P61313 (RL15_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
60S ribosomal protein L15

Gene names

Name:	RPL15
Synonyms:	EC45
ORF Names:	TCBAP0781

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	204 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Interacts with IFIT1 (via TPR repeats 1-4). Ref.14
Sequence similarities	Belongs to the ribosomal protein L15e family.
Sequence caution	The sequence AAA36583.1 differs from that shown. Reason: Frameshift at position 199.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	SRP-dependent cotranslational protein targeting to membrane Traceable author statement. Source: Reactome nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Traceable author statement. Source: Reactome translational elongation Traceable author statement. Source: Reactome translational initiation Traceable author statement. Source: Reactome translational termination Traceable author statement. Source: Reactome viral transcription Traceable author statement. Source: Reactome
Cellular_component	cytosolic large ribosomal subunit Inferred from direct assay PubMed 12962325. Source: UniProtKB
Molecular_function	RNA binding Traceable author statement PubMed 9582194. Source: ProtInc structural constituent of ribosome Non-traceable author statement PubMed 12962325. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P61313-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P61313-2)

The sequence of this isoform differs from the canonical sequence as follows:
     121-204: VGEDSTYKFF...RNTLQLHRYR → MESHSHSGGSAVGQSRLTATSVSRV

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 204	204	60S ribosomal protein L15		PRO_0000127527
Amino acid modifications
	Modified residue	97	1	Phosphoserine Ref.11 Ref.15
	Modified residue	100	1	Phosphoserine Ref.12
Natural variations
	Alternative sequence	121 – 204	84	VGEDS…LHRYR → MESHSHSGGSAVGQSRLTAT SVSRV in isoform 2.		VSP_045144
Experimental info
	Sequence conflict	101	1	V → L in AAG44837. Ref.5
	Sequence conflict	129	1	F → A in AAA36583. Ref.1
	Sequence conflict	129	1	F → A in BAB79461. Ref.3
	Sequence conflict	155	1	V → D in AAA36583. Ref.1
	Sequence conflict	180	1	F → S in AAH14837. Ref.10

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: A6E3AD1A76C9506F	FASTA	204	24,146
	10 20 30 40 50 60 MGAYKYIQEL WRKKQSDVMR FLLRVRCWQY RQLSALHRAP RPTRPDKARR LGYKAKQGYV 70 80 90 100 110 120 IYRIRVRRGG RKRPVPKGAT YGKPVHHGVN QLKFARSLQS VAEERAGRHC GALRVLNSYW 130 140 150 160 170 180 VGEDSTYKFF EVILIDPFHK AIRRNPDTQW ITKPVHKHRE MRGLTSAGRK SRGLGKGHKF 190 200 HHTIGGSRRA AWRRRNTLQL HRYR
	Isoform 2 [UniParc]. Checksum: 0195D5816021FCC4	FASTA	145	16,717
	10 20 30 40 50 60 MGAYKYIQEL WRKKQSDVMR FLLRVRCWQY RQLSALHRAP RPTRPDKARR LGYKAKQGYV 70 80 90 100 110 120 IYRIRVRRGG RKRPVPKGAT YGKPVHHGVN QLKFARSLQS VAEERAGRHC GALRVLNSYW 130 140 MESHSHSGGS AVGQSRLTAT SVSRV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA encoding the human homologue of yeast ribosomal protein YL10." Herzog H. Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Cloning and characterization of EC45 gene, encoding human ribosomal protein L15 and overexpressing in esophageal cancer." Wang Q., Yang C., Zhou J., Liu Z., Wang X., Zhou C., Wu M. Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Structures of 69 human ribosomal protein genes: cloning, sequencing, and comparative analysis." Yoshihama M., Uechi T., Asakawa S., Kawasaki K., Kato S., Higa S., Maeda N., Tanaka T., Shimizu N., Kenmochi N. Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Cloning of a new isoform of ribosomal protein L15 in testis." Lu L., Huang X.Y., Xu M., Yin L.L., Li J.M., Zhou Z.M., Sha J.H. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Testis.
[5]	"Pediatric leukemia cDNA sequencing project." Villalon D.K., Luna R.A., Margolin J.K., Tsang Y.T.M., Hale S.M., Mei G., Bouck J., Gibbs R.A. Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Leukemia.
[6]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lung and Small intestine.
[7]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[8]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	"The DNA sequence, annotation and analysis of human chromosome 3." Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A. Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[10]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Bone, Brain, Cervix and Skin.
[11]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-97, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-100, MASS SPECTROMETRY.
[13]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]	"A novel interaction between interferon-inducible protein p56 and ribosomal protein L15 in gastric cancer cells." Hsu Y.A., Lin H.J., Sheu J.J., Shieh F.K., Chen S.Y., Lai C.H., Tsai F.J., Wan L., Chen B.H. DNA Cell Biol. 30:671-679(2011) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH IFIT1.
[15]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-97, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L25899 mRNA. Translation: AAA36583.1. Frameshift. AF279903 mRNA. Translation: AAG44837.1. AB061823 Genomic DNA. Translation: BAB79461.1. AY347528 mRNA. Translation: AAQ24859.1. AF283772 mRNA. Translation: AAG15591.1. AK290694 mRNA. Translation: BAF83383.1. AK292000 mRNA. Translation: BAF84689.1. BT019672 mRNA. Translation: AAV38478.1. AC124914 Genomic DNA. No translation available. CH471055 Genomic DNA. Translation: EAW64338.1. BC081565 mRNA. Translation: AAH81565.1. BC014837 mRNA. Translation: AAH14837.1. BC068198 mRNA. Translation: AAH68198.1. BC070328 mRNA. Translation: AAH70328.1. BC071672 mRNA. Translation: AAH71672.1.
IPI	IPI00470528.
RefSeq	NP_001240308.1. NM_001253379.1. NP_001240309.1. NM_001253380.1. NP_001240311.1. NM_001253382.1. NP_001240312.1. NM_001253383.1. NP_001240313.1. NM_001253384.1. NP_002939.2. NM_002948.3.
UniGene	Hs.381219. Hs.592455.
3D structure databases
ProteinModelPortal	P61313.
ModBase	Search...
Protein-protein interaction databases
IntAct	P61313. 14 interactions.
STRING	9606.ENSP00000309334.
PTM databases
PhosphoSite	P61313.
Polymorphism databases
DMDM	47117726.
2D gel databases
SWISS-2DPAGE	P61313.
Proteomic databases
PaxDb	P61313.
PRIDE	P61313.
Protocols and materials databases
DNASU	6138.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000307839; ENSP00000309334; ENSG00000174748. ENST00000354811; ENSP00000346867; ENSG00000174748. ENST00000413699; ENSP00000416692; ENSG00000174748. ENST00000415719; ENSP00000388529; ENSG00000174748. ENST00000435882; ENSP00000390193; ENSG00000174748. ENST00000456530; ENSP00000398788; ENSG00000174748.
GeneID	6138.
KEGG	hsa:6138.
UCSC	uc003ccn.3. human.
Organism-specific databases
CTD	6138.
GeneCards	GC03P023933.
HGNC	HGNC:10306. RPL15.
HPA	HPA003511.
MIM	604174. gene.
neXtProt	NX_P61313.
PharmGKB	PA34674.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1632.
HOVERGEN	HBG000443.
InParanoid	P61313.
KO	K02877.
OMA	SRRAAWK.
OrthoDB	EOG4W3SP2.
Enzyme and pathway databases
Reactome	REACT_116125. Disease. REACT_17015. Metabolism of proteins. REACT_1762. 3' -UTR-mediated translational regulation. REACT_21257. Metabolism of RNA. REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	P61313.
Bgee	P61313.
CleanEx	HS_RPL15.
Genevestigator	P61313.
GermOnline	ENSG00000174748. Homo sapiens.
Family and domain databases
Gene3D	3.40.1120.10. 1 hit.
InterPro	IPR024794. Rbsml_L15e_core_dom. IPR000439. Ribosomal_L15e. IPR020925. Ribosomal_L15e_CS. IPR012678. Ribosomal_L23/L15e_core_dom. [Graphical view]
PANTHER	PTHR11847. PTHR11847. 1 hit.
Pfam	PF00827. Ribosomal_L15e. 1 hit. [Graphical view]
SUPFAM	SSF54189. L23_L15e_core. 1 hit.
PROSITE	PS01194. RIBOSOMAL_L15E. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	6138.
NextBio	23845.
SOURCE	Search...

Entry information

Entry name

RL15_HUMAN

Accession

Primary (citable) accession number: P61313
Secondary accession number(s): P39030 , P41051, Q5U0C0, Q642I1, Q6IPX6, Q8WYP2, Q96C44, Q9H2E5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.