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Protein

Insulin gene enhancer protein ISL-1

Gene

Isl1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-binding transcriptional activator. Recognizes and binds to the consensus octamer binding site 5'-ATAATTAA-3' in promoter of target genes. Plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. Cooperates with the transcription factor POU4F2 to achieve maximal levels of expression of RGC target genes and RGC fate specification in the developing retina. Involved in the specification of motor neurons in cooperation with LHX3 and LDB1 (By similarity). Binds to insulin gene enhancer sequences (PubMed:1691825).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi181 – 240HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processDifferentiation, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin gene enhancer protein ISL-1
Short name:
Islet-1
Gene namesi
Name:Isl1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi61957. Isl1.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

  • nucleoplasm Source: Ensembl
  • nucleus Source: RGD

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000757491 – 349Insulin gene enhancer protein ISL-1Add BLAST349

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP61374.

PTM databases

PhosphoSitePlusiP61374.

Expressioni

Tissue specificityi

Expressed in islet cells of the pancreas (PubMed:7907017, PubMed:1691825).2 Publications

Gene expression databases

GenevisibleiP61374. RN.

Interactioni

Subunit structurei

At neuronal promoters, displaces LDB1 from LHX3 LIM domain to form a ternary complex in which ISL1 contacts both LHX3 and LDB1. Interacts (via C-terminus) with POU4F2 (via C-terminus). Interacts with POU3F2. Interacts with POU4F3.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017305.

Structurei

3D structure databases

ProteinModelPortaliP61374.
SMRiP61374.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 70LIM zinc-binding 1PROSITE-ProRule annotationAdd BLAST54
Domaini79 – 133LIM zinc-binding 2PROSITE-ProRule annotationAdd BLAST55

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni262 – 291LIM-binding domain (LID)By similarityAdd BLAST30

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi241 – 349Gln-richAdd BLAST109

Keywords - Domaini

Homeobox, LIM domain, Repeat

Phylogenomic databases

eggNOGiKOG0490. Eukaryota.
ENOG410YIJ3. LUCA.
GeneTreeiENSGT00760000118921.
HOGENOMiHOG000236304.
HOVERGENiHBG004671.
InParanoidiP61374.
KOiK09370.
OMAiRIHDQWI.
OrthoDBiEOG091G09L9.
PhylomeDBiP61374.
TreeFamiTF315442.

Family and domain databases

InterProiView protein in InterPro
IPR009057. Homeobox-like.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR001781. Znf_LIM.
PfamiView protein in Pfam
PF00046. Homeobox. 1 hit.
PF00412. LIM. 2 hits.
SMARTiView protein in SMART
SM00389. HOX. 1 hit.
SM00132. LIM. 2 hits.
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiView protein in PROSITE
PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P61374-1) [UniParc]FASTAAdd to basket
Also known as: Isl1-alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGDMGDPPKK KRLISLCVGC GNQIHDQYIL RVSPDLEWHA ACLKCAECNQ
60 70 80 90 100
YLDESCTCFV RDGKTYCKRD YIRLYGIKCA KCSIGFSKND FVMRARSKVY
110 120 130 140 150
HIECFRCVAC SRQLIPGDEF ALREDGLFCR ADHDVVERAS LGAGDPLSPL
160 170 180 190 200
HPARPLQMAA EPISARQPAL RPHVHKQPEK TTRVRTVLNE KQLHTLRTCY
210 220 230 240 250
AANPRPDALM KEQLVEMTGL SPRVIRVWFQ NKRCKDKKRS IMMKQLQQQQ
260 270 280 290 300
PNDKTNIQGM TGTPMVAASP ERHDGGLQAN PVEVQSYQPP WKVLSDFALQ
310 320 330 340
SDIDQPAFQQ LVNFSEGGPG SNSTGSEVAS MSSQLPDTPN SMVASPIEA
Length:349
Mass (Da):39,036
Last modified:May 24, 2004 - v1
Checksum:i4DB82FD09154F404
GO
Isoform 2 (identifier: P61374-2) [UniParc]FASTAAdd to basket
Also known as: Isl1-beta

The sequence of this isoform differs from the canonical sequence as follows:
     256-278: Missing.

Show »
Length:326
Mass (Da):36,686
Checksum:i226972F6D190C598
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti67C → S in AAS46773 (Ref. 2) Curated1
Sequence conflicti199C → R in AAS46773 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010339256 – 278Missing in isoform 2. 1 PublicationAdd BLAST23

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S69329 mRNA. Translation: AAB30128.1.
AY557632 mRNA. Translation: AAS46773.1.
X53258 mRNA. Translation: CAA37349.1.
PIRiI67417.
RefSeqiNP_059035.3. NM_017339.3. [P61374-1]
UniGeneiRn.36202.

Genome annotation databases

EnsembliENSRNOT00000017305; ENSRNOP00000017305; ENSRNOG00000012556. [P61374-1]
GeneIDi64444.
KEGGirno:64444.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiISL1_RAT
AccessioniPrimary (citable) accession number: P61374
Secondary accession number(s): P20663, P47894
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 24, 2004
Last modified: July 5, 2017
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome