P61328 (FGF12_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fibroblast growth factor 12
Short name=FGF-12

Alternative name(s):
Fibroblast growth factor homologous factor 1
Short name=FHF-1
Myocyte-activating factor

Gene names

Name:	FGF12
Synonyms:	FGF12B, FHF1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably involved in nervous system development and function.
Subunit structure	Interacts with the C-terminal region of SCN9A By similarity.
Subcellular location	Nucleus Probable.
Tissue specificity	Brain, eye and testis; highly expressed in embryonic retina, olfactory epithelium, olfactory bulb, and in a segmental pattern of the body wall; in adult olfactory bulb, less in cerebellum, deep cerebellar nuclei, cortex and multiple midbrain structures.
Sequence similarities	Belongs to the heparin-binding growth factors family.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Growth factor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	JNK cascade Inferred from physical interaction PubMed 12815063. Source: MGI adult locomotory behavior Inferred from electronic annotation. Source: Compara cell-cell signaling Traceable author statement Ref.2Ref.1. Source: ProtInc heart development Traceable author statement Ref.2. Source: ProtInc negative regulation of cation channel activity Inferred from electronic annotation. Source: Compara nervous system development Traceable author statement Ref.1. Source: ProtInc neuromuscular process Inferred from electronic annotation. Source: Compara positive regulation of sodium ion transport Inferred from electronic annotation. Source: Compara signal transduction Traceable author statement Ref.2Ref.1. Source: ProtInc synaptic transmission Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular space Traceable author statement Ref.2. Source: ProtInc nucleus Inferred from direct assay Ref.1. Source: MGI
Molecular_function	growth factor activity Traceable author statement Ref.2Ref.1. Source: ProtInc heparin binding Inferred from direct assay PubMed 12815063. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P61328-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P61328-2)

Also known as: FGF-12B;

The sequence of this isoform differs from the canonical sequence as follows:
     1-66: MAAAIASSLIRQKRQARESNSDRVSASKRRSSPSKDGRSLCERHVLGVFSKVRFCSGRKRPVRRRP → MESK

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 243

243

Fibroblast growth factor 12

PRO_0000147604

Regions

Motif

11 – 38

28

Bipartite nuclear localization signal Potential

Natural variations

Alternative sequence

1 – 66

66

MAAAI…VRRRP → MESK in isoform 2.

VSP_010222

Experimental info

Sequence conflict

190

1

K → E in AAB18786. Ref.2

Sequence conflict

211

1

P → Q in AAH22524. Ref.5

Sequence conflict

229 – 243

15

TPTMN…NQDST → HHHDGGKL in AAB18786. Ref.2

Secondary structure

1

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243

Helix Strand Turn

Details...

Beta strand

73 – 79

7

Turn

80 – 82

3

Beta strand

83 – 87

5

Beta strand

93 – 97

5

Helix

102 – 104

3

Beta strand

106 – 112

7

Beta strand

115 – 120

6

Turn

121 – 123

3

Beta strand

126 – 129

4

Beta strand

135 – 140

6

Helix

143 – 145

3

Beta strand

147 – 152

6

Turn

153 – 155

3

Beta strand

156 – 165

10

Turn

167 – 169

3

Beta strand

172 – 174

3

Beta strand

181 – 183

3

Helix

186 – 188

3

Helix

194 – 196

3

Beta strand

198 – 202

5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 10, 2004. Version 1. Checksum: 773ED10B5BDD033C	FASTA	243	27,399
	10 20 30 40 50 60 MAAAIASSLI RQKRQARESN SDRVSASKRR SSPSKDGRSL CERHVLGVFS KVRFCSGRKR 70 80 90 100 110 120 PVRRRPEPQL KGIVTRLFSQ QGYFLQMHPD GTIDGTKDEN SDYTLFNLIP VGLRVVAIQG 130 140 150 160 170 180 VKASLYVAMN GEGYLYSSDV FTPECKFKES VFENYYVIYS STLYRQQESG RAWFLGLNKE 190 200 210 220 230 240 GQIMKGNRVK KTKPSSHFVP KPIEVCMYRE PSLHEIGEKQ GRSRKSSGTP TMNGGKVVNQ DST
	Isoform 2 (FGF-12B) [UniParc]. Checksum: E150D8758875C8B7	FASTA	181	20,424
	10 20 30 40 50 60 MESKEPQLKG IVTRLFSQQG YFLQMHPDGT IDGTKDENSD YTLFNLIPVG LRVVAIQGVK 70 80 90 100 110 120 ASLYVAMNGE GYLYSSDVFT PECKFKESVF ENYYVIYSST LYRQQESGRA WFLGLNKEGQ 130 140 150 160 170 180 IMKGNRVKKT KPSSHFVPKP IEVCMYREPS LHEIGEKQGR SRKSSGTPTM NGGKVVNQDS T

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Fibroblast growth factor (FGF) homologous factors: new members of the FGF family implicated in nervous system development." Smallwood P.M., Munoz-Sanjuan I., Tong P., Macke J.P., Hendry S.H., Gilbert D.J., Copeland N.G., Jenkins N.A., Nathans J. Proc. Natl. Acad. Sci. U.S.A. 93:9850-9857(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Retina.
[2]	"Cloning and characterization of a cDNA encoding a novel fibroblast growth factor preferentially expressed in human heart." Kok L.D.S., Tsui S.K.W., Waye M.M.Y., Liew C.C., Lee C.-Y., Fung K.-P. Biochem. Biophys. Res. Commun. 255:717-721(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Heart.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Hippocampus and Thalamus.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U66197 mRNA. Translation: AAB18913.1.
U76381 mRNA. Translation: AAB18786.3.
AK125307 mRNA. Translation: BAG54181.1.
AK313671 mRNA. Translation: BAG36423.1.
AK312513 mRNA. Translation: BAG35414.1.
CH471052 Genomic DNA. Translation: EAW78084.1.
BC022524 mRNA. Translation: AAH22524.1.

IPI

IPI00024249.
IPI00410208.

PIR

JG0184.

RefSeq

NP_004104.3. NM_004113.5.
NP_066360.1. NM_021032.4.

UniGene

Hs.390250.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1Q1U	X-ray	1.70	A	67-205	[»]

ProteinModelPortal

P61328.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-59850N.

STRING

9606.ENSP00000413496.

PTM databases

PhosphoSite

P61328.

Polymorphism databases

DMDM

47117683.

Proteomic databases

PaxDb

P61328.

PRIDE

P61328.

Protocols and materials databases

DNASU

2257.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000264730; ENSP00000264730; ENSG00000114279.
ENST00000445105; ENSP00000393686; ENSG00000114279.
ENST00000450716; ENSP00000397635; ENSG00000114279.
ENST00000454309; ENSP00000413496; ENSG00000114279.

GeneID

2257.

KEGG

hsa:2257.

UCSC

uc003fsx.3. human.
uc003fsy.3. human.

Organism-specific databases

CTD

2257.

GeneCards

GC03M191859.

HGNC

HGNC:3668. FGF12.

MIM

601513. gene.

neXtProt

NX_P61328.

PharmGKB

PA28108.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG275698.

HOVERGEN

HBG007580.

InParanoid

P61328.

KO

K04358.

OMA

LYVAMNA.

OrthoDB

EOG4G7C03.

PhylomeDB

P61328.

Gene expression databases

ArrayExpress

P61328.

Bgee

P61328.

CleanEx

HS_FGF12.

Genevestigator

P61328.

GermOnline

ENSG00000114279. Homo sapiens.

Family and domain databases

InterPro

IPR008996. Cytokine_IL1-like.
IPR002209. GF_heparin-bd.
IPR002348. IL1_HBGF.
[Graphical view]

PANTHER

PTHR11486. PTHR11486. 1 hit.

Pfam

PF00167. FGF. 1 hit.
[Graphical view]

PRINTS

PR00263. HBGFFGF.
PR00262. IL1HBGF.

SMART

SM00442. FGF. 1 hit.
[Graphical view]

SUPFAM

SSF50353. Cytok_IL1_like. 1 hit.

PROSITE

PS00247. HBGF_FGF. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

FGF12. human.

EvolutionaryTrace

P61328.

GenomeRNAi

2257.

NextBio

9143.

SOURCE

Search...

Entry information

Entry name

FGF12_HUMAN

Accession

Primary (citable) accession number: P61328
Secondary accession number(s): B2R6B7 , B2R976, O35339, P70376, Q8TBG5, Q92912, Q93001

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	May 10, 2004
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.