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Protein

Insulin gene enhancer protein ISL-1

Gene

ISL1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds and regulates the promoters of the insulin, glucagon and somatostatin genes. Involved in the specificarion of motor neurons in cooperation with LHX3 and LDB1 (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi181 – 24060HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_23824. Synthesis, secretion, and inactivation of Glucose-dependent Insulinotropic Polypeptide (GIP).

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin gene enhancer protein ISL-1
Short name:
Islet-1
Gene namesi
Name:ISL1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:6132. ISL1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: BHF-UCL
  • mitochondrion Source: HPA
  • nucleoplasm Source: HPA
  • nucleus Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29932.

Polymorphism and mutation databases

BioMutaiISL1.
DMDMi47606423.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 349349Insulin gene enhancer protein ISL-1PRO_0000075746Add
BLAST

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP61371.
PRIDEiP61371.

PTM databases

PhosphoSiteiP61371.

Expressioni

Tissue specificityi

Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain.

Gene expression databases

BgeeiP61371.
CleanExiHS_ISL1.
ExpressionAtlasiP61371. baseline and differential.
GenevisibleiP61371. HS.

Organism-specific databases

HPAiHPA057416.

Interactioni

Subunit structurei

At neuronal promoters, displaces LDB1 from LHX3 LIM domain to form a ternary complex in which ISL1 contacts both LHX3 and LDB1.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ZNF511Q8NB153EBI-3906896,EBI-10269136

Protein-protein interaction databases

BioGridi109877. 22 interactions.
IntActiP61371. 8 interactions.
MINTiMINT-3022667.
STRINGi9606.ENSP00000230658.

Structurei

3D structure databases

ProteinModelPortaliP61371.
SMRiP61371. Positions 13-168, 180-244, 254-288.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 7054LIM zinc-binding 1PROSITE-ProRule annotationAdd
BLAST
Domaini79 – 13355LIM zinc-binding 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni262 – 29130LIM-binding domain (LID)By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi241 – 349109Gln-richAdd
BLAST

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 2 LIM zinc-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox, LIM domain, Repeat

Phylogenomic databases

eggNOGiCOG5576.
GeneTreeiENSGT00760000118921.
HOGENOMiHOG000236304.
HOVERGENiHBG004671.
InParanoidiP61371.
KOiK09370.
OMAiTDMGDMG.
OrthoDBiEOG7JQBPW.
PhylomeDBiP61371.
TreeFamiTF315442.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
2.10.110.10. 2 hits.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00412. LIM. 2 hits.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
SM00132. LIM. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P61371-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDMGDPPKK KRLISLCVGC GNQIHDQYIL RVSPDLEWHA ACLKCAECNQ
60 70 80 90 100
YLDESCTCFV RDGKTYCKRD YIRLYGIKCA KCSIGFSKND FVMRARSKVY
110 120 130 140 150
HIECFRCVAC SRQLIPGDEF ALREDGLFCR ADHDVVERAS LGAGDPLSPL
160 170 180 190 200
HPARPLQMAA EPISARQPAL RPHVHKQPEK TTRVRTVLNE KQLHTLRTCY
210 220 230 240 250
AANPRPDALM KEQLVEMTGL SPRVIRVWFQ NKRCKDKKRS IMMKQLQQQQ
260 270 280 290 300
PNDKTNIQGM TGTPMVAASP ERHDGGLQAN PVEVQSYQPP WKVLSDFALQ
310 320 330 340
SDIDQPAFQQ LVNFSEGGPG SNSTGSEVAS MSSQLPDTPN SMVASPIEA
Length:349
Mass (Da):39,036
Last modified:May 24, 2004 - v1
Checksum:i4DB82FD09154F404
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S70721 mRNA. Translation: AAD14064.1.
U07559 mRNA. Translation: AAA81946.1.
BC031213 mRNA. Translation: AAH31213.1.
CCDSiCCDS43314.1.
PIRiI53277.
RefSeqiNP_002193.2. NM_002202.2.
UniGeneiHs.505.

Genome annotation databases

EnsembliENST00000230658; ENSP00000230658; ENSG00000016082.
GeneIDi3670.
KEGGihsa:3670.
UCSCiuc003jor.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S70721 mRNA. Translation: AAD14064.1.
U07559 mRNA. Translation: AAA81946.1.
BC031213 mRNA. Translation: AAH31213.1.
CCDSiCCDS43314.1.
PIRiI53277.
RefSeqiNP_002193.2. NM_002202.2.
UniGeneiHs.505.

3D structure databases

ProteinModelPortaliP61371.
SMRiP61371. Positions 13-168, 180-244, 254-288.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109877. 22 interactions.
IntActiP61371. 8 interactions.
MINTiMINT-3022667.
STRINGi9606.ENSP00000230658.

PTM databases

PhosphoSiteiP61371.

Polymorphism and mutation databases

BioMutaiISL1.
DMDMi47606423.

Proteomic databases

PaxDbiP61371.
PRIDEiP61371.

Protocols and materials databases

DNASUi3670.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000230658; ENSP00000230658; ENSG00000016082.
GeneIDi3670.
KEGGihsa:3670.
UCSCiuc003jor.3. human.

Organism-specific databases

CTDi3670.
GeneCardsiGC05P050714.
HGNCiHGNC:6132. ISL1.
HPAiHPA057416.
MIMi600366. gene.
neXtProtiNX_P61371.
PharmGKBiPA29932.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5576.
GeneTreeiENSGT00760000118921.
HOGENOMiHOG000236304.
HOVERGENiHBG004671.
InParanoidiP61371.
KOiK09370.
OMAiTDMGDMG.
OrthoDBiEOG7JQBPW.
PhylomeDBiP61371.
TreeFamiTF315442.

Enzyme and pathway databases

ReactomeiREACT_23824. Synthesis, secretion, and inactivation of Glucose-dependent Insulinotropic Polypeptide (GIP).

Miscellaneous databases

ChiTaRSiISL1. human.
GeneWikiiISL1.
GenomeRNAii3670.
NextBioi14363.
PROiP61371.
SOURCEiSearch...

Gene expression databases

BgeeiP61371.
CleanExiHS_ISL1.
ExpressionAtlasiP61371. baseline and differential.
GenevisibleiP61371. HS.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
2.10.110.10. 2 hits.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00412. LIM. 2 hits.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
SM00132. LIM. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The LIM domain homeobox gene isl-1: conservation of human, hamster, and rat complementary deoxyribonucleic acid sequences and expression in cell types of nonneuroendocrine lineage."
    Wang M., Drucker D.J.
    Endocrinology 134:1416-1422(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Isolation of the human LIM/homeodomain gene islet-1 and identification of a simple sequence repeat polymorphism."
    Tanizawa Y., Riggs A.C., Dagogo-Jack S., Vaxillaire M., Froguel P., Liu L., Donis-Keller H., Permutt M.A.
    Diabetes 43:935-941(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-349.
    Tissue: Pancreatic islet.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Platelet.

Entry informationi

Entry nameiISL1_HUMAN
AccessioniPrimary (citable) accession number: P61371
Secondary accession number(s): P20663, P47894
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 24, 2004
Last modified: July 22, 2015
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.