P61225 (RAP2B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras-related protein Rap-2b

Gene names

Name:

RAP2B

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	183 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. Involved in EGFR and CHRM3 signaling pathways through stimulation of PLCE1. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May regulate membrane vesiculation in red blood cells. Ref.6 Ref.8 Ref.9
Subunit structure	Interacts with PLCE1. Interacts with SGSM1, SGSM2 and SGSM3. The GTP-bound form of RAP2B interacts with RUNDC3A By similarity. Ref.7 Ref.10
Subcellular location	Recycling endosome membrane; Lipid-anchor; Cytoplasmic side. Note: Associated with red blood cells-released vesicles. Ref.9
Tissue specificity	Expressed in red blood cells (at protein level). Ref.9
Domain	The effector domain mediates the interaction with RUNDC3A By similarity.
Post-translational modification	Palmitoylated. Palmitoylation is required for association with recycling endosome membranes and activation of TNIK By similarity.
Sequence similarities	Belongs to the small GTPase superfamily. Ras family.

Ontologies

Keywords
Cellular component	Endosome Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Palmitate Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Rap protein signal transduction Inferred from sequence or structural similarity. Source: UniProtKB regulation of protein tyrosine kinase activity Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	recycling endosome membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	GTP binding Traceable author statement. Source: ProtInc GTPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 180	180	Ras-related protein Rap-2b		PRO_0000030215
	Propeptide	181 – 183	3	Removed in mature form		PRO_0000030216
Regions
	Nucleotide binding	10 – 17	8	GTP By similarity
	Nucleotide binding	57 – 61	5	GTP By similarity
	Nucleotide binding	116 – 119	4	GTP By similarity
	Motif	32 – 40	9	Effector region Probable
Amino acid modifications
	Modified residue	180	1	Cysteine methyl ester Probable
	Lipidation	180	1	S-geranylgeranyl cysteine Ref.5
Experimental info
	Sequence conflict	170	1	P → S in CAA37178. Ref.1
	Sequence conflict	170	1	P → S no nucleotide entry Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P61225 [UniParc]. Last modified May 10, 2004. Version 1. Checksum: A1139C2D5E7F5865	FASTA	183	20,504
	10 20 30 40 50 60 MREYKVVVLG SGGVGKSALT VQFVTGSFIE KYDPTIEDFY RKEIEVDSSP SVLEILDTAG 70 80 90 100 110 120 TEQFASMRDL YIKNGQGFIL VYSLVNQQSF QDIKPMRDQI IRVKRYERVP MILVGNKVDL 130 140 150 160 170 180 EGEREVSYGE GKALAEEWSC PFMETSAKNK ASVDELFAEI VRQMNYAAQP NGDEGCCSAC VIL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA sequence of a new ras-related gene (rap2b) isolated from human platelets with sequence homology to rap2." Farrell F.X., Ohmstede C.A., Reep B.R., Lapetina E.G. Nucleic Acids Res. 18:4281-4281(1990) [PubMed: 2115998] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Platelet.
[2]	"RAP2B: a RAS-related GTP-binding protein from platelets." Ohmstede C.A., Farrell F.X., Reep B.R., Clemetson K.J., Lapetina E.G. Proc. Natl. Acad. Sci. U.S.A. 87:6527-6531(1990) [PubMed: 2118648] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Platelet.
[3]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[5]	"Prenyl group identification of rap2 proteins: a ras superfamily member other than ras that is farnesylated." Farrell F.X., Yamamoto K., Lapetina E.G. Biochem. J. 289:349-355(1993) [PubMed: 8424780] [Abstract] Cited for: ISOPRENYLATION AT CYS-180.
[6]	"Stimulation of phospholipase C-epsilon by the M3 muscarinic acetylcholine receptor mediated by cyclic AMP and the GTPase Rap2B." Evellin S., Nolte J., Tysack K., vom Dorp F., Thiel M., Weernink P.A.O., Jakobs K.H., Webb E.J., Lomasney J.W., Schmidt M. J. Biol. Chem. 277:16805-16813(2002) [PubMed: 11877431] [Abstract] Cited for: FUNCTION.
[7]	"Differential roles of Ras and Rap1 in growth factor-dependent activation of phospholipase C epsilon." Song C., Satoh T., Edamatsu H., Wu D., Tadano M., Gao X., Kataoka T. Oncogene 21:8105-8113(2002) [PubMed: 12444546] [Abstract] Cited for: INTERACTION WITH PLCE1.
[8]	"Rap2B-dependent stimulation of phospholipase C-epsilon by epidermal growth factor receptor mediated by c-Src phosphorylation of RasGRP3." Stope M.B., Vom Dorp F., Szatkowski D., Boehm A., Keiper M., Nolte J., Oude Weernink P.A., Rosskopf D., Evellin S., Jakobs K.H., Schmidt M. Mol. Cell. Biol. 24:4664-4676(2004) [PubMed: 15143162] [Abstract] Cited for: FUNCTION.
[9]	"Rap2, but not Rap1 GTPase is expressed in human red blood cells and is involved in vesiculation." Greco F., Ciana A., Pietra D., Balduini C., Minetti G., Torti M. Biochim. Biophys. Acta 1763:330-335(2006) [PubMed: 16540189] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[10]	"Identification of three novel proteins (SGSM1, 2, 3) which modulate small G protein (RAP and RAB)-mediated signaling pathway." Yang H., Sasaki T., Minoshima S., Shimizu N. Genomics 90:249-260(2007) [PubMed: 17509819] [Abstract] Cited for: INTERACTION WITH SGSM1; SGSM2 AND SGSM3.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBL GenBank DDBJ	X52987 mRNA. Translation: CAA37178.1. AF493915 mRNA. Translation: AAM12629.1. BC012362 mRNA. Translation: AAH12362.1.
IPI	IPI00018364.
PIR	TVHUR2. A33121.
RefSeq	NP_002877.2. NM_002886.2.
UniGene	Hs.98643.
3D structure databases
ProteinModelPortal	P61225.
SMR	P61225. Positions 1-167.
ModBase	Search...
Protein-protein interaction databases
IntAct	P61225. 2 interactions.
MINT	MINT-5000383.
STRING	P61225.
PTM databases
PhosphoSite	P61225.
Polymorphism databases
DMDM	47117762.
Proteomic databases
PeptideAtlas	P61225.
PRIDE	P61225.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000323534; ENSP00000319096; ENSG00000181467.
GeneID	5912.
KEGG	hsa:5912.
UCSC	uc003ezr.1. human.
Organism-specific databases
CTD	5912.
GeneCards	GC03P152880.
H-InvDB	HIX0003788.
HGNC	HGNC:9862. RAP2B.
MIM	179541. gene.
neXtProt	NX_P61225.
PharmGKB	PA34223.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG18848.
HOGENOM	HBG745225.
HOVERGEN	HBG009351.
InParanoid	P61225.
OMA	SQPNGDD.
OrthoDB	EOG4P2Q3G.
PhylomeDB	P61225.
Gene expression databases
ArrayExpress	P61225.
Bgee	P61225.
CleanEx	HS_RAP2B.
Genevestigator	P61225.
GermOnline	ENSG00000181467. Homo sapiens.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR020849. Small_GTPase_Ras. [Graphical view]
KO	K07838.
PANTHER	PTHR24070. PTHR24070. 1 hit.
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00173. RAS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. Small_GTP. 1 hit.
PROSITE	PS51421. RAS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	23008.
SOURCE	Search...

Entry information

Entry name

RAP2B_HUMAN

Accession

Primary (citable) accession number: P61225
Secondary accession number(s): P17964, Q96EG5, Q9CXG0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	May 10, 2004
Last modified:	January 25, 2012
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.