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P61225 (RAP2B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ras-related protein Rap-2b
Gene names
Name:RAP2B
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length183 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. Involved in EGFR and CHRM3 signaling pathways through stimulation of PLCE1. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May regulate membrane vesiculation in red blood cells. Ref.6 Ref.8 Ref.9

Subunit structure

Interacts with PLCE1. Interacts with SGSM1, SGSM2 and SGSM3. The GTP-bound form of RAP2B interacts with RUNDC3A By similarity. Ref.7 Ref.10

Subcellular location

Recycling endosome membrane; Lipid-anchor; Cytoplasmic side. Note: Associated with red blood cells-released vesicles. Ref.9

Tissue specificity

Expressed in red blood cells (at protein level). Ref.9

Domain

The effector domain mediates the interaction with RUNDC3A By similarity.

Post-translational modification

Palmitoylated. Palmitoylation is required for association with recycling endosome membranes and activation of TNIK By similarity.

Sequence similarities

Belongs to the small GTPase superfamily. Ras family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 180180Ras-related protein Rap-2b
PRO_0000030215
Propeptide181 – 1833Removed in mature form
PRO_0000030216

Regions

Nucleotide binding10 – 178GTP By similarity
Nucleotide binding57 – 615GTP By similarity
Nucleotide binding116 – 1194GTP By similarity
Motif32 – 409Effector region Probable

Amino acid modifications

Modified residue1801Cysteine methyl ester Probable
Lipidation1801S-geranylgeranyl cysteine Ref.5

Experimental info

Sequence conflict1701P → S in CAA37178. Ref.1
Sequence conflict1701P → S no nucleotide entry Ref.2

Sequences

Sequence LengthMass (Da)Tools
P61225 [UniParc].

Last modified May 10, 2004. Version 1.
Checksum: A1139C2D5E7F5865

FASTA18320,504
        10         20         30         40         50         60 
MREYKVVVLG SGGVGKSALT VQFVTGSFIE KYDPTIEDFY RKEIEVDSSP SVLEILDTAG 

        70         80         90        100        110        120 
TEQFASMRDL YIKNGQGFIL VYSLVNQQSF QDIKPMRDQI IRVKRYERVP MILVGNKVDL 

       130        140        150        160        170        180 
EGEREVSYGE GKALAEEWSC PFMETSAKNK ASVDELFAEI VRQMNYAAQP NGDEGCCSAC 


VIL 

« Hide

References

« Hide 'large scale' references
[1]"cDNA sequence of a new ras-related gene (rap2b) isolated from human platelets with sequence homology to rap2."
Farrell F.X., Ohmstede C.A., Reep B.R., Lapetina E.G.
Nucleic Acids Res. 18:4281-4281(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Platelet.
[2]"RAP2B: a RAS-related GTP-binding protein from platelets."
Ohmstede C.A., Farrell F.X., Reep B.R., Clemetson K.J., Lapetina E.G.
Proc. Natl. Acad. Sci. U.S.A. 87:6527-6531(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Platelet.
[3]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Puhl H.L. III, Ikeda S.R., Aronstam R.S.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Placenta.
[5]"Prenyl group identification of rap2 proteins: a ras superfamily member other than ras that is farnesylated."
Farrell F.X., Yamamoto K., Lapetina E.G.
Biochem. J. 289:349-355(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: ISOPRENYLATION AT CYS-180.
[6]"Stimulation of phospholipase C-epsilon by the M3 muscarinic acetylcholine receptor mediated by cyclic AMP and the GTPase Rap2B."
Evellin S., Nolte J., Tysack K., vom Dorp F., Thiel M., Weernink P.A.O., Jakobs K.H., Webb E.J., Lomasney J.W., Schmidt M.
J. Biol. Chem. 277:16805-16813(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Differential roles of Ras and Rap1 in growth factor-dependent activation of phospholipase C epsilon."
Song C., Satoh T., Edamatsu H., Wu D., Tadano M., Gao X., Kataoka T.
Oncogene 21:8105-8113(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PLCE1.
[8]"Rap2B-dependent stimulation of phospholipase C-epsilon by epidermal growth factor receptor mediated by c-Src phosphorylation of RasGRP3."
Stope M.B., Vom Dorp F., Szatkowski D., Boehm A., Keiper M., Nolte J., Oude Weernink P.A., Rosskopf D., Evellin S., Jakobs K.H., Schmidt M.
Mol. Cell. Biol. 24:4664-4676(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"Rap2, but not Rap1 GTPase is expressed in human red blood cells and is involved in vesiculation."
Greco F., Ciana A., Pietra D., Balduini C., Minetti G., Torti M.
Biochim. Biophys. Acta 1763:330-335(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[10]"Identification of three novel proteins (SGSM1, 2, 3) which modulate small G protein (RAP and RAB)-mediated signaling pathway."
Yang H., Sasaki T., Minoshima S., Shimizu N.
Genomics 90:249-260(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SGSM1; SGSM2 AND SGSM3.
[11]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X52987 mRNA. Translation: CAA37178.1.
AF493915 mRNA. Translation: AAM12629.1.
BC012362 mRNA. Translation: AAH12362.1.
CCDSCCDS3170.1.
PIRTVHUR2. A33121.
RefSeqNP_002877.2. NM_002886.3.
UniGeneHs.700713.
Hs.98643.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1N4PX-ray2.65M/N180-183[»]
1N4QX-ray2.40M/N/O/P/Q/R180-183[»]
1N4RX-ray2.80M/N/O/P/Q/R180-183[»]
ProteinModelPortalP61225.
SMRP61225. Positions 1-167.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid111847. 10 interactions.
IntActP61225. 4 interactions.
MINTMINT-5000383.
STRING9606.ENSP00000319096.

PTM databases

PhosphoSiteP61225.

Polymorphism databases

DMDM47117762.

Proteomic databases

MaxQBP61225.
PaxDbP61225.
PeptideAtlasP61225.
PRIDEP61225.

Protocols and materials databases

DNASU5912.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000323534; ENSP00000319096; ENSG00000181467.
GeneID5912.
KEGGhsa:5912.
UCSCuc003ezr.3. human.

Organism-specific databases

CTD5912.
GeneCardsGC03P152880.
HGNCHGNC:9862. RAP2B.
MIM179541. gene.
neXtProtNX_P61225.
PharmGKBPA34223.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1100.
HOGENOMHOG000233973.
HOVERGENHBG009351.
InParanoidP61225.
KOK07838.
OMAPNGDEQC.
OrthoDBEOG7QVM41.
PhylomeDBP61225.
TreeFamTF313014.

Gene expression databases

ArrayExpressP61225.
BgeeP61225.
CleanExHS_RAP2B.
GenevestigatorP61225.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERPTHR24070. PTHR24070. 1 hit.
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00173. RAS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSRAP2B. human.
GeneWikiRAP2B.
GenomeRNAi5912.
NextBio23008.
PROP61225.
SOURCESearch...

Entry information

Entry nameRAP2B_HUMAN
AccessionPrimary (citable) accession number: P61225
Secondary accession number(s): P17964, Q96EG5, Q9CXG0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: July 9, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM