P61265 (STX1B_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Syntaxin-1B

Alternative name(s):
P35B
Syntaxin-1B2

Gene names

Name:	Stx1b
Synonyms:	Stx1b2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Potentially involved in docking of synaptic vesicles at presynaptic active zones. May mediate Ca2+-regulation of exocytosis acrosomal reaction in sperm By similarity.
Subunit structure	Interacts with OTOF. Interacts with SYT6 and SYT8; the interaction is Ca2+-dependent By similarity.
Subcellular location	Membrane; Single-pass type IV membrane protein Potential.
Tissue specificity	Cerebral cortex, hippocampus, cerebellum, and adrenal medulla.
Post-translational modification	Phosphorylated by CK2. Ref.4
Sequence similarities	Belongs to the syntaxin family. Contains 1 t-SNARE coiled-coil homology domain.

Ontologies

Keywords
Biological process	Neurotransmitter transport Transport
Cellular component	Membrane
Domain	Coiled coil Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	intracellular protein transport Inferred from electronic annotation. Source: InterPro neurotransmitter transport Inferred from electronic annotation. Source: UniProtKB-KW protein transport Inferred from mutant phenotype PubMed 11245593. Source: RGD regulation of exocytosis Inferred from mutant phenotype PubMed 11245593. Source: RGD synaptic transmission Inferred from electronic annotation. Source: InterPro vesicle-mediated transport Inferred from mutant phenotype PubMed 11245593. Source: RGD
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Rnf40	Q8CJB9	4	EBI-2255905,EBI-6110162

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	Syntaxin-1B		PRO_0000210194
Regions
	Topological domain	1 – 264	264	Cytoplasmic Potential
	Transmembrane	265 – 288	24	Helical; Anchor for type IV membrane protein; Potential
	Domain	191 – 253	63	t-SNARE coiled-coil homology
	Coiled coil	29 – 104	76	Potential
Amino acid modifications
	Modified residue	14	1	Phosphoserine Ref.4
	Modified residue	58	1	Phosphoserine By similarity
	Modified residue	160	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P61265 [UniParc]. Last modified May 10, 2004. Version 1. Checksum: 2733497E02978BEB	FASTA	288	33,245
	10 20 30 40 50 60 MKDRTQELRS AKDSDDEEEV VHVDRDHFMD EFFEQVEEIR GCIEKLSEDV EQVKKQHSAI 70 80 90 100 110 120 LAAPNPDEKT KQELEDLTAD IKKTANKVRS KLKAIEQSIE QEEGLNRSSA DLRIRKTQHS 130 140 150 160 170 180 TLSRKFVEVM TEYNATQSKY RDRCKDRIQR QLEITGRTTT NEELEDMLES GKLAIFTDDI 190 200 210 220 230 240 KMDSQMTKQA LNEIETRHNE IIKLETSIRE LHDMFVDMAM LVESQGEMID RIEYNVEHSV 250 260 270 280 DYVERAVSDT KKAVKYQSKA RRKKIMIIIC CVVLGVVLAS SIGGTLGL

References

[1]	"Syntaxin: a synaptic protein implicated in docking of synaptic vesicles at presynaptic active zones." Bennett M.K., Calakos N., Scheller R.H. Science 257:255-259(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-16 AND 31-55. Tissue: Brain.
[2]	"The syntaxin family of vesicular transport receptors." Bennett M.K., Garcia-Arraras J.E., Elferink L.A., Peterson K.E., Fleming A.M., Hazuka C.D., Scheller R.H. Cell 74:863-873(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	Lubec G., Kang S.U., Lubec S. Submitted (SEP-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 189-197; 204-209 AND 232-245, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain.
[4]	"A method for the comprehensive proteomic analysis of membrane proteins." Wu C.C., MacCoss M.J., Howell K.E., Yates J.R. III Nat. Biotechnol. 21:532-538(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-14.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M95735 mRNA. Translation: AAA42197.1.
IPI	IPI00191730.
PIR	B48213.
RefSeq	NP_036832.1. NM_012700.2.
UniGene	Rn.98793.
3D structure databases
ProteinModelPortal	P61265.
ModBase	Search...
Protein-protein interaction databases
IntAct	P61265. 1 interaction.
PTM databases
PhosphoSite	P61265.
Proteomic databases
PaxDb	P61265.
PRIDE	P61265.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000026063; ENSRNOP00000026063; ENSRNOG00000019193.
GeneID	24923.
KEGG	rno:24923.
UCSC	RGD:3784. rat.
Organism-specific databases
CTD	112755.
RGD	3784. Stx1b.
Phylogenomic databases
eggNOG	COG5074.
GeneTree	ENSGT00550000074334.
HOGENOM	HOG000286023.
HOVERGEN	HBG000497.
InParanoid	P61265.
KO	K08486.
OMA	HEETKAV.
OrthoDB	EOG4PC9SV.
Gene expression databases
Genevestigator	P61265.
GermOnline	ENSRNOG00000019193. Rattus norvegicus.
Family and domain databases
InterPro	IPR015709. Syntaxin-1. IPR006012. Syntaxin/epimorphin_CS. IPR006011. Syntaxin_N. IPR010989. t-SNARE. IPR000727. T_SNARE_dom. [Graphical view]
PANTHER	PTHR19957:SF35. PTHR19957:SF35. 1 hit.
Pfam	PF05739. SNARE. 1 hit. PF00804. Syntaxin. 1 hit. [Graphical view]
SMART	SM00503. SynN. 1 hit. SM00397. t_SNARE. 1 hit. [Graphical view]
SUPFAM	SSF47661. t-snare. 1 hit.
PROSITE	PS00914. SYNTAXIN. 1 hit. PS50192. T_SNARE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	604871.

Entry information

Entry name

STX1B_RAT

Accession

Primary (citable) accession number: P61265
Secondary accession number(s): P32853

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	May 10, 2004
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program