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Protein

Protein mago nashi homolog

Gene

Magoh

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms; the function is different from the established EJC assembly (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing, mRNA splicing, mRNA transport, Nonsense-mediated mRNA decay, Translation regulation, Transport

Enzyme and pathway databases

ReactomeiR-MMU-109688 Cleavage of Growing Transcript in the Termination Region
R-MMU-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript
R-MMU-72163 mRNA Splicing - Major Pathway
R-MMU-72187 mRNA 3'-end processing
R-MMU-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
Protein mago nashi homolog
Gene namesi
Name:Magoh
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1330312 Magoh

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001741471 – 146Protein mago nashi homologAdd BLAST146

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP61327
PaxDbiP61327
PeptideAtlasiP61327
PRIDEiP61327
TopDownProteomicsiP61327

PTM databases

iPTMnetiP61327
PhosphoSitePlusiP61327

Expressioni

Gene expression databases

BgeeiENSMUSG00000028609
GenevisibleiP61327 MM

Interactioni

Subunit structurei

Heterodimer with RBM8A. Part of the mRNA splicing-dependent exon junction complex (EJC) complex; the core complex contains CASC3, EIF4A3, MAGOH and RBM8A. Interacts with PYM1; the interaction is direct and dissociates the EJC from spliced mRNAs. Identified in the spliceosome C complex (By similarity).By similarity

Protein-protein interaction databases

BioGridi201297, 26 interactors
IntActiP61327, 27 interactors
MINTiP61327
STRINGi10090.ENSMUSP00000030348

Structurei

3D structure databases

ProteinModelPortaliP61327
SMRiP61327
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the mago nashi family.Curated

Phylogenomic databases

eggNOGiKOG3392 Eukaryota
ENOG4111FJ7 LUCA
GeneTreeiENSGT00390000003156
HOGENOMiHOG000207428
HOVERGENiHBG004353
InParanoidiP61327
KOiK12877
OMAiEFDFRVV
OrthoDBiEOG091G0UKA
PhylomeDBiP61327
TreeFamiTF300128

Family and domain databases

CDDicd11295 Mago_nashi, 1 hit
Gene3Di3.30.1560.10, 1 hit
InterProiView protein in InterPro
IPR004023 Mago_nashi
IPR036605 Mago_nashi_sf
PANTHERiPTHR12638 PTHR12638, 1 hit
PfamiView protein in Pfam
PF02792 Mago_nashi, 1 hit
SUPFAMiSSF89817 SSF89817, 1 hit

Sequencei

Sequence statusi: Complete.

P61327-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESDFYLRYY VGHKGKFGHE FLEFEFRPDG KLRYANNSNY KNDVMIRKEA
60 70 80 90 100
YVHKSVMEEL KRIIDDSEIT KEDDALWPPP DRVGRQELEI VIGDEHISFT
110 120 130 140
TSKIGSLIDV NQSKDPEGLR VFYYLVQDLK CLVFSLIGLH FKIKPI
Length:146
Mass (Da):17,164
Last modified:May 10, 2004 - v1
Checksum:iFFAD0B075E045875
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF007862 mRNA Translation: AAB66722.1
AF035939 mRNA Translation: AAC40044.1
AK011512 mRNA Translation: BAB27668.1
AL611936 Genomic DNA Translation: CAM23583.1
CH466527 Genomic DNA Translation: EDL30768.1
BC018176 mRNA Translation: AAH18176.1
CCDSiCCDS18441.1
RefSeqiNP_001269666.1, NM_001282737.1
UniGeneiMm.808

Genome annotation databases

EnsembliENSMUST00000030348; ENSMUSP00000030348; ENSMUSG00000028609
GeneIDi17149
KEGGimmu:17149
UCSCiuc008uaj.2 mouse

Similar proteinsi

Entry informationi

Entry nameiMGN_MOUSE
AccessioniPrimary (citable) accession number: P61327
Secondary accession number(s): A2A8E0, O35169, P50606
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: March 28, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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