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P61225

- RAP2B_HUMAN

UniProt

P61225 - RAP2B_HUMAN

Protein

Ras-related protein Rap-2b

Gene

RAP2B

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (10 May 2004)
      Previous versions | rss
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    Functioni

    Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. Involved in EGFR and CHRM3 signaling pathways through stimulation of PLCE1. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May regulate membrane vesiculation in red blood cells.3 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 178GTPBy similarity
    Nucleotide bindingi57 – 615GTPBy similarity
    Nucleotide bindingi116 – 1194GTPBy similarity

    GO - Molecular functioni

    1. GDP binding Source: UniProt
    2. GTP binding Source: UniProt

    GO - Biological processi

    1. GTP catabolic process Source: InterPro
    2. negative regulation of cell migration Source: Ensembl
    3. positive regulation of protein autophosphorylation Source: Ensembl
    4. Rap protein signal transduction Source: UniProtKB
    5. regulation of protein tyrosine kinase activity Source: UniProtKB
    6. signal transduction Source: ProtInc

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ras-related protein Rap-2b
    Gene namesi
    Name:RAP2B
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 3

    Organism-specific databases

    HGNCiHGNC:9862. RAP2B.

    Subcellular locationi

    Recycling endosome membrane 1 Publication; Lipid-anchor 1 Publication; Cytoplasmic side 1 Publication
    Note: Associated with red blood cells-released vesicles.

    GO - Cellular componenti

    1. cytosol Source: Ensembl
    2. extracellular vesicular exosome Source: UniProt
    3. plasma membrane Source: UniProt
    4. recycling endosome membrane Source: UniProtKB

    Keywords - Cellular componenti

    Endosome, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA34223.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 180180Ras-related protein Rap-2bPRO_0000030215Add
    BLAST
    Propeptidei181 – 1833Removed in mature formPRO_0000030216

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei180 – 1801Cysteine methyl esterCurated
    Lipidationi180 – 1801S-geranylgeranyl cysteine1 Publication

    Post-translational modificationi

    Palmitoylated. Palmitoylation is required for association with recycling endosome membranes and activation of TNIK By similarity.By similarity

    Keywords - PTMi

    Lipoprotein, Methylation, Palmitate, Prenylation

    Proteomic databases

    MaxQBiP61225.
    PaxDbiP61225.
    PeptideAtlasiP61225.
    PRIDEiP61225.

    PTM databases

    PhosphoSiteiP61225.

    Expressioni

    Tissue specificityi

    Expressed in red blood cells (at protein level).1 Publication

    Gene expression databases

    ArrayExpressiP61225.
    BgeeiP61225.
    CleanExiHS_RAP2B.
    GenevestigatoriP61225.

    Interactioni

    Subunit structurei

    Interacts with PLCE1. Interacts with SGSM1, SGSM2 and SGSM3. The GTP-bound form of RAP2B interacts with RUNDC3A By similarity.By similarity

    Protein-protein interaction databases

    BioGridi111847. 10 interactions.
    IntActiP61225. 4 interactions.
    MINTiMINT-5000383.
    STRINGi9606.ENSP00000319096.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1N4PX-ray2.65M/N180-183[»]
    1N4QX-ray2.40M/N/O/P/Q/R180-183[»]
    1N4RX-ray2.80M/N/O/P/Q/R180-183[»]
    ProteinModelPortaliP61225.
    SMRiP61225. Positions 1-167.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi32 – 409Effector regionCurated

    Domaini

    The effector domain mediates the interaction with RUNDC3A.By similarity

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Ras family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    HOGENOMiHOG000233973.
    HOVERGENiHBG009351.
    InParanoidiP61225.
    KOiK07838.
    OMAiPNGDEQC.
    OrthoDBiEOG7QVM41.
    PhylomeDBiP61225.
    TreeFamiTF313014.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR020849. Small_GTPase_Ras.
    [Graphical view]
    PANTHERiPTHR24070. PTHR24070. 1 hit.
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00173. RAS. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51421. RAS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P61225-1 [UniParc]FASTAAdd to Basket

    « Hide

    MREYKVVVLG SGGVGKSALT VQFVTGSFIE KYDPTIEDFY RKEIEVDSSP    50
    SVLEILDTAG TEQFASMRDL YIKNGQGFIL VYSLVNQQSF QDIKPMRDQI 100
    IRVKRYERVP MILVGNKVDL EGEREVSYGE GKALAEEWSC PFMETSAKNK 150
    ASVDELFAEI VRQMNYAAQP NGDEGCCSAC VIL 183
    Length:183
    Mass (Da):20,504
    Last modified:May 10, 2004 - v1
    Checksum:iA1139C2D5E7F5865
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti170 – 1701P → S in CAA37178. (PubMed:2115998)Curated
    Sequence conflicti170 – 1701P → S no nucleotide entry (PubMed:2118648)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52987 mRNA. Translation: CAA37178.1.
    AF493915 mRNA. Translation: AAM12629.1.
    BC012362 mRNA. Translation: AAH12362.1.
    CCDSiCCDS3170.1.
    PIRiA33121. TVHUR2.
    RefSeqiNP_002877.2. NM_002886.3.
    UniGeneiHs.700713.
    Hs.98643.

    Genome annotation databases

    EnsembliENST00000323534; ENSP00000319096; ENSG00000181467.
    GeneIDi5912.
    KEGGihsa:5912.
    UCSCiuc003ezr.3. human.

    Polymorphism databases

    DMDMi47117762.

    Cross-referencesi

    Web resourcesi

    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52987 mRNA. Translation: CAA37178.1 .
    AF493915 mRNA. Translation: AAM12629.1 .
    BC012362 mRNA. Translation: AAH12362.1 .
    CCDSi CCDS3170.1.
    PIRi A33121. TVHUR2.
    RefSeqi NP_002877.2. NM_002886.3.
    UniGenei Hs.700713.
    Hs.98643.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1N4P X-ray 2.65 M/N 180-183 [» ]
    1N4Q X-ray 2.40 M/N/O/P/Q/R 180-183 [» ]
    1N4R X-ray 2.80 M/N/O/P/Q/R 180-183 [» ]
    ProteinModelPortali P61225.
    SMRi P61225. Positions 1-167.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111847. 10 interactions.
    IntActi P61225. 4 interactions.
    MINTi MINT-5000383.
    STRINGi 9606.ENSP00000319096.

    PTM databases

    PhosphoSitei P61225.

    Polymorphism databases

    DMDMi 47117762.

    Proteomic databases

    MaxQBi P61225.
    PaxDbi P61225.
    PeptideAtlasi P61225.
    PRIDEi P61225.

    Protocols and materials databases

    DNASUi 5912.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000323534 ; ENSP00000319096 ; ENSG00000181467 .
    GeneIDi 5912.
    KEGGi hsa:5912.
    UCSCi uc003ezr.3. human.

    Organism-specific databases

    CTDi 5912.
    GeneCardsi GC03P152880.
    HGNCi HGNC:9862. RAP2B.
    MIMi 179541. gene.
    neXtProti NX_P61225.
    PharmGKBi PA34223.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1100.
    HOGENOMi HOG000233973.
    HOVERGENi HBG009351.
    InParanoidi P61225.
    KOi K07838.
    OMAi PNGDEQC.
    OrthoDBi EOG7QVM41.
    PhylomeDBi P61225.
    TreeFami TF313014.

    Miscellaneous databases

    ChiTaRSi RAP2B. human.
    GeneWikii RAP2B.
    GenomeRNAii 5912.
    NextBioi 23008.
    PROi P61225.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P61225.
    Bgeei P61225.
    CleanExi HS_RAP2B.
    Genevestigatori P61225.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR020849. Small_GTPase_Ras.
    [Graphical view ]
    PANTHERi PTHR24070. PTHR24070. 1 hit.
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00173. RAS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51421. RAS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA sequence of a new ras-related gene (rap2b) isolated from human platelets with sequence homology to rap2."
      Farrell F.X., Ohmstede C.A., Reep B.R., Lapetina E.G.
      Nucleic Acids Res. 18:4281-4281(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Platelet.
    2. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Platelet.
    3. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
      Puhl H.L. III, Ikeda S.R., Aronstam R.S.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Placenta.
    5. "Prenyl group identification of rap2 proteins: a ras superfamily member other than ras that is farnesylated."
      Farrell F.X., Yamamoto K., Lapetina E.G.
      Biochem. J. 289:349-355(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: ISOPRENYLATION AT CYS-180.
    6. "Stimulation of phospholipase C-epsilon by the M3 muscarinic acetylcholine receptor mediated by cyclic AMP and the GTPase Rap2B."
      Evellin S., Nolte J., Tysack K., vom Dorp F., Thiel M., Weernink P.A.O., Jakobs K.H., Webb E.J., Lomasney J.W., Schmidt M.
      J. Biol. Chem. 277:16805-16813(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Differential roles of Ras and Rap1 in growth factor-dependent activation of phospholipase C epsilon."
      Song C., Satoh T., Edamatsu H., Wu D., Tadano M., Gao X., Kataoka T.
      Oncogene 21:8105-8113(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PLCE1.
    8. "Rap2B-dependent stimulation of phospholipase C-epsilon by epidermal growth factor receptor mediated by c-Src phosphorylation of RasGRP3."
      Stope M.B., Vom Dorp F., Szatkowski D., Boehm A., Keiper M., Nolte J., Oude Weernink P.A., Rosskopf D., Evellin S., Jakobs K.H., Schmidt M.
      Mol. Cell. Biol. 24:4664-4676(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Rap2, but not Rap1 GTPase is expressed in human red blood cells and is involved in vesiculation."
      Greco F., Ciana A., Pietra D., Balduini C., Minetti G., Torti M.
      Biochim. Biophys. Acta 1763:330-335(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    10. "Identification of three novel proteins (SGSM1, 2, 3) which modulate small G protein (RAP and RAB)-mediated signaling pathway."
      Yang H., Sasaki T., Minoshima S., Shimizu N.
      Genomics 90:249-260(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SGSM1; SGSM2 AND SGSM3.
    11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRAP2B_HUMAN
    AccessioniPrimary (citable) accession number: P61225
    Secondary accession number(s): P17964, Q96EG5, Q9CXG0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2004
    Last sequence update: May 10, 2004
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 3
      Human chromosome 3: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3