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Protein

Kappa-theraphotoxin-Ps1a

Gene
N/A
Organism
Paraphysa scrofa (Chilean copper tarantula) (Phrixotrichus auratus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Potently blocks A-type potassium currents. Specifically blocks Kv4.2/KCND2 and Kv4.3/KCND3 channels by altering the gating properties of these channels.1 Publication

Keywords - Molecular functioni

Ion channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Toxin, Voltage-gated potassium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Kappa-theraphotoxin-Ps1a
Short name:
Kappa-TRTX-Ps1a
Alternative name(s):
PaTX1
Phrixotoxin-1
OrganismiParaphysa scrofa (Chilean copper tarantula) (Phrixotrichus auratus)
Taxonomic identifieri269635 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeMygalomorphaeTheraphosidaeParaphysa

Organism-specific databases

ArachnoServeriAS000399. kappa-theraphotoxin-Ps1a.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Peptidei1 – 2929Kappa-theraphotoxin-Ps1aPRO_0000044555Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi2 ↔ 161 Publication
Disulfide bondi9 ↔ 211 Publication
Disulfide bondi15 ↔ 251 Publication
Modified residuei29 – 291Isoleucine amide1 Publication

Keywords - PTMi

Amidation, Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

1
29
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi20 – 267Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1V7FNMR-A1-29[»]
ProteinModelPortaliP61230.
SMRiP61230. Positions 1-29.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61230.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similaritiesi

Belongs to the phrixotoxin family.Curated

Keywords - Domaini

Knottin

Sequencei

Sequence statusi: Complete.

P61230-1 [UniParc]FASTAAdd to basket

« Hide

        10         20 
YCQKWMWTCD SARKCCEGLV CRLWCKKII
Length:29
Mass (Da):3,555
Last modified:May 10, 2004 - v1
Checksum:i3B80CAB68C1B9B36
GO

Mass spectrometryi

Molecular mass is 3547.60 Da from positions 1 - 29. Determined by ESI. 1 Publication

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1V7FNMR-A1-29[»]
ProteinModelPortaliP61230.
SMRiP61230. Positions 1-29.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

ArachnoServeriAS000399. kappa-theraphotoxin-Ps1a.

Miscellaneous databases

EvolutionaryTraceiP61230.

Family and domain databases

ProtoNetiSearch...

Publicationsi

  1. "Effects of phrixotoxins on the Kv4 family of potassium channels and implications for the role of Ito1 in cardiac electrogenesis."
    Diochot S., Drici M.-D., Moinier D., Fink M., Lazdunski M.
    Br. J. Pharmacol. 126:251-263(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, FUNCTION, MASS SPECTROMETRY.
  2. "Solution structure of Phrixotoxin 1, a specific peptide inhibitor of Kv4 potassium channels from the venom of the theraphosid spider Phrixotrichus auratus."
    Chagot B., Escoubas P., Villegas E., Bernard C., Ferrat G., Corzo G., Lazdunski M., Darbon H.
    Protein Sci. 13:1197-1208(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR, SYNTHESIS, DISULFIDE BONDS, AMIDATION AT ILE-29.

Entry informationi

Entry nameiTXP1_PARSR
AccessioniPrimary (citable) accession number: P61230
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: December 9, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Miscellaneous

This toxin does not inhibit Kv1/KCNA, Kv2/KCNB, Kv3/KCNC, and Kv11/KCNH channels.1 Publication

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.