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Protein

Telomerase-binding protein EST1A

Gene

Smg6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. May have a general role in telomere regulation. Promotes in vitro the ability of TERT to elongate telomeres. Overexpression induces telomere uncapping, chromosomal end-to-end fusions (telomeric DNA persists at the fusion points) and did not perturb TRF2 telomeric localization. Binds to the single-stranded 5'-(GTGTGG)4GTGT-3' telomeric DNA, but not to a telomerase RNA template component (TER) (By similarity).By similarity
Plays a role in nonsense-mediated mRNA decay. Is thought to provide a link to the mRNA degradation machinery as it has endonuclease activity required to initiate NMD, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. Degrades single-stranded RNA (ssRNA), but not ssDNA or dsRNA (By similarity).By similarity

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi1250 – 12501Manganese; catalyticBy similarity
Metal bindingi1352 – 13521Manganese; catalyticBy similarity
Metal bindingi1391 – 13911Manganese; catalyticBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Nonsense-mediated mRNA decay

Keywords - Ligandi

DNA-binding, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
Telomerase-binding protein EST1A (EC:3.1.-.-)
Alternative name(s):
EST1-like protein A
Ever shorter telomeres 1A
Smg-6 homolog
Telomerase subunit EST1A
Gene namesi
Name:Smg6
Synonyms:Est1a, Kiaa0732
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2144117. Smg6.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Cytoplasm, Nucleus, Telomere

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14181418Telomerase-binding protein EST1APRO_0000087070Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei331 – 3311PhosphoserineCombined sources
Modified residuei470 – 4701PhosphothreonineCombined sources
Modified residuei478 – 4781PhosphothreonineBy similarity
Modified residuei483 – 4831PhosphoserineBy similarity
Modified residuei830 – 8301PhosphoserineBy similarity
Modified residuei869 – 8691PhosphoserineCombined sources
Modified residuei873 – 8731PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP61406.
MaxQBiP61406.
PaxDbiP61406.
PeptideAtlasiP61406.
PRIDEiP61406.

PTM databases

iPTMnetiP61406.
PhosphoSiteiP61406.

Expressioni

Gene expression databases

BgeeiENSMUSG00000038290.
ExpressionAtlasiP61406. baseline and differential.
GenevisibleiP61406. MM.

Interactioni

Subunit structurei

May form homooligomers. Component of the telomerase holoenzyme complex at least composed of TERT, DKC1, WRAP53/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and a telomerase RNA template component (TERC). Interacts with TERT, independently of the telomerase RNA. Interacts with SMG1, SMG5, SMG7, UPF1, UPF2, UPF3B and the PP2A catalytic subunits. Also interacts with the exon junction complex (EJC) composed at least of CASC3, EIF4A3, MAGOH and RBM8A; required for the process of nonsense-mediated mRNA decay (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043555.

Structurei

3D structure databases

ProteinModelPortaliP61406.
SMRiP61406. Positions 579-821, 868-1158, 1238-1416.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1245 – 1396152PINcAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni39 – 5921EJC-binding motif 1; mediates interaction with the EJCBy similarityAdd
BLAST
Regioni132 – 15221EJC-binding motif 2; mediates interaction with the EJCBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili566 – 60035Sequence analysisAdd
BLAST
Coiled coili1196 – 124348Sequence analysisAdd
BLAST

Domaini

The PINc domain confers endonuclease activity and is expected to bind the catalytic metal ion.By similarity

Sequence similaritiesi

Contains 1 PINc domain.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2162. Eukaryota.
ENOG410XR9Z. LUCA.
GeneTreeiENSGT00560000077120.
HOGENOMiHOG000112402.
HOVERGENiHBG051511.
InParanoidiP61406.
KOiK11124.
OMAiSRENMKE.
OrthoDBiEOG091G0417.
PhylomeDBiP61406.
TreeFamiTF327119.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
3.40.50.1010. 2 hits.
InterProiIPR018834. DNA/RNA-bd_Est1-type.
IPR019458. EST1.
IPR002716. PIN_dom.
IPR029060. PIN_domain-like.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF10374. EST1. 1 hit.
PF10373. EST1_DNA_bind. 1 hit.
PF13638. PIN_4. 1 hit.
[Graphical view]
SMARTiSM00670. PINc. 1 hit.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 2 hits.
SSF88723. SSF88723. 2 hits.

Sequencei

Sequence statusi: Complete.

P61406-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEGLERVRI SASELRGILA TLAPQAGSRE NMKELKEPRQ RKDNRRPDLE
60 70 80 90 100
IYKPGLSRLR NRPKTKEASG NEEFKDEIVN DRDSSAVGND TQLIQVCKEL
110 120 130 140 150
DSQQQNGPID AENSQAQETF PKTVGLEDRS LKIIKRSKKP DLQIYQPGRR
160 170 180 190 200
LQTITKESAG RADEEEILNQ VEQLRIEEDE CKGEAIKEEV NNKPDKTEIE
210 220 230 240 250
KHQSNDRVRT AKGEKGKKIE KGEGSKKVAD DSVPGKPGSV KRYSRSDKRR
260 270 280 290 300
NRYRTCSTSS AGSNNSAEGA GLTDNGCRRR RQDRAKERPR LKKQVSLSST
310 320 330 340 350
DSLDEDRVDE PDVLGSRRSS ERKKHLERNW SGCGEGEQKS NGKENRSALR
360 370 380 390 400
VTFDAETMSK DSPVVRSVKD NVDRMKSDKG PSSGGKGSEK QELRHPRQEL
410 420 430 440 450
RDRGRGILIL PAHTALSVSS SGSPESTPLG PRLLFGSGSK GSRSWGRGGT
460 470 480 490 500
TRRLWDPNNP DQKPALKSQT PQLHFLDTDD EISPTSWGDS RQAQASYYKF
510 520 530 540 550
QNSDNPYYYP RTPGPASQYP YAGYSPLQYP VGPTNGMYPG AYYPGYPAPS
560 570 580 590 600
GQYVCSPLPA STMSPEEIEQ HVRNMQQQEL HRLLRVADNQ ELQLSNLLSR
610 620 630 640 650
DRISTEGMEK MAQLRTELLQ LYERCILLDI EFSDSQNVDQ ILWKNAFYQV
660 670 680 690 700
IEKFRQLLKD PNSENPEQIR NRLLELLDEG SDFFDSLLQK LQVTYKFKLE
710 720 730 740 750
DYMDGLAIRS KPLRKTVKYA LISAQRSMIC QGDISRYREQ ANDTANYGKA
760 770 780 790 800
RSWYLKAQHI APKNGRPYNQ LALLAVYTRR KLDAVYYYMR SLAASNPILT
810 820 830 840 850
AKESLMSLFE ETKRKAEQME KKQHEEFDMS PDKWRKGKKS TFRHVGDDTT
860 870 880 890 900
RLEIWIHPSH SRSAQGTESG KDSEQENGLG SLSPSDLNKR FILSFLHAHG
910 920 930 940 950
KLFTRIGMET FPAVAEKVLK EFQVLLQHSP SPIGSTRMLQ LMTINMFAVH
960 970 980 990 1000
NSQLKDCFSE ECRSVIQEQA ASLGLAMFSL LVQRCTCLLK DSAKAQLSSP
1010 1020 1030 1040 1050
EDQEDQDDIK VSSFVPDLKE LLPSVKVWSD WMLGYPDTWN PPPTSLDLPL
1060 1070 1080 1090 1100
QVAVDVWSTL ADFCNILTAV NQSEVPLYKD PDDDLTLLIL EEDRLLSGFV
1110 1120 1130 1140 1150
PLLAAPQDPC YVEKTSDKVI AADCKRVTVL KYFLEALCGQ EEPLLAFKGG
1160 1170 1180 1190 1200
KYVSVAPVPD TMGKEMGSQE GKQLEDEEED VVIEDFEEDS EAEGSGGEDD
1210 1220 1230 1240 1250
IRELRAKKLA LARKIAEQQR RQEKIQAVLE DQSQMRQMEL EIRPLFLVPD
1260 1270 1280 1290 1300
TNGFIDHLAS LARLLESRKY ILVVPLIVIN ELDGLAKGQE TDHRAGGYAR
1310 1320 1330 1340 1350
VVQEKARKSI EFLERRFESR DSCLRALTSR GNELESIAFR SEDITGQLGN
1360 1370 1380 1390 1400
NDDLILSCCL HYCKDKAKDY MPTSKEEPIR LLREVVLLTD DRNLRVKALT
1410
RNVPVRDIPA FLTWAQVG
Length:1,418
Mass (Da):160,496
Last modified:May 24, 2004 - v1
Checksum:iDC48C12B38C6EB8A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL604066, AL603905, AL662892 Genomic DNA. Translation: CAI24256.1.
AL603905, AL604066, AL662892 Genomic DNA. Translation: CAI35093.1.
AL662892, AL603905, AL604066 Genomic DNA. Translation: CAI35928.1.
BC066040 mRNA. Translation: AAH66040.1.
AK129203 mRNA. Translation: BAC98013.1.
CCDSiCCDS25039.1.
RefSeqiNP_001002764.1. NM_001002764.1.
UniGeneiMm.288460.

Genome annotation databases

EnsembliENSMUST00000045281; ENSMUSP00000043555; ENSMUSG00000038290.
GeneIDi103677.
KEGGimmu:103677.
UCSCiuc007kcz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL604066, AL603905, AL662892 Genomic DNA. Translation: CAI24256.1.
AL603905, AL604066, AL662892 Genomic DNA. Translation: CAI35093.1.
AL662892, AL603905, AL604066 Genomic DNA. Translation: CAI35928.1.
BC066040 mRNA. Translation: AAH66040.1.
AK129203 mRNA. Translation: BAC98013.1.
CCDSiCCDS25039.1.
RefSeqiNP_001002764.1. NM_001002764.1.
UniGeneiMm.288460.

3D structure databases

ProteinModelPortaliP61406.
SMRiP61406. Positions 579-821, 868-1158, 1238-1416.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043555.

PTM databases

iPTMnetiP61406.
PhosphoSiteiP61406.

Proteomic databases

EPDiP61406.
MaxQBiP61406.
PaxDbiP61406.
PeptideAtlasiP61406.
PRIDEiP61406.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045281; ENSMUSP00000043555; ENSMUSG00000038290.
GeneIDi103677.
KEGGimmu:103677.
UCSCiuc007kcz.1. mouse.

Organism-specific databases

CTDi23293.
MGIiMGI:2144117. Smg6.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG2162. Eukaryota.
ENOG410XR9Z. LUCA.
GeneTreeiENSGT00560000077120.
HOGENOMiHOG000112402.
HOVERGENiHBG051511.
InParanoidiP61406.
KOiK11124.
OMAiSRENMKE.
OrthoDBiEOG091G0417.
PhylomeDBiP61406.
TreeFamiTF327119.

Enzyme and pathway databases

ReactomeiR-MMU-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

ChiTaRSiSmg6. mouse.
PROiP61406.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038290.
ExpressionAtlasiP61406. baseline and differential.
GenevisibleiP61406. MM.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
3.40.50.1010. 2 hits.
InterProiIPR018834. DNA/RNA-bd_Est1-type.
IPR019458. EST1.
IPR002716. PIN_dom.
IPR029060. PIN_domain-like.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF10374. EST1. 1 hit.
PF10373. EST1_DNA_bind. 1 hit.
PF13638. PIN_4. 1 hit.
[Graphical view]
SMARTiSM00670. PINc. 1 hit.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 2 hits.
SSF88723. SSF88723. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiEST1A_MOUSE
AccessioniPrimary (citable) accession number: P61406
Secondary accession number(s): Q5NC64
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 24, 2004
Last modified: September 7, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.