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P61221

- ABCE1_HUMAN

UniProt

P61221 - ABCE1_HUMAN

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Protein

ATP-binding cassette sub-family E member 1

Gene

ABCE1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Antagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) by RNase L through direct interaction with RNase L and therefore inhibits its endoribonuclease activity. May play a central role in the regulation of mRNA turnover. Antagonizes the anti-viral effect of the interferon-regulated 2-5A/RNase L pathway. May act as a chaperone for post-translational events during HIV-1 capsid assembly.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi110 – 1178ATP 1PROSITE-ProRule annotation
Nucleotide bindingi379 – 3868ATP 2PROSITE-ProRule annotation

GO - Molecular functioni

  1. ATPase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. iron-sulfur cluster binding Source: InterPro
  4. ribonuclease inhibitor activity Source: ProtInc

GO - Biological processi

  1. negative regulation of catalytic activity Source: GOC
  2. response to virus Source: ProtInc
  3. RNA catabolic process Source: ProtInc
  4. viral process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Host-virus interaction

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-binding cassette sub-family E member 1
Alternative name(s):
2'-5'-oligoadenylate-binding protein
HuHP68
RNase L inhibitor
Ribonuclease 4 inhibitor
Short name:
RNS4I
Gene namesi
Name:ABCE1
Synonyms:RLI, RNASEL1, RNASELI, RNS4I
ORF Names:OK/SW-cl.40
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 4

Organism-specific databases

HGNCiHGNC:69. ABCE1.

Subcellular locationi

Cytoplasm 1 Publication. Mitochondrion 1 Publication
Note: Localized to clusters of virus formation at the plasma membrane.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. membrane Source: UniProtKB
  3. mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA24404.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 599599ATP-binding cassette sub-family E member 1PRO_0000093316Add
BLAST

Proteomic databases

MaxQBiP61221.
PaxDbiP61221.
PeptideAtlasiP61221.
PRIDEiP61221.

PTM databases

PhosphoSiteiP61221.

Expressioni

Inductioni

Activated by encephalomyocarditis virus (EMCV) and HIV-1.2 Publications

Gene expression databases

BgeeiP61221.
CleanExiHS_ABCE1.
ExpressionAtlasiP61221. baseline and differential.
GenevestigatoriP61221.

Organism-specific databases

HPAiCAB012476.

Interactioni

Subunit structurei

Probably heterodimerizes with RNASEL; this interaction inhibits the RNASEL. Associates with HIV-1 Vif and HIV-1, HIV-2 and SIV Gag proteins.

Protein-protein interaction databases

BioGridi111986. 23 interactions.
IntActiP61221. 5 interactions.
MINTiMINT-5004380.
STRINGi9606.ENSP00000296577.

Structurei

3D structure databases

ProteinModelPortaliP61221.
SMRiP61221. Positions 7-597.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 37314Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini46 – 75304Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd
BLAST
Domaini79 – 315237ABC transporter 1PROSITE-ProRule annotationAdd
BLAST
Domaini342 – 562221ABC transporter 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation
Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1245.
GeneTreeiENSGT00390000015089.
HOVERGENiHBG050439.
InParanoidiP61221.
KOiK06174.
OMAiRYILHAK.
OrthoDBiEOG70GMF4.
PhylomeDBiP61221.
TreeFamiTF105206.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR003593. AAA+_ATPase.
IPR013283. ABC_E.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
IPR007209. RNaseL-inhib_metal-bd_dom.
[Graphical view]
PfamiPF00005. ABC_tran. 2 hits.
PF00037. Fer4. 1 hit.
PF04068. RLI. 1 hit.
[Graphical view]
PRINTSiPR01868. ABCEFAMILY.
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 2 hits.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P61221-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADKLTRIAI VNHDKCKPKK CRQECKKSCP VVRMGKLCIE VTPQSKIAWI
60 70 80 90 100
SETLCIGCGI CIKKCPFGAL SIVNLPSNLE KETTHRYCAN AFKLHRLPIP
110 120 130 140 150
RPGEVLGLVG TNGIGKSTAL KILAGKQKPN LGKYDDPPDW QEILTYFRGS
160 170 180 190 200
ELQNYFTKIL EDDLKAIIKP QYVDQIPKAA KGTVGSILDR KDETKTQAIV
210 220 230 240 250
CQQLDLTHLK ERNVEDLSGG ELQRFACAVV CIQKADIFMF DEPSSYLDVK
260 270 280 290 300
QRLKAAITIR SLINPDRYII VVEHDLSVLD YLSDFICCLY GVPSAYGVVT
310 320 330 340 350
MPFSVREGIN IFLDGYVPTE NLRFRDASLV FKVAETANEE EVKKMCMYKY
360 370 380 390 400
PGMKKKMGEF ELAIVAGEFT DSEIMVMLGE NGTGKTTFIR MLAGRLKPDE
410 420 430 440 450
GGEVPVLNVS YKPQKISPKS TGSVRQLLHE KIRDAYTHPQ FVTDVMKPLQ
460 470 480 490 500
IENIIDQEVQ TLSGGELQRV ALALCLGKPA DVYLIDEPSA YLDSEQRLMA
510 520 530 540 550
ARVVKRFILH AKKTAFVVEH DFIMATYLAD RVIVFDGVPS KNTVANSPQT
560 570 580 590
LLAGMNKFLS QLEITFRRDP NNYRPRINKL NSIKDVEQKK SGNYFFLDD
Length:599
Mass (Da):67,314
Last modified:May 10, 2004 - v1
Checksum:i5D582B62E95BC7A6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti118 – 1181T → A in CAA53972. (PubMed:7539425)Curated
Sequence conflicti174 – 1796DQIPKA → ARFLRL in CAA53972. (PubMed:7539425)Curated
Sequence conflicti471 – 4733ALA → RLR in CAA53972. (PubMed:7539425)Curated
Sequence conflicti471 – 4733ALA → RLR in CAA52920. (PubMed:7539425)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti489 – 4891S → C Polymorphism confirmed at protein level. 1 Publication
Corresponds to variant rs3816497 [ dbSNP | Ensembl ].
VAR_068914

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X76388 mRNA. Translation: CAA53972.1.
X74987 mRNA. Translation: CAA52920.1.
AB062293 mRNA. Translation: BAB93476.1.
BT009779 mRNA. Translation: AAP88781.1.
DQ148409 Genomic DNA. Translation: AAZ38723.1.
BC016283 mRNA. Translation: AAH16283.1.
BC016988 mRNA. Translation: AAH16988.1.
CCDSiCCDS34071.1.
PIRiA57017.
S63672.
RefSeqiNP_001035809.1. NM_001040876.1.
NP_002931.2. NM_002940.2.
UniGeneiHs.12013.

Genome annotation databases

EnsembliENST00000296577; ENSP00000296577; ENSG00000164163.
GeneIDi6059.
KEGGihsa:6059.
UCSCiuc003ijx.3. human.

Polymorphism databases

DMDMi47117664.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

SeattleSNPs
ABCMdb

Database for mutations in ABC proteins

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X76388 mRNA. Translation: CAA53972.1 .
X74987 mRNA. Translation: CAA52920.1 .
AB062293 mRNA. Translation: BAB93476.1 .
BT009779 mRNA. Translation: AAP88781.1 .
DQ148409 Genomic DNA. Translation: AAZ38723.1 .
BC016283 mRNA. Translation: AAH16283.1 .
BC016988 mRNA. Translation: AAH16988.1 .
CCDSi CCDS34071.1.
PIRi A57017.
S63672.
RefSeqi NP_001035809.1. NM_001040876.1.
NP_002931.2. NM_002940.2.
UniGenei Hs.12013.

3D structure databases

ProteinModelPortali P61221.
SMRi P61221. Positions 7-597.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 111986. 23 interactions.
IntActi P61221. 5 interactions.
MINTi MINT-5004380.
STRINGi 9606.ENSP00000296577.

PTM databases

PhosphoSitei P61221.

Polymorphism databases

DMDMi 47117664.

Proteomic databases

MaxQBi P61221.
PaxDbi P61221.
PeptideAtlasi P61221.
PRIDEi P61221.

Protocols and materials databases

DNASUi 6059.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000296577 ; ENSP00000296577 ; ENSG00000164163 .
GeneIDi 6059.
KEGGi hsa:6059.
UCSCi uc003ijx.3. human.

Organism-specific databases

CTDi 6059.
GeneCardsi GC04P146019.
HGNCi HGNC:69. ABCE1.
HPAi CAB012476.
MIMi 601213. gene.
neXtProti NX_P61221.
PharmGKBi PA24404.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG1245.
GeneTreei ENSGT00390000015089.
HOVERGENi HBG050439.
InParanoidi P61221.
KOi K06174.
OMAi RYILHAK.
OrthoDBi EOG70GMF4.
PhylomeDBi P61221.
TreeFami TF105206.

Miscellaneous databases

ChiTaRSi ABCE1. human.
GenomeRNAii 6059.
NextBioi 23613.
PROi P61221.
SOURCEi Search...

Gene expression databases

Bgeei P61221.
CleanExi HS_ABCE1.
ExpressionAtlasi P61221. baseline and differential.
Genevestigatori P61221.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR003593. AAA+_ATPase.
IPR013283. ABC_E.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
IPR007209. RNaseL-inhib_metal-bd_dom.
[Graphical view ]
Pfami PF00005. ABC_tran. 2 hits.
PF00037. Fer4. 1 hit.
PF04068. RLI. 1 hit.
[Graphical view ]
PRINTSi PR01868. ABCEFAMILY.
SMARTi SM00382. AAA. 2 hits.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
PROSITEi PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 2 hits.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a RNase L inhibitor. A new component of the interferon-regulated 2-5A pathway."
    Bisbal C., Martinand C., Silhol M., Lebleu B., Salehzada T.
    J. Biol. Chem. 270:13308-13317(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, INTERACTION WITH RNASEL.
  2. "Chromosomal localization and expression pattern of the RNase L inhibitor gene."
    Aubry F., Mattei M.-G., Barque J.-P., Galibert F.
    FEBS Lett. 381:135-139(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Identification of immuno-peptidmics that are recognized by tumor-reactive CTL generated from TIL of colon cancer patients."
    Shichijo S., Itoh K.
    Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Colon adenocarcinoma.
  4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. SeattleSNPs variation discovery resource
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Duodenum and Uterus.
  7. "RNase L inhibitor (RLI) antisense constructions block partially the down regulation of the 2-5A/RNase L pathway in encephalomyocarditis-virus-(EMCV)-infected cells."
    Martinand C., Salehzada T., Silhol M., Lebleu B., Bisbal C.
    Eur. J. Biochem. 254:248-255(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY EMCV, FUNCTION.
  8. "RNase L inhibitor is induced during human immunodeficiency virus type 1 infection and down regulates the 2-5A/RNase L pathway in human T cells."
    Martinand C., Montavon C., Salehzada T., Silhol M., Lebleu B., Bisbal C.
    J. Virol. 73:290-296(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY HIV-1, FUNCTION.
  9. "The 2-5A/RNase L/RNase L inhibitor (RNI) pathway regulates mitochondrial mRNAs stability in interferon alpha-treated H9 cells."
    Le Roy F., Bisbal C., Silhol M., Martinand C., Lebleu B., Salehzada T.
    J. Biol. Chem. 276:48473-48482(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, FUNCTION.
  10. "Identification of a host protein essential for assembly of immature HIV-1 capsids."
    Zimmerman C., Klein K.C., Kiser P.K., Singh A.R., Firestein B.L., Riba S.C., Lingappa J.R.
    Nature 415:88-92(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HIV-1 VIF AND GAG PROTEINS.
  11. "Conservation of a stepwise, energy-sensitive pathway involving HP68 for assembly of primate lentivirus capsids in cells."
    Dooher J.E., Lingappa J.R.
    J. Virol. 78:1645-1656(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HIV-2 AND SIV GAG PROTEINS.
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Detection and validation of non-synonymous coding SNPs from orthogonal analysis of shotgun proteomics data."
    Bunger M.K., Cargile B.J., Sevinsky J.R., Deyanova E., Yates N.A., Hendrickson R.C., Stephenson J.L. Jr.
    J. Proteome Res. 6:2331-2340(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT CYS-489, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiABCE1_HUMAN
AccessioniPrimary (citable) accession number: P61221
Secondary accession number(s): O88793
, Q13181, Q13864, Q6NR76, Q96AL0, Q96B10, Q99K66
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: October 29, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

The ABC transporter domains seem not to be functional.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3