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Protein

Outer-membrane lipoprotein LolB

Gene

lolB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the LolA protein. Essential for E.coli viability.

Caution

Was originally thought to be involved in delta-aminolevulinic acid biosynthesis.1 Publication

GO - Molecular functioni

  • lipopeptide binding Source: EcoCyc

GO - Biological processi

  • lipid localization Source: EcoCyc
  • lipoprotein localization to outer membrane Source: CACAO
  • lipoprotein metabolic process Source: EcoCyc
  • localization within membrane Source: EcoCyc
  • protein transport Source: UniProtKB-KW

Keywordsi

Molecular functionChaperone
Biological processProtein transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG11293-MONOMER

Protein family/group databases

TCDBi1.B.46.1.1 the outer membrane lolab lipoprotein insertion apparatus (lolab) family

Names & Taxonomyi

Protein namesi
Recommended name:
Outer-membrane lipoprotein LolB
Gene namesi
Name:lolB
Synonyms:hemM, ychC
Ordered Locus Names:b1209, JW1200
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11293 lolB

Subcellular locationi

GO - Cellular componenti

  • cell outer membrane Source: EcoCyc

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Chemistry databases

DrugBankiDB02078 1-Methoxy-2-[2-(2-Methoxy-Ethoxy]-Ethane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Add BLAST21
ChainiPRO_000001829622 – 207Outer-membrane lipoprotein LolBAdd BLAST186

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi22N-palmitoyl cysteine1 Publication1
Lipidationi22S-diacylglycerol cysteine1 Publication1

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiP61320
PRIDEiP61320

Interactioni

Subunit structurei

Monomer.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi4260814, 301 interactors
DIPiDIP-35933N
IntActiP61320, 8 interactors
MINTiP61320
STRINGi316385.ECDH10B_1262

Structurei

Secondary structure

1207
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi34 – 36Combined sources3
Helixi37 – 47Combined sources11
Beta strandi51 – 61Combined sources11
Beta strandi66 – 76Combined sources11
Turni77 – 79Combined sources3
Beta strandi80 – 86Combined sources7
Beta strandi92 – 99Combined sources8
Beta strandi102 – 106Combined sources5
Beta strandi112 – 116Combined sources5
Helixi118 – 126Combined sources9
Helixi132 – 138Combined sources7
Turni139 – 141Combined sources3
Beta strandi149 – 151Combined sources3
Beta strandi157 – 166Combined sources10
Beta strandi168 – 173Combined sources6
Beta strandi180 – 182Combined sources3
Beta strandi187 – 191Combined sources5
Beta strandi196 – 207Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IWMX-ray1.90A/B22-207[»]
1IWNX-ray2.20A22-207[»]
ProteinModelPortaliP61320
SMRiP61320
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61320

Family & Domainsi

Sequence similaritiesi

Belongs to the LolB family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105C1U Bacteria
COG3017 LUCA
HOGENOMiHOG000119778
InParanoidiP61320
KOiK02494
OMAiLKMDNWT

Family and domain databases

CDDicd16326 LolB, 1 hit
HAMAPiMF_00233 LolB, 1 hit
InterProiView protein in InterPro
IPR029046 LolA/LolB/LppX
IPR004565 OM_lipoprot_LolB
PfamiView protein in Pfam
PF03550 LolB, 1 hit
SUPFAMiSSF89392 SSF89392, 1 hit
TIGRFAMsiTIGR00548 lolB, 1 hit
PROSITEiView protein in PROSITE
PS51257 PROKAR_LIPOPROTEIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61320-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLPDFRLIR LLPLAALVLT ACSVTTPKGP GKSPDSPQWR QHQQDVRNLN
60 70 80 90 100
QYQTRGAFAY ISDQQKVYAR FFWQQTGQDR YRLLLTNPLG STELELNAQP
110 120 130 140 150
GNVQLVDNKG QRYTADDAEE MIGKLTGMPI PLNSLRQWIL GLPGDATDYK
160 170 180 190 200
LDDQYRLSEI TYSQNGKNWK VVYGGYDTKT QPAMPANMEL TDGGQRIKLK

MDNWIVK
Length:207
Mass (Da):23,551
Last modified:May 10, 2004 - v1
Checksum:iC517F6E7353B8551
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12L → V in BAA01105 (PubMed:1427085).Curated1
Sequence conflicti41 – 42QH → HD in AAC43435 (PubMed:7543480).Curated2
Sequence conflicti176 – 207YDTKT…NWIVK → MTPKRNLRCQPIWNSPTVVN ASS in AAC43435 (PubMed:7543480).CuratedAdd BLAST32

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77237 Genomic DNA Translation: AAA24433.1
D10264 Genomic DNA Translation: BAA01105.1
U18555 Genomic DNA Translation: AAC43435.1
U00096 Genomic DNA Translation: AAC74293.1
AP009048 Genomic DNA Translation: BAA36067.1
PIRiA47706
RefSeqiNP_415727.1, NC_000913.3
WP_001130692.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74293; AAC74293; b1209
BAA36067; BAA36067; BAA36067
GeneIDi945775
KEGGiecj:JW1200
eco:b1209
PATRICifig|1411691.4.peg.1075

Similar proteinsi

Entry informationi

Entry nameiLOLB_ECOLI
AccessioniPrimary (citable) accession number: P61320
Secondary accession number(s): P24208, Q46753
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: March 28, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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