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P61371 (ISL1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Insulin gene enhancer protein ISL-1

Short name=Islet-1
Gene names
Name:ISL1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length349 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds and regulates the promoters of the insulin, glucagon and somatostatin genes. Involved in the specificarion of motor neurons in cooperation with LHX3 and LDB1 By similarity.

Subunit structure

At neuronal promoters, displaces LDB1 from LHX3 LIM domain to form a ternary complex in which ISL1 contacts both LHX3 and LDB1 By similarity.

Subcellular location

Nucleus.

Tissue specificity

Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain.

Post-translational modification

Phosphorylated By similarity.

Sequence similarities

Contains 1 homeobox DNA-binding domain.

Contains 2 LIM zinc-binding domains.

Ontologies

Keywords
   Cellular componentNucleus
   DomainHomeobox
LIM domain
Repeat
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionDevelopmental protein
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processatrial septum morphogenesis

Inferred from sequence or structural similarity. Source: BHF-UCL

axon regeneration

Inferred from electronic annotation. Source: Ensembl

cardiac cell fate determination

Inferred from direct assay PubMed 19571884. Source: BHF-UCL

cardiac muscle cell myoblast differentiation

Inferred from electronic annotation. Source: Ensembl

cardiac right ventricle morphogenesis

Inferred from sequence or structural similarity. Source: BHF-UCL

cellular response to glucocorticoid stimulus

Inferred from sequence or structural similarity. Source: BHF-UCL

endocardial cushion morphogenesis

Inferred from sequence or structural similarity. Source: BHF-UCL

innervation

Inferred from sequence or structural similarity. Source: BHF-UCL

mesenchymal cell differentiation

Inferred from sequence or structural similarity. Source: BHF-UCL

negative regulation of canonical Wnt signaling pathway

Inferred from electronic annotation. Source: Ensembl

negative regulation of inflammatory response

Inferred from sequence or structural similarity. Source: BHF-UCL

negative regulation of intracellular estrogen receptor signaling pathway

Inferred from sequence or structural similarity. Source: BHF-UCL

negative regulation of neuron apoptotic process

Inferred from sequence or structural similarity. Source: BHF-UCL

negative regulation of neuron differentiation

Inferred from electronic annotation. Source: Ensembl

negative regulation of protein homodimerization activity

Inferred from sequence or structural similarity. Source: BHF-UCL

negative regulation of transcription from RNA polymerase II promoter

Inferred from sequence or structural similarity. Source: BHF-UCL

neural crest cell migration

Inferred from electronic annotation. Source: Ensembl

neuron fate specification

Inferred from sequence or structural similarity. Source: BHF-UCL

outflow tract morphogenesis

Inferred from sequence or structural similarity. Source: BHF-UCL

outflow tract septum morphogenesis

Inferred from sequence or structural similarity. Source: BHF-UCL

pancreas development

Inferred from sequence or structural similarity. Source: BHF-UCL

peripheral nervous system neuron axonogenesis

Inferred from sequence or structural similarity. Source: BHF-UCL

pharyngeal system development

Inferred from sequence or structural similarity. Source: BHF-UCL

pituitary gland development

Inferred from electronic annotation. Source: Ensembl

positive regulation of DNA binding

Inferred from electronic annotation. Source: Ensembl

positive regulation of angiogenesis

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of granulocyte colony-stimulating factor production

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of granulocyte macrophage colony-stimulating factor production

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of insulin secretion

Inferred by curator PubMed 19619559. Source: BHF-UCL

positive regulation of interferon-gamma production

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of interleukin-1 alpha production

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of interleukin-1 beta production

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of interleukin-12 production

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of interleukin-6 production

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of macrophage colony-stimulating factor production

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of transcription from RNA polymerase II promoter

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of tumor necrosis factor production

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of tyrosine phosphorylation of Stat3 protein

Inferred from electronic annotation. Source: Ensembl

positive regulation vascular endothelial growth factor production

Inferred from sequence or structural similarity. Source: BHF-UCL

regulation of secondary heart field cardioblast proliferation

Inferred from electronic annotation. Source: Ensembl

retinal ganglion cell axon guidance

Inferred from electronic annotation. Source: Ensembl

secondary heart field specification

Inferred from mutant phenotype PubMed 20520780. Source: BHF-UCL

sensory system development

Inferred from sequence or structural similarity. Source: BHF-UCL

spinal cord motor neuron cell fate specification

Inferred from electronic annotation. Source: Ensembl

spinal cord motor neuron differentiation

Inferred from sequence or structural similarity. Source: BHF-UCL

trigeminal nerve development

Inferred from sequence or structural similarity. Source: BHF-UCL

ventricular cardiac muscle tissue morphogenesis

Inferred from sequence or structural similarity. Source: BHF-UCL

visceral motor neuron differentiation

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytoplasm

Non-traceable author statement Ref.1. Source: BHF-UCL

nucleus

Non-traceable author statement Ref.1. Source: BHF-UCL

   Molecular_functionRNA polymerase II activating transcription factor binding

Inferred from physical interaction PubMed 19619559. Source: BHF-UCL

RNA polymerase II transcription coactivator activity

Inferred from direct assay PubMed 19619559. Source: BHF-UCL

bHLH transcription factor binding

Inferred from physical interaction PubMed 16321656. Source: BHF-UCL

chromatin binding

Inferred from electronic annotation. Source: Ensembl

enhancer sequence-specific DNA binding

Inferred from direct assay PubMed 19619559. Source: BHF-UCL

estrogen receptor binding

Inferred from sequence or structural similarity. Source: BHF-UCL

ligand-dependent nuclear receptor binding

Inferred from sequence or structural similarity. Source: BHF-UCL

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 349349Insulin gene enhancer protein ISL-1
PRO_0000075746

Regions

Domain17 – 7054LIM zinc-binding 1
Domain79 – 13355LIM zinc-binding 2
DNA binding181 – 24060Homeobox
Region262 – 29130LIM-binding domain (LID) By similarity
Compositional bias241 – 349109Gln-rich

Sequences

Sequence LengthMass (Da)Tools
P61371 [UniParc].

Last modified May 24, 2004. Version 1.
Checksum: 4DB82FD09154F404

FASTA34939,036
        10         20         30         40         50         60 
MGDMGDPPKK KRLISLCVGC GNQIHDQYIL RVSPDLEWHA ACLKCAECNQ YLDESCTCFV 

        70         80         90        100        110        120 
RDGKTYCKRD YIRLYGIKCA KCSIGFSKND FVMRARSKVY HIECFRCVAC SRQLIPGDEF 

       130        140        150        160        170        180 
ALREDGLFCR ADHDVVERAS LGAGDPLSPL HPARPLQMAA EPISARQPAL RPHVHKQPEK 

       190        200        210        220        230        240 
TTRVRTVLNE KQLHTLRTCY AANPRPDALM KEQLVEMTGL SPRVIRVWFQ NKRCKDKKRS 

       250        260        270        280        290        300 
IMMKQLQQQQ PNDKTNIQGM TGTPMVAASP ERHDGGLQAN PVEVQSYQPP WKVLSDFALQ 

       310        320        330        340 
SDIDQPAFQQ LVNFSEGGPG SNSTGSEVAS MSSQLPDTPN SMVASPIEA 

« Hide

References

« Hide 'large scale' references
[1]"The LIM domain homeobox gene isl-1: conservation of human, hamster, and rat complementary deoxyribonucleic acid sequences and expression in cell types of nonneuroendocrine lineage."
Wang M., Drucker D.J.
Endocrinology 134:1416-1422(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Isolation of the human LIM/homeodomain gene islet-1 and identification of a simple sequence repeat polymorphism."
Tanizawa Y., Riggs A.C., Dagogo-Jack S., Vaxillaire M., Froguel P., Liu L., Donis-Keller H., Permutt M.A.
Diabetes 43:935-941(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-349.
Tissue: Pancreatic islet.
[3]Erratum
Tanizawa Y., Riggs A.C., Dagogo-Jack S., Vaxillaire M., Froguel P., Liu L., Donis-Keller H., Permutt M.A.
Diabetes 43:1171-1171(1994)
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[5]"Phosphoproteome of resting human platelets."
Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A.
J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Platelet.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S70721 mRNA. Translation: AAD14064.1.
U07559 mRNA. Translation: AAA81946.1.
BC031213 mRNA. Translation: AAH31213.1.
CCDSCCDS43314.1.
PIRI53277.
RefSeqNP_002193.2. NM_002202.2.
UniGeneHs.505.

3D structure databases

ProteinModelPortalP61371.
SMRP61371. Positions 13-168, 180-244, 254-288.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109877. 18 interactions.
IntActP61371. 5 interactions.
MINTMINT-3022667.
STRING9606.ENSP00000230658.

PTM databases

PhosphoSiteP61371.

Polymorphism databases

DMDM47606423.

Proteomic databases

PaxDbP61371.
PRIDEP61371.

Protocols and materials databases

DNASU3670.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000230658; ENSP00000230658; ENSG00000016082.
GeneID3670.
KEGGhsa:3670.
UCSCuc003jor.3. human.

Organism-specific databases

CTD3670.
GeneCardsGC05P050714.
HGNCHGNC:6132. ISL1.
HPAHPA057416.
MIM600366. gene.
neXtProtNX_P61371.
PharmGKBPA29932.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5576.
HOGENOMHOG000236304.
HOVERGENHBG004671.
InParanoidP61371.
KOK09370.
OMATDMGDMG.
OrthoDBEOG7JQBPW.
PhylomeDBP61371.
TreeFamTF315442.

Gene expression databases

ArrayExpressP61371.
BgeeP61371.
CleanExHS_ISL1.
GenevestigatorP61371.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
2.10.110.10. 2 hits.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR001781. Znf_LIM.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
PF00412. LIM. 2 hits.
[Graphical view]
SMARTSM00389. HOX. 1 hit.
SM00132. LIM. 2 hits.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiISL1.
GenomeRNAi3670.
NextBio14363.
PROP61371.
SOURCESearch...

Entry information

Entry nameISL1_HUMAN
AccessionPrimary (citable) accession number: P61371
Secondary accession number(s): P20663, P47894
Entry history
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 24, 2004
Last modified: July 9, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM