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P61320 (LOLB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Outer-membrane lipoprotein LolB
Gene names
Name:lolB
Synonyms:hemM, ychC
Ordered Locus Names:b1209, JW1200
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length207 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the LolA protein. Essential for E.coli viability. HAMAP-Rule MF_00233

Subunit structure

Monomer.

Subcellular location

Cell outer membrane; Lipid-anchor HAMAP-Rule MF_00233.

Sequence similarities

Belongs to the LolB family.

Caution

Was originally (Ref.1) thought to be involved in delta-aminolevulinic acid biosynthesis.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 HAMAP-Rule MF_00233
Chain22 – 207186Outer-membrane lipoprotein LolB HAMAP-Rule MF_00233
PRO_0000018296

Amino acid modifications

Lipidation221N-palmitoyl cysteine Ref.7
Lipidation221S-diacylglycerol cysteine Probable

Experimental info

Sequence conflict121L → V in BAA01105. Ref.1
Sequence conflict41 – 422QH → HD in AAC43435. Ref.3
Sequence conflict176 – 20732YDTKT…NWIVK → MTPKRNLRCQPIWNSPTVVN ASS in AAC43435. Ref.3

Secondary structure

................................ 207
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P61320 [UniParc].

Last modified May 10, 2004. Version 1.
Checksum: C517F6E7353B8551

FASTA20723,551
        10         20         30         40         50         60 
MPLPDFRLIR LLPLAALVLT ACSVTTPKGP GKSPDSPQWR QHQQDVRNLN QYQTRGAFAY 

        70         80         90        100        110        120 
ISDQQKVYAR FFWQQTGQDR YRLLLTNPLG STELELNAQP GNVQLVDNKG QRYTADDAEE 

       130        140        150        160        170        180 
MIGKLTGMPI PLNSLRQWIL GLPGDATDYK LDDQYRLSEI TYSQNGKNWK VVYGGYDTKT 

       190        200 
QPAMPANMEL TDGGQRIKLK MDNWIVK 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of genes involved in the biosynthesis of delta-aminolevulinic acid in Escherichia coli."
Ikemi M., Murakami K., Hashimoto M., Murooka Y.
Gene 121:127-132(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Characterization of the hemA-prs region of the Escherichia coli and Salmonella typhimurium chromosomes: identification of two open reading frames and implications for prs expression."
Post D.A., Hove-Jensen B., Switzer R.L.
J. Gen. Microbiol. 139:259-266(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[3]"Expression of genes kdsA and kdsB involved in 3-deoxy-D-manno-octulosonic acid metabolism and biosynthesis of enterobacterial lipopolysaccharide is growth phase regulated primarily at the transcriptional level in Escherichia coli K-12."
Strohmaier H., Remler P., Renner W., Hoegenauer G.
J. Bacteriol. 177:4488-4500(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[4]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[6]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]"A novel outer membrane lipoprotein, LolB (HemM), involved in the LolA (p20)-dependent localization of lipoproteins to the outer membrane of Escherichia coli."
Matsuyama S., Yokota N., Tokuda H.
EMBO J. 16:6947-6955(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, DIACYLGLYCEROL AT CYS-22, PALMITOYLATION AT CYS-22, PARTIAL PROTEIN SEQUENCE.
[8]"Deletion of lolB, encoding an outer membrane lipoprotein, is lethal for Escherichia coli and causes accumulation of lipoprotein localization intermediates in the periplasm."
Tanaka K., Matsuyama S., Tokuda H.
J. Bacteriol. 183:6538-6542(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: IMPORTANCE FOR VIABILITY.
Strain: K12.
[9]"Crystal structures of bacterial lipoprotein localization factors, LolA and LolB."
Takeda K., Miyatake H., Yokota N., Matsuyama S., Tokuda H., Miki K.
EMBO J. 22:3199-3209(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M77237 Genomic DNA. Translation: AAA24433.1.
D10264 Genomic DNA. Translation: BAA01105.1.
U18555 Genomic DNA. Translation: AAC43435.1.
U00096 Genomic DNA. Translation: AAC74293.1.
AP009048 Genomic DNA. Translation: BAA36067.1.
PIRA47706.
RefSeqNP_415727.1. NC_000913.3.
YP_489476.1. NC_007779.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1IWMX-ray1.90A/B22-207[»]
1IWNX-ray2.20A22-207[»]
ProteinModelPortalP61320.
SMRP61320. Positions 31-207.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-35933N.
IntActP61320. 7 interactions.
MINTMINT-7259909.
STRING511145.b1209.

Protein family/group databases

TCDB1.B.46.1.1. the outer membrane lolab lipoprotein insertion apparatus (lolab) family.

Proteomic databases

PaxDbP61320.
PRIDEP61320.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74293; AAC74293; b1209.
BAA36067; BAA36067; BAA36067.
GeneID12931820.
945775.
KEGGecj:Y75_p1181.
eco:b1209.
PATRIC32117670. VBIEscCol129921_1257.

Organism-specific databases

EchoBASEEB1270.
EcoGeneEG11293. lolB.

Phylogenomic databases

eggNOGCOG3017.
HOGENOMHOG000119778.
KOK02494.
OMAYQTRGSF.
OrthoDBEOG6R5C6M.

Enzyme and pathway databases

BioCycEcoCyc:EG11293-MONOMER.
ECOL316407:JW1200-MONOMER.

Gene expression databases

GenevestigatorP61320.

Family and domain databases

HAMAPMF_00233. LolB.
InterProIPR029046. LolA/LolB/LppX.
IPR004565. OM_lipoprot_LolB.
[Graphical view]
PfamPF03550. LolB. 1 hit.
[Graphical view]
SUPFAMSSF89392. SSF89392. 1 hit.
TIGRFAMsTIGR00548. lolB. 1 hit.
PROSITEPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP61320.
PROP61320.

Entry information

Entry nameLOLB_ECOLI
AccessionPrimary (citable) accession number: P61320
Secondary accession number(s): P24208, Q46753
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: July 9, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene