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Protein

Ras-related protein Rap-1b

Gene

RAP1B

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. Plays a role in the establishment of basal endothelial barrier function (By similarity).By similarity

Enzyme regulationi

Activated by guanine nucleotide-exchange factor (GEF) EPAC2 in a cAMP-dependent manner.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189GTPBy similarity
Nucleotide bindingi57 – 615GTPBy similarity
Nucleotide bindingi116 – 1194GTPBy similarity
Nucleotide bindingi147 – 1493GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-BTA-354192. Integrin alphaIIb beta3 signaling.
R-BTA-354194. GRB2:SOS provides linkage to MAPK signaling for Integrins.
R-BTA-372708. p130Cas linkage to MAPK signaling for integrins.
R-BTA-392517. Rap1 signalling.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rap-1b
Alternative name(s):
GTP-binding protein smg p21B
Gene namesi
Name:RAP1B
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 5

Subcellular locationi

  • Cell membrane By similarity
  • Cytoplasmcytosol By similarity
  • Cell junction By similarity

  • Note: May shuttle between plasma membrane and cytosol. Presence of KRIT1 and CDH5 is required for its localization to the cell junction (By similarity).By similarity

GO - Cellular componenti

  • cell-cell junction Source: UniProtKB
  • cytosol Source: UniProtKB-SubCell
  • extracellular exosome Source: Ensembl
  • lipid particle Source: Ensembl
  • membrane Source: UniProtKB
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 181181Ras-related protein Rap-1bPRO_0000030207Add
BLAST
Propeptidei182 – 1843Removed in mature formBy similarityPRO_0000030208

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei179 – 1791Phosphoserine; by PKABy similarity
Modified residuei181 – 1811Cysteine methyl esterBy similarity
Lipidationi181 – 1811S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiP61223.
PRIDEiP61223.

PTM databases

iPTMnetiP61223.

Interactioni

Subunit structurei

Interacts with SGSM1, SGSM2 and SGSM3. Interacts with KRIT1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000011803.

Structurei

3D structure databases

ProteinModelPortaliP61223.
SMRiP61223. Positions 1-166.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni25 – 6743Interaction with KRIT1By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi32 – 409Effector regionCurated

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121857.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiP61223.
KOiK07836.
OMAiRDSTHPR.
OrthoDBiEOG7QVM41.
TreeFamiTF313014.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61223-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDAQQ
60 70 80 90 100
CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI
110 120 130 140 150
LRVKDTDDVP MILVGNKCDL EDERVVGKEQ GQNLARQWNN CAFLESSAKS
160 170 180
KINVNEIFYD LVRQINRKTP VPGKARKKSS CQLL
Length:184
Mass (Da):20,825
Last modified:May 10, 2004 - v1
Checksum:iCE976895E5965224
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33141 mRNA. Translation: AAA30763.1.
BC102330 mRNA. Translation: AAI02331.1.
PIRiA34655.
RefSeqiNP_787018.1. NM_175824.2.
XP_005206506.1. XM_005206449.3.
XP_010803385.1. XM_010805083.1.
XP_010803386.1. XM_010805084.2.
UniGeneiBt.106629.

Genome annotation databases

EnsembliENSBTAT00000011803; ENSBTAP00000011803; ENSBTAG00000008967.
GeneIDi327708.
KEGGibta:327708.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33141 mRNA. Translation: AAA30763.1.
BC102330 mRNA. Translation: AAI02331.1.
PIRiA34655.
RefSeqiNP_787018.1. NM_175824.2.
XP_005206506.1. XM_005206449.3.
XP_010803385.1. XM_010805083.1.
XP_010803386.1. XM_010805084.2.
UniGeneiBt.106629.

3D structure databases

ProteinModelPortaliP61223.
SMRiP61223. Positions 1-166.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000011803.

PTM databases

iPTMnetiP61223.

Proteomic databases

PaxDbiP61223.
PRIDEiP61223.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000011803; ENSBTAP00000011803; ENSBTAG00000008967.
GeneIDi327708.
KEGGibta:327708.

Organism-specific databases

CTDi5908.

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121857.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiP61223.
KOiK07836.
OMAiRDSTHPR.
OrthoDBiEOG7QVM41.
TreeFamiTF313014.

Enzyme and pathway databases

ReactomeiR-BTA-354192. Integrin alphaIIb beta3 signaling.
R-BTA-354194. GRB2:SOS provides linkage to MAPK signaling for Integrins.
R-BTA-372708. p130Cas linkage to MAPK signaling for integrins.
R-BTA-392517. Rap1 signalling.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of smg p21B and identification of smg p21 purified from bovine brain and human platelets as smg p21B."
    Matsui Y., Kikuchi A., Kawata M., Kondo J., Teranishi Y., Takai Y.
    Biochem. Biophys. Res. Commun. 166:1010-1016(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.

Entry informationi

Entry nameiRAP1B_BOVIN
AccessioniPrimary (citable) accession number: P61223
Secondary accession number(s): P09526, Q3T0M4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: June 8, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.