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Protein

Protein mago nashi homolog

Gene

MAGOH

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly.3 Publications

GO - Molecular functioni

  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

  • mRNA 3'-end processing Source: Reactome
  • mRNA export from nucleus Source: Reactome
  • mRNA splicing, via spliceosome Source: UniProtKB
  • nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: UniProtKB
  • regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
  • regulation of translation Source: UniProtKB-KW
  • RNA export from nucleus Source: Reactome
  • termination of RNA polymerase II transcription Source: Reactome
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing, mRNA transport, Nonsense-mediated mRNA decay, Translation regulation, Transport

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000162385-MONOMER.
ReactomeiR-HSA-109688. Cleavage of Growing Transcript in the Termination Region.
R-HSA-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72187. mRNA 3'-end processing.
R-HSA-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Protein family/group databases

TCDBi3.A.18.1.1. the nuclear mrna exporter (mrna-e) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein mago nashi homolog
Gene namesi
Name:MAGOH
Synonyms:MAGOHA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:6815. MAGOH.

Subcellular locationi

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: UniProtKB
  • cytosol Source: Reactome
  • exon-exon junction complex Source: UniProtKB
  • nuclear speck Source: UniProtKB-SubCell
  • nucleoplasm Source: Reactome
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi16 – 17KF → EA: Impaired nonsense-mediated decay activity. 1 Publication2
Mutagenesisi41 – 42KN → DA: Complete loss of nonsense-mediated decay activity. 1 Publication2
Mutagenesisi66 – 68DSE → RSR: Slightly reduced nonsense-mediated decay activity. 1 Publication3
Mutagenesisi68E → R: Abolishes interaction with PYM1 leading to increase EJC association with splices mRNAs; when associated with R-72; K-73 and R-117. 1 Publication1
Mutagenesisi72 – 73ED → RK: Fully active. 1 Publication2
Mutagenesisi72E → R: Abolishes interaction with PYM1 leading to increase EJC association with splices mRNAs; when associated with R-68; K-73 and R-117. 1 Publication1
Mutagenesisi73D → K: Abolishes interaction with PYM1 leading to increase EJC association with splices mRNAs; when associated with R-68; R-72 and R-117. 1 Publication1
Mutagenesisi85 – 87RQE → EQR: Fully active. 1 Publication3
Mutagenesisi117E → R: Abolishes interaction with PYM1 leading to increase EJC association with splices mRNAs; when associated with R-68; R-72 and K-73. 1 Publication1
Mutagenesisi130 – 134KCLVF → ECLVA: Complete loss of nonsense-mediated decay activity. 1 Publication5
Mutagenesisi136L → R: Complete loss of nonsense-mediated decay activity. 1 Publication1

Organism-specific databases

DisGeNETi4116.
OpenTargetsiENSG00000162385.
PharmGKBiPA30563.

Polymorphism and mutation databases

BioMutaiMAGOH.
DMDMi47117708.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001741451 – 146Protein mago nashi homologAdd BLAST146

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP61326.
MaxQBiP61326.
PaxDbiP61326.
PeptideAtlasiP61326.
PRIDEiP61326.
TopDownProteomicsiP61326-1. [P61326-1]

PTM databases

iPTMnetiP61326.
PhosphoSitePlusiP61326.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSG00000162385.
CleanExiHS_MAGOH.
GenevisibleiP61326. HS.

Organism-specific databases

HPAiCAB015425.
HPA043036.
HPA047754.

Interactioni

Subunit structurei

Heterodimer with RBM8A. Part of the mRNA splicing-dependent exon junction complex (EJC) complex; the core complex contains CASC3, EIF4A3, MAGOH and RBM8A. Interacts with PYM1; the interaction is direct and dissociates the EJC from spliced mRNAs. Identified in the spliceosome C complex.9 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CASC3O152345EBI-299134,EBI-299118
EIF4A3P3891920EBI-299134,EBI-299104
GOLGA2Q083793EBI-299134,EBI-618309
IPO13O948294EBI-299134,EBI-747310
PYM1Q9BRP812EBI-299134,EBI-2352802
RBM8AQ9Y5S930EBI-299134,EBI-447231
TADA2AO754783EBI-299134,EBI-742268

Protein-protein interaction databases

BioGridi110290. 188 interactors.
DIPiDIP-33069N.
IntActiP61326. 137 interactors.
MINTiMINT-1574804.
STRINGi9606.ENSP00000360525.

Structurei

Secondary structure

1146
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 15Combined sources11
Beta strandi18 – 26Combined sources9
Beta strandi30 – 38Combined sources9
Turni40 – 42Combined sources3
Beta strandi44 – 52Combined sources9
Helixi54 – 67Combined sources14
Helixi69 – 71Combined sources3
Beta strandi74 – 77Combined sources4
Beta strandi85 – 92Combined sources8
Beta strandi95 – 101Combined sources7
Helixi107 – 110Combined sources4
Beta strandi113 – 115Combined sources3
Helixi116 – 141Combined sources26

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1P27X-ray2.00A/C2-145[»]
2HYIX-ray2.30A/G1-146[»]
2J0QX-ray3.20C/F1-146[»]
2J0SX-ray2.21C1-146[»]
2XB2X-ray3.40C/Y1-146[»]
3EX7X-ray2.30A/E1-146[»]
ProteinModelPortaliP61326.
SMRiP61326.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61326.

Family & Domainsi

Sequence similaritiesi

Belongs to the mago nashi family.Curated

Phylogenomic databases

eggNOGiKOG3392. Eukaryota.
ENOG4111FJ7. LUCA.
GeneTreeiENSGT00390000003156.
HOGENOMiHOG000207428.
HOVERGENiHBG004353.
InParanoidiP61326.
KOiK12877.
OMAiQDSEIMK.
OrthoDBiEOG091G0UKA.
PhylomeDBiP61326.
TreeFamiTF300128.

Family and domain databases

CDDicd11295. Mago_nashi. 1 hit.
Gene3Di3.30.1560.10. 1 hit.
InterProiIPR004023. Mago_nashi.
[Graphical view]
PANTHERiPTHR12638. PTHR12638. 1 hit.
PfamiPF02792. Mago_nashi. 1 hit.
[Graphical view]
SUPFAMiSSF89817. SSF89817. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P61326-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESDFYLRYY VGHKGKFGHE FLEFEFRPDG KLRYANNSNY KNDVMIRKEA
60 70 80 90 100
YVHKSVMEEL KRIIDDSEIT KEDDALWPPP DRVGRQELEI VIGDEHISFT
110 120 130 140
TSKIGSLIDV NQSKDPEGLR VFYYLVQDLK CLVFSLIGLH FKIKPI
Length:146
Mass (Da):17,164
Last modified:May 10, 2004 - v1
Checksum:iFFAD0B075E045875
GO
Isoform 2 (identifier: P61326-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     50-86: Missing.

Note: No experimental confirmation available.
Show »
Length:109
Mass (Da):12,844
Checksum:iD232443C8370F0CF
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05624650 – 86Missing in isoform 2. 1 PublicationAdd BLAST37

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF035940 mRNA. Translation: AAC39606.1.
AF067173 mRNA. Translation: AAD32457.1.
AK297895 mRNA. Translation: BAG60216.1.
AK312113 mRNA. Translation: BAG35049.1.
AL606760 Genomic DNA. Translation: CAI18914.1.
CH471059 Genomic DNA. Translation: EAX06747.1.
CH471059 Genomic DNA. Translation: EAX06748.1.
BC018211 mRNA. Translation: AAH18211.1.
CCDSiCCDS577.1. [P61326-1]
RefSeqiNP_002361.1. NM_002370.3. [P61326-1]
UniGeneiHs.421576.

Genome annotation databases

EnsembliENST00000371466; ENSP00000360521; ENSG00000162385. [P61326-2]
ENST00000371470; ENSP00000360525; ENSG00000162385. [P61326-1]
GeneIDi4116.
KEGGihsa:4116.
UCSCiuc001cvf.2. human. [P61326-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF035940 mRNA. Translation: AAC39606.1.
AF067173 mRNA. Translation: AAD32457.1.
AK297895 mRNA. Translation: BAG60216.1.
AK312113 mRNA. Translation: BAG35049.1.
AL606760 Genomic DNA. Translation: CAI18914.1.
CH471059 Genomic DNA. Translation: EAX06747.1.
CH471059 Genomic DNA. Translation: EAX06748.1.
BC018211 mRNA. Translation: AAH18211.1.
CCDSiCCDS577.1. [P61326-1]
RefSeqiNP_002361.1. NM_002370.3. [P61326-1]
UniGeneiHs.421576.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1P27X-ray2.00A/C2-145[»]
2HYIX-ray2.30A/G1-146[»]
2J0QX-ray3.20C/F1-146[»]
2J0SX-ray2.21C1-146[»]
2XB2X-ray3.40C/Y1-146[»]
3EX7X-ray2.30A/E1-146[»]
ProteinModelPortaliP61326.
SMRiP61326.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110290. 188 interactors.
DIPiDIP-33069N.
IntActiP61326. 137 interactors.
MINTiMINT-1574804.
STRINGi9606.ENSP00000360525.

Protein family/group databases

TCDBi3.A.18.1.1. the nuclear mrna exporter (mrna-e) family.

PTM databases

iPTMnetiP61326.
PhosphoSitePlusiP61326.

Polymorphism and mutation databases

BioMutaiMAGOH.
DMDMi47117708.

Proteomic databases

EPDiP61326.
MaxQBiP61326.
PaxDbiP61326.
PeptideAtlasiP61326.
PRIDEiP61326.
TopDownProteomicsiP61326-1. [P61326-1]

Protocols and materials databases

DNASUi4116.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000371466; ENSP00000360521; ENSG00000162385. [P61326-2]
ENST00000371470; ENSP00000360525; ENSG00000162385. [P61326-1]
GeneIDi4116.
KEGGihsa:4116.
UCSCiuc001cvf.2. human. [P61326-1]

Organism-specific databases

CTDi4116.
DisGeNETi4116.
GeneCardsiMAGOH.
HGNCiHGNC:6815. MAGOH.
HPAiCAB015425.
HPA043036.
HPA047754.
MIMi602603. gene.
neXtProtiNX_P61326.
OpenTargetsiENSG00000162385.
PharmGKBiPA30563.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3392. Eukaryota.
ENOG4111FJ7. LUCA.
GeneTreeiENSGT00390000003156.
HOGENOMiHOG000207428.
HOVERGENiHBG004353.
InParanoidiP61326.
KOiK12877.
OMAiQDSEIMK.
OrthoDBiEOG091G0UKA.
PhylomeDBiP61326.
TreeFamiTF300128.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000162385-MONOMER.
ReactomeiR-HSA-109688. Cleavage of Growing Transcript in the Termination Region.
R-HSA-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72187. mRNA 3'-end processing.
R-HSA-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

ChiTaRSiMAGOH. human.
EvolutionaryTraceiP61326.
GeneWikiiMAGOH.
GenomeRNAii4116.
PROiP61326.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000162385.
CleanExiHS_MAGOH.
GenevisibleiP61326. HS.

Family and domain databases

CDDicd11295. Mago_nashi. 1 hit.
Gene3Di3.30.1560.10. 1 hit.
InterProiIPR004023. Mago_nashi.
[Graphical view]
PANTHERiPTHR12638. PTHR12638. 1 hit.
PfamiPF02792. Mago_nashi. 1 hit.
[Graphical view]
SUPFAMiSSF89817. SSF89817. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMGN_HUMAN
AccessioniPrimary (citable) accession number: P61326
Secondary accession number(s): B1ARP8
, B2R5A2, O35169, P50606, Q5SW69
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: November 30, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.