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Protein

Insulin gene enhancer protein ISL-1

Gene

ISL1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-binding transcriptional activator. Recognizes and binds to the consensus octamer binding site 5'-ATAATTAA-3' in promoter of target genes. Plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. Cooperates with the transcription factor POU4F2 to achieve maximal levels of expression of RGC target genes and RGC fate specification in the developing retina. Involved in the specification of motor neurons in cooperation with LHX3 and LDB1. Binds to insulin gene enhancer sequences.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi181 – 240HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processDifferentiation, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-400511. Synthesis, secretion, and inactivation of Glucose-dependent Insulinotropic Polypeptide (GIP).
SIGNORiP61371.

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin gene enhancer protein ISL-1
Short name:
Islet-1
Gene namesi
Name:ISL1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:6132. ISL1.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

  • cytoplasm Source: BHF-UCL
  • nucleoplasm Source: HPA
  • nucleus Source: BHF-UCL

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi3670.
MalaCardsiISL1.
OpenTargetsiENSG00000016082.
PharmGKBiPA29932.

Polymorphism and mutation databases

BioMutaiISL1.
DMDMi47606423.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000757461 – 349Insulin gene enhancer protein ISL-1Add BLAST349

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP61371.
MaxQBiP61371.
PaxDbiP61371.
PeptideAtlasiP61371.
PRIDEiP61371.

PTM databases

iPTMnetiP61371.
PhosphoSitePlusiP61371.

Expressioni

Tissue specificityi

Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain.1 Publication

Gene expression databases

BgeeiENSG00000016082.
CleanExiHS_ISL1.
ExpressionAtlasiP61371. baseline and differential.
GenevisibleiP61371. HS.

Organism-specific databases

HPAiHPA057416.

Interactioni

Subunit structurei

At neuronal promoters, displaces LDB1 from LHX3 LIM domain to form a ternary complex in which ISL1 contacts both LHX3 and LDB1. Interacts (via C-terminus) with POU4F2 (via C-terminus). Interacts with POU3F2. Interacts with POU4F3.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ZNF511Q8NB153EBI-3906896,EBI-10269136

GO - Molecular functioni

  • bHLH transcription factor binding Source: BHF-UCL
  • estrogen receptor binding Source: BHF-UCL
  • ligand-dependent nuclear receptor binding Source: BHF-UCL
  • RNA polymerase II activating transcription factor binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi109877. 27 interactors.
IntActiP61371. 10 interactors.
MINTiMINT-3022667.
STRINGi9606.ENSP00000230658.

Structurei

3D structure databases

ProteinModelPortaliP61371.
SMRiP61371.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 70LIM zinc-binding 1PROSITE-ProRule annotationAdd BLAST54
Domaini79 – 133LIM zinc-binding 2PROSITE-ProRule annotationAdd BLAST55

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni262 – 291LIM-binding domain (LID)By similarityAdd BLAST30

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi241 – 349Gln-richAdd BLAST109

Keywords - Domaini

Homeobox, LIM domain, Repeat

Phylogenomic databases

eggNOGiKOG0490. Eukaryota.
ENOG410YIJ3. LUCA.
GeneTreeiENSGT00760000118921.
HOGENOMiHOG000236304.
HOVERGENiHBG004671.
InParanoidiP61371.
KOiK09370.
OMAiRIHDQWI.
OrthoDBiEOG091G09L9.
PhylomeDBiP61371.
TreeFamiTF315442.

Family and domain databases

InterProiView protein in InterPro
IPR009057. Homeobox-like.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR001781. Znf_LIM.
PfamiView protein in Pfam
PF00046. Homeobox. 1 hit.
PF00412. LIM. 2 hits.
SMARTiView protein in SMART
SM00389. HOX. 1 hit.
SM00132. LIM. 2 hits.
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiView protein in PROSITE
PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 2 hits.

Sequencei

Sequence statusi: Complete.

P61371-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDMGDPPKK KRLISLCVGC GNQIHDQYIL RVSPDLEWHA ACLKCAECNQ
60 70 80 90 100
YLDESCTCFV RDGKTYCKRD YIRLYGIKCA KCSIGFSKND FVMRARSKVY
110 120 130 140 150
HIECFRCVAC SRQLIPGDEF ALREDGLFCR ADHDVVERAS LGAGDPLSPL
160 170 180 190 200
HPARPLQMAA EPISARQPAL RPHVHKQPEK TTRVRTVLNE KQLHTLRTCY
210 220 230 240 250
AANPRPDALM KEQLVEMTGL SPRVIRVWFQ NKRCKDKKRS IMMKQLQQQQ
260 270 280 290 300
PNDKTNIQGM TGTPMVAASP ERHDGGLQAN PVEVQSYQPP WKVLSDFALQ
310 320 330 340
SDIDQPAFQQ LVNFSEGGPG SNSTGSEVAS MSSQLPDTPN SMVASPIEA
Length:349
Mass (Da):39,036
Last modified:May 24, 2004 - v1
Checksum:i4DB82FD09154F404
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S70721 mRNA. Translation: AAD14064.1.
U07559 mRNA. Translation: AAA81946.1.
BC031213 mRNA. Translation: AAH31213.1.
CCDSiCCDS43314.1.
PIRiI53277.
RefSeqiNP_002193.2. NM_002202.2.
UniGeneiHs.505.

Genome annotation databases

EnsembliENST00000230658; ENSP00000230658; ENSG00000016082.
GeneIDi3670.
KEGGihsa:3670.
UCSCiuc003jor.4. human.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiISL1_HUMAN
AccessioniPrimary (citable) accession number: P61371
Secondary accession number(s): P20663, P47894
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 24, 2004
Last modified: July 5, 2017
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot