P61458 (PHS_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pterin-4-alpha-carbinolamine dehydratase
Short name=PHS
EC=4.2.1.96

Alternative name(s):
4-alpha-hydroxy-tetrahydropterin dehydratase
Dimerization cofactor of hepatocyte nuclear factor 1-alpha
Short name=DCoH
Short name=Dimerization cofactor of HNF1
Phenylalanine hydroxylase-stimulating protein
Pterin carbinolamine dehydratase
Short name=PCD

Gene names

Name:	Pcbd1
Synonyms:	Dcoh, Pcbd

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	104 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in tetrahydrobiopterin biosynthesis. Seems to both prevent the formation of 7-pterins and accelerate the formation of quinonoid-BH2. Coactivator for HNF1A-dependent transcription. Regulates the dimerization of homeodomain protein HNF1A and enhances its transcriptional activity By similarity.
Catalytic activity	(6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4a-hydroxypterin = (6R)-6-(L-erythro-1,2-dihydroxypropyl)-7,8-dihydro-6H-pterin + H2O.
Subunit structure	Homotetramer and homodimer. Heterotetramer with HNF1A; formed by a dimer of dimers By similarity.
Subcellular location	Cytoplasm. Nucleus. Note: Cytoplasmic and/or nuclear.
Sequence similarities	Belongs to the pterin-4-alpha-carbinolamine dehydratase family.

Ontologies

Keywords
Biological process	Tetrahydrobiopterin biosynthesis Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Molecular function	Activator Lyase
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	positive regulation of transcription, DNA-dependent Inferred from direct assay Ref.1. Source: MGI protein heterooligomerization Inferred from physical interaction. Source: MGI protein homotetramerization Inferred from physical interaction. Source: MGI regulation of protein homodimerization activity Inferred from direct assay Ref.1. Source: MGI tetrahydrobiopterin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from physical interaction Ref.1. Source: MGI
Molecular function	4-alpha-hydroxytetrahydrobiopterin dehydratase activity Inferred from electronic annotation. Source: EC phenylalanine 4-monooxygenase activity Inferred from direct assay. Source: MGI transcription coactivator activity Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 104	103	Pterin-4-alpha-carbinolamine dehydratase		PRO_0000063053
Regions
	Region	61 – 63	3	Substrate binding By similarity
	Region	78 – 81	4	Substrate binding By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	41	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P61458 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 13C798C25D9117E9	FASTA	104	11,986
	10 20 30 40 50 60 MAGKAHRLSA EERDQLLPNL RAVGWNEVEG RDAIFKQFHF KDFNRAFGFM TRVALQAEKL 70 80 90 100 DHHPEWFNVY NKVHITLSTH ECAGLSERDI NLASFIEQVA VSMT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a cofactor that regulates dimerization of a mammalian homeodomain protein." Mendel D.B., Khavari P.A., Conley P.B., Graves M.K., Hansen L.P., Admon A., Crabtree G.R. Science 254:1762-1767(1991) [PubMed: 1763325] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: C57BL/6 X CBA. Tissue: Liver.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Pancreas.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M83741 mRNA. No translation available. AK007401 mRNA. Translation: BAB25014.1. BC024354 mRNA. Translation: AAH24354.1.
IPI	IPI00223800.
RefSeq	NP_079549.1. NM_025273.3.
UniGene	Mm.39473.
3D structure databases
ProteinModelPortal	P61458.
SMR	P61458. Positions 2-104.
ModBase	Search...
Protein-protein interaction databases
STRING	P61458.
PTM databases
PhosphoSite	P61458.
Proteomic databases
PRIDE	P61458.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000020298; ENSMUSP00000020298; ENSMUSG00000020098.
GeneID	13180.
KEGG	mmu:13180.
UCSC	uc007ffj.2. mouse.
Organism-specific databases
CTD	5092.
MGI	MGI:94873. Pcbd1.
Phylogenomic databases
eggNOG	roNOG16514.
GeneTree	ENSGT00390000007221.
HOGENOM	HBG705804.
HOVERGEN	HBG000259.
InParanoid	P61458.
OMA	SEERKTR.
OrthoDB	EOG4QNMXP.
PhylomeDB	P61458.
Gene expression databases
ArrayExpress	P61458.
Bgee	P61458.
Genevestigator	P61458.
GermOnline	ENSMUSG00000020098. Mus musculus.
Family and domain databases
InterPro	IPR001533. Trans/pterin_deHydtase. [Graphical view]
Gene3D	G3DSA:3.30.1360.20. Trans_pterinDh. 1 hit.
KO	K01724.
PANTHER	PTHR12599. Trans_pterinDh. 1 hit.
Pfam	PF01329. Pterin_4a. 1 hit. [Graphical view]
SUPFAM	SSF55248. Trans_pterinDh. 1 hit.
ProtoNet	Search...
Other
NextBio	283296.
SOURCE	Search...

Entry information

Entry name

PHS_MOUSE

Accession

Primary (citable) accession number: P61458
Secondary accession number(s): P70519, P80095, Q9D930

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program