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Protein

Pterin-4-alpha-carbinolamine dehydratase

Gene

Pcbd1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in tetrahydrobiopterin biosynthesis. Seems to both prevent the formation of 7-pterins and accelerate the formation of quinonoid-BH2. Coactivator for HNF1A-dependent transcription. Regulates the dimerization of homeodomain protein HNF1A and enhances its transcriptional activity (By similarity).By similarity

Catalytic activityi

(6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4a-hydroxypterin = (6R)-6-(L-erythro-1,2-dihydroxypropyl)-7,8-dihydro-6H-pterin + H2O.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Lyase

Keywords - Biological processi

Tetrahydrobiopterin biosynthesis, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-71182. Phenylalanine and tyrosine catabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Pterin-4-alpha-carbinolamine dehydratase (EC:4.2.1.96)
Short name:
PHS
Alternative name(s):
4-alpha-hydroxy-tetrahydropterin dehydratase
Dimerization cofactor of hepatocyte nuclear factor 1-alpha
Short name:
DCoH
Short name:
Dimerization cofactor of HNF1
Phenylalanine hydroxylase-stimulating protein
Pterin carbinolamine dehydratase
Short name:
PCD
Gene namesi
Name:Pcbd1
Synonyms:Dcoh, Pcbd
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:94873. Pcbd1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 104103Pterin-4-alpha-carbinolamine dehydratasePRO_0000063053Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP61458.
MaxQBiP61458.
PaxDbiP61458.
PeptideAtlasiP61458.
PRIDEiP61458.

PTM databases

iPTMnetiP61458.
PhosphoSiteiP61458.

Expressioni

Gene expression databases

BgeeiP61458.
GenevisibleiP61458. MM.

Interactioni

Subunit structurei

Homotetramer and homodimer. Heterotetramer with HNF1A; formed by a dimer of dimers (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199068. 18 interactions.
IntActiP61458. 5 interactions.
MINTiMINT-4107515.
STRINGi10090.ENSMUSP00000020298.

Structurei

3D structure databases

ProteinModelPortaliP61458.
SMRiP61458. Positions 2-104.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni61 – 633Substrate bindingBy similarity
Regioni78 – 814Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG4073. Eukaryota.
COG2154. LUCA.
GeneTreeiENSGT00390000007221.
HOGENOMiHOG000007680.
HOVERGENiHBG000259.
InParanoidiP61458.
KOiK01724.
OMAiIKAQEMD.
OrthoDBiEOG7034K1.
PhylomeDBiP61458.
TreeFamiTF300188.

Family and domain databases

Gene3Di3.30.1360.20. 1 hit.
HAMAPiMF_00434. Pterin_4_alpha.
InterProiIPR001533. Trans/pterin_deHydtase.
[Graphical view]
PANTHERiPTHR12599. PTHR12599. 1 hit.
PfamiPF01329. Pterin_4a. 1 hit.
[Graphical view]
SUPFAMiSSF55248. SSF55248. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61458-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGKAHRLSA EERDQLLPNL RAVGWNEVEG RDAIFKQFHF KDFNRAFGFM
60 70 80 90 100
TRVALQAEKL DHHPEWFNVY NKVHITLSTH ECAGLSERDI NLASFIEQVA

VSMT
Length:104
Mass (Da):11,986
Last modified:January 23, 2007 - v2
Checksum:i13C798C25D9117E9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83741 mRNA. No translation available.
AK007401 mRNA. Translation: BAB25014.1.
BC024354 mRNA. Translation: AAH24354.1.
CCDSiCCDS35913.1.
RefSeqiNP_079549.1. NM_025273.4.
UniGeneiMm.39473.

Genome annotation databases

EnsembliENSMUST00000020298; ENSMUSP00000020298; ENSMUSG00000020098.
GeneIDi13180.
KEGGimmu:13180.
UCSCiuc007ffj.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83741 mRNA. No translation available.
AK007401 mRNA. Translation: BAB25014.1.
BC024354 mRNA. Translation: AAH24354.1.
CCDSiCCDS35913.1.
RefSeqiNP_079549.1. NM_025273.4.
UniGeneiMm.39473.

3D structure databases

ProteinModelPortaliP61458.
SMRiP61458. Positions 2-104.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199068. 18 interactions.
IntActiP61458. 5 interactions.
MINTiMINT-4107515.
STRINGi10090.ENSMUSP00000020298.

PTM databases

iPTMnetiP61458.
PhosphoSiteiP61458.

Proteomic databases

EPDiP61458.
MaxQBiP61458.
PaxDbiP61458.
PeptideAtlasiP61458.
PRIDEiP61458.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020298; ENSMUSP00000020298; ENSMUSG00000020098.
GeneIDi13180.
KEGGimmu:13180.
UCSCiuc007ffj.3. mouse.

Organism-specific databases

CTDi5092.
MGIiMGI:94873. Pcbd1.

Phylogenomic databases

eggNOGiKOG4073. Eukaryota.
COG2154. LUCA.
GeneTreeiENSGT00390000007221.
HOGENOMiHOG000007680.
HOVERGENiHBG000259.
InParanoidiP61458.
KOiK01724.
OMAiIKAQEMD.
OrthoDBiEOG7034K1.
PhylomeDBiP61458.
TreeFamiTF300188.

Enzyme and pathway databases

ReactomeiR-MMU-71182. Phenylalanine and tyrosine catabolism.

Miscellaneous databases

PROiP61458.
SOURCEiSearch...

Gene expression databases

BgeeiP61458.
GenevisibleiP61458. MM.

Family and domain databases

Gene3Di3.30.1360.20. 1 hit.
HAMAPiMF_00434. Pterin_4_alpha.
InterProiIPR001533. Trans/pterin_deHydtase.
[Graphical view]
PANTHERiPTHR12599. PTHR12599. 1 hit.
PfamiPF01329. Pterin_4a. 1 hit.
[Graphical view]
SUPFAMiSSF55248. SSF55248. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a cofactor that regulates dimerization of a mammalian homeodomain protein."
    Mendel D.B., Khavari P.A., Conley P.B., Graves M.K., Hansen L.P., Admon A., Crabtree G.R.
    Science 254:1762-1767(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: C57BL/6 X CBA.
    Tissue: Liver.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Pancreas.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney, Liver and Pancreas.

Entry informationi

Entry nameiPHS_MOUSE
AccessioniPrimary (citable) accession number: P61458
Secondary accession number(s): P70519, P80095, Q9D930
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.