johann A., Lienard T., Gohl O., Martinez-Arias R., Jacobi C., Starkuviene V., Schlenczeck S., Dencker S., Huber R., Klenk H.-P., Kramer W., Merkl R., Gottschalk G., Fritz H.-J. Nat. Biotechnol. 22:547-553(2004) [PubMed: 15064768] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB013131 Genomic DNA. Translation: BAA25848.1. AB013131 Genomic DNA. Translation: BAA25849.1. AE017221 Genomic DNA. Translation: AAS80514.1. Different initiation.
RefSeq	YP_004141.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2775578.
GenomeReviews	Gene locus TT_C0166 in contig AE017221_GR.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P61488.
Enzyme and pathway databases
BioCyc	TTHE262724:TT_C0166-MON.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR001048. Asp/Glu/Uridylate_kinase. IPR005260. Asp_kin_monofn. IPR001341. Asp_kin_reg. IPR018042. Aspartate_kinase_CS. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01842. ACT. 2 hits. [Graphical view]
TIGRFAMs	TIGR00656. asp_kin_monofn. 1 hit. TIGR00657. asp_kinases. 1 hit.
PROSITE	PS00324. ASPARTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AK_THET2

Accession

Primary (citable) accession number: P61488
Secondary accession number(s): P77991, P97151

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	May 24, 2004
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)