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P61327

- MGN_MOUSE

UniProt

P61327 - MGN_MOUSE

Protein

Protein mago nashi homolog

Gene

Magoh

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
  1. Functioni

    Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator WIBG/PYM leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms; the function is different from the established EJC assembly By similarity.By similarity

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. female gamete generation Source: UniProtKB
    2. mRNA processing Source: UniProtKB-KW
    3. mRNA transport Source: UniProtKB-KW
    4. nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: UniProtKB-KW
    5. regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
    6. regulation of translation Source: UniProtKB-KW
    7. RNA splicing Source: UniProtKB-KW

    Keywords - Biological processi

    mRNA processing, mRNA splicing, mRNA transport, Nonsense-mediated mRNA decay, Translation regulation, Transport

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_198528. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein mago nashi homolog
    Gene namesi
    Name:Magoh
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:1330312. Magoh.

    Subcellular locationi

    Nucleus By similarity. Nucleus speckle By similarity. Cytoplasm By similarity
    Note: Detected in granule-like structures in the dendroplasm. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Colocalizes with the core EJC, ALYREF/THOC4, NXF1 and UAP56 in the nucleus and nuclear speckles By similarity.By similarity

    GO - Cellular componenti

    1. catalytic step 2 spliceosome Source: Ensembl
    2. cytoplasm Source: UniProtKB-SubCell
    3. exon-exon junction complex Source: Ensembl
    4. nuclear speck Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Spliceosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 146146Protein mago nashi homologPRO_0000174147Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP61327.
    PaxDbiP61327.
    PRIDEiP61327.

    PTM databases

    PhosphoSiteiP61327.

    Expressioni

    Gene expression databases

    ArrayExpressiP61327.
    BgeeiP61327.
    GenevestigatoriP61327.

    Interactioni

    Subunit structurei

    Heterodimer with RBM8A. Part of the mRNA splicing-dependent exon junction complex (EJC) complex; the core complex contains CASC3, EIF4A3, MAGOH and RBM8A. Interacts with WIBG/PYM; the interaction is direct and dissociates the EJC from spliced mRNAs. Identified in the spliceosome C complex By similarity.By similarity

    Protein-protein interaction databases

    BioGridi201297. 1 interaction.
    IntActiP61327. 1 interaction.
    MINTiMINT-4101859.

    Structurei

    3D structure databases

    ProteinModelPortaliP61327.
    SMRiP61327. Positions 2-145.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the mago nashi family.Curated

    Phylogenomic databases

    eggNOGiNOG255527.
    GeneTreeiENSGT00390000003156.
    HOGENOMiHOG000207428.
    HOVERGENiHBG004353.
    InParanoidiA2A8E0.
    KOiK12877.
    OMAiKEDDTNW.
    OrthoDBiEOG7F24VD.
    PhylomeDBiP61327.
    TreeFamiTF300128.

    Family and domain databases

    Gene3Di3.30.1560.10. 1 hit.
    InterProiIPR004023. Mago_nashi.
    [Graphical view]
    PANTHERiPTHR12638. PTHR12638. 1 hit.
    PfamiPF02792. Mago_nashi. 1 hit.
    [Graphical view]
    SUPFAMiSSF89817. SSF89817. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P61327-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESDFYLRYY VGHKGKFGHE FLEFEFRPDG KLRYANNSNY KNDVMIRKEA    50
    YVHKSVMEEL KRIIDDSEIT KEDDALWPPP DRVGRQELEI VIGDEHISFT 100
    TSKIGSLIDV NQSKDPEGLR VFYYLVQDLK CLVFSLIGLH FKIKPI 146
    Length:146
    Mass (Da):17,164
    Last modified:May 10, 2004 - v1
    Checksum:iFFAD0B075E045875
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF007862 mRNA. Translation: AAB66722.1.
    AF035939 mRNA. Translation: AAC40044.1.
    AK011512 mRNA. Translation: BAB27668.1.
    AL611936 Genomic DNA. Translation: CAM23583.1.
    CH466527 Genomic DNA. Translation: EDL30768.1.
    BC018176 mRNA. Translation: AAH18176.1.
    CCDSiCCDS18441.1.
    RefSeqiNP_001269666.1. NM_001282737.1.
    UniGeneiMm.808.

    Genome annotation databases

    EnsembliENSMUST00000030348; ENSMUSP00000030348; ENSMUSG00000028609.
    GeneIDi17149.
    KEGGimmu:17149.
    UCSCiuc008uaj.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF007862 mRNA. Translation: AAB66722.1 .
    AF035939 mRNA. Translation: AAC40044.1 .
    AK011512 mRNA. Translation: BAB27668.1 .
    AL611936 Genomic DNA. Translation: CAM23583.1 .
    CH466527 Genomic DNA. Translation: EDL30768.1 .
    BC018176 mRNA. Translation: AAH18176.1 .
    CCDSi CCDS18441.1.
    RefSeqi NP_001269666.1. NM_001282737.1.
    UniGenei Mm.808.

    3D structure databases

    ProteinModelPortali P61327.
    SMRi P61327. Positions 2-145.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201297. 1 interaction.
    IntActi P61327. 1 interaction.
    MINTi MINT-4101859.

    PTM databases

    PhosphoSitei P61327.

    Proteomic databases

    MaxQBi P61327.
    PaxDbi P61327.
    PRIDEi P61327.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000030348 ; ENSMUSP00000030348 ; ENSMUSG00000028609 .
    GeneIDi 17149.
    KEGGi mmu:17149.
    UCSCi uc008uaj.1. mouse.

    Organism-specific databases

    CTDi 4116.
    MGIi MGI:1330312. Magoh.

    Phylogenomic databases

    eggNOGi NOG255527.
    GeneTreei ENSGT00390000003156.
    HOGENOMi HOG000207428.
    HOVERGENi HBG004353.
    InParanoidi A2A8E0.
    KOi K12877.
    OMAi KEDDTNW.
    OrthoDBi EOG7F24VD.
    PhylomeDBi P61327.
    TreeFami TF300128.

    Enzyme and pathway databases

    Reactomei REACT_198528. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

    Miscellaneous databases

    NextBioi 16162.
    PROi P61327.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P61327.
    Bgeei P61327.
    Genevestigatori P61327.

    Family and domain databases

    Gene3Di 3.30.1560.10. 1 hit.
    InterProi IPR004023. Mago_nashi.
    [Graphical view ]
    PANTHERi PTHR12638. PTHR12638. 1 hit.
    Pfami PF02792. Mago_nashi. 1 hit.
    [Graphical view ]
    SUPFAMi SSF89817. SSF89817. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Mago nashi mediates the posterior follicle cell-to-oocyte signal to organize axis formation in Drosophila."
      Newmark P.A., Mohr S.E., Gong L., Boswell R.E.
      Development 124:3197-3207(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The mammalian homologue of mago nashi encodes a serum-inducible protein."
      Zhao X.F., Colaizzo-Anas T., Nowak N.J., Shows T.B., Elliott R.W., Aplan P.D.
      Genomics 47:319-322(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiMGN_MOUSE
    AccessioniPrimary (citable) accession number: P61327
    Secondary accession number(s): A2A8E0, O35169, P50606
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2004
    Last sequence update: May 10, 2004
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3