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P61422

- THIED_GEOSL

UniProt

P61422 - THIED_GEOSL

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Protein

Thiamine biosynthesis bifunctional protein ThiED

Gene
thiDE, GSU0605
Organism
Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) By similarity.UniRule annotation
Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P By similarity.UniRule annotation

Catalytic activityi

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate.UniRule annotation
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine = ADP + 4-amino-5-phosphonooxymethyl-2-methylpyrimidine.UniRule annotation
ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine.UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei82 – 821HMP-PP By similarity
Metal bindingi83 – 831Magnesium By similarity
Metal bindingi102 – 1021Magnesium By similarity
Binding sitei121 – 1211HMP-PP By similarity
Binding sitei150 – 1501HMP-PP By similarity
Binding sitei177 – 1771THZ-P; via amide nitrogen By similarity
Binding sitei266 – 2661Hydroxymethylpyrimidine By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydroxymethylpyrimidine kinase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
  4. phosphomethylpyrimidine kinase activity Source: UniProtKB-EC
  5. thiamine-phosphate diphosphorylase activity Source: UniProtKB-EC

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-KW
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciGSUL243231:GH27-611-MONOMER.
UniPathwayiUPA00060; UER00138.
UPA00060; UER00141.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine biosynthesis bifunctional protein ThiED
Including the following 2 domains:
Thiamine-phosphate synthase (EC:2.5.1.3)
Short name:
TMP-PPase
Short name:
TP synthase
Short name:
TPS
Alternative name(s):
Thiamine-phosphate pyrophosphorylase
Short name:
TMP pyrophosphorylase
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase (EC:2.7.1.49, EC:2.7.4.7)
Alternative name(s):
Hydroxymethylpyrimidine kinase
Short name:
HMP kinase
Hydroxymethylpyrimidine phosphate kinase
Short name:
HMP-P kinase
Short name:
HMP-phosphate kinase
Short name:
HMPP kinase
Gene namesi
Name:thiDE
Ordered Locus Names:GSU0605
OrganismiGeobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
Taxonomic identifieri243231 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter
ProteomesiUP000000577: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 490490Thiamine biosynthesis bifunctional protein ThiEDUniRule annotationPRO_0000192040Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243231.GSU0605.

Structurei

3D structure databases

ProteinModelPortaliP61422.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 213213Thiamine-phosphate synthaseUniRule annotationAdd
BLAST
Regioni50 – 545HMP-PP binding By similarity
Regioni147 – 1493THZ-P binding By similarity
Regioni197 – 1982THZ-P binding By similarity
Regioni229 – 490262Hydroxymethylpyrimidine/phosphomethylpyrimidine kinaseUniRule annotationAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the thiamine-phosphate synthase family.
In the C-terminal section; belongs to the ThiD family.

Phylogenomic databases

eggNOGiCOG0351.
HOGENOMiHOG000134175.
KOiK14153.
OMAiYLAQGEP.
OrthoDBiEOG6XWV53.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.40.1190.20. 1 hit.
HAMAPiMF_00097. TMP_synthase.
InterProiIPR013785. Aldolase_TIM.
IPR004399. HMP/HMP-P_kinase.
IPR013749. PM/HMP-P_kinase-1.
IPR029056. Ribokinase-like.
IPR022998. ThiaminP_synth_SF.
IPR003733. TMP_synthase.
[Graphical view]
PfamiPF08543. Phos_pyr_kin. 1 hit.
PF02581. TMP-TENI. 1 hit.
[Graphical view]
SUPFAMiSSF51391. SSF51391. 1 hit.
SSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00097. HMP-P_kinase. 1 hit.
TIGR00693. thiE. 1 hit.

Sequencei

Sequence statusi: Complete.

P61422-1 [UniParc]FASTAAdd to Basket

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MASNGHTLRL VINRDKHDSV IRGLYLVTDH DDNLIPRVEA AIDGGARVVQ    50
YRNKNQDRES RLALGLELRE LCRRRSIPFI VNDDLEMAVS LKADGLHLGQ 100
GDGDPREARR VLGPGKIIGV STHTLSEALE AQAAGVDYIG LGAMFPSRSK 150
EVEHVAGSEL LAAIRSSISI PIVAIGGITR DNGASVIDAG ADAVAVISAV 200
LSHPDPALAA TEIALLFNRR APFPRGSVLT VAGSDSGGGA GIQADLKTVT 250
LLGSYGSSVL TALTAQNTRG VSGIHGVPPA FVADQLDAVF SDIPVDVVKT 300
GMLFSAETIV AIAAKLTEYR RRMVVVDPVM VAKGGANLID RGAVSVLKER 350
LFPLAYLVTP NIPEAERLTG ANISDEESMR EAARRLHRLG ARNVLLKGGH 400
LLAGDSVDIL FDGAAFHRFV SPRILSKNTH GTGCTFASAI ATYLAQGDPL 450
REAIARAKRY ITAAIRLAQP LGRGHGPVNH ILAAEDVRDR 490
Length:490
Mass (Da):51,910
Last modified:May 24, 2004 - v1
Checksum:i924B153FCD5E83D4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017180 Genomic DNA. Translation: AAR33936.1.
RefSeqiNP_951663.1. NC_002939.5.

Genome annotation databases

EnsemblBacteriaiAAR33936; AAR33936; GSU0605.
GeneIDi2687129.
KEGGigsu:GSU0605.
PATRICi22023961. VBIGeoSul17553_0603.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017180 Genomic DNA. Translation: AAR33936.1 .
RefSeqi NP_951663.1. NC_002939.5.

3D structure databases

ProteinModelPortali P61422.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243231.GSU0605.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAR33936 ; AAR33936 ; GSU0605 .
GeneIDi 2687129.
KEGGi gsu:GSU0605.
PATRICi 22023961. VBIGeoSul17553_0603.

Phylogenomic databases

eggNOGi COG0351.
HOGENOMi HOG000134175.
KOi K14153.
OMAi YLAQGEP.
OrthoDBi EOG6XWV53.

Enzyme and pathway databases

UniPathwayi UPA00060 ; UER00138 .
UPA00060 ; UER00141 .
BioCyci GSUL243231:GH27-611-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
3.40.1190.20. 1 hit.
HAMAPi MF_00097. TMP_synthase.
InterProi IPR013785. Aldolase_TIM.
IPR004399. HMP/HMP-P_kinase.
IPR013749. PM/HMP-P_kinase-1.
IPR029056. Ribokinase-like.
IPR022998. ThiaminP_synth_SF.
IPR003733. TMP_synthase.
[Graphical view ]
Pfami PF08543. Phos_pyr_kin. 1 hit.
PF02581. TMP-TENI. 1 hit.
[Graphical view ]
SUPFAMi SSF51391. SSF51391. 1 hit.
SSF53613. SSF53613. 1 hit.
TIGRFAMsi TIGR00097. HMP-P_kinase. 1 hit.
TIGR00693. thiE. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51573 / DSM 12127 / PCA.

Entry informationi

Entry nameiTHIED_GEOSL
AccessioniPrimary (citable) accession number: P61422
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 24, 2004
Last modified: September 3, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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