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Protein

DEP domain-containing protein 5

Gene

Depdc5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Names & Taxonomyi

Protein namesi
Recommended name:
DEP domain-containing protein 5
Gene namesi
Name:Depdc5
Synonyms:Kiaa0645
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2141101. Depdc5.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • Iml1 complex Source: MGI
  • lysosomal membrane Source: MGI
  • lysosome Source: MGI
  • perinuclear region of cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15911591DEP domain-containing protein 5PRO_0000079866Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei505 – 5051PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP61460.
MaxQBiP61460.
PaxDbiP61460.
PRIDEiP61460.

PTM databases

iPTMnetiP61460.
PhosphoSiteiP61460.

Expressioni

Tissue specificityi

Expressed at low levels in all brain regions. Expressed throughout brain development, including in midgestation embryonic head (11.5 dpc), neonatal brain and whole adult brain. Present in neurons and absent in non-neuronal cells, including astrocytes (at protein level).1 Publication

Gene expression databases

CleanExiMM_DEPDC5.
ExpressionAtlasiP61460. baseline and differential.
GenevisibleiP61460. MM.

Interactioni

Subunit structurei

Within the GATOR complex, component of the GATOR1 subcomplex, made of DEPDC5, NPRL2 and NPRL3. GATOR1 mediates the strong interaction of the GATOR complex with RRAGA/RRAGC and RRAGB/RRAGC heterodimers (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000113862.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1175 – 125076DEPPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the IML1 family.Curated
Contains 1 DEP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3572. Eukaryota.
ENOG410XQVG. LUCA.
GeneTreeiENSGT00390000016559.
HOGENOMiHOG000290718.
HOVERGENiHBG051337.
InParanoidiP61460.
OMAiHVTIKLT.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000591. DEP_dom.
IPR027244. IML1.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR13179. PTHR13179. 1 hit.
PfamiPF00610. DEP. 1 hit.
PF12257. DUF3608. 1 hit.
[Graphical view]
SMARTiSM00049. DEP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS50186. DEP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P61460-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRTTKVYKLV IHKKGFGGSD DELVVNPKVF PHIKLGDIVE IAHPNDEYSP
60 70 80 90 100
LLLQVKSLKE DLQKETISVD QTVTQVFRLR PYQDVYVNVV DPKDVTLDLV
110 120 130 140 150
ELTFKDQYIG RGDMWRLKKS LVSTCAYITQ KVEFAGIRAQ AGELWVKNEK
160 170 180 190 200
VMCGYISEET RVVFRSTSAM VYIFIQMSCE MWDFDIYGDL YFEKAVNGFL
210 220 230 240 250
ADLFTKWKEK NCSHEVTVVL FSRTFYDAKS IDEFPEINRA SIQEDHKGRF
260 270 280 290 300
YEDFYKVVVQ NERREEWTSL LVTIKKLFIQ YPVLVRLEQA GGFPQGDNST
310 320 330 340 350
SAQGNYLEAI NLSFNVFDKH YINRNFDRTG QMSVVITPGV GVFEVDRLLM
360 370 380 390 400
ILTKQRMIDN GIGVDLVCMG EQPLHAVPLF KLHNRSVPRD SRLGDDYNIP
410 420 430 440 450
HWINHSFYTS KSQLFCNSFT PRIKLAGKKS ASEKTKNGRD TSLGTPKESE
460 470 480 490 500
NTLPIQVDYD AYDAQVFRLP GPSRAQRLAT CRSVREQENH SRKSASSCDV
510 520 530 540 550
SSSPSLPSRA LPTEEVRSQA SDDSSLGKST NILMIPNPHL HQYEVSSSLG
560 570 580 590 600
YTSTRDVLEN MIEPPQRDSS APGRFHVGSA ESMLHVRPGG YTPQRALINP
610 620 630 640 650
FAPSRMPMKL TSNRRRWMHT FPVGPSGEAI QIHHQTRQNM AELQGSRQRD
660 670 680 690 700
PTHSSAELLE LAYHEAAGRH STSRQPGDSM SLNFSGTEEL SVSLLSNSST
710 720 730 740 750
GVNPRTQNKD SLEDSVSTSP DPMPGFCCTV GVDWKSLTTP ACLPLTTDYF
760 770 780 790 800
PDRQGLQNDY TEGCYDLLPE ADMDRRDEEG VQMTAQQVFE EFICQRLMQG
810 820 830 840 850
YQIIVQPKTQ KPNTTVPPPL SSSPLYSRGL VSRNRPEEEG QYWLSMGRTF
860 870 880 890 900
HKVTLKDKMI TVTRYLPKYP YESAQIHYTY SLCPSHSDSE FVSCWVDFCH
910 920 930 940 950
ERLEEYKWNY LDQYICSAGS EDFSLIESLK FWRTRFLLLP ACVTATKRIT
960 970 980 990 1000
EGEVHCDIYG DKPRADEDEW QLLDGFIRFV EGLNRIRRRH RSDRMIRKGT
1010 1020 1030 1040 1050
AMKGLQMTGP ISAHSLEAAG PPVGKKGTSA LSALLEMEAS QKSLGEQQTT
1060 1070 1080 1090 1100
VHGKSSTQPA ENSSVAMTPT YVDSPRKDGA FFMEFVRSPR TASSAFYPQA
1110 1120 1130 1140 1150
SVDQTAPLVL DSTSLGVSTG QPMDRGNNQT FGNSQNIEQA FPSANSGDYS
1160 1170 1180 1190 1200
SQQHVASSLT SSSTLVEILE AMKHPSTGVQ LLSEQKGLSP CCFISAEVVH
1210 1220 1230 1240 1250
WLMNNVEGVQ TQAMGIDIMQ KMLEEQLITH ASGEAWRTFI YGFYFYKIVM
1260 1270 1280 1290 1300
DKEPERVAMQ QPSAPWYTAG ADDFASFQRK WFEVAFVAEE LVHSEIPAFL
1310 1320 1330 1340 1350
LPWLPSRPAS YASRHSSFSR SFGGRSQAAA LLAATVPEQR TVTLDVDVNN
1360 1370 1380 1390 1400
RTDRLEWCSC YYHGNFSLNA AFEIKLHWMA VTATVLFEMV QGWHRKATSC
1410 1420 1430 1440 1450
GFLLVPVLEG PFALPSYLYG DPLRAQLFIP LNLSCLLKEG SEHLFDSFEP
1460 1470 1480 1490 1500
ETYWDRMHLF QEAIAHRFGF VQDKYSVSAF NFPAENKPQY IHVTGTVFLQ
1510 1520 1530 1540 1550
LPYSKRKFSG QQRRRRNSTS STNQNMFCEE RVGYNWAYNT MLTKTWRSSA
1560 1570 1580 1590
TGDEKFADRL LKDFTDFCIN RDNRLVTFWT NCLEKMHASA P
Length:1,591
Mass (Da):180,379
Last modified:May 29, 2013 - v2
Checksum:iB41891718FF11CBF
GO
Isoform 2 (identifier: P61460-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1078-1099: Missing.

Note: No experimental confirmation available.
Show »
Length:1,569
Mass (Da):177,885
Checksum:i49B5646E2E5B0365
GO

Sequence cautioni

The sequence BAC97990.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti106 – 16156DQYIG…SEETR → LCLYHSKSGICWHQ in BAC97990 (PubMed:14621295).CuratedAdd
BLAST
Sequence conflicti1018 – 10192AA → ST in BAC97990 (PubMed:14621295).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1078 – 109922Missing in isoform 2. 1 PublicationVSP_046542Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129180 Transcribed RNA. Translation: BAC97990.1. Different initiation.
AC108773 Genomic DNA. No translation available.
AC122203 Genomic DNA. No translation available.
CCDSiCCDS51463.1. [P61460-1]
RefSeqiNP_001164038.1. NM_001170567.1. [P61460-1]
XP_006504047.1. XM_006503984.2. [P61460-1]
XP_006504050.1. XM_006503987.2. [P61460-2]
UniGeneiMm.101524.

Genome annotation databases

EnsembliENSMUST00000119705; ENSMUSP00000113862; ENSMUSG00000037426. [P61460-1]
ENSMUST00000120902; ENSMUSP00000113980; ENSMUSG00000037426. [P61460-2]
GeneIDi277854.
KEGGimmu:277854.
UCSCiuc008xae.2. mouse. [P61460-1]
uc008xaf.2. mouse. [P61460-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129180 Transcribed RNA. Translation: BAC97990.1. Different initiation.
AC108773 Genomic DNA. No translation available.
AC122203 Genomic DNA. No translation available.
CCDSiCCDS51463.1. [P61460-1]
RefSeqiNP_001164038.1. NM_001170567.1. [P61460-1]
XP_006504047.1. XM_006503984.2. [P61460-1]
XP_006504050.1. XM_006503987.2. [P61460-2]
UniGeneiMm.101524.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000113862.

PTM databases

iPTMnetiP61460.
PhosphoSiteiP61460.

Proteomic databases

EPDiP61460.
MaxQBiP61460.
PaxDbiP61460.
PRIDEiP61460.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000119705; ENSMUSP00000113862; ENSMUSG00000037426. [P61460-1]
ENSMUST00000120902; ENSMUSP00000113980; ENSMUSG00000037426. [P61460-2]
GeneIDi277854.
KEGGimmu:277854.
UCSCiuc008xae.2. mouse. [P61460-1]
uc008xaf.2. mouse. [P61460-2]

Organism-specific databases

CTDi9681.
MGIiMGI:2141101. Depdc5.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG3572. Eukaryota.
ENOG410XQVG. LUCA.
GeneTreeiENSGT00390000016559.
HOGENOMiHOG000290718.
HOVERGENiHBG051337.
InParanoidiP61460.
OMAiHVTIKLT.

Miscellaneous databases

ChiTaRSiDepdc5. mouse.
PROiP61460.
SOURCEiSearch...

Gene expression databases

CleanExiMM_DEPDC5.
ExpressionAtlasiP61460. baseline and differential.
GenevisibleiP61460. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000591. DEP_dom.
IPR027244. IML1.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR13179. PTHR13179. 1 hit.
PfamiPF00610. DEP. 1 hit.
PF12257. DUF3608. 1 hit.
[Graphical view]
SMARTiSM00049. DEP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS50186. DEP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney and Testis.
  4. Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiDEPD5_MOUSE
AccessioniPrimary (citable) accession number: P61460
Secondary accession number(s): E9Q5J2, E9Q5Y0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 29, 2013
Last modified: July 6, 2016
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.