P61320 (LOLB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Outer-membrane lipoprotein lolB

Gene names

Name:	lolB
Synonyms:	hemM, ychC
Ordered Locus Names:	b1209, JW1200

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the lolA protein. Essential for E.coli viability. HAMAP MF_00233
Subunit structure	Monomer.
Subcellular location	Cell outer membrane; Lipid-anchor HAMAP MF_00233.
Sequence similarities	Belongs to the lolB family.
Caution	Was originally (Ref.1) thought to be involved in delta-aminolevulinic acid biosynthesis.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cell membrane Cell outer membrane Membrane
Domain	Signal
Molecular function	Chaperone
PTM	Lipoprotein Palmitate
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell outer membrane Inferred from direct assay Ref.7. Source: EcoCyc plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	lipoprotein particle receptor activity Inferred from direct assay Ref.7. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 21

21

HAMAP MF_00233

Chain

22 – 207

186

Outer-membrane lipoprotein lolB HAMAP MF_00233

PRO_0000018296

Amino acid modifications

Lipidation

22

1

N-palmitoyl cysteine HAMAP MF_00233

Lipidation

22

1

S-diacylglycerol cysteine HAMAP MF_00233

Experimental info

Sequence conflict

12

1

L → V in BAA01105. Ref.1

Sequence conflict

41 – 42

2

QH → HD in AAC43435. Ref.3

Sequence conflict

176 – 207

32

YDTKT…NWIVK → MTPKRNLRCQPIWNSPTVVN ASS in AAC43435. Ref.3

Secondary structure

1

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207

Helix Strand Turn

Details...

Beta strand

34 – 36

3

Helix

37 – 47

11

Beta strand

51 – 61

11

Beta strand

66 – 76

11

Turn

77 – 79

3

Beta strand

80 – 86

7

Beta strand

92 – 99

8

Beta strand

102 – 106

5

Beta strand

112 – 116

5

Helix

118 – 126

9

Helix

132 – 138

7

Turn

139 – 141

3

Beta strand

149 – 151

3

Beta strand

157 – 166

10

Beta strand

168 – 173

6

Beta strand

180 – 182

3

Beta strand

187 – 191

5

Sequences

	Sequence		Length	Mass (Da)
	P61320 [UniParc]. Last modified May 10, 2004. Version 1. Checksum: C517F6E7353B8551	FASTA	207	23,551
	10 20 30 40 50 60 MPLPDFRLIR LLPLAALVLT ACSVTTPKGP GKSPDSPQWR QHQQDVRNLN QYQTRGAFAY 70 80 90 100 110 120 ISDQQKVYAR FFWQQTGQDR YRLLLTNPLG STELELNAQP GNVQLVDNKG QRYTADDAEE 130 140 150 160 170 180 MIGKLTGMPI PLNSLRQWIL GLPGDATDYK LDDQYRLSEI TYSQNGKNWK VVYGGYDTKT 190 200 QPAMPANMEL TDGGQRIKLK MDNWIVK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of genes involved in the biosynthesis of delta-aminolevulinic acid in Escherichia coli." Ikemi M., Murakami K., Hashimoto M., Murooka Y. Gene 121:127-132(1992) [PubMed: 1427085] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Characterization of the hemA-prs region of the Escherichia coli and Salmonella typhimurium chromosomes: identification of two open reading frames and implications for prs expression." Post D.A., Hove-Jensen B., Switzer R.L. J. Gen. Microbiol. 139:259-266(1993) [PubMed: 7679718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"Expression of genes kdsA and kdsB involved in 3-deoxy-D-manno-octulosonic acid metabolism and biosynthesis of enterobacterial lipopolysaccharide is growth phase regulated primarily at the transcriptional level in Escherichia coli K-12." Strohmaier H., Remler P., Renner W., Hoegenauer G. J. Bacteriol. 177:4488-4500(1995) [PubMed: 7543480] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[4]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[6]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]	"A novel outer membrane lipoprotein, LolB (HemM), involved in the LolA (p20)-dependent localization of lipoproteins to the outer membrane of Escherichia coli." Matsuyama S., Yokota N., Tokuda H. EMBO J. 16:6947-6955(1997) [PubMed: 9384574] [Abstract] Cited for: CHARACTERIZATION, PARTIAL PROTEIN SEQUENCE.
[8]	"Deletion of lolB, encoding an outer membrane lipoprotein, is lethal for Escherichia coli and causes accumulation of lipoprotein localization intermediates in the periplasm." Tanaka K., Matsuyama S., Tokuda H. J. Bacteriol. 183:6538-6542(2001) [PubMed: 11673422] [Abstract] Cited for: IMPORTANCE FOR VIABILITY. Strain: K12.
[9]	"Crystal structures of bacterial lipoprotein localization factors, LolA and LolB." Takeda K., Miyatake H., Yokota N., Matsuyama S., Tokuda H., Miki K. EMBO J. 22:3199-3209(2003) [PubMed: 12839983] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M77237 Genomic DNA. Translation: AAA24433.1.
D10264 Genomic DNA. Translation: BAA01105.1.
U18555 Genomic DNA. Translation: AAC43435.1.
U00096 Genomic DNA. Translation: AAC74293.1.
AP009048 Genomic DNA. Translation: BAA36067.1.

PIR

A47706.

RefSeq

NP_415727.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1IWM	X-ray	1.90	A/B	23-207	[»]
1IWN	X-ray	2.20	A	23-207	[»]

ProteinModelPortal

P61320.

SMR

P61320. Positions 31-207.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-35933N.

IntAct

P61320. 6 interactions.

MINT

MINT-7259909.

Protein family/group databases

TCDB

1.B.46.1.1. outer membrane LolAB lipoprotein insertion apparatus (LolAB) family.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000000481; EBESCP00000000481; EBESCG00000000401.
EBESCT00000015576; EBESCP00000014867; EBESCG00000014636.

GeneID

945775.

GenomeReviews

Gene locus JW1200 in contig AP009048_GR.
Gene locus b1209 in contig U00096_GR.

KEGG

ecj:JW1200.
eco:b1209.

PATRIC

32117670. VBIEscCol129921_1257.

Organism-specific databases

EchoBASE

EB1270.

EcoGene

EG11293. lolB.

Phylogenomic databases

eggNOG

COG3017.

GeneTree

EBGT00050000011802.

HOGENOM

HBG644390.

OMA

WLQRQDY.

ProtClustDB

PRK00022.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11293-MONOMER.

Gene expression databases

Genevestigator

P61320.

Family and domain databases

HAMAP

MF_00233. LolB.
[Tree]

InterPro

IPR004565. OM_lipoprot_LolB.
[Graphical view]

KO

K02494.

Pfam

PF03550. LolB. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00548. LolB. 1 hit.

PROSITE

PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

LOLB_ECOLI

Accession

Primary (citable) accession number: P61320
Secondary accession number(s): P24208, Q46753

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	May 10, 2004
Last modified:	January 25, 2012
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program