Reviewed, UniProtKB/Swiss-Prot P61429 (PHNS2_DESVH)

Names and origin

Protein names

Recommended name:
    Periplasmic [NiFe] hydrogenase small subunit 2
    EC=1.12.2.1
Alternative name(s):
    NiFe hydrogenlyase small chain 2

Gene names

Name:	hynB2
Synonyms:	hynB-2
Ordered Locus Names:	DVU_2525

Organism

Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / NCIMB 8303) [Complete proteome] [HAMAP]

Taxonomic identifier

882 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Desulfovibrio

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 H2 + ferricytochrome c3 = 4 H+ + ferrocytochrome c3.
Cofactor	Binds 1 3Fe-4S cluster By similarity. Binds 2 4Fe-4S clusters By similarity.
Subunit structure	Heterodimer of a large and a small subunit By similarity.
Subcellular location	Periplasm By similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ferredoxin hydrogenase complex Inferred from electronic annotation. Source: InterPro periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro cytochrome-c3 hydrogenase activity Inferred from electronic annotation. Source: EC ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 49	49	Tat-type signal Potential
	Chain	50 – 324	275	Periplasmic [NiFe] hydrogenase small subunit 2		PRO_0000013418
Sites
	Metal binding	67	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	70	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	164	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	200	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	237	1	Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity
	Metal binding	240	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	265	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	271	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	280	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	298	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	301	1	Iron-sulfur 3 (3Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P61429-1 [UniParc]. Last modified May 24, 2004. Version 1. Checksum: B725F966C4D57052	FASTA	324	34,597
	10 20 30 40 50 60 MSYTTRIPAK LLARQAEDGR MTRREFMKFC GIVAVAMGMG PGFAPAVAEA LQAKGRPSVV 70 80 90 100 110 120 YMHGAECTGC TEGLLRSIDP FIDILMMEVI SLDYCETVMA AAGRAAHHAL EDALRNPAGY 130 140 150 160 170 180 VCTIEGAIPT RKGGVYGQVG GETMLSLFSR VASGAKAVIA MGTCASFGGI QAAAPNPSGA 190 200 210 220 230 240 IGVREALAPF GIQPINIAGC PPNPVNYIGT VVHLLTKGMP ELDSAGRPKM FYGTTVHDQC 250 260 270 280 290 300 ERRKHFNADE FAPGFESKEA REGWCLHKLG CRGPYTYNNC PTAQFNQVNW PVRAGAPCIG 310 320 CSEPGFWDAL APFNKDVRQK SDKA

References

[1]

"The genome sequence of the anaerobic, sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough." Heidelberg J.F., Seshadri R., Haveman S.A., Hemme C.L., Paulsen I.T., Kolonay J.F., Eisen J.A., Ward N.L., Methe B.A., Brinkac L.M., Daugherty S.C., DeBoy R.T., Dodson R.J., Durkin A.S., Madupu R., Nelson W.C., Sullivan S.A., Fouts D.E. , Haft D.H., Selengut J., Peterson J.D., Davidsen T.M., Zafar N., Zhou L., Radune D., Dimitrov G., Hance M., Tran K., Khouri H.M., Gill J., Utterback T.R., Feldblyum T.V., Wall J.D., Voordouw G., Fraser C.M. Nat. Biotechnol. 22:554-559(2004) [PubMed: 15077118] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017285 Genomic DNA. Translation: AAS96997.1. Different initiation.
RefSeq	YP_011737.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2795615.
GenomeReviews	Gene locus DVU_2525 in contig AE017285_GR.
KEGG	dvu:DVU2525.
NMPDR	fig|882.1.peg.2512.
TIGR	DVU_2525.
Phylogenomic databases
HOGENOM	P61429.
Enzyme and pathway databases
BioCyc	DVUL882:DVU_2525-MON.
Family and domain databases
InterPro	IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR001821. NiFe_hydrogenase_ssu. IPR006311. Tat. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
PIRSF	PIRSF000310. NiFe_hyd_ssu. 1 hit.
PRINTS	PR00614. NIHGNASESMLL.
TIGRFAMs	TIGR00391. hydA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHNS2_DESVH

Accession

Primary (citable) accession number: P61429

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	May 24, 2004
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)