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P61221

- ABCE1_HUMAN

UniProt

P61221 - ABCE1_HUMAN

Protein

ATP-binding cassette sub-family E member 1

Gene

ABCE1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (10 May 2004)
      Previous versions | rss
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    Functioni

    Antagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) by RNase L through direct interaction with RNase L and therefore inhibits its endoribonuclease activity. May play a central role in the regulation of mRNA turnover. Antagonizes the anti-viral effect of the interferon-regulated 2-5A/RNase L pathway. May act as a chaperone for post-translational events during HIV-1 capsid assembly.3 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi110 – 1178ATP 1PROSITE-ProRule annotation
    Nucleotide bindingi379 – 3868ATP 2PROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATPase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. iron-sulfur cluster binding Source: InterPro
    4. ribonuclease inhibitor activity Source: ProtInc

    GO - Biological processi

    1. negative regulation of catalytic activity Source: GOC
    2. response to virus Source: ProtInc
    3. RNA catabolic process Source: ProtInc
    4. viral process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Chaperone

    Keywords - Biological processi

    Host-virus interaction

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-binding cassette sub-family E member 1
    Alternative name(s):
    2'-5'-oligoadenylate-binding protein
    HuHP68
    RNase L inhibitor
    Ribonuclease 4 inhibitor
    Short name:
    RNS4I
    Gene namesi
    Name:ABCE1
    Synonyms:RLI, RNASEL1, RNASELI, RNS4I
    ORF Names:OK/SW-cl.40
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 4

    Organism-specific databases

    HGNCiHGNC:69. ABCE1.

    Subcellular locationi

    Cytoplasm 1 Publication. Mitochondrion 1 Publication
    Note: Localized to clusters of virus formation at the plasma membrane.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. membrane Source: UniProtKB
    3. mitochondrion Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA24404.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 599599ATP-binding cassette sub-family E member 1PRO_0000093316Add
    BLAST

    Proteomic databases

    MaxQBiP61221.
    PaxDbiP61221.
    PeptideAtlasiP61221.
    PRIDEiP61221.

    PTM databases

    PhosphoSiteiP61221.

    Expressioni

    Inductioni

    Activated by encephalomyocarditis virus (EMCV) and HIV-1.2 Publications

    Gene expression databases

    ArrayExpressiP61221.
    BgeeiP61221.
    CleanExiHS_ABCE1.
    GenevestigatoriP61221.

    Organism-specific databases

    HPAiCAB012476.

    Interactioni

    Subunit structurei

    Probably heterodimerizes with RNASEL; this interaction inhibits the RNASEL. Associates with HIV-1 Vif and HIV-1, HIV-2 and SIV Gag proteins.

    Protein-protein interaction databases

    BioGridi111986. 19 interactions.
    IntActiP61221. 5 interactions.
    MINTiMINT-5004380.
    STRINGi9606.ENSP00000296577.

    Structurei

    3D structure databases

    ProteinModelPortaliP61221.
    SMRiP61221. Positions 7-597.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini7 – 37314Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini46 – 75304Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini79 – 315237ABC transporter 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini342 – 562221ABC transporter 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 2 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation
    Contains 2 ABC transporter domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1245.
    HOVERGENiHBG050439.
    InParanoidiP61221.
    KOiK06174.
    OMAiRYILHAK.
    OrthoDBiEOG70GMF4.
    PhylomeDBiP61221.
    TreeFamiTF105206.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR001450. 4Fe4S-bd_dom.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR003593. AAA+_ATPase.
    IPR013283. ABC_E.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR027417. P-loop_NTPase.
    IPR007209. RNaseL-inhib_metal-bd_dom.
    [Graphical view]
    PfamiPF00005. ABC_tran. 2 hits.
    PF00037. Fer4. 1 hit.
    PF04068. RLI. 1 hit.
    [Graphical view]
    PRINTSiPR01868. ABCEFAMILY.
    SMARTiSM00382. AAA. 2 hits.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
    PS51379. 4FE4S_FER_2. 2 hits.
    PS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P61221-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADKLTRIAI VNHDKCKPKK CRQECKKSCP VVRMGKLCIE VTPQSKIAWI    50
    SETLCIGCGI CIKKCPFGAL SIVNLPSNLE KETTHRYCAN AFKLHRLPIP 100
    RPGEVLGLVG TNGIGKSTAL KILAGKQKPN LGKYDDPPDW QEILTYFRGS 150
    ELQNYFTKIL EDDLKAIIKP QYVDQIPKAA KGTVGSILDR KDETKTQAIV 200
    CQQLDLTHLK ERNVEDLSGG ELQRFACAVV CIQKADIFMF DEPSSYLDVK 250
    QRLKAAITIR SLINPDRYII VVEHDLSVLD YLSDFICCLY GVPSAYGVVT 300
    MPFSVREGIN IFLDGYVPTE NLRFRDASLV FKVAETANEE EVKKMCMYKY 350
    PGMKKKMGEF ELAIVAGEFT DSEIMVMLGE NGTGKTTFIR MLAGRLKPDE 400
    GGEVPVLNVS YKPQKISPKS TGSVRQLLHE KIRDAYTHPQ FVTDVMKPLQ 450
    IENIIDQEVQ TLSGGELQRV ALALCLGKPA DVYLIDEPSA YLDSEQRLMA 500
    ARVVKRFILH AKKTAFVVEH DFIMATYLAD RVIVFDGVPS KNTVANSPQT 550
    LLAGMNKFLS QLEITFRRDP NNYRPRINKL NSIKDVEQKK SGNYFFLDD 599
    Length:599
    Mass (Da):67,314
    Last modified:May 10, 2004 - v1
    Checksum:i5D582B62E95BC7A6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti118 – 1181T → A in CAA53972. (PubMed:7539425)Curated
    Sequence conflicti174 – 1796DQIPKA → ARFLRL in CAA53972. (PubMed:7539425)Curated
    Sequence conflicti471 – 4733ALA → RLR in CAA53972. (PubMed:7539425)Curated
    Sequence conflicti471 – 4733ALA → RLR in CAA52920. (PubMed:7539425)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti489 – 4891S → C Polymorphism confirmed at protein level. 1 Publication
    Corresponds to variant rs3816497 [ dbSNP | Ensembl ].
    VAR_068914

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76388 mRNA. Translation: CAA53972.1.
    X74987 mRNA. Translation: CAA52920.1.
    AB062293 mRNA. Translation: BAB93476.1.
    BT009779 mRNA. Translation: AAP88781.1.
    DQ148409 Genomic DNA. Translation: AAZ38723.1.
    BC016283 mRNA. Translation: AAH16283.1.
    BC016988 mRNA. Translation: AAH16988.1.
    CCDSiCCDS34071.1.
    PIRiA57017.
    S63672.
    RefSeqiNP_001035809.1. NM_001040876.1.
    NP_002931.2. NM_002940.2.
    UniGeneiHs.12013.

    Genome annotation databases

    EnsembliENST00000296577; ENSP00000296577; ENSG00000164163.
    GeneIDi6059.
    KEGGihsa:6059.
    UCSCiuc003ijx.3. human.

    Polymorphism databases

    DMDMi47117664.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    SeattleSNPs
    ABCMdb

    Database for mutations in ABC proteins

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76388 mRNA. Translation: CAA53972.1 .
    X74987 mRNA. Translation: CAA52920.1 .
    AB062293 mRNA. Translation: BAB93476.1 .
    BT009779 mRNA. Translation: AAP88781.1 .
    DQ148409 Genomic DNA. Translation: AAZ38723.1 .
    BC016283 mRNA. Translation: AAH16283.1 .
    BC016988 mRNA. Translation: AAH16988.1 .
    CCDSi CCDS34071.1.
    PIRi A57017.
    S63672.
    RefSeqi NP_001035809.1. NM_001040876.1.
    NP_002931.2. NM_002940.2.
    UniGenei Hs.12013.

    3D structure databases

    ProteinModelPortali P61221.
    SMRi P61221. Positions 7-597.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111986. 19 interactions.
    IntActi P61221. 5 interactions.
    MINTi MINT-5004380.
    STRINGi 9606.ENSP00000296577.

    PTM databases

    PhosphoSitei P61221.

    Polymorphism databases

    DMDMi 47117664.

    Proteomic databases

    MaxQBi P61221.
    PaxDbi P61221.
    PeptideAtlasi P61221.
    PRIDEi P61221.

    Protocols and materials databases

    DNASUi 6059.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000296577 ; ENSP00000296577 ; ENSG00000164163 .
    GeneIDi 6059.
    KEGGi hsa:6059.
    UCSCi uc003ijx.3. human.

    Organism-specific databases

    CTDi 6059.
    GeneCardsi GC04P146019.
    HGNCi HGNC:69. ABCE1.
    HPAi CAB012476.
    MIMi 601213. gene.
    neXtProti NX_P61221.
    PharmGKBi PA24404.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1245.
    HOVERGENi HBG050439.
    InParanoidi P61221.
    KOi K06174.
    OMAi RYILHAK.
    OrthoDBi EOG70GMF4.
    PhylomeDBi P61221.
    TreeFami TF105206.

    Miscellaneous databases

    ChiTaRSi ABCE1. human.
    GenomeRNAii 6059.
    NextBioi 23613.
    PROi P61221.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P61221.
    Bgeei P61221.
    CleanExi HS_ABCE1.
    Genevestigatori P61221.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR001450. 4Fe4S-bd_dom.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR003593. AAA+_ATPase.
    IPR013283. ABC_E.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR027417. P-loop_NTPase.
    IPR007209. RNaseL-inhib_metal-bd_dom.
    [Graphical view ]
    Pfami PF00005. ABC_tran. 2 hits.
    PF00037. Fer4. 1 hit.
    PF04068. RLI. 1 hit.
    [Graphical view ]
    PRINTSi PR01868. ABCEFAMILY.
    SMARTi SM00382. AAA. 2 hits.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    PROSITEi PS00198. 4FE4S_FER_1. 1 hit.
    PS51379. 4FE4S_FER_2. 2 hits.
    PS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of a RNase L inhibitor. A new component of the interferon-regulated 2-5A pathway."
      Bisbal C., Martinand C., Silhol M., Lebleu B., Salehzada T.
      J. Biol. Chem. 270:13308-13317(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, INTERACTION WITH RNASEL.
    2. "Chromosomal localization and expression pattern of the RNase L inhibitor gene."
      Aubry F., Mattei M.-G., Barque J.-P., Galibert F.
      FEBS Lett. 381:135-139(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Identification of immuno-peptidmics that are recognized by tumor-reactive CTL generated from TIL of colon cancer patients."
      Shichijo S., Itoh K.
      Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Colon adenocarcinoma.
    4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. SeattleSNPs variation discovery resource
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Duodenum and Uterus.
    7. "RNase L inhibitor (RLI) antisense constructions block partially the down regulation of the 2-5A/RNase L pathway in encephalomyocarditis-virus-(EMCV)-infected cells."
      Martinand C., Salehzada T., Silhol M., Lebleu B., Bisbal C.
      Eur. J. Biochem. 254:248-255(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY EMCV, FUNCTION.
    8. "RNase L inhibitor is induced during human immunodeficiency virus type 1 infection and down regulates the 2-5A/RNase L pathway in human T cells."
      Martinand C., Montavon C., Salehzada T., Silhol M., Lebleu B., Bisbal C.
      J. Virol. 73:290-296(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY HIV-1, FUNCTION.
    9. "The 2-5A/RNase L/RNase L inhibitor (RNI) pathway regulates mitochondrial mRNAs stability in interferon alpha-treated H9 cells."
      Le Roy F., Bisbal C., Silhol M., Martinand C., Lebleu B., Salehzada T.
      J. Biol. Chem. 276:48473-48482(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, FUNCTION.
    10. "Identification of a host protein essential for assembly of immature HIV-1 capsids."
      Zimmerman C., Klein K.C., Kiser P.K., Singh A.R., Firestein B.L., Riba S.C., Lingappa J.R.
      Nature 415:88-92(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HIV-1 VIF AND GAG PROTEINS.
    11. "Conservation of a stepwise, energy-sensitive pathway involving HP68 for assembly of primate lentivirus capsids in cells."
      Dooher J.E., Lingappa J.R.
      J. Virol. 78:1645-1656(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HIV-2 AND SIV GAG PROTEINS.
    12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "Detection and validation of non-synonymous coding SNPs from orthogonal analysis of shotgun proteomics data."
      Bunger M.K., Cargile B.J., Sevinsky J.R., Deyanova E., Yates N.A., Hendrickson R.C., Stephenson J.L. Jr.
      J. Proteome Res. 6:2331-2340(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT CYS-489, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiABCE1_HUMAN
    AccessioniPrimary (citable) accession number: P61221
    Secondary accession number(s): O88793
    , Q13181, Q13864, Q6NR76, Q96AL0, Q96B10, Q99K66
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2004
    Last sequence update: May 10, 2004
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    The ABC transporter domains seem not to be functional.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 4
      Human chromosome 4: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3