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Protein

Proteasome activator complex subunit 3

Gene

Psme3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. Mediates CCAR2 and CHEK2-dependent SIRT1 inhibition (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis, Cell cycle

Enzyme and pathway databases

ReactomeiR-MMU-1169091. Activation of NF-kappaB in B cells.
R-MMU-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-MMU-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-MMU-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-MMU-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-MMU-174154. APC/C:Cdc20 mediated degradation of Securin.
R-MMU-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-MMU-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-MMU-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-MMU-195253. Degradation of beta-catenin by the destruction complex.
R-MMU-202424. Downstream TCR signaling.
R-MMU-2467813. Separation of Sister Chromatids.
R-MMU-2871837. FCERI mediated NF-kB activation.
R-MMU-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-MMU-350562. Regulation of ornithine decarboxylase (ODC).
R-MMU-382556. ABC-family proteins mediated transport.
R-MMU-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-MMU-4608870. Asymmetric localization of PCP proteins.
R-MMU-4641257. Degradation of AXIN.
R-MMU-4641258. Degradation of DVL.
R-MMU-5358346. Hedgehog ligand biogenesis.
R-MMU-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-MMU-5607764. CLEC7A (Dectin-1) signaling.
R-MMU-5610780. Degradation of GLI1 by the proteasome.
R-MMU-5610785. GLI3 is processed to GLI3R by the proteasome.
R-MMU-5632684. Hedgehog 'on' state.
R-MMU-5658442. Regulation of RAS by GAPs.
R-MMU-5668541. TNFR2 non-canonical NF-kB pathway.
R-MMU-5676590. NIK-->noncanonical NF-kB signaling.
R-MMU-5687128. MAPK6/MAPK4 signaling.
R-MMU-5689603. UCH proteinases.
R-MMU-5689880. Ub-specific processing proteases.
R-MMU-68827. CDT1 association with the CDC6:ORC:origin complex.
R-MMU-68949. Orc1 removal from chromatin.
R-MMU-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-MMU-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-MMU-69481. G2/M Checkpoints.
R-MMU-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-MMU-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-MMU-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome activator complex subunit 3
Alternative name(s):
11S regulator complex subunit gamma
Short name:
REG-gamma
Activator of multicatalytic protease subunit 3
Ki nuclear autoantigen
Proteasome activator 28 subunit gamma
Short name:
PA28g
Short name:
PA28gamma
Gene namesi
Name:Psme3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1096366. Psme3.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm 1 Publication

  • Note: Localizes to the cytoplasm during mitosis following nuclear envelope breakdown at this distinct stage of the cell cycle which allows its interaction with MAP3K3 kinase.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

Pathology & Biotechi

Disruption phenotypei

Growth retardation, small body size, and reduces significantly the percentage of cells that enter the S phase.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001617902 – 254Proteasome activator complex subunit 3Add BLAST253

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei17PhosphoserineBy similarity1
Modified residuei24PhosphoserineBy similarity1
Modified residuei195N6-acetyllysine; by P300/CBPBy similarity1
Modified residuei247Phosphoserine; by CHEK2By similarity1

Post-translational modificationi

Phosphorylated by MAP3K3. Phosphorylation at Ser-247 promotes its association with CCAR2 (By similarity).By similarity1 Publication
Acetylation at the major site Lys-195 is important for oligomerization and ability to degrade its target substrates. Deacetylated by SIRT1 (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP61290.
PaxDbiP61290.
PeptideAtlasiP61290.
PRIDEiP61290.
TopDownProteomicsiP61290.

2D gel databases

REPRODUCTION-2DPAGEP61290.

PTM databases

iPTMnetiP61290.
PhosphoSitePlusiP61290.

Expressioni

Gene expression databases

BgeeiENSMUSG00000078652.
CleanExiMM_PSME3.
ExpressionAtlasiP61290. baseline and differential.
GenevisibleiP61290. MM.

Interactioni

Subunit structurei

Homoheptamer; the stability of the heptamer is essential for the specific activation of the trypsine-like subunit and inhibition of the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits of the proteasome (By similarity). Interacts with p53/TP53 and MDM2 (By similarity). Interacts with MAP3K3 (PubMed:12650640). Associates with the proteasome (By similarity). Interacts with CCAR2 (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202435. 7 interactors.
IntActiP61290. 2 interactors.
STRINGi10090.ENSMUSP00000019470.

Structurei

3D structure databases

ProteinModelPortaliP61290.
SMRiP61290.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The C-terminal sequences affect heptamer stability and proteasome affinity.By similarity

Sequence similaritiesi

Belongs to the PA28 family.Curated

Phylogenomic databases

eggNOGiKOG4470. Eukaryota.
ENOG410XQVX. LUCA.
GeneTreeiENSGT00510000046374.
HOGENOMiHOG000282822.
HOVERGENiHBG053745.
InParanoidiP61290.
KOiK06698.
OMAiPCNKPLC.
OrthoDBiEOG091G0GX7.
PhylomeDBiP61290.
TreeFamiTF106236.

Family and domain databases

Gene3Di1.20.120.180. 1 hit.
1.20.5.120. 1 hit.
InterProiIPR009077. Proteasome_activ_pa28.
IPR003186. Proteasome_activ_pa28_C.
IPR003185. Proteasome_activ_pa28_N.
[Graphical view]
PANTHERiPTHR10660. PTHR10660. 1 hit.
PfamiPF02251. PA28_alpha. 1 hit.
PF02252. PA28_beta. 1 hit.
[Graphical view]
SUPFAMiSSF47216. SSF47216. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61290-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASLLKVDQE VKLKVDSFRE RITSEAEDLV ANFFPKKLLE LDSFLKEPIL
60 70 80 90 100
NIHDLTQIHS DMNLPVPDPI LLTNSHDGLD GPTYKKRRLD ECEEAFQGTK
110 120 130 140 150
VFVMPNGMLK SNQQLVDIIE KVKPEIRLLI EKCNTVKMWV QLLIPRIEDG
160 170 180 190 200
NNFGVSIQEE TVAELRTVES EAASYLDQIS RYYITRAKLV SKIAKYPHVE
210 220 230 240 250
DYRRTVTEID EKEYISLRLI ISELRNQYVT LHDMILKNIE KIKRPRSSNA

ETLY
Length:254
Mass (Da):29,506
Last modified:May 10, 2004 - v1
Checksum:i116FAB47D60A26C0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2A → L in AAC53297 (PubMed:9162094).Curated1
Sequence conflicti9Q → L in AAC53297 (PubMed:9162094).Curated1
Sequence conflicti23T → S in AAC53297 (PubMed:9162094).Curated1
Sequence conflicti117D → G in AAC53297 (PubMed:9162094).Curated1
Sequence conflicti172A → P in AAC53297 (PubMed:9162094).Curated1
Sequence conflicti188K → G in AAC53297 (PubMed:9162094).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87911 mRNA. Translation: BAA22041.1.
U60330 mRNA. Translation: AAC53297.1.
AB007139 Genomic DNA. Translation: BAA28838.1.
BC015255 mRNA. Translation: AAH15255.1.
CCDSiCCDS25463.1.
RefSeqiNP_035322.1. NM_011192.3.
UniGeneiMm.288477.

Genome annotation databases

EnsembliENSMUST00000019470; ENSMUSP00000019470; ENSMUSG00000078652.
GeneIDi19192.
KEGGimmu:19192.
UCSCiuc007lon.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87911 mRNA. Translation: BAA22041.1.
U60330 mRNA. Translation: AAC53297.1.
AB007139 Genomic DNA. Translation: BAA28838.1.
BC015255 mRNA. Translation: AAH15255.1.
CCDSiCCDS25463.1.
RefSeqiNP_035322.1. NM_011192.3.
UniGeneiMm.288477.

3D structure databases

ProteinModelPortaliP61290.
SMRiP61290.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202435. 7 interactors.
IntActiP61290. 2 interactors.
STRINGi10090.ENSMUSP00000019470.

PTM databases

iPTMnetiP61290.
PhosphoSitePlusiP61290.

2D gel databases

REPRODUCTION-2DPAGEP61290.

Proteomic databases

EPDiP61290.
PaxDbiP61290.
PeptideAtlasiP61290.
PRIDEiP61290.
TopDownProteomicsiP61290.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000019470; ENSMUSP00000019470; ENSMUSG00000078652.
GeneIDi19192.
KEGGimmu:19192.
UCSCiuc007lon.1. mouse.

Organism-specific databases

CTDi10197.
MGIiMGI:1096366. Psme3.

Phylogenomic databases

eggNOGiKOG4470. Eukaryota.
ENOG410XQVX. LUCA.
GeneTreeiENSGT00510000046374.
HOGENOMiHOG000282822.
HOVERGENiHBG053745.
InParanoidiP61290.
KOiK06698.
OMAiPCNKPLC.
OrthoDBiEOG091G0GX7.
PhylomeDBiP61290.
TreeFamiTF106236.

Enzyme and pathway databases

ReactomeiR-MMU-1169091. Activation of NF-kappaB in B cells.
R-MMU-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-MMU-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-MMU-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-MMU-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-MMU-174154. APC/C:Cdc20 mediated degradation of Securin.
R-MMU-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-MMU-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-MMU-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-MMU-195253. Degradation of beta-catenin by the destruction complex.
R-MMU-202424. Downstream TCR signaling.
R-MMU-2467813. Separation of Sister Chromatids.
R-MMU-2871837. FCERI mediated NF-kB activation.
R-MMU-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-MMU-350562. Regulation of ornithine decarboxylase (ODC).
R-MMU-382556. ABC-family proteins mediated transport.
R-MMU-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-MMU-4608870. Asymmetric localization of PCP proteins.
R-MMU-4641257. Degradation of AXIN.
R-MMU-4641258. Degradation of DVL.
R-MMU-5358346. Hedgehog ligand biogenesis.
R-MMU-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-MMU-5607764. CLEC7A (Dectin-1) signaling.
R-MMU-5610780. Degradation of GLI1 by the proteasome.
R-MMU-5610785. GLI3 is processed to GLI3R by the proteasome.
R-MMU-5632684. Hedgehog 'on' state.
R-MMU-5658442. Regulation of RAS by GAPs.
R-MMU-5668541. TNFR2 non-canonical NF-kB pathway.
R-MMU-5676590. NIK-->noncanonical NF-kB signaling.
R-MMU-5687128. MAPK6/MAPK4 signaling.
R-MMU-5689603. UCH proteinases.
R-MMU-5689880. Ub-specific processing proteases.
R-MMU-68827. CDT1 association with the CDC6:ORC:origin complex.
R-MMU-68949. Orc1 removal from chromatin.
R-MMU-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-MMU-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-MMU-69481. G2/M Checkpoints.
R-MMU-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-MMU-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-MMU-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

ChiTaRSiPsme3. mouse.
PROiP61290.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000078652.
CleanExiMM_PSME3.
ExpressionAtlasiP61290. baseline and differential.
GenevisibleiP61290. MM.

Family and domain databases

Gene3Di1.20.120.180. 1 hit.
1.20.5.120. 1 hit.
InterProiIPR009077. Proteasome_activ_pa28.
IPR003186. Proteasome_activ_pa28_C.
IPR003185. Proteasome_activ_pa28_N.
[Graphical view]
PANTHERiPTHR10660. PTHR10660. 1 hit.
PfamiPF02251. PA28_alpha. 1 hit.
PF02252. PA28_beta. 1 hit.
[Graphical view]
SUPFAMiSSF47216. SSF47216. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiPSME3_MOUSE
AccessioniPrimary (citable) accession number: P61290
Secondary accession number(s): O35563
, P97373, Q12920, Q13172, Q9BQD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: November 30, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.