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P61290 (PSME3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proteasome activator complex subunit 3
Alternative name(s):
11S regulator complex subunit gamma
Short name=REG-gamma
Activator of multicatalytic protease subunit 3
Ki nuclear autoantigen
Proteasome activator 28 subunit gamma
Short name=PA28g
Short name=PA28gamma
Gene names
Name:Psme3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length254 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation By similarity.

Subunit structure

Homoheptamer; the stability of the heptamer is essential for the specific activation of the trypsine-like subunit and inhibition of the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits of the proteasome By similarity. Interacts with p53/TP53 and MDM2 By similarity. Interacts with MAP3K3. Associates with the proteasome By similarity. Ref.6

Subcellular location

Nucleus. Cytoplasm. Note: Localizes to the cytoplasm during mitosis following nuclear envelope breakdown at this distinct stage of the cell cycle which allows its interaction with MAP3K3 kinase. Ref.6

Domain

The C-terminal sequences affect heptamer stability and proteasome affinity By similarity.

Post-translational modification

Phosphorylated by MAP3K3. Ref.6

Acetylation at the major site Lys-195 is important for oligomerization and ability to degrade its target substrates. Deacetylated by SIRT1 By similarity.

Disruption phenotype

Growth retardation, small body size, and reduces significantly the percentage of cells that enter the S phase. Ref.5

Sequence similarities

Belongs to the PA28 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 254253Proteasome activator complex subunit 3
PRO_0000161790

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue61N6-acetyllysine By similarity
Modified residue141N6-acetyllysine By similarity
Modified residue241Phosphoserine By similarity
Modified residue1951N6-acetyllysine; by P300/CBP By similarity

Experimental info

Sequence conflict21A → L in AAC53297. Ref.2
Sequence conflict91Q → L in AAC53297. Ref.2
Sequence conflict231T → S in AAC53297. Ref.2
Sequence conflict1171D → G in AAC53297. Ref.2
Sequence conflict1721A → P in AAC53297. Ref.2
Sequence conflict1881K → G in AAC53297. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P61290 [UniParc].

Last modified May 10, 2004. Version 1.
Checksum: 116FAB47D60A26C0

FASTA25429,506
        10         20         30         40         50         60 
MASLLKVDQE VKLKVDSFRE RITSEAEDLV ANFFPKKLLE LDSFLKEPIL NIHDLTQIHS 

        70         80         90        100        110        120 
DMNLPVPDPI LLTNSHDGLD GPTYKKRRLD ECEEAFQGTK VFVMPNGMLK SNQQLVDIIE 

       130        140        150        160        170        180 
KVKPEIRLLI EKCNTVKMWV QLLIPRIEDG NNFGVSIQEE TVAELRTVES EAASYLDQIS 

       190        200        210        220        230        240 
RYYITRAKLV SKIAKYPHVE DYRRTVTEID EKEYISLRLI ISELRNQYVT LHDMILKNIE 

       250 
KIKRPRSSNA ETLY 

« Hide

References

« Hide 'large scale' references
[1]"PA28 subunits of the mouse proteasome: primary structures and chromosomal localization of the genes."
Kandil E., Kohda K., Ishibashi T., Tanaka K., Kasahara M.
Immunogenetics 46:337-344(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
Tissue: Spleen.
[2]"Sequence and expression of mouse proteasome activator PA28 and the related autoantigen Ki."
Jiang H., Monaco J.J.
Immunogenetics 46:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: B10.BR.
[3]"Characterization of the mouse PA28 activator complex gene family: complete organizations of the three member genes and a physical map of the approximately 150-kb region containing the alpha- and beta-subunit genes."
Kohda K., Ishibashi T., Shimbara N., Tanaka K., Matsuda Y., Kasahara M.
J. Immunol. 160:4923-4935(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/SvJ.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Kidney.
[5]"Growth retardation in mice lacking the proteasome activator PA28gamma."
Murata S., Kawahara H., Tohma S., Yamamoto K., Kasahara M., Nabeshima Y., Tanaka K., Chiba T.
J. Biol. Chem. 274:38211-38215(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
[6]"MEKK3 interacts with the PA28 gamma regulatory subunit of the proteasome."
Hagemann C., Patel R., Blank J.L.
Biochem. J. 373:71-79(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MAP3K3, PHOSPHORYLATION BY MAP3K3, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D87911 mRNA. Translation: BAA22041.1.
U60330 mRNA. Translation: AAC53297.1.
AB007139 Genomic DNA. Translation: BAA28838.1.
BC015255 mRNA. Translation: AAH15255.1.
RefSeqNP_035322.1. NM_011192.3.
UniGeneMm.288477.

3D structure databases

ProteinModelPortalP61290.
SMRP61290. Positions 5-64, 112-246.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202435. 5 interactions.

PTM databases

PhosphoSiteP61290.

2D gel databases

REPRODUCTION-2DPAGEP61290.

Proteomic databases

PaxDbP61290.
PRIDEP61290.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000019470; ENSMUSP00000019470; ENSMUSG00000078652.
GeneID19192.
KEGGmmu:19192.
UCSCuc007lon.1. mouse.

Organism-specific databases

CTD10197.
MGIMGI:1096366. Psme3.

Phylogenomic databases

eggNOGNOG236353.
HOGENOMHOG000282822.
HOVERGENHBG053745.
KOK06698.
OMALKMWISF.
PhylomeDBP61290.
TreeFamTF106236.

Gene expression databases

ArrayExpressP61290.
BgeeP61290.
CleanExMM_PSME3.
GenevestigatorP61290.

Family and domain databases

Gene3D1.20.120.180. 1 hit.
1.20.5.120. 1 hit.
InterProIPR009077. Proteasome_activ_pa28.
IPR003186. Proteasome_activ_pa28_C.
IPR003185. Proteasome_activ_pa28_N.
[Graphical view]
PANTHERPTHR10660. PTHR10660. 1 hit.
PfamPF02251. PA28_alpha. 1 hit.
PF02252. PA28_beta. 1 hit.
[Graphical view]
SUPFAMSSF47216. SSF47216. 2 hits.
ProtoNetSearch...

Other

NextBio295900.
PROP61290.
SOURCESearch...

Entry information

Entry namePSME3_MOUSE
AccessionPrimary (citable) accession number: P61290
Secondary accession number(s): O35563 expand/collapse secondary AC list , P97373, Q12920, Q13172, Q9BQD9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: April 16, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot