P61277 (CATK_MACMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cathepsin K
EC=3.4.22.38

Gene names

Name:

CTSK

Organism

Macaca mulatta (Rhesus macaque)

Taxonomic identifier

9544 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation By similarity.
Catalytic activity	Broad proteolytic activity. With small-molecule substrates and inhibitors, the major determinant of specificity is P2, which is preferably Leu, Met > Phe, and not Arg.
Subcellular location	Lysosome By similarity.
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 15

15

Potential

Propeptide

16 – 114

99

Activation peptide

PRO_0000026299

Chain

115 – 329

215

Cathepsin K

PRO_0000026300

Sites

Active site

139

1

By similarity

Active site

276

1

By similarity

Active site

296

1

By similarity

Amino acid modifications

Glycosylation

103

1

N-linked (GlcNAc...) Potential

Disulfide bond

136 ↔ 177

By similarity

Disulfide bond

170 ↔ 210

By similarity

Disulfide bond

269 ↔ 318

By similarity

Secondary structure

1

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329

Helix Strand Turn

Details...

Helix

121 – 124

4

Beta strand

135 – 137

3

Helix

139 – 156

18

Helix

164 – 170

7

Helix

176 – 178

3

Helix

182 – 192

11

Beta strand

195 – 197

3

Turn

198 – 200

3

Helix

214 – 216

3

Beta strand

217 – 219

3

Beta strand

222 – 226

5

Helix

232 – 241

10

Beta strand

245 – 249

5

Helix

254 – 257

4

Beta strand

261 – 264

4

Beta strand

276 – 288

13

Beta strand

290 – 295

6

Beta strand

307 – 311

5

Beta strand

313 – 316

4

Helix

317 – 319

3

Beta strand

325 – 328

4

Sequences

	Sequence		Length	Mass (Da)
	P61277 [UniParc]. Last modified May 10, 2004. Version 1. Checksum: 652DD8DFC37FB984	FASTA	329	36,942
	10 20 30 40 50 60 MWGLKVLLLP VMSFALYPEE ILDTHWELWK KTHRKQYNSK VDEISRRLIW EKNLKYISIH 70 80 90 100 110 120 NLEASLGVHT YELAMNHLGD MTNEEVVQKM TGLKVPASHS RSNDTLYIPD WEGRAPDSVD 130 140 150 160 170 180 YRKKGYVTPV KNQGQCGSCW AFSSVGALEG QLKKKTGKLL NLSPQNLVDC VSENDGCGGG 190 200 210 220 230 240 YMTNAFQYVQ KNRGIDSEDA YPYVGQEESC MYNPTGKAAK CRGYREIPEG NEKALKRAVA 250 260 270 280 290 300 RVGPVSVAID ASLTSFQFYS KGVYYDESCN SDNLNHAVLA VGYGIQKGNK HWIIKNSWGE 310 320 NWGNKGYILM ARNKNNACGI ANLASFPKM

References

[1]	"Cloning and expression of rhesus monkey cathepsin K." Guay J., Riendeau D., Mancini J.A. Bone 25:205-209(1999) [PubMed: 10456386] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF124092 mRNA. Translation: AAD33249.1.

RefSeq

NP_001027984.1. NM_001032812.1.

UniGene

Mmu.3465.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2FTD	X-ray	2.55	A/B	115-329	[»]

ProteinModelPortal

P61277.

SMR

P61277. Positions 16-329.

ModBase

Search...

Protein-protein interaction databases

STRING

P61277.

Protein family/group databases

MEROPS

C01.036.

Proteomic databases

PRIDE

P61277.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMMUT00000000786; ENSMMUP00000000728; ENSMMUG00000000537.

GeneID

574112.

KEGG

mcc:574112.

Organism-specific databases

CTD

1513.

Phylogenomic databases

eggNOG

prNOG05230.

GeneTree

ENSGT00560000076577.

HOVERGEN

HBG011513.

InParanoid

P61277.

OMA

LKVPPSH.

PhylomeDB

P61277.

Family and domain databases

InterPro

IPR000169. Pept_cys_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR015644. Peptidase_C1A_cathepsin-K.
IPR013201. Prot_inhib_I29.
[Graphical view]

KO

K01371.

PANTHER

PTHR12411:SF55. CathpsnK_like. 1 hit.
PTHR12411. Peptidase_C1A. 1 hit.

Pfam

PF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]

PRINTS

PR00705. PAPAIN.

SMART

SM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]

PROSITE

PS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CATK_MACMU

Accession

Primary (citable) accession number: P61277
Secondary accession number(s): O77641

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	May 10, 2004
Last modified:	November 16, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program