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Protein

Outer-membrane lipoprotein LolB

Gene

lolB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the LolA protein. Essential for E.coli viability.

GO - Molecular functioni

  • lipopeptide binding Source: EcoCyc
  • protein transporter activity Source: UniProtKB-HAMAP

GO - Biological processi

  • lipid localization Source: EcoCyc
  • lipoprotein localization to outer membrane Source: CACAO
  • lipoprotein metabolic process Source: EcoCyc
  • localization within membrane Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG11293-MONOMER.
ECOL316407:JW1200-MONOMER.

Protein family/group databases

TCDBi1.B.46.1.1. the outer membrane lolab lipoprotein insertion apparatus (lolab) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Outer-membrane lipoprotein LolB
Gene namesi
Name:lolB
Synonyms:hemM, ychC
Ordered Locus Names:b1209, JW1200
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11293. lolB.

Subcellular locationi

GO - Cellular componenti

  • cell outer membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Add BLAST21
ChainiPRO_000001829622 – 207Outer-membrane lipoprotein LolBAdd BLAST186

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi22N-palmitoyl cysteine1 Publication1
Lipidationi22S-diacylglycerol cysteine1 Publication1

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiP61320.
PRIDEiP61320.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4260814. 267 interactors.
DIPiDIP-35933N.
IntActiP61320. 7 interactors.
MINTiMINT-7259909.
STRINGi511145.b1209.

Structurei

Secondary structure

1207
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi34 – 36Combined sources3
Helixi37 – 47Combined sources11
Beta strandi51 – 61Combined sources11
Beta strandi66 – 76Combined sources11
Turni77 – 79Combined sources3
Beta strandi80 – 86Combined sources7
Beta strandi92 – 99Combined sources8
Beta strandi102 – 106Combined sources5
Beta strandi112 – 116Combined sources5
Helixi118 – 126Combined sources9
Helixi132 – 138Combined sources7
Turni139 – 141Combined sources3
Beta strandi149 – 151Combined sources3
Beta strandi157 – 166Combined sources10
Beta strandi168 – 173Combined sources6
Beta strandi180 – 182Combined sources3
Beta strandi187 – 191Combined sources5
Beta strandi196 – 207Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IWMX-ray1.90A/B22-207[»]
1IWNX-ray2.20A22-207[»]
ProteinModelPortaliP61320.
SMRiP61320.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61320.

Family & Domainsi

Sequence similaritiesi

Belongs to the LolB family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105C1U. Bacteria.
COG3017. LUCA.
HOGENOMiHOG000119778.
InParanoidiP61320.
KOiK02494.
OMAiYQTRGSF.

Family and domain databases

HAMAPiMF_00233. LolB. 1 hit.
InterProiIPR029046. LolA/LolB/LppX.
IPR004565. OM_lipoprot_LolB.
[Graphical view]
PfamiPF03550. LolB. 1 hit.
[Graphical view]
SUPFAMiSSF89392. SSF89392. 1 hit.
TIGRFAMsiTIGR00548. lolB. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61320-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLPDFRLIR LLPLAALVLT ACSVTTPKGP GKSPDSPQWR QHQQDVRNLN
60 70 80 90 100
QYQTRGAFAY ISDQQKVYAR FFWQQTGQDR YRLLLTNPLG STELELNAQP
110 120 130 140 150
GNVQLVDNKG QRYTADDAEE MIGKLTGMPI PLNSLRQWIL GLPGDATDYK
160 170 180 190 200
LDDQYRLSEI TYSQNGKNWK VVYGGYDTKT QPAMPANMEL TDGGQRIKLK

MDNWIVK
Length:207
Mass (Da):23,551
Last modified:May 10, 2004 - v1
Checksum:iC517F6E7353B8551
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12L → V in BAA01105 (PubMed:1427085).Curated1
Sequence conflicti41 – 42QH → HD in AAC43435 (PubMed:7543480).Curated2
Sequence conflicti176 – 207YDTKT…NWIVK → MTPKRNLRCQPIWNSPTVVN ASS in AAC43435 (PubMed:7543480).CuratedAdd BLAST32

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77237 Genomic DNA. Translation: AAA24433.1.
D10264 Genomic DNA. Translation: BAA01105.1.
U18555 Genomic DNA. Translation: AAC43435.1.
U00096 Genomic DNA. Translation: AAC74293.1.
AP009048 Genomic DNA. Translation: BAA36067.1.
PIRiA47706.
RefSeqiNP_415727.1. NC_000913.3.
WP_001130692.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74293; AAC74293; b1209.
BAA36067; BAA36067; BAA36067.
GeneIDi945775.
KEGGiecj:JW1200.
eco:b1209.
PATRICi32117670. VBIEscCol129921_1257.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77237 Genomic DNA. Translation: AAA24433.1.
D10264 Genomic DNA. Translation: BAA01105.1.
U18555 Genomic DNA. Translation: AAC43435.1.
U00096 Genomic DNA. Translation: AAC74293.1.
AP009048 Genomic DNA. Translation: BAA36067.1.
PIRiA47706.
RefSeqiNP_415727.1. NC_000913.3.
WP_001130692.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IWMX-ray1.90A/B22-207[»]
1IWNX-ray2.20A22-207[»]
ProteinModelPortaliP61320.
SMRiP61320.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260814. 267 interactors.
DIPiDIP-35933N.
IntActiP61320. 7 interactors.
MINTiMINT-7259909.
STRINGi511145.b1209.

Protein family/group databases

TCDBi1.B.46.1.1. the outer membrane lolab lipoprotein insertion apparatus (lolab) family.

Proteomic databases

PaxDbiP61320.
PRIDEiP61320.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74293; AAC74293; b1209.
BAA36067; BAA36067; BAA36067.
GeneIDi945775.
KEGGiecj:JW1200.
eco:b1209.
PATRICi32117670. VBIEscCol129921_1257.

Organism-specific databases

EchoBASEiEB1270.
EcoGeneiEG11293. lolB.

Phylogenomic databases

eggNOGiENOG4105C1U. Bacteria.
COG3017. LUCA.
HOGENOMiHOG000119778.
InParanoidiP61320.
KOiK02494.
OMAiYQTRGSF.

Enzyme and pathway databases

BioCyciEcoCyc:EG11293-MONOMER.
ECOL316407:JW1200-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP61320.
PROiP61320.

Family and domain databases

HAMAPiMF_00233. LolB. 1 hit.
InterProiIPR029046. LolA/LolB/LppX.
IPR004565. OM_lipoprot_LolB.
[Graphical view]
PfamiPF03550. LolB. 1 hit.
[Graphical view]
SUPFAMiSSF89392. SSF89392. 1 hit.
TIGRFAMsiTIGR00548. lolB. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLOLB_ECOLI
AccessioniPrimary (citable) accession number: P61320
Secondary accession number(s): P24208, Q46753
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: November 2, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be involved in delta-aminolevulinic acid biosynthesis.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.