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Protein

Thiamine biosynthesis bifunctional protein ThiED

Gene

thiDE

Organism
Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).By similarity
Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P.By similarity

Catalytic activityi

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate.
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine = ADP + 4-amino-5-phosphonooxymethyl-2-methylpyrimidine.
ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei82 – 821HMP-PPBy similarity
Metal bindingi83 – 831MagnesiumBy similarity
Metal bindingi102 – 1021MagnesiumBy similarity
Binding sitei121 – 1211HMP-PPBy similarity
Binding sitei150 – 1501HMP-PPBy similarity
Binding sitei177 – 1771THZ-P; via amide nitrogenBy similarity
Binding sitei266 – 2661HydroxymethylpyrimidineBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciGSUL243231:GH27-611-MONOMER.
UniPathwayiUPA00060; UER00138.
UPA00060; UER00141.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine biosynthesis bifunctional protein ThiED
Including the following 2 domains:
Thiamine-phosphate synthase (EC:2.5.1.3)
Short name:
TMP-PPase
Short name:
TP synthase
Short name:
TPS
Alternative name(s):
Thiamine-phosphate pyrophosphorylase
Short name:
TMP pyrophosphorylase
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase (EC:2.7.1.49, EC:2.7.4.7)
Alternative name(s):
Hydroxymethylpyrimidine kinase
Short name:
HMP kinase
Hydroxymethylpyrimidine phosphate kinase
Short name:
HMP-P kinase
Short name:
HMP-phosphate kinase
Short name:
HMPP kinase
Gene namesi
Name:thiDE
Ordered Locus Names:GSU0605
OrganismiGeobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
Taxonomic identifieri243231 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter
ProteomesiUP000000577 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 490490Thiamine biosynthesis bifunctional protein ThiEDPRO_0000192040Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243231.GSU0605.

Structurei

3D structure databases

ProteinModelPortaliP61422.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 213213Thiamine-phosphate synthaseAdd
BLAST
Regioni50 – 545HMP-PP bindingBy similarity
Regioni147 – 1493THZ-P bindingBy similarity
Regioni197 – 1982THZ-P bindingBy similarity
Regioni229 – 490262Hydroxymethylpyrimidine/phosphomethylpyrimidine kinaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the thiamine-phosphate synthase family.Curated
In the C-terminal section; belongs to the ThiD family.Curated

Phylogenomic databases

eggNOGiCOG0351.
HOGENOMiHOG000134175.
InParanoidiP61422.
KOiK14153.
OMAiCRINSEA.
OrthoDBiEOG6XWV53.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.40.1190.20. 1 hit.
HAMAPiMF_00097. TMP_synthase.
InterProiIPR013785. Aldolase_TIM.
IPR004399. HMP/HMP-P_kinase.
IPR013749. PM/HMP-P_kinase-1.
IPR029056. Ribokinase-like.
IPR022998. ThiaminP_synth_SF.
IPR003733. TMP_synthase.
[Graphical view]
PfamiPF08543. Phos_pyr_kin. 1 hit.
PF02581. TMP-TENI. 1 hit.
[Graphical view]
SUPFAMiSSF51391. SSF51391. 1 hit.
SSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00097. HMP-P_kinase. 1 hit.
TIGR00693. thiE. 1 hit.

Sequencei

Sequence statusi: Complete.

P61422-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASNGHTLRL VINRDKHDSV IRGLYLVTDH DDNLIPRVEA AIDGGARVVQ
60 70 80 90 100
YRNKNQDRES RLALGLELRE LCRRRSIPFI VNDDLEMAVS LKADGLHLGQ
110 120 130 140 150
GDGDPREARR VLGPGKIIGV STHTLSEALE AQAAGVDYIG LGAMFPSRSK
160 170 180 190 200
EVEHVAGSEL LAAIRSSISI PIVAIGGITR DNGASVIDAG ADAVAVISAV
210 220 230 240 250
LSHPDPALAA TEIALLFNRR APFPRGSVLT VAGSDSGGGA GIQADLKTVT
260 270 280 290 300
LLGSYGSSVL TALTAQNTRG VSGIHGVPPA FVADQLDAVF SDIPVDVVKT
310 320 330 340 350
GMLFSAETIV AIAAKLTEYR RRMVVVDPVM VAKGGANLID RGAVSVLKER
360 370 380 390 400
LFPLAYLVTP NIPEAERLTG ANISDEESMR EAARRLHRLG ARNVLLKGGH
410 420 430 440 450
LLAGDSVDIL FDGAAFHRFV SPRILSKNTH GTGCTFASAI ATYLAQGDPL
460 470 480 490
REAIARAKRY ITAAIRLAQP LGRGHGPVNH ILAAEDVRDR
Length:490
Mass (Da):51,910
Last modified:May 24, 2004 - v1
Checksum:i924B153FCD5E83D4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017180 Genomic DNA. Translation: AAR33936.1.
RefSeqiNP_951663.1. NC_002939.5.
WP_010941267.1. NC_002939.5.

Genome annotation databases

EnsemblBacteriaiAAR33936; AAR33936; GSU0605.
GeneIDi2687129.
KEGGigsu:GSU0605.
PATRICi22023961. VBIGeoSul17553_0603.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017180 Genomic DNA. Translation: AAR33936.1.
RefSeqiNP_951663.1. NC_002939.5.
WP_010941267.1. NC_002939.5.

3D structure databases

ProteinModelPortaliP61422.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243231.GSU0605.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAR33936; AAR33936; GSU0605.
GeneIDi2687129.
KEGGigsu:GSU0605.
PATRICi22023961. VBIGeoSul17553_0603.

Phylogenomic databases

eggNOGiCOG0351.
HOGENOMiHOG000134175.
InParanoidiP61422.
KOiK14153.
OMAiCRINSEA.
OrthoDBiEOG6XWV53.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00138.
UPA00060; UER00141.
BioCyciGSUL243231:GH27-611-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.40.1190.20. 1 hit.
HAMAPiMF_00097. TMP_synthase.
InterProiIPR013785. Aldolase_TIM.
IPR004399. HMP/HMP-P_kinase.
IPR013749. PM/HMP-P_kinase-1.
IPR029056. Ribokinase-like.
IPR022998. ThiaminP_synth_SF.
IPR003733. TMP_synthase.
[Graphical view]
PfamiPF08543. Phos_pyr_kin. 1 hit.
PF02581. TMP-TENI. 1 hit.
[Graphical view]
SUPFAMiSSF51391. SSF51391. 1 hit.
SSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00097. HMP-P_kinase. 1 hit.
TIGR00693. thiE. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51573 / DSM 12127 / PCA.

Entry informationi

Entry nameiTHIED_GEOSL
AccessioniPrimary (citable) accession number: P61422
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 24, 2004
Last modified: May 27, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.