Reviewed, UniProtKB/Swiss-Prot P61433 (MURB_BDEBA)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylenolpyruvoylglucosamine reductase
    EC=1.1.1.158
Alternative name(s):
    UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	Bd3233

Organism

Bdellovibrio bacteriovorus [Complete proteome] [HAMAP]

Taxonomic identifier

959 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Bdellovibrionales › Bdellovibrionaceae › Bdellovibrio

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00037
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity. HAMAP MF_00037
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm Probable HAMAP MF_00037.
Sequence similarities	Belongs to the murB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_0000179179
Regions
	Domain	17 – 188	172	FAD-binding PCMH-type
Sites
	Active site	164	1	By similarity
	Active site	237	1	Proton donor By similarity
	Active site	332	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P61433-1 [UniParc]. Last modified May 24, 2004. Version 1. Checksum: FA5352363B3F713A	FASTA	336	38,105
	10 20 30 40 50 60 MQIQNSHDLS SSNTLQLRSL AERFVELYTP ADLSTLLMNP ELKNLPWKIL GGGSNLVLPS 70 80 90 100 110 120 QIEGLVLKVS NLGRKLIHED PDYWFVKAQA GEVWNDFVQW TLEEGYWGLE NLSLIPGTVG 130 140 150 160 170 180 AAPIQNIGAY GVEVKDTLWE VHALDLQTGE PRVFSNKECQ FSYRDSYFKQ EGAGRYLIWD 190 200 210 220 230 240 VTFRLPKKNV LHLEYGDIRK EVERNNWPQD PRHVAQAVIN IRQSKLPDPK VIGNAGSFFK 250 260 270 280 290 300 NPIVSKELRD GLLSKHNDLV SFPYEDRYKL AAGWLIDRAG WKGKKLGPVG MYEKQALVLV 310 320 330 NHGGATADDV WKLARQVSSD VHTQFGVEIE AEPIRW

References

[1]

"A predator unmasked: life cycle of Bdellovibrio bacteriovorus from a genomic perspective." Rendulic S., Jagtap P., Rosinus A., Eppinger M., Baar C., Lanz C., Keller H., Lambert C., Evans K.J., Goesmann A., Meyer F., Sockett R.E., Schuster S.C. Science 303:689-692(2004) [PubMed: 14752164] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15356 / HD100 / DSM 50701 / NCIB 9529.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842655 Genomic DNA. Translation: CAE78050.1.
RefSeq	NP_969991.1.
3D structure databases
SMR	P61433. Positions 7-334.
ModBase	Search...
Genome annotation databases
GeneID	2736854.
KEGG	bba:Bd3233.
NMPDR	fig|264462.1.peg.2964.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG686573.
OMA	WLIDQCG.
PhylomeDB	P61433.
Enzyme and pathway databases
BioCyc	BBAC264462:BD3233-MONOMER.
BRENDA	1.1.1.158. 3459.
Family and domain databases
HAMAP	MF_00037. MurB. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00179. murB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_BDEBA

Accession

Primary (citable) accession number: P61433

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	May 24, 2004
Last modified:	February 9, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)