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P61290

- PSME3_MOUSE

UniProt

P61290 - PSME3_MOUSE

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Protein

Proteasome activator complex subunit 3

Gene

Psme3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation (By similarity).By similarity

GO - Molecular functioni

  1. endopeptidase activator activity Source: Ensembl
  2. MDM2/MDM4 family protein binding Source: UniProtKB
  3. p53 binding Source: UniProtKB

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. cell cycle Source: UniProtKB-KW
  3. negative regulation of extrinsic apoptotic signaling pathway Source: UniProtKB
  4. regulation of proteasomal protein catabolic process Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Apoptosis, Cell cycle

Enzyme and pathway databases

ReactomeiREACT_198693. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_199105. ER-Phagosome pathway.
REACT_199114. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_199115. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_199121. Activation of NF-kappaB in B cells.
REACT_203336. Degradation of beta-catenin by the destruction complex.
REACT_203517. SCF-beta-TrCP mediated degradation of Emi1.
REACT_203973. Asymmetric localization of PCP proteins.
REACT_207679. Separation of Sister Chromatids.
REACT_212633. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_218318. Regulation of activated PAK-2p34 by proteasome mediated degradation.
REACT_219129. degradation of AXIN.
REACT_219897. APC/C:Cdc20 mediated degradation of Securin.
REACT_220647. SCF(Skp2)-mediated degradation of p27/p21.
REACT_225686. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_226135. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_227429. degradation of DVL.
REACT_232069. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_232469. Hh ligand biogenesis disease.
REACT_233316. Hedgehog ligand biogenesis.
REACT_234963. Regulation of ornithine decarboxylase (ODC).
REACT_244558. CDT1 association with the CDC6:ORC:origin complex.
REACT_245230. Orc1 removal from chromatin.
REACT_249922. CDK-mediated phosphorylation and removal of Cdc6.
REACT_263467. Ubiquitin-dependent degradation of Cyclin D1.
REACT_268522. GLI3 is processed to GLI3R by the proteasome.
REACT_268705. Degradation of GLI1 by the proteasome.
REACT_270923. Degradation of GLI2 by the proteasome.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome activator complex subunit 3
Alternative name(s):
11S regulator complex subunit gamma
Short name:
REG-gamma
Activator of multicatalytic protease subunit 3
Ki nuclear autoantigen
Proteasome activator 28 subunit gamma
Short name:
PA28g
Short name:
PA28gamma
Gene namesi
Name:Psme3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:1096366. Psme3.

Subcellular locationi

Nucleus 1 Publication. Cytoplasm 1 Publication
Note: Localizes to the cytoplasm during mitosis following nuclear envelope breakdown at this distinct stage of the cell cycle which allows its interaction with MAP3K3 kinase.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
  3. proteasome activator complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

Pathology & Biotechi

Disruption phenotypei

Growth retardation, small body size, and reduces significantly the percentage of cells that enter the S phase.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 254253Proteasome activator complex subunit 3PRO_0000161790Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei24 – 241PhosphoserineBy similarity
Modified residuei195 – 1951N6-acetyllysine; by P300/CBPBy similarity

Post-translational modificationi

Phosphorylated by MAP3K3.1 Publication
Acetylation at the major site Lys-195 is important for oligomerization and ability to degrade its target substrates. Deacetylated by SIRT1 (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP61290.
PaxDbiP61290.
PRIDEiP61290.

2D gel databases

REPRODUCTION-2DPAGEP61290.

PTM databases

PhosphoSiteiP61290.

Expressioni

Gene expression databases

BgeeiP61290.
CleanExiMM_PSME3.
ExpressionAtlasiP61290. baseline and differential.
GenevestigatoriP61290.

Interactioni

Subunit structurei

Homoheptamer; the stability of the heptamer is essential for the specific activation of the trypsine-like subunit and inhibition of the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits of the proteasome (By similarity). Interacts with p53/TP53 and MDM2 (By similarity). Interacts with MAP3K3. Associates with the proteasome (By similarity).By similarity

Protein-protein interaction databases

BioGridi202435. 5 interactions.
IntActiP61290. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliP61290.
SMRiP61290. Positions 5-64, 112-246.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The C-terminal sequences affect heptamer stability and proteasome affinity.By similarity

Sequence similaritiesi

Belongs to the PA28 family.Curated

Phylogenomic databases

eggNOGiNOG236353.
HOGENOMiHOG000282822.
HOVERGENiHBG053745.
InParanoidiP61290.
KOiK06698.
OMAiFVMPGGM.
PhylomeDBiP61290.
TreeFamiTF106236.

Family and domain databases

Gene3Di1.20.120.180. 1 hit.
1.20.5.120. 1 hit.
InterProiIPR009077. Proteasome_activ_pa28.
IPR003186. Proteasome_activ_pa28_C.
IPR003185. Proteasome_activ_pa28_N.
[Graphical view]
PANTHERiPTHR10660. PTHR10660. 1 hit.
PfamiPF02251. PA28_alpha. 1 hit.
PF02252. PA28_beta. 1 hit.
[Graphical view]
SUPFAMiSSF47216. SSF47216. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61290-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASLLKVDQE VKLKVDSFRE RITSEAEDLV ANFFPKKLLE LDSFLKEPIL
60 70 80 90 100
NIHDLTQIHS DMNLPVPDPI LLTNSHDGLD GPTYKKRRLD ECEEAFQGTK
110 120 130 140 150
VFVMPNGMLK SNQQLVDIIE KVKPEIRLLI EKCNTVKMWV QLLIPRIEDG
160 170 180 190 200
NNFGVSIQEE TVAELRTVES EAASYLDQIS RYYITRAKLV SKIAKYPHVE
210 220 230 240 250
DYRRTVTEID EKEYISLRLI ISELRNQYVT LHDMILKNIE KIKRPRSSNA

ETLY
Length:254
Mass (Da):29,506
Last modified:May 10, 2004 - v1
Checksum:i116FAB47D60A26C0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21A → L in AAC53297. (PubMed:9162094)Curated
Sequence conflicti9 – 91Q → L in AAC53297. (PubMed:9162094)Curated
Sequence conflicti23 – 231T → S in AAC53297. (PubMed:9162094)Curated
Sequence conflicti117 – 1171D → G in AAC53297. (PubMed:9162094)Curated
Sequence conflicti172 – 1721A → P in AAC53297. (PubMed:9162094)Curated
Sequence conflicti188 – 1881K → G in AAC53297. (PubMed:9162094)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87911 mRNA. Translation: BAA22041.1.
U60330 mRNA. Translation: AAC53297.1.
AB007139 Genomic DNA. Translation: BAA28838.1.
BC015255 mRNA. Translation: AAH15255.1.
CCDSiCCDS25463.1.
RefSeqiNP_035322.1. NM_011192.3.
UniGeneiMm.288477.

Genome annotation databases

EnsembliENSMUST00000019470; ENSMUSP00000019470; ENSMUSG00000078652.
GeneIDi19192.
KEGGimmu:19192.
UCSCiuc007lon.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87911 mRNA. Translation: BAA22041.1 .
U60330 mRNA. Translation: AAC53297.1 .
AB007139 Genomic DNA. Translation: BAA28838.1 .
BC015255 mRNA. Translation: AAH15255.1 .
CCDSi CCDS25463.1.
RefSeqi NP_035322.1. NM_011192.3.
UniGenei Mm.288477.

3D structure databases

ProteinModelPortali P61290.
SMRi P61290. Positions 5-64, 112-246.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202435. 5 interactions.
IntActi P61290. 2 interactions.

PTM databases

PhosphoSitei P61290.

2D gel databases

REPRODUCTION-2DPAGE P61290.

Proteomic databases

MaxQBi P61290.
PaxDbi P61290.
PRIDEi P61290.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000019470 ; ENSMUSP00000019470 ; ENSMUSG00000078652 .
GeneIDi 19192.
KEGGi mmu:19192.
UCSCi uc007lon.1. mouse.

Organism-specific databases

CTDi 10197.
MGIi MGI:1096366. Psme3.

Phylogenomic databases

eggNOGi NOG236353.
HOGENOMi HOG000282822.
HOVERGENi HBG053745.
InParanoidi P61290.
KOi K06698.
OMAi FVMPGGM.
PhylomeDBi P61290.
TreeFami TF106236.

Enzyme and pathway databases

Reactomei REACT_198693. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_199105. ER-Phagosome pathway.
REACT_199114. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_199115. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_199121. Activation of NF-kappaB in B cells.
REACT_203336. Degradation of beta-catenin by the destruction complex.
REACT_203517. SCF-beta-TrCP mediated degradation of Emi1.
REACT_203973. Asymmetric localization of PCP proteins.
REACT_207679. Separation of Sister Chromatids.
REACT_212633. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_218318. Regulation of activated PAK-2p34 by proteasome mediated degradation.
REACT_219129. degradation of AXIN.
REACT_219897. APC/C:Cdc20 mediated degradation of Securin.
REACT_220647. SCF(Skp2)-mediated degradation of p27/p21.
REACT_225686. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_226135. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_227429. degradation of DVL.
REACT_232069. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_232469. Hh ligand biogenesis disease.
REACT_233316. Hedgehog ligand biogenesis.
REACT_234963. Regulation of ornithine decarboxylase (ODC).
REACT_244558. CDT1 association with the CDC6:ORC:origin complex.
REACT_245230. Orc1 removal from chromatin.
REACT_249922. CDK-mediated phosphorylation and removal of Cdc6.
REACT_263467. Ubiquitin-dependent degradation of Cyclin D1.
REACT_268522. GLI3 is processed to GLI3R by the proteasome.
REACT_268705. Degradation of GLI1 by the proteasome.
REACT_270923. Degradation of GLI2 by the proteasome.

Miscellaneous databases

ChiTaRSi Psme3. mouse.
NextBioi 295900.
PROi P61290.
SOURCEi Search...

Gene expression databases

Bgeei P61290.
CleanExi MM_PSME3.
ExpressionAtlasi P61290. baseline and differential.
Genevestigatori P61290.

Family and domain databases

Gene3Di 1.20.120.180. 1 hit.
1.20.5.120. 1 hit.
InterProi IPR009077. Proteasome_activ_pa28.
IPR003186. Proteasome_activ_pa28_C.
IPR003185. Proteasome_activ_pa28_N.
[Graphical view ]
PANTHERi PTHR10660. PTHR10660. 1 hit.
Pfami PF02251. PA28_alpha. 1 hit.
PF02252. PA28_beta. 1 hit.
[Graphical view ]
SUPFAMi SSF47216. SSF47216. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "PA28 subunits of the mouse proteasome: primary structures and chromosomal localization of the genes."
    Kandil E., Kohda K., Ishibashi T., Tanaka K., Kasahara M.
    Immunogenetics 46:337-344(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Spleen.
  2. "Sequence and expression of mouse proteasome activator PA28 and the related autoantigen Ki."
    Jiang H., Monaco J.J.
    Immunogenetics 46:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: B10.BR.
  3. "Characterization of the mouse PA28 activator complex gene family: complete organizations of the three member genes and a physical map of the approximately 150-kb region containing the alpha- and beta-subunit genes."
    Kohda K., Ishibashi T., Shimbara N., Tanaka K., Matsuda Y., Kasahara M.
    J. Immunol. 160:4923-4935(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJ.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Kidney.
  5. "Growth retardation in mice lacking the proteasome activator PA28gamma."
    Murata S., Kawahara H., Tohma S., Yamamoto K., Kasahara M., Nabeshima Y., Tanaka K., Chiba T.
    J. Biol. Chem. 274:38211-38215(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  6. "MEKK3 interacts with the PA28 gamma regulatory subunit of the proteasome."
    Hagemann C., Patel R., Blank J.L.
    Biochem. J. 373:71-79(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MAP3K3, PHOSPHORYLATION BY MAP3K3, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiPSME3_MOUSE
AccessioniPrimary (citable) accession number: P61290
Secondary accession number(s): O35563
, P97373, Q12920, Q13172, Q9BQD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: November 26, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3