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Protein

Ras-related protein Rap-2b

Gene

RAP2B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. Involved in EGFR and CHRM3 signaling pathways through stimulation of PLCE1. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May regulate membrane vesiculation in red blood cells.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 178GTPBy similarity
Nucleotide bindingi57 – 615GTPBy similarity
Nucleotide bindingi116 – 1194GTPBy similarity

GO - Molecular functioni

  • GDP binding Source: UniProtKB
  • GTP binding Source: UniProtKB

GO - Biological processi

  • negative regulation of cell migration Source: Ensembl
  • platelet activation Source: UniProtKB
  • platelet aggregation Source: UniProtKB
  • positive regulation of protein autophosphorylation Source: Ensembl
  • Rap protein signal transduction Source: UniProtKB
  • regulation of protein tyrosine kinase activity Source: UniProtKB
  • signal transduction Source: ProtInc
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rap-2b
Gene namesi
Name:RAP2B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:9862. RAP2B.

Subcellular locationi

GO - Cellular componenti

  • cell-cell contact zone Source: UniProtKB
  • cytosol Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • membrane Source: UniProtKB
  • membrane raft Source: UniProtKB
  • plasma membrane Source: UniProtKB
  • recycling endosome membrane Source: UniProtKB
  • tight junction Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA34223.

Polymorphism and mutation databases

DMDMi47117762.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 180180Ras-related protein Rap-2bPRO_0000030215Add
BLAST
Propeptidei181 – 1833Removed in mature formPRO_0000030216

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei180 – 1801Cysteine methyl esterCurated
Lipidationi180 – 1801S-geranylgeranyl cysteine1 Publication

Post-translational modificationi

Palmitoylated. Palmitoylation is required for association with recycling endosome membranes and activation of TNIK (By similarity).By similarity

Keywords - PTMi

Lipoprotein, Methylation, Palmitate, Prenylation

Proteomic databases

MaxQBiP61225.
PaxDbiP61225.
PeptideAtlasiP61225.
PRIDEiP61225.

PTM databases

PhosphoSiteiP61225.

Expressioni

Tissue specificityi

Expressed in red blood cells (at protein level).1 Publication

Gene expression databases

BgeeiP61225.
CleanExiHS_RAP2B.
GenevestigatoriP61225.

Interactioni

Subunit structurei

Interacts with PLCE1. Interacts with SGSM1, SGSM2 and SGSM3. The GTP-bound form of RAP2B interacts with RUNDC3A (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CAMK2BQ135543EBI-750871,EBI-1058722
RASSF5Q8WWW03EBI-750871,EBI-367390
RUNDC3AQ59EK94EBI-750871,EBI-747225

Protein-protein interaction databases

BioGridi111847. 13 interactions.
IntActiP61225. 6 interactions.
MINTiMINT-5000383.
STRINGi9606.ENSP00000319096.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1N4PX-ray2.65M/N180-183[»]
1N4QX-ray2.40M/N/O/P/Q/R180-183[»]
1N4RX-ray2.80M/N/O/P/Q/R180-183[»]
ProteinModelPortaliP61225.
SMRiP61225. Positions 1-167.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi32 – 409Effector regionCurated

Domaini

The effector domain mediates the interaction with RUNDC3A.By similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00780000121857.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiP61225.
KOiK07838.
OMAiPNGDEQC.
OrthoDBiEOG7QVM41.
PhylomeDBiP61225.
TreeFamiTF313014.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00173. RAS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61225-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREYKVVVLG SGGVGKSALT VQFVTGSFIE KYDPTIEDFY RKEIEVDSSP
60 70 80 90 100
SVLEILDTAG TEQFASMRDL YIKNGQGFIL VYSLVNQQSF QDIKPMRDQI
110 120 130 140 150
IRVKRYERVP MILVGNKVDL EGEREVSYGE GKALAEEWSC PFMETSAKNK
160 170 180
ASVDELFAEI VRQMNYAAQP NGDEGCCSAC VIL
Length:183
Mass (Da):20,504
Last modified:May 10, 2004 - v1
Checksum:iA1139C2D5E7F5865
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti170 – 1701P → S in CAA37178 (PubMed:2115998).Curated
Sequence conflicti170 – 1701P → S no nucleotide entry (PubMed:2118648).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52987 mRNA. Translation: CAA37178.1.
AF493915 mRNA. Translation: AAM12629.1.
BC012362 mRNA. Translation: AAH12362.1.
CCDSiCCDS3170.1.
PIRiA33121. TVHUR2.
RefSeqiNP_002877.2. NM_002886.3.
UniGeneiHs.700713.
Hs.98643.

Genome annotation databases

EnsembliENST00000323534; ENSP00000319096; ENSG00000181467.
GeneIDi5912.
KEGGihsa:5912.
UCSCiuc003ezr.3. human.

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52987 mRNA. Translation: CAA37178.1.
AF493915 mRNA. Translation: AAM12629.1.
BC012362 mRNA. Translation: AAH12362.1.
CCDSiCCDS3170.1.
PIRiA33121. TVHUR2.
RefSeqiNP_002877.2. NM_002886.3.
UniGeneiHs.700713.
Hs.98643.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1N4PX-ray2.65M/N180-183[»]
1N4QX-ray2.40M/N/O/P/Q/R180-183[»]
1N4RX-ray2.80M/N/O/P/Q/R180-183[»]
ProteinModelPortaliP61225.
SMRiP61225. Positions 1-167.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111847. 13 interactions.
IntActiP61225. 6 interactions.
MINTiMINT-5000383.
STRINGi9606.ENSP00000319096.

PTM databases

PhosphoSiteiP61225.

Polymorphism and mutation databases

DMDMi47117762.

Proteomic databases

MaxQBiP61225.
PaxDbiP61225.
PeptideAtlasiP61225.
PRIDEiP61225.

Protocols and materials databases

DNASUi5912.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000323534; ENSP00000319096; ENSG00000181467.
GeneIDi5912.
KEGGihsa:5912.
UCSCiuc003ezr.3. human.

Organism-specific databases

CTDi5912.
GeneCardsiGC03P152880.
HGNCiHGNC:9862. RAP2B.
MIMi179541. gene.
neXtProtiNX_P61225.
PharmGKBiPA34223.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00780000121857.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiP61225.
KOiK07838.
OMAiPNGDEQC.
OrthoDBiEOG7QVM41.
PhylomeDBiP61225.
TreeFamiTF313014.

Miscellaneous databases

ChiTaRSiRAP2B. human.
GeneWikiiRAP2B.
GenomeRNAii5912.
NextBioi23008.
PROiP61225.
SOURCEiSearch...

Gene expression databases

BgeeiP61225.
CleanExiHS_RAP2B.
GenevestigatoriP61225.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00173. RAS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA sequence of a new ras-related gene (rap2b) isolated from human platelets with sequence homology to rap2."
    Farrell F.X., Ohmstede C.A., Reep B.R., Lapetina E.G.
    Nucleic Acids Res. 18:4281-4281(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Platelet.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Platelet.
  3. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
    Puhl H.L. III, Ikeda S.R., Aronstam R.S.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta.
  5. "Prenyl group identification of rap2 proteins: a ras superfamily member other than ras that is farnesylated."
    Farrell F.X., Yamamoto K., Lapetina E.G.
    Biochem. J. 289:349-355(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: ISOPRENYLATION AT CYS-180.
  6. "Stimulation of phospholipase C-epsilon by the M3 muscarinic acetylcholine receptor mediated by cyclic AMP and the GTPase Rap2B."
    Evellin S., Nolte J., Tysack K., vom Dorp F., Thiel M., Weernink P.A.O., Jakobs K.H., Webb E.J., Lomasney J.W., Schmidt M.
    J. Biol. Chem. 277:16805-16813(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Differential roles of Ras and Rap1 in growth factor-dependent activation of phospholipase C epsilon."
    Song C., Satoh T., Edamatsu H., Wu D., Tadano M., Gao X., Kataoka T.
    Oncogene 21:8105-8113(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PLCE1.
  8. "Rap2B-dependent stimulation of phospholipase C-epsilon by epidermal growth factor receptor mediated by c-Src phosphorylation of RasGRP3."
    Stope M.B., Vom Dorp F., Szatkowski D., Boehm A., Keiper M., Nolte J., Oude Weernink P.A., Rosskopf D., Evellin S., Jakobs K.H., Schmidt M.
    Mol. Cell. Biol. 24:4664-4676(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Rap2, but not Rap1 GTPase is expressed in human red blood cells and is involved in vesiculation."
    Greco F., Ciana A., Pietra D., Balduini C., Minetti G., Torti M.
    Biochim. Biophys. Acta 1763:330-335(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  10. "Identification of three novel proteins (SGSM1, 2, 3) which modulate small G protein (RAP and RAB)-mediated signaling pathway."
    Yang H., Sasaki T., Minoshima S., Shimizu N.
    Genomics 90:249-260(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SGSM1; SGSM2 AND SGSM3.
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRAP2B_HUMAN
AccessioniPrimary (citable) accession number: P61225
Secondary accession number(s): P17964, Q96EG5, Q9CXG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: May 27, 2015
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.