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P61327

- MGN_MOUSE

UniProt

P61327 - MGN_MOUSE

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Protein
Protein mago nashi homolog
Gene
Magoh
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator WIBG/PYM leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms; the function is different from the established EJC assembly By similarity.

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. RNA splicing Source: UniProtKB-KW
  2. female gamete generation Source: UniProtKB
  3. mRNA processing Source: UniProtKB-KW
  4. mRNA transport Source: UniProtKB-KW
  5. nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: UniProtKB-KW
  6. regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
  7. regulation of translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing, mRNA transport, Nonsense-mediated mRNA decay, Translation regulation, Transport

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiREACT_198528. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
Protein mago nashi homolog
Gene namesi
Name:Magoh
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:1330312. Magoh.

Subcellular locationi

Nucleus By similarity. Nucleus speckle By similarity. Cytoplasm By similarity
Note: Detected in granule-like structures in the dendroplasm. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Colocalizes with the core EJC, ALYREF/THOC4, NXF1 and UAP56 in the nucleus and nuclear speckles By similarity.

GO - Cellular componenti

  1. catalytic step 2 spliceosome Source: Ensembl
  2. cytoplasm Source: UniProtKB-SubCell
  3. exon-exon junction complex Source: Ensembl
  4. nuclear speck Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 146146Protein mago nashi homolog
PRO_0000174147Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP61327.
PaxDbiP61327.
PRIDEiP61327.

PTM databases

PhosphoSiteiP61327.

Expressioni

Gene expression databases

ArrayExpressiP61327.
BgeeiP61327.
GenevestigatoriP61327.

Interactioni

Subunit structurei

Heterodimer with RBM8A. Part of the mRNA splicing-dependent exon junction complex (EJC) complex; the core complex contains CASC3, EIF4A3, MAGOH and RBM8A. Interacts with WIBG/PYM; the interaction is direct and dissociates the EJC from spliced mRNAs. Identified in the spliceosome C complex By similarity.

Protein-protein interaction databases

BioGridi201297. 1 interaction.
IntActiP61327. 1 interaction.
MINTiMINT-4101859.

Structurei

3D structure databases

ProteinModelPortaliP61327.
SMRiP61327. Positions 2-145.

Family & Domainsi

Sequence similaritiesi

Belongs to the mago nashi family.

Phylogenomic databases

eggNOGiNOG255527.
GeneTreeiENSGT00390000003156.
HOGENOMiHOG000207428.
HOVERGENiHBG004353.
InParanoidiA2A8E0.
KOiK12877.
OMAiKEDDTNW.
OrthoDBiEOG7F24VD.
PhylomeDBiP61327.
TreeFamiTF300128.

Family and domain databases

Gene3Di3.30.1560.10. 1 hit.
InterProiIPR004023. Mago_nashi.
[Graphical view]
PANTHERiPTHR12638. PTHR12638. 1 hit.
PfamiPF02792. Mago_nashi. 1 hit.
[Graphical view]
SUPFAMiSSF89817. SSF89817. 1 hit.

Sequencei

Sequence statusi: Complete.

P61327-1 [UniParc]FASTAAdd to Basket

« Hide

MESDFYLRYY VGHKGKFGHE FLEFEFRPDG KLRYANNSNY KNDVMIRKEA    50
YVHKSVMEEL KRIIDDSEIT KEDDALWPPP DRVGRQELEI VIGDEHISFT 100
TSKIGSLIDV NQSKDPEGLR VFYYLVQDLK CLVFSLIGLH FKIKPI 146
Length:146
Mass (Da):17,164
Last modified:May 10, 2004 - v1
Checksum:iFFAD0B075E045875
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF007862 mRNA. Translation: AAB66722.1.
AF035939 mRNA. Translation: AAC40044.1.
AK011512 mRNA. Translation: BAB27668.1.
AL611936 Genomic DNA. Translation: CAM23583.1.
CH466527 Genomic DNA. Translation: EDL30768.1.
BC018176 mRNA. Translation: AAH18176.1.
CCDSiCCDS18441.1.
RefSeqiNP_001269666.1. NM_001282737.1.
UniGeneiMm.808.

Genome annotation databases

EnsembliENSMUST00000030348; ENSMUSP00000030348; ENSMUSG00000028609.
GeneIDi17149.
KEGGimmu:17149.
UCSCiuc008uaj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF007862 mRNA. Translation: AAB66722.1 .
AF035939 mRNA. Translation: AAC40044.1 .
AK011512 mRNA. Translation: BAB27668.1 .
AL611936 Genomic DNA. Translation: CAM23583.1 .
CH466527 Genomic DNA. Translation: EDL30768.1 .
BC018176 mRNA. Translation: AAH18176.1 .
CCDSi CCDS18441.1.
RefSeqi NP_001269666.1. NM_001282737.1.
UniGenei Mm.808.

3D structure databases

ProteinModelPortali P61327.
SMRi P61327. Positions 2-145.
ModBasei Search...

Protein-protein interaction databases

BioGridi 201297. 1 interaction.
IntActi P61327. 1 interaction.
MINTi MINT-4101859.

PTM databases

PhosphoSitei P61327.

Proteomic databases

MaxQBi P61327.
PaxDbi P61327.
PRIDEi P61327.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000030348 ; ENSMUSP00000030348 ; ENSMUSG00000028609 .
GeneIDi 17149.
KEGGi mmu:17149.
UCSCi uc008uaj.1. mouse.

Organism-specific databases

CTDi 4116.
MGIi MGI:1330312. Magoh.

Phylogenomic databases

eggNOGi NOG255527.
GeneTreei ENSGT00390000003156.
HOGENOMi HOG000207428.
HOVERGENi HBG004353.
InParanoidi A2A8E0.
KOi K12877.
OMAi KEDDTNW.
OrthoDBi EOG7F24VD.
PhylomeDBi P61327.
TreeFami TF300128.

Enzyme and pathway databases

Reactomei REACT_198528. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

NextBioi 16162.
PROi P61327.
SOURCEi Search...

Gene expression databases

ArrayExpressi P61327.
Bgeei P61327.
Genevestigatori P61327.

Family and domain databases

Gene3Di 3.30.1560.10. 1 hit.
InterProi IPR004023. Mago_nashi.
[Graphical view ]
PANTHERi PTHR12638. PTHR12638. 1 hit.
Pfami PF02792. Mago_nashi. 1 hit.
[Graphical view ]
SUPFAMi SSF89817. SSF89817. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mago nashi mediates the posterior follicle cell-to-oocyte signal to organize axis formation in Drosophila."
    Newmark P.A., Mohr S.E., Gong L., Boswell R.E.
    Development 124:3197-3207(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The mammalian homologue of mago nashi encodes a serum-inducible protein."
    Zhao X.F., Colaizzo-Anas T., Nowak N.J., Shows T.B., Elliott R.W., Aplan P.D.
    Genomics 47:319-322(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiMGN_MOUSE
AccessioniPrimary (citable) accession number: P61327
Secondary accession number(s): A2A8E0, O35169, P50606
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: September 3, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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