Proteasome activator complex subunit 3

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P61290 (PSME3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 72. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Proteasome activator complex subunit 3

Alternative name(s):
11S regulator complex subunit gamma
Short name=REG-gamma
Activator of multicatalytic protease subunit 3
Ki nuclear autoantigen
Proteasome activator 28 subunit gamma
Short name=PA28g
Short name=PA28gamma

Gene names

Name:

Psme3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation By similarity.
Subunit structure	Homoheptamer; the stability of the heptamer is essential for the specific activation of the trypsine-like subunit and inhibition of the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits of the proteasome By similarity. Interacts with p53/TP53 and MDM2 By similarity. Interacts with MAP3K3. Associates with the proteasome By similarity. Ref.6
Subcellular location	Nucleus. Cytoplasm. Note: Localizes to the cytoplasm during mitosis following nuclear envelope breakdown at this distinct stage of the cell cycle which allows its interaction with MAP3K3 kinase. Ref.6
Domain	The C-terminal sequences affect heptamer stability and proteasome affinity By similarity.
Post-translational modification	Phosphorylated by MAP3K3. Ref.6
Disruption phenotype	Growth retardation, small body size, and reduces significantly the percentage of cells that enter the S phase. Ref.5
Sequence similarities	Belongs to the PA28 family.

Ontologies

Keywords
Biological process	Apoptosis Cell cycle
Cellular component	Cytoplasm Nucleus Proteasome
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW regulation of apoptotic process Inferred from sequence or structural similarity. Source: UniProtKB regulation of proteasomal protein catabolic process Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell proteasome activator complex Inferred from electronic annotation. Source: InterPro
Molecular_function	MDM2/MDM4 family protein binding Inferred from sequence or structural similarity. Source: UniProtKB endopeptidase activator activity Inferred from electronic annotation. Source: Compara p53 binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed By similarity

Chain

2 – 254

253

Proteasome activator complex subunit 3

PRO_0000161790

Amino acid modifications

Modified residue

N-acetylalanine By similarity

Modified residue

Phosphoserine By similarity

Modified residue

205

Phosphothreonine Ref.7

Modified residue

207

Phosphothreonine Ref.7

Experimental info

Sequence conflict

A → L in AAC53297. Ref.2

Sequence conflict

Q → L in AAC53297. Ref.2

Sequence conflict

T → S in AAC53297. Ref.2

Sequence conflict

117

D → G in AAC53297. Ref.2

Sequence conflict

172

A → P in AAC53297. Ref.2

Sequence conflict

188

K → G in AAC53297. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P61290 [UniParc].

Last modified May 10, 2004. Version 1.
Checksum: 116FAB47D60A26C0
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FASTA

254

29,506

        10         20         30         40         50         60 
MASLLKVDQE VKLKVDSFRE RITSEAEDLV ANFFPKKLLE LDSFLKEPIL NIHDLTQIHS 

        70         80         90        100        110        120 
DMNLPVPDPI LLTNSHDGLD GPTYKKRRLD ECEEAFQGTK VFVMPNGMLK SNQQLVDIIE 

       130        140        150        160        170        180 
KVKPEIRLLI EKCNTVKMWV QLLIPRIEDG NNFGVSIQEE TVAELRTVES EAASYLDQIS 

       190        200        210        220        230        240 
RYYITRAKLV SKIAKYPHVE DYRRTVTEID EKEYISLRLI ISELRNQYVT LHDMILKNIE 

       250 
KIKRPRSSNA ETLY

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PA28 subunits of the mouse proteasome: primary structures and chromosomal localization of the genes." Kandil E., Kohda K., Ishibashi T., Tanaka K., Kasahara M. Immunogenetics 46:337-344(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Spleen.
[2]	"Sequence and expression of mouse proteasome activator PA28 and the related autoantigen Ki." Jiang H., Monaco J.J. Immunogenetics 46:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: B10.BR.
[3]	"Characterization of the mouse PA28 activator complex gene family: complete organizations of the three member genes and a physical map of the approximately 150-kb region containing the alpha- and beta-subunit genes." Kohda K., Ishibashi T., Shimbara N., Tanaka K., Matsuda Y., Kasahara M. J. Immunol. 160:4923-4935(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/SvJ.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Kidney.
[5]	"Growth retardation in mice lacking the proteasome activator PA28gamma." Murata S., Kawahara H., Tohma S., Yamamoto K., Kasahara M., Nabeshima Y., Tanaka K., Chiba T. J. Biol. Chem. 274:38211-38215(1999) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE.
[6]	"MEKK3 interacts with the PA28 gamma regulatory subunit of the proteasome." Hagemann C., Patel R., Blank J.L. Biochem. J. 373:71-79(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MAP3K3, PHOSPHORYLATION BY MAP3K3, SUBCELLULAR LOCATION.
[7]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-205 AND THR-207, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D87911 mRNA. Translation: BAA22041.1. U60330 mRNA. Translation: AAC53297.1. AB007139 Genomic DNA. Translation: BAA28838.1. BC015255 mRNA. Translation: AAH15255.1.
IPI	IPI00113660.
RefSeq	NP_035322.1. NM_011192.3.
UniGene	Mm.288477.
3D structure databases
ProteinModelPortal	P61290.
ModBase	Search...
PTM databases
PhosphoSite	P61290.
2D gel databases
REPRODUCTION-2DPAGE	P61290.
Proteomic databases
PaxDb	P61290.
PRIDE	P61290.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000019470; ENSMUSP00000019470; ENSMUSG00000078652.
GeneID	19192.
KEGG	mmu:19192.
Organism-specific databases
CTD	10197.
MGI	MGI:1096366. Psme3.
Phylogenomic databases
eggNOG	NOG236353.
HOGENOM	HOG000282822.
HOVERGEN	HBG053745.
KO	K06698.
OMA	LKMWISF.
OrthoDB	EOG4NS3C9.
Gene expression databases
ArrayExpress	P61290.
Bgee	P61290.
CleanEx	MM_PSME3.
Genevestigator	P61290.
GermOnline	ENSMUSG00000019326. Mus musculus.
Family and domain databases
Gene3D	1.20.120.180. 1 hit. 1.20.5.120. 1 hit.
InterPro	IPR003185. Proteasome_activ_REG_asu. IPR009077. Proteasome_activ_REG_asu/bsu. IPR003186. Proteasome_activ_REG_bsu. [Graphical view]
PANTHER	PTHR10660. PTHR10660. 1 hit.
Pfam	PF02251. PA28_alpha. 1 hit. PF02252. PA28_beta. 1 hit. [Graphical view]
SUPFAM	SSF47216. Prot_act_regA. 1 hit.
ProtoNet	Search...
Other
NextBio	295900.
SOURCE	Search...

Entry information

Entry name

PSME3_MOUSE

Accession

Primary (citable) accession number: P61290
Secondary accession number(s): O35563

Q9BQD9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	May 10, 2004
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents