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P61320

- LOLB_ECOLI

UniProt

P61320 - LOLB_ECOLI

Protein

Outer-membrane lipoprotein LolB

Gene

lolB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (10 May 2004)
      Previous versions | rss
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    Functioni

    Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the LolA protein. Essential for E.coli viability.

    GO - Molecular functioni

    1. lipopeptide binding Source: EcoCyc
    2. protein transporter activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. lipid localization Source: EcoCyc
    2. lipoprotein metabolic process Source: EcoCyc
    3. localization within membrane Source: EcoCyc

    Keywords - Molecular functioni

    Chaperone

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11293-MONOMER.
    ECOL316407:JW1200-MONOMER.

    Protein family/group databases

    TCDBi1.B.46.1.1. the outer membrane lolab lipoprotein insertion apparatus (lolab) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Outer-membrane lipoprotein LolB
    Gene namesi
    Name:lolB
    Synonyms:hemM, ychC
    Ordered Locus Names:b1209, JW1200
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11293. lolB.

    Subcellular locationi

    GO - Cellular componenti

    1. cell outer membrane Source: EcoCyc

    Keywords - Cellular componenti

    Cell outer membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Add
    BLAST
    Chaini22 – 207186Outer-membrane lipoprotein LolBPRO_0000018296Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi22 – 221N-palmitoyl cysteine1 Publication
    Lipidationi22 – 221S-diacylglycerol cysteine1 Publication

    Keywords - PTMi

    Lipoprotein, Palmitate

    Proteomic databases

    PaxDbiP61320.
    PRIDEiP61320.

    Expressioni

    Gene expression databases

    GenevestigatoriP61320.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    DIPiDIP-35933N.
    IntActiP61320. 7 interactions.
    MINTiMINT-7259909.
    STRINGi511145.b1209.

    Structurei

    Secondary structure

    1
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi34 – 363
    Helixi37 – 4711
    Beta strandi51 – 6111
    Beta strandi66 – 7611
    Turni77 – 793
    Beta strandi80 – 867
    Beta strandi92 – 998
    Beta strandi102 – 1065
    Beta strandi112 – 1165
    Helixi118 – 1269
    Helixi132 – 1387
    Turni139 – 1413
    Beta strandi149 – 1513
    Beta strandi157 – 16610
    Beta strandi168 – 1736
    Beta strandi180 – 1823
    Beta strandi187 – 1915
    Beta strandi196 – 20712

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1IWMX-ray1.90A/B22-207[»]
    1IWNX-ray2.20A22-207[»]
    ProteinModelPortaliP61320.
    SMRiP61320. Positions 31-207.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP61320.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LolB family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3017.
    HOGENOMiHOG000119778.
    KOiK02494.
    OMAiYQTRGSF.
    OrthoDBiEOG6R5C6M.

    Family and domain databases

    HAMAPiMF_00233. LolB.
    InterProiIPR029046. LolA/LolB/LppX.
    IPR004565. OM_lipoprot_LolB.
    [Graphical view]
    PfamiPF03550. LolB. 1 hit.
    [Graphical view]
    SUPFAMiSSF89392. SSF89392. 1 hit.
    TIGRFAMsiTIGR00548. lolB. 1 hit.
    PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P61320-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPLPDFRLIR LLPLAALVLT ACSVTTPKGP GKSPDSPQWR QHQQDVRNLN    50
    QYQTRGAFAY ISDQQKVYAR FFWQQTGQDR YRLLLTNPLG STELELNAQP 100
    GNVQLVDNKG QRYTADDAEE MIGKLTGMPI PLNSLRQWIL GLPGDATDYK 150
    LDDQYRLSEI TYSQNGKNWK VVYGGYDTKT QPAMPANMEL TDGGQRIKLK 200
    MDNWIVK 207
    Length:207
    Mass (Da):23,551
    Last modified:May 10, 2004 - v1
    Checksum:iC517F6E7353B8551
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti12 – 121L → V in BAA01105. (PubMed:1427085)Curated
    Sequence conflicti41 – 422QH → HD in AAC43435. (PubMed:7543480)Curated
    Sequence conflicti176 – 20732YDTKT…NWIVK → MTPKRNLRCQPIWNSPTVVN ASS in AAC43435. (PubMed:7543480)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M77237 Genomic DNA. Translation: AAA24433.1.
    D10264 Genomic DNA. Translation: BAA01105.1.
    U18555 Genomic DNA. Translation: AAC43435.1.
    U00096 Genomic DNA. Translation: AAC74293.1.
    AP009048 Genomic DNA. Translation: BAA36067.1.
    PIRiA47706.
    RefSeqiNP_415727.1. NC_000913.3.
    YP_489476.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74293; AAC74293; b1209.
    BAA36067; BAA36067; BAA36067.
    GeneIDi12931820.
    945775.
    KEGGiecj:Y75_p1181.
    eco:b1209.
    PATRICi32117670. VBIEscCol129921_1257.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M77237 Genomic DNA. Translation: AAA24433.1 .
    D10264 Genomic DNA. Translation: BAA01105.1 .
    U18555 Genomic DNA. Translation: AAC43435.1 .
    U00096 Genomic DNA. Translation: AAC74293.1 .
    AP009048 Genomic DNA. Translation: BAA36067.1 .
    PIRi A47706.
    RefSeqi NP_415727.1. NC_000913.3.
    YP_489476.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1IWM X-ray 1.90 A/B 22-207 [» ]
    1IWN X-ray 2.20 A 22-207 [» ]
    ProteinModelPortali P61320.
    SMRi P61320. Positions 31-207.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-35933N.
    IntActi P61320. 7 interactions.
    MINTi MINT-7259909.
    STRINGi 511145.b1209.

    Protein family/group databases

    TCDBi 1.B.46.1.1. the outer membrane lolab lipoprotein insertion apparatus (lolab) family.

    Proteomic databases

    PaxDbi P61320.
    PRIDEi P61320.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74293 ; AAC74293 ; b1209 .
    BAA36067 ; BAA36067 ; BAA36067 .
    GeneIDi 12931820.
    945775.
    KEGGi ecj:Y75_p1181.
    eco:b1209.
    PATRICi 32117670. VBIEscCol129921_1257.

    Organism-specific databases

    EchoBASEi EB1270.
    EcoGenei EG11293. lolB.

    Phylogenomic databases

    eggNOGi COG3017.
    HOGENOMi HOG000119778.
    KOi K02494.
    OMAi YQTRGSF.
    OrthoDBi EOG6R5C6M.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG11293-MONOMER.
    ECOL316407:JW1200-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P61320.
    PROi P61320.

    Gene expression databases

    Genevestigatori P61320.

    Family and domain databases

    HAMAPi MF_00233. LolB.
    InterProi IPR029046. LolA/LolB/LppX.
    IPR004565. OM_lipoprot_LolB.
    [Graphical view ]
    Pfami PF03550. LolB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF89392. SSF89392. 1 hit.
    TIGRFAMsi TIGR00548. lolB. 1 hit.
    PROSITEi PS51257. PROKAR_LIPOPROTEIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of genes involved in the biosynthesis of delta-aminolevulinic acid in Escherichia coli."
      Ikemi M., Murakami K., Hashimoto M., Murooka Y.
      Gene 121:127-132(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Characterization of the hemA-prs region of the Escherichia coli and Salmonella typhimurium chromosomes: identification of two open reading frames and implications for prs expression."
      Post D.A., Hove-Jensen B., Switzer R.L.
      J. Gen. Microbiol. 139:259-266(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    3. "Expression of genes kdsA and kdsB involved in 3-deoxy-D-manno-octulosonic acid metabolism and biosynthesis of enterobacterial lipopolysaccharide is growth phase regulated primarily at the transcriptional level in Escherichia coli K-12."
      Strohmaier H., Remler P., Renner W., Hoegenauer G.
      J. Bacteriol. 177:4488-4500(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    6. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    7. "A novel outer membrane lipoprotein, LolB (HemM), involved in the LolA (p20)-dependent localization of lipoproteins to the outer membrane of Escherichia coli."
      Matsuyama S., Yokota N., Tokuda H.
      EMBO J. 16:6947-6955(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, DIACYLGLYCEROL AT CYS-22, PALMITOYLATION AT CYS-22, PARTIAL PROTEIN SEQUENCE.
    8. "Deletion of lolB, encoding an outer membrane lipoprotein, is lethal for Escherichia coli and causes accumulation of lipoprotein localization intermediates in the periplasm."
      Tanaka K., Matsuyama S., Tokuda H.
      J. Bacteriol. 183:6538-6542(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: IMPORTANCE FOR VIABILITY.
      Strain: K12.
    9. "Crystal structures of bacterial lipoprotein localization factors, LolA and LolB."
      Takeda K., Miyatake H., Yokota N., Matsuyama S., Tokuda H., Miki K.
      EMBO J. 22:3199-3209(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).

    Entry informationi

    Entry nameiLOLB_ECOLI
    AccessioniPrimary (citable) accession number: P61320
    Secondary accession number(s): P24208, Q46753
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2004
    Last sequence update: May 10, 2004
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    Was originally thought to be involved in delta-aminolevulinic acid biosynthesis.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3