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Protein

Outer-membrane lipoprotein carrier protein

Gene

lolA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Participates in the translocation of lipoproteins from the inner membrane to the outer membrane. Only forms a complex with a lipoprotein if the residue after the N-terminal Cys is not an aspartate (The Asp acts as a targeting signal to indicate that the lipoprotein should stay in the inner membrane); the inner membrane retention signal functions at the release step.1 Publication
May act as a regulator of the RCS-phosphorelay signal transduction pathway.1 Publication

GO - Molecular functioni

  • lipoprotein transporter activity Source: EcoCyc
  • protein transporter activity Source: UniProtKB-HAMAP

GO - Biological processi

  • chaperone-mediated protein transport across periplasmic space Source: CACAO
  • lipoprotein localization to outer membrane Source: CACAO
  • lipoprotein transport Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:G6465-MONOMER.
ECOL316407:JW0874-MONOMER.

Protein family/group databases

TCDBi1.B.46.1.1. the outer membrane lolab lipoprotein insertion apparatus (lolab) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Outer-membrane lipoprotein carrier protein
Alternative name(s):
P20
Gene namesi
Name:lolA
Synonyms:lplA, yzzV
Ordered Locus Names:b0891, JW0874
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12684. lolA.

Subcellular locationi

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi64R → L: Loss of ability to transfer lipoproteins to LolB. 1 Publication1
Mutagenesisi68F → E: Loss of ability to bind lipoproteins. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 212 PublicationsAdd BLAST21
ChainiPRO_000001825622 – 203Outer-membrane lipoprotein carrier proteinAdd BLAST182

Proteomic databases

EPDiP61316.
PaxDbiP61316.
PRIDEiP61316.

2D gel databases

SWISS-2DPAGEP61316.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4260649. 271 interactors.
DIPiDIP-35675N.
IntActiP61316. 11 interactors.
MINTiMINT-1249033.
STRINGi511145.b0891.

Structurei

Secondary structure

1203
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi23 – 30Combined sources8
Beta strandi35 – 46Combined sources12
Beta strandi48 – 51Combined sources4
Beta strandi54 – 63Combined sources10
Turni64 – 66Combined sources3
Beta strandi67 – 72Combined sources6
Beta strandi74 – 76Combined sources3
Beta strandi78 – 91Combined sources14
Helixi92 – 94Combined sources3
Beta strandi96 – 101Combined sources6
Helixi102 – 104Combined sources3
Turni105 – 107Combined sources3
Helixi110 – 116Combined sources7
Helixi119 – 122Combined sources4
Beta strandi125 – 130Combined sources6
Beta strandi133 – 142Combined sources10
Beta strandi144 – 152Combined sources9
Beta strandi158 – 165Combined sources8
Beta strandi170 – 181Combined sources12
Helixi185 – 188Combined sources4
Beta strandi197 – 200Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IWLX-ray1.65A22-203[»]
1UA8X-ray1.90A22-203[»]
2ZPCX-ray2.35A22-203[»]
2ZPDX-ray1.85A22-203[»]
3KSNX-ray1.65A22-203[»]
ProteinModelPortaliP61316.
SMRiP61316.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61316.

Family & Domainsi

Sequence similaritiesi

Belongs to the LolA family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107Y7W. Bacteria.
COG2834. LUCA.
HOGENOMiHOG000257501.
InParanoidiP61316.
KOiK03634.
OMAiYDPFVEQ.
PhylomeDBiP61316.

Family and domain databases

HAMAPiMF_00240. LolA. 1 hit.
InterProiIPR029046. LolA/LolB/LppX.
IPR004564. OM_lipoprot_carrier_LolA-like.
IPR018323. OM_lipoprot_carrier_LolA_Pbac.
[Graphical view]
PfamiPF03548. LolA. 1 hit.
[Graphical view]
SUPFAMiSSF89392. SSF89392. 1 hit.
TIGRFAMsiTIGR00547. lolA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61316-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKIAITCAL LSSLVASSVW ADAASDLKSR LDKVSSFHAS FTQKVTDGSG
60 70 80 90 100
AAVQEGQGDL WVKRPNLFNW HMTQPDESIL VSDGKTLWFY NPFVEQATAT
110 120 130 140 150
WLKDATGNTP FMLIARNQSS DWQQYNIKQN GDDFVLTPKA SNGNLKQFTI
160 170 180 190 200
NVGRDGTIHQ FSAVEQDDQR SSYQLKSQQN GAVDAAKFTF TPPQGVTVDD

QRK
Length:203
Mass (Da):22,497
Last modified:May 10, 2004 - v1
Checksum:iF2884D82D8DFEF1D
GO

Sequence cautioni

The sequence BAA08390 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAA35616 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49398 Genomic DNA. Translation: BAA08390.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73977.2.
AP009048 Genomic DNA. Translation: BAA35616.1. Different initiation.
PIRiS57828.
RefSeqiNP_415411.2. NC_000913.3.
WP_001295343.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73977; AAC73977; b0891.
BAA35616; BAA35616; BAA35616.
GeneIDi948989.
KEGGiecj:JW0874.
eco:b0891.
PATRICi32116993. VBIEscCol129921_0921.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49398 Genomic DNA. Translation: BAA08390.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73977.2.
AP009048 Genomic DNA. Translation: BAA35616.1. Different initiation.
PIRiS57828.
RefSeqiNP_415411.2. NC_000913.3.
WP_001295343.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IWLX-ray1.65A22-203[»]
1UA8X-ray1.90A22-203[»]
2ZPCX-ray2.35A22-203[»]
2ZPDX-ray1.85A22-203[»]
3KSNX-ray1.65A22-203[»]
ProteinModelPortaliP61316.
SMRiP61316.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260649. 271 interactors.
DIPiDIP-35675N.
IntActiP61316. 11 interactors.
MINTiMINT-1249033.
STRINGi511145.b0891.

Protein family/group databases

TCDBi1.B.46.1.1. the outer membrane lolab lipoprotein insertion apparatus (lolab) family.

2D gel databases

SWISS-2DPAGEP61316.

Proteomic databases

EPDiP61316.
PaxDbiP61316.
PRIDEiP61316.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73977; AAC73977; b0891.
BAA35616; BAA35616; BAA35616.
GeneIDi948989.
KEGGiecj:JW0874.
eco:b0891.
PATRICi32116993. VBIEscCol129921_0921.

Organism-specific databases

EchoBASEiEB2548.
EcoGeneiEG12684. lolA.

Phylogenomic databases

eggNOGiENOG4107Y7W. Bacteria.
COG2834. LUCA.
HOGENOMiHOG000257501.
InParanoidiP61316.
KOiK03634.
OMAiYDPFVEQ.
PhylomeDBiP61316.

Enzyme and pathway databases

BioCyciEcoCyc:G6465-MONOMER.
ECOL316407:JW0874-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP61316.
PROiP61316.

Family and domain databases

HAMAPiMF_00240. LolA. 1 hit.
InterProiIPR029046. LolA/LolB/LppX.
IPR004564. OM_lipoprot_carrier_LolA-like.
IPR018323. OM_lipoprot_carrier_LolA_Pbac.
[Graphical view]
PfamiPF03548. LolA. 1 hit.
[Graphical view]
SUPFAMiSSF89392. SSF89392. 1 hit.
TIGRFAMsiTIGR00547. lolA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLOLA_ECOLI
AccessioniPrimary (citable) accession number: P61316
Secondary accession number(s): P39178, Q8X5H8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: November 2, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.