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Protein

Insulin gene enhancer protein ISL-1

Gene

Isl1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Binds and regulates the promoters of the insulin, glucagon and somatostatin genes. Involved in the specificarion of motor neurons in cooperation with LHX3 and LDB1 (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi181 – 24060HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin gene enhancer protein ISL-1
Short name:
Islet-1
Gene namesi
Name:Isl1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi61957. Isl1.

Subcellular locationi

GO - Cellular componenti

  • nucleoplasm Source: Ensembl
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 349349Insulin gene enhancer protein ISL-1PRO_0000075749Add
BLAST

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP61374.
PRIDEiP61374.

PTM databases

PhosphoSiteiP61374.

Expressioni

Gene expression databases

GenevisibleiP61374. RN.

Interactioni

Subunit structurei

At neuronal promoters, displaces LDB1 from LHX3 LIM domain to form a ternary complex in which ISL1 contacts both LHX3 and LDB1.By similarity

GO - Molecular functioni

  • estrogen receptor binding Source: BHF-UCL
  • ligand-dependent nuclear receptor binding Source: BHF-UCL

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017305.

Structurei

3D structure databases

ProteinModelPortaliP61374.
SMRiP61374. Positions 13-168, 180-244, 254-288.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 7054LIM zinc-binding 1PROSITE-ProRule annotationAdd
BLAST
Domaini79 – 13355LIM zinc-binding 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni262 – 29130LIM-binding domain (LID)By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi241 – 349109Gln-richAdd
BLAST

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 2 LIM zinc-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox, LIM domain, Repeat

Phylogenomic databases

eggNOGiKOG0490. Eukaryota.
ENOG410YIJ3. LUCA.
GeneTreeiENSGT00760000118921.
HOGENOMiHOG000236304.
HOVERGENiHBG004671.
InParanoidiP61374.
KOiK09370.
OMAiTDMGDMG.
OrthoDBiEOG7JQBPW.
PhylomeDBiP61374.
TreeFamiTF315442.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
2.10.110.10. 2 hits.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00412. LIM. 2 hits.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
SM00132. LIM. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Alpha (identifier: P61374-1) [UniParc]FASTAAdd to basket

Also known as: Isl1-alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGDMGDPPKK KRLISLCVGC GNQIHDQYIL RVSPDLEWHA ACLKCAECNQ
60 70 80 90 100
YLDESCTCFV RDGKTYCKRD YIRLYGIKCA KCSIGFSKND FVMRARSKVY
110 120 130 140 150
HIECFRCVAC SRQLIPGDEF ALREDGLFCR ADHDVVERAS LGAGDPLSPL
160 170 180 190 200
HPARPLQMAA EPISARQPAL RPHVHKQPEK TTRVRTVLNE KQLHTLRTCY
210 220 230 240 250
AANPRPDALM KEQLVEMTGL SPRVIRVWFQ NKRCKDKKRS IMMKQLQQQQ
260 270 280 290 300
PNDKTNIQGM TGTPMVAASP ERHDGGLQAN PVEVQSYQPP WKVLSDFALQ
310 320 330 340
SDIDQPAFQQ LVNFSEGGPG SNSTGSEVAS MSSQLPDTPN SMVASPIEA
Length:349
Mass (Da):39,036
Last modified:May 24, 2004 - v1
Checksum:i4DB82FD09154F404
GO
Isoform Beta (identifier: P61374-2) [UniParc]FASTAAdd to basket

Also known as: Isl1-beta

The sequence of this isoform differs from the canonical sequence as follows:
     256-278: Missing.

Show »
Length:326
Mass (Da):36,686
Checksum:i226972F6D190C598
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti67 – 671C → S in AAS46773 (Ref. 2) Curated
Sequence conflicti199 – 1991C → R in AAS46773 (Ref. 2) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei256 – 27823Missing in isoform Beta. 1 PublicationVSP_010339Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S69329 mRNA. Translation: AAB30128.1.
AY557632 mRNA. Translation: AAS46773.1.
X53258 mRNA. Translation: CAA37349.1.
PIRiI67417.
RefSeqiNP_059035.3. NM_017339.3. [P61374-1]
UniGeneiRn.36202.

Genome annotation databases

EnsembliENSRNOT00000017305; ENSRNOP00000017305; ENSRNOG00000012556. [P61374-1]
GeneIDi64444.
KEGGirno:64444.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S69329 mRNA. Translation: AAB30128.1.
AY557632 mRNA. Translation: AAS46773.1.
X53258 mRNA. Translation: CAA37349.1.
PIRiI67417.
RefSeqiNP_059035.3. NM_017339.3. [P61374-1]
UniGeneiRn.36202.

3D structure databases

ProteinModelPortaliP61374.
SMRiP61374. Positions 13-168, 180-244, 254-288.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017305.

PTM databases

PhosphoSiteiP61374.

Proteomic databases

PaxDbiP61374.
PRIDEiP61374.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000017305; ENSRNOP00000017305; ENSRNOG00000012556. [P61374-1]
GeneIDi64444.
KEGGirno:64444.

Organism-specific databases

CTDi3670.
RGDi61957. Isl1.

Phylogenomic databases

eggNOGiKOG0490. Eukaryota.
ENOG410YIJ3. LUCA.
GeneTreeiENSGT00760000118921.
HOGENOMiHOG000236304.
HOVERGENiHBG004671.
InParanoidiP61374.
KOiK09370.
OMAiTDMGDMG.
OrthoDBiEOG7JQBPW.
PhylomeDBiP61374.
TreeFamiTF315442.

Miscellaneous databases

NextBioi613174.
PROiP61374.

Gene expression databases

GenevisibleiP61374. RN.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
2.10.110.10. 2 hits.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00412. LIM. 2 hits.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
SM00132. LIM. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The LIM domain homeobox gene isl-1: conservation of human, hamster, and rat complementary deoxyribonucleic acid sequences and expression in cell types of nonneuroendocrine lineage."
    Wang M., Drucker D.J.
    Endocrinology 134:1416-1422(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ALPHA).
    Tissue: Keratinocyte.
  2. "Variation of insulin gene enhancer binding protein Isl-1."
    Liu J., Chen D., Zheng Y., Meng X., Liu Y.
    Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA).
    Strain: Wistar.
  3. "Insulin gene enhancer binding protein Isl-1 is a member of a novel class of proteins containing both a homeo- and a Cys-His domain."
    Karlsson O., Thor S., Norberg T., Ohlsson H., Edlund T.
    Nature 344:879-882(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-316 (ISOFORM ALPHA).

Entry informationi

Entry nameiISL1_RAT
AccessioniPrimary (citable) accession number: P61374
Secondary accession number(s): P20663, P47894
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 24, 2004
Last modified: May 11, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.