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P61226

- RAP2B_MOUSE

UniProt

P61226 - RAP2B_MOUSE

Protein

Ras-related protein Rap-2b

Gene

Rap2b

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (10 May 2004)
      Previous versions | rss
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    Functioni

    Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. Involved in EGFR and CHRM3 signaling pathways through stimulation of PLCE1. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May regulate membrane vesiculation in red blood cells.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 178GTPBy similarity
    Nucleotide bindingi57 – 615GTPBy similarity
    Nucleotide bindingi116 – 1194GTPBy similarity

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-KW
    2. protein domain specific binding Source: MGI

    GO - Biological processi

    1. GTP catabolic process Source: InterPro
    2. negative regulation of cell migration Source: MGI
    3. positive regulation of protein autophosphorylation Source: MGI
    4. Rap protein signal transduction Source: UniProtKB
    5. regulation of protein tyrosine kinase activity Source: UniProtKB

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ras-related protein Rap-2b
    Gene namesi
    Name:Rap2b
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:1921262. Rap2b.

    Subcellular locationi

    Recycling endosome membrane 1 Publication; Lipid-anchor 1 Publication; Cytoplasmic side 1 Publication
    Note: Associated with red blood cells-released vesicles.By similarity

    GO - Cellular componenti

    1. cytosol Source: MGI
    2. membrane Source: MGI
    3. recycling endosome Source: MGI
    4. recycling endosome membrane Source: UniProtKB

    Keywords - Cellular componenti

    Endosome, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi176 – 1761C → G: Reduced association with the recycling endosome membranes and loss of TNIK activation; when associated with G-177. 1 Publication
    Mutagenesisi177 – 1771C → G: Reduced association with the recycling endosome membranes and loss of TNIK activation; when associated with G-176. 1 Publication
    Mutagenesisi180 – 1801C → A: Loss of association with membranes. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 180180Ras-related protein Rap-2bPRO_0000030217Add
    BLAST
    Propeptidei181 – 1833Removed in mature formBy similarityPRO_0000030218

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei180 – 1801Cysteine methyl esterBy similarity
    Lipidationi180 – 1801S-geranylgeranyl cysteineBy similarity

    Post-translational modificationi

    Palmitoylated. Palmitoylation is required for association with recycling endosome membranes and activation of TNIK.1 Publication

    Keywords - PTMi

    Lipoprotein, Methylation, Palmitate, Prenylation

    Proteomic databases

    MaxQBiP61226.
    PaxDbiP61226.
    PRIDEiP61226.

    PTM databases

    PhosphoSiteiP61226.

    Expressioni

    Gene expression databases

    ArrayExpressiP61226.
    BgeeiP61226.
    CleanExiMM_RAP2B.
    GenevestigatoriP61226.

    Interactioni

    Subunit structurei

    Interacts with PLCE1. Interacts with SGSM1, SGSM2 and SGSM3 By similarity. The GTP-bound form of RAP2B interacts with RUNDC3A.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi216422. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP61226.
    SMRiP61226. Positions 1-167.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi32 – 409Effector regionCurated

    Domaini

    The effector domain mediates the interaction with RUNDC3A.1 Publication

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Ras family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    HOGENOMiHOG000233973.
    HOVERGENiHBG009351.
    InParanoidiP61226.
    KOiK07838.
    OMAiPNGDEQC.
    OrthoDBiEOG7QVM41.
    PhylomeDBiP61226.
    TreeFamiTF313014.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR020849. Small_GTPase_Ras.
    [Graphical view]
    PANTHERiPTHR24070. PTHR24070. 1 hit.
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00173. RAS. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51421. RAS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P61226-1 [UniParc]FASTAAdd to Basket

    « Hide

    MREYKVVVLG SGGVGKSALT VQFVTGSFIE KYDPTIEDFY RKEIEVDSSP    50
    SVLEILDTAG TEQFASMRDL YIKNGQGFIL VYSLVNQQSF QDIKPMRDQI 100
    IRVKRYERVP MILVGNKVDL EGEREVSYGE GKALAEEWSC PFMETSAKNK 150
    ASVDELFAEI VRQMNYAAQP NGDEGCCSAC VIL 183
    Length:183
    Mass (Da):20,504
    Last modified:May 10, 2004 - v1
    Checksum:iA1139C2D5E7F5865
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK014462 mRNA. Translation: BAB29368.1.
    BC032168 mRNA. Translation: AAH32168.1.
    BC046528 mRNA. Translation: AAH46528.1.
    CCDSiCCDS17378.1.
    RefSeqiNP_082988.1. NM_028712.4.
    UniGeneiMm.422674.
    Mm.477648.

    Genome annotation databases

    EnsembliENSMUST00000049064; ENSMUSP00000038841; ENSMUSG00000036894.
    GeneIDi74012.
    KEGGimmu:74012.
    UCSCiuc008pjl.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK014462 mRNA. Translation: BAB29368.1 .
    BC032168 mRNA. Translation: AAH32168.1 .
    BC046528 mRNA. Translation: AAH46528.1 .
    CCDSi CCDS17378.1.
    RefSeqi NP_082988.1. NM_028712.4.
    UniGenei Mm.422674.
    Mm.477648.

    3D structure databases

    ProteinModelPortali P61226.
    SMRi P61226. Positions 1-167.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 216422. 1 interaction.

    PTM databases

    PhosphoSitei P61226.

    Proteomic databases

    MaxQBi P61226.
    PaxDbi P61226.
    PRIDEi P61226.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000049064 ; ENSMUSP00000038841 ; ENSMUSG00000036894 .
    GeneIDi 74012.
    KEGGi mmu:74012.
    UCSCi uc008pjl.1. mouse.

    Organism-specific databases

    CTDi 5912.
    MGIi MGI:1921262. Rap2b.

    Phylogenomic databases

    eggNOGi COG1100.
    HOGENOMi HOG000233973.
    HOVERGENi HBG009351.
    InParanoidi P61226.
    KOi K07838.
    OMAi PNGDEQC.
    OrthoDBi EOG7QVM41.
    PhylomeDBi P61226.
    TreeFami TF313014.

    Miscellaneous databases

    ChiTaRSi RAP2B. mouse.
    NextBioi 339528.
    PROi P61226.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P61226.
    Bgeei P61226.
    CleanExi MM_RAP2B.
    Genevestigatori P61226.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR020849. Small_GTPase_Ras.
    [Graphical view ]
    PANTHERi PTHR24070. PTHR24070. 1 hit.
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00173. RAS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51421. RAS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryonic head.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and FVB/N.
      Tissue: Cerebellum and Mammary gland.
    3. Lubec G., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 6-16; 43-68 AND 151-162, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain.
    4. "Identification of a specific effector of the small GTP-binding protein Rap2."
      Janoueix-Lerosey I., Pasheva E., de Tand M.-F., Tavitian A., de Gunzburg J.
      Eur. J. Biochem. 252:290-298(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RUNDC3A, DOMAIN.
    5. Cited for: FUNCTION, SUBCELLULAR LOCATION, PALMITOYLATION, MUTAGENESIS OF CYS-176; CYS-177 AND CYS-180.

    Entry informationi

    Entry nameiRAP2B_MOUSE
    AccessioniPrimary (citable) accession number: P61226
    Secondary accession number(s): P17964, Q96EG5, Q9CXG0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2004
    Last sequence update: May 10, 2004
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3