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P61290

- PSME3_MOUSE

UniProt

P61290 - PSME3_MOUSE

Protein

Proteasome activator complex subunit 3

Gene

Psme3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (10 May 2004)
      Previous versions | rss
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    Functioni

    Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation By similarity.By similarity

    GO - Molecular functioni

    1. endopeptidase activator activity Source: Ensembl
    2. MDM2/MDM4 family protein binding Source: UniProtKB
    3. p53 binding Source: UniProtKB

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW
    2. cell cycle Source: UniProtKB-KW
    3. negative regulation of extrinsic apoptotic signaling pathway Source: UniProtKB
    4. regulation of proteasomal protein catabolic process Source: Ensembl

    Keywords - Biological processi

    Apoptosis, Cell cycle

    Enzyme and pathway databases

    ReactomeiREACT_198693. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_199105. ER-Phagosome pathway.
    REACT_199114. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_199115. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_199121. Activation of NF-kappaB in B cells.
    REACT_203517. SCF-beta-TrCP mediated degradation of Emi1.
    REACT_203973. Asymmetric localization of PCP proteins.
    REACT_207679. Separation of Sister Chromatids.
    REACT_212633. Autodegradation of the E3 ubiquitin ligase COP1.
    REACT_218318. Regulation of activated PAK-2p34 by proteasome mediated degradation.
    REACT_219129. degradation of AXIN.
    REACT_219897. APC/C:Cdc20 mediated degradation of Securin.
    REACT_220647. SCF(Skp2)-mediated degradation of p27/p21.
    REACT_225686. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_226135. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
    REACT_227429. degradation of DVL.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteasome activator complex subunit 3
    Alternative name(s):
    11S regulator complex subunit gamma
    Short name:
    REG-gamma
    Activator of multicatalytic protease subunit 3
    Ki nuclear autoantigen
    Proteasome activator 28 subunit gamma
    Short name:
    PA28g
    Short name:
    PA28gamma
    Gene namesi
    Name:Psme3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1096366. Psme3.

    Subcellular locationi

    Nucleus 1 Publication. Cytoplasm 1 Publication
    Note: Localizes to the cytoplasm during mitosis following nuclear envelope breakdown at this distinct stage of the cell cycle which allows its interaction with MAP3K3 kinase.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell
    3. proteasome activator complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Proteasome

    Pathology & Biotechi

    Disruption phenotypei

    Growth retardation, small body size, and reduces significantly the percentage of cells that enter the S phase.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 254253Proteasome activator complex subunit 3PRO_0000161790Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei24 – 241PhosphoserineBy similarity
    Modified residuei195 – 1951N6-acetyllysine; by P300/CBPBy similarity

    Post-translational modificationi

    Phosphorylated by MAP3K3.1 Publication
    Acetylation at the major site Lys-195 is important for oligomerization and ability to degrade its target substrates. Deacetylated by SIRT1 By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP61290.
    PaxDbiP61290.
    PRIDEiP61290.

    2D gel databases

    REPRODUCTION-2DPAGEP61290.

    PTM databases

    PhosphoSiteiP61290.

    Expressioni

    Gene expression databases

    ArrayExpressiP61290.
    BgeeiP61290.
    CleanExiMM_PSME3.
    GenevestigatoriP61290.

    Interactioni

    Subunit structurei

    Homoheptamer; the stability of the heptamer is essential for the specific activation of the trypsine-like subunit and inhibition of the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits of the proteasome By similarity. Interacts with p53/TP53 and MDM2 By similarity. Interacts with MAP3K3. Associates with the proteasome By similarity.By similarity

    Protein-protein interaction databases

    BioGridi202435. 5 interactions.
    IntActiP61290. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP61290.
    SMRiP61290. Positions 5-64, 112-246.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    The C-terminal sequences affect heptamer stability and proteasome affinity.By similarity

    Sequence similaritiesi

    Belongs to the PA28 family.Curated

    Phylogenomic databases

    eggNOGiNOG236353.
    HOGENOMiHOG000282822.
    HOVERGENiHBG053745.
    KOiK06698.
    OMAiFVMPGGM.
    PhylomeDBiP61290.
    TreeFamiTF106236.

    Family and domain databases

    Gene3Di1.20.120.180. 1 hit.
    1.20.5.120. 1 hit.
    InterProiIPR009077. Proteasome_activ_pa28.
    IPR003186. Proteasome_activ_pa28_C.
    IPR003185. Proteasome_activ_pa28_N.
    [Graphical view]
    PANTHERiPTHR10660. PTHR10660. 1 hit.
    PfamiPF02251. PA28_alpha. 1 hit.
    PF02252. PA28_beta. 1 hit.
    [Graphical view]
    SUPFAMiSSF47216. SSF47216. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P61290-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASLLKVDQE VKLKVDSFRE RITSEAEDLV ANFFPKKLLE LDSFLKEPIL    50
    NIHDLTQIHS DMNLPVPDPI LLTNSHDGLD GPTYKKRRLD ECEEAFQGTK 100
    VFVMPNGMLK SNQQLVDIIE KVKPEIRLLI EKCNTVKMWV QLLIPRIEDG 150
    NNFGVSIQEE TVAELRTVES EAASYLDQIS RYYITRAKLV SKIAKYPHVE 200
    DYRRTVTEID EKEYISLRLI ISELRNQYVT LHDMILKNIE KIKRPRSSNA 250
    ETLY 254
    Length:254
    Mass (Da):29,506
    Last modified:May 10, 2004 - v1
    Checksum:i116FAB47D60A26C0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti2 – 21A → L in AAC53297. (PubMed:9162094)Curated
    Sequence conflicti9 – 91Q → L in AAC53297. (PubMed:9162094)Curated
    Sequence conflicti23 – 231T → S in AAC53297. (PubMed:9162094)Curated
    Sequence conflicti117 – 1171D → G in AAC53297. (PubMed:9162094)Curated
    Sequence conflicti172 – 1721A → P in AAC53297. (PubMed:9162094)Curated
    Sequence conflicti188 – 1881K → G in AAC53297. (PubMed:9162094)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D87911 mRNA. Translation: BAA22041.1.
    U60330 mRNA. Translation: AAC53297.1.
    AB007139 Genomic DNA. Translation: BAA28838.1.
    BC015255 mRNA. Translation: AAH15255.1.
    CCDSiCCDS25463.1.
    RefSeqiNP_035322.1. NM_011192.3.
    UniGeneiMm.288477.

    Genome annotation databases

    EnsembliENSMUST00000019470; ENSMUSP00000019470; ENSMUSG00000078652.
    GeneIDi19192.
    KEGGimmu:19192.
    UCSCiuc007lon.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D87911 mRNA. Translation: BAA22041.1 .
    U60330 mRNA. Translation: AAC53297.1 .
    AB007139 Genomic DNA. Translation: BAA28838.1 .
    BC015255 mRNA. Translation: AAH15255.1 .
    CCDSi CCDS25463.1.
    RefSeqi NP_035322.1. NM_011192.3.
    UniGenei Mm.288477.

    3D structure databases

    ProteinModelPortali P61290.
    SMRi P61290. Positions 5-64, 112-246.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202435. 5 interactions.
    IntActi P61290. 1 interaction.

    PTM databases

    PhosphoSitei P61290.

    2D gel databases

    REPRODUCTION-2DPAGE P61290.

    Proteomic databases

    MaxQBi P61290.
    PaxDbi P61290.
    PRIDEi P61290.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000019470 ; ENSMUSP00000019470 ; ENSMUSG00000078652 .
    GeneIDi 19192.
    KEGGi mmu:19192.
    UCSCi uc007lon.1. mouse.

    Organism-specific databases

    CTDi 10197.
    MGIi MGI:1096366. Psme3.

    Phylogenomic databases

    eggNOGi NOG236353.
    HOGENOMi HOG000282822.
    HOVERGENi HBG053745.
    KOi K06698.
    OMAi FVMPGGM.
    PhylomeDBi P61290.
    TreeFami TF106236.

    Enzyme and pathway databases

    Reactomei REACT_198693. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_199105. ER-Phagosome pathway.
    REACT_199114. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_199115. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_199121. Activation of NF-kappaB in B cells.
    REACT_203517. SCF-beta-TrCP mediated degradation of Emi1.
    REACT_203973. Asymmetric localization of PCP proteins.
    REACT_207679. Separation of Sister Chromatids.
    REACT_212633. Autodegradation of the E3 ubiquitin ligase COP1.
    REACT_218318. Regulation of activated PAK-2p34 by proteasome mediated degradation.
    REACT_219129. degradation of AXIN.
    REACT_219897. APC/C:Cdc20 mediated degradation of Securin.
    REACT_220647. SCF(Skp2)-mediated degradation of p27/p21.
    REACT_225686. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_226135. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
    REACT_227429. degradation of DVL.

    Miscellaneous databases

    NextBioi 295900.
    PROi P61290.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P61290.
    Bgeei P61290.
    CleanExi MM_PSME3.
    Genevestigatori P61290.

    Family and domain databases

    Gene3Di 1.20.120.180. 1 hit.
    1.20.5.120. 1 hit.
    InterProi IPR009077. Proteasome_activ_pa28.
    IPR003186. Proteasome_activ_pa28_C.
    IPR003185. Proteasome_activ_pa28_N.
    [Graphical view ]
    PANTHERi PTHR10660. PTHR10660. 1 hit.
    Pfami PF02251. PA28_alpha. 1 hit.
    PF02252. PA28_beta. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47216. SSF47216. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "PA28 subunits of the mouse proteasome: primary structures and chromosomal localization of the genes."
      Kandil E., Kohda K., Ishibashi T., Tanaka K., Kasahara M.
      Immunogenetics 46:337-344(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Spleen.
    2. "Sequence and expression of mouse proteasome activator PA28 and the related autoantigen Ki."
      Jiang H., Monaco J.J.
      Immunogenetics 46:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: B10.BR.
    3. "Characterization of the mouse PA28 activator complex gene family: complete organizations of the three member genes and a physical map of the approximately 150-kb region containing the alpha- and beta-subunit genes."
      Kohda K., Ishibashi T., Shimbara N., Tanaka K., Matsuda Y., Kasahara M.
      J. Immunol. 160:4923-4935(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/SvJ.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Kidney.
    5. "Growth retardation in mice lacking the proteasome activator PA28gamma."
      Murata S., Kawahara H., Tohma S., Yamamoto K., Kasahara M., Nabeshima Y., Tanaka K., Chiba T.
      J. Biol. Chem. 274:38211-38215(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    6. "MEKK3 interacts with the PA28 gamma regulatory subunit of the proteasome."
      Hagemann C., Patel R., Blank J.L.
      Biochem. J. 373:71-79(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MAP3K3, PHOSPHORYLATION BY MAP3K3, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiPSME3_MOUSE
    AccessioniPrimary (citable) accession number: P61290
    Secondary accession number(s): O35563
    , P97373, Q12920, Q13172, Q9BQD9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2004
    Last sequence update: May 10, 2004
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3