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Protein

Pterin-4-alpha-carbinolamine dehydratase

Gene

Pcbd1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in tetrahydrobiopterin biosynthesis. Seems to both prevent the formation of 7-pterins and accelerate the formation of quinonoid-BH2. Coactivator for HNF1A-dependent transcription. Regulates the dimerization of homeodomain protein HNF1A and enhances its transcriptional activity.2 Publications

Catalytic activityi

(6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4a-hydroxypterin = (6R)-6-(L-erythro-1,2-dihydroxypropyl)-7,8-dihydro-6H-pterin + H2O.2 Publications

GO - Molecular functioni

  • 4-alpha-hydroxytetrahydrobiopterin dehydratase activity Source: ProtInc
  • phenylalanine 4-monooxygenase activity Source: Ensembl
  • transcription coactivator activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Lyase

Keywords - Biological processi

Tetrahydrobiopterin biosynthesis, Transcription, Transcription regulation

Enzyme and pathway databases

BRENDAi4.2.1.96. 5301.
ReactomeiR-RNO-71182. Phenylalanine and tyrosine catabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Pterin-4-alpha-carbinolamine dehydratase (EC:4.2.1.96)
Short name:
PHS
Alternative name(s):
4-alpha-hydroxy-tetrahydropterin dehydratase
Dimerization cofactor of hepatocyte nuclear factor 1-alpha
Short name:
DCoH
Short name:
Dimerization cofactor of HNF1
Phenylalanine hydroxylase-stimulating protein
Pterin carbinolamine dehydratase
Short name:
PCD
Gene namesi
Name:Pcbd1
Synonyms:Dcoh, Pcbd
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 20

Organism-specific databases

RGDi3263. Pcbd1.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

  • Note: Cytoplasmic and/or nuclear.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • extracellular exosome Source: Ensembl
  • nucleoplasm Source: Ensembl
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000630542 – 104Pterin-4-alpha-carbinolamine dehydrataseAdd BLAST103

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP61459.
PRIDEiP61459.

PTM databases

iPTMnetiP61459.
PhosphoSitePlusiP61459.

Expressioni

Gene expression databases

BgeeiENSRNOG00000000566.
GenevisibleiP61459. RN.

Interactioni

Subunit structurei

Homotetramer and homodimer. Heterotetramer with HNF1A; formed by a dimer of dimers.5 Publications

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000687.

Structurei

Secondary structure

1104
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 16Combined sources7
Helixi18 – 21Combined sources4
Turni22 – 24Combined sources3
Beta strandi29 – 32Combined sources4
Beta strandi34 – 39Combined sources6
Helixi43 – 60Combined sources18
Beta strandi65 – 69Combined sources5
Beta strandi72 – 77Combined sources6
Turni80 – 83Combined sources4
Helixi87 – 102Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DCHX-ray3.00A/B/C/D/E/F/G/H1-104[»]
1DCOX-ray2.30A/B/C/D/E/F/G/H1-104[»]
1DCPX-ray2.30A/B/C/D/E/F/G/H1-104[»]
1F93X-ray2.60A/B/C/D1-104[»]
3HXAX-ray1.80A/B/C/D/E/F/G/H1-104[»]
ProteinModelPortaliP61459.
SMRiP61459.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61459.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni61 – 63Substrate binding3
Regioni78 – 81Substrate binding4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG4073. Eukaryota.
COG2154. LUCA.
GeneTreeiENSGT00390000007221.
HOGENOMiHOG000007680.
HOVERGENiHBG000259.
InParanoidiP61459.
KOiK01724.
OMAiIKAQEMD.
OrthoDBiEOG091G120V.
PhylomeDBiP61459.
TreeFamiTF300188.

Family and domain databases

Gene3Di3.30.1360.20. 1 hit.
HAMAPiMF_00434. Pterin_4_alpha. 1 hit.
InterProiIPR001533. Trans/pterin_deHydtase.
[Graphical view]
PANTHERiPTHR12599. PTHR12599. 1 hit.
PfamiPF01329. Pterin_4a. 1 hit.
[Graphical view]
SUPFAMiSSF55248. SSF55248. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61459-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGKAHRLSA EERDQLLPNL RAVGWNELEG RDAIFKQFHF KDFNRAFGFM
60 70 80 90 100
TRVALQAEKL DHHPEWFNVY NKVHITLSTH ECAGLSERDI NLASFIEQVA

VSMT
Length:104
Mass (Da):12,000
Last modified:January 23, 2007 - v2
Checksum:i997DF8C2417FE5F5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83740 mRNA. No translation available.
AJ005542 Genomic DNA. Translation: CAA06587.1.
L04537 mRNA. Translation: AAA40609.1.
PIRiA47189.
RefSeqiNP_001007602.1. NM_001007601.2.
XP_006256476.1. XM_006256414.1.
UniGeneiRn.81180.

Genome annotation databases

EnsembliENSRNOT00000000687; ENSRNOP00000000687; ENSRNOG00000000566.
GeneIDi29700.
KEGGirno:29700.
UCSCiRGD:3263. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83740 mRNA. No translation available.
AJ005542 Genomic DNA. Translation: CAA06587.1.
L04537 mRNA. Translation: AAA40609.1.
PIRiA47189.
RefSeqiNP_001007602.1. NM_001007601.2.
XP_006256476.1. XM_006256414.1.
UniGeneiRn.81180.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DCHX-ray3.00A/B/C/D/E/F/G/H1-104[»]
1DCOX-ray2.30A/B/C/D/E/F/G/H1-104[»]
1DCPX-ray2.30A/B/C/D/E/F/G/H1-104[»]
1F93X-ray2.60A/B/C/D1-104[»]
3HXAX-ray1.80A/B/C/D/E/F/G/H1-104[»]
ProteinModelPortaliP61459.
SMRiP61459.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000687.

PTM databases

iPTMnetiP61459.
PhosphoSitePlusiP61459.

Proteomic databases

PaxDbiP61459.
PRIDEiP61459.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000000687; ENSRNOP00000000687; ENSRNOG00000000566.
GeneIDi29700.
KEGGirno:29700.
UCSCiRGD:3263. rat.

Organism-specific databases

CTDi5092.
RGDi3263. Pcbd1.

Phylogenomic databases

eggNOGiKOG4073. Eukaryota.
COG2154. LUCA.
GeneTreeiENSGT00390000007221.
HOGENOMiHOG000007680.
HOVERGENiHBG000259.
InParanoidiP61459.
KOiK01724.
OMAiIKAQEMD.
OrthoDBiEOG091G120V.
PhylomeDBiP61459.
TreeFamiTF300188.

Enzyme and pathway databases

BRENDAi4.2.1.96. 5301.
ReactomeiR-RNO-71182. Phenylalanine and tyrosine catabolism.

Miscellaneous databases

EvolutionaryTraceiP61459.
PROiP61459.

Gene expression databases

BgeeiENSRNOG00000000566.
GenevisibleiP61459. RN.

Family and domain databases

Gene3Di3.30.1360.20. 1 hit.
HAMAPiMF_00434. Pterin_4_alpha. 1 hit.
InterProiIPR001533. Trans/pterin_deHydtase.
[Graphical view]
PANTHERiPTHR12599. PTHR12599. 1 hit.
PfamiPF01329. Pterin_4a. 1 hit.
[Graphical view]
SUPFAMiSSF55248. SSF55248. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPHS_RAT
AccessioniPrimary (citable) accession number: P61459
Secondary accession number(s): P70519, P80095, Q9D930
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.